liu.seSearch for publications in DiVA
1234 51 - 100 of 161
rss atomLink to result list
Permanent link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
  • Public defence: 2019-04-29 09:00 K3, Norrköping
    Holstein, Jane
    Linköping University, Department of Social and Welfare Studies, Division of Occupational Therapy. Linköping University, Faculty of Medicine and Health Sciences.
    Cultural Competence for Health Professionals: Instrument Development2019Licentiate thesis, comprehensive summary (Other academic)
    Abstract [en]

    In recent decades, both global migration in general and specifically migration to Sweden have increased. This development compels the need for delivering healthcare to the increasingly diverse populations in Sweden. To support health professionals, for instance occupational therapists, in developing their professional knowledge in encounters with foreign-born clients a self-rating instrument measuring cultural competence is developed. This may contribute to the development of suitable services for foreignborn clients and improve person-centered interventions for these clients.

    The general aim of this thesis was to develop an instrument for health professionals by examining psychometric properties and utility of the Swedish version of the Cultural Competence Assessment Instrument (CCAI-S) among occupational therapists. The specific aim of study I was to evaluate the content validity and utility of the Swedish version of the Cultural Competence Assessment Instrument (CCAI-S) among occupational therapists. The study had a descriptive and explorative design. Nineteen occupational therapists participated, divided into four focus groups. Qualitative content analysis was used to examine the content validity and utility of the CCAI-S. The specific aim of study II was to examine the clinical relevance, construct validity and reliability of the Swedish version of the Cultural Competence Assessment Instrument (CCAI-S) among Swedish occupational therapists. The study had a cross-sectional design. A web-based questionnaire was e-mailed to a randomised sample of 428 occupational therapists to investigate the construct validity, reliability and utility of the CCAI-S. Factor analysis was performed as well as descriptive statistics.

    The findings from study I revealed high content validity for all 24 items. However, six items needed reformulations and exemplifications. Regarding utility, the results showed strong support for CCAI-S. The category ‘Interactions with clients’ showed that the CCAI-S could be utilised individually for the health professional and create a higher awareness of cultural questions in practice. The category ‘Workplace and its organisational support’ displayed potential for use in different workplaces regarding CCAI-S and indicated the importance of organisational support for health professionals in the development of cultural competence. The findings from study II regarding construct validity generated a three-factor model with the labels ‘Openness and awareness’, ‘Workplace support’ and ‘Interaction skills’. All three factors showed high factor loadings and contained 12 of the 24 original items. The Cronbach’s Alpha showed high support for the three-factor model. Concerning utility, the participants reported that all 24 items had high clinical relevance.

    In conclusion, the findings from the two studies indicated good measurement properties and high clinical relevance for the CCAI-S. This may sup-port the utilisation of CCAI-S in the Swedish context for health professionals, for instance occupational therapists. The results of the instrument development show that the upcoming published version of the CCAI-S can be a valuable self-assessment tool for health professionals who strive to improve in person-centred communication in encounters with foreign-born clients. CCAI-S can also be of support for the organisation to serve as a guide for what to focus on to develop cultural competence within the staff. Altogether this presumably influence the effectiveness of the healthcare and enhance the evidence of interventions for foreign-born clients. To develop an instrument is an iterative process requiring several evaluations and tests in various settings and populations. Therefore further psychometric testing and utility studies on the CCAI-S is crucial.

    List of papers
    1. Validity and utility of the Swedish version of the Cultural Competence Assessment Instrument
    Open this publication in new window or tab >>Validity and utility of the Swedish version of the Cultural Competence Assessment Instrument
    2019 (English)In: British Journal of Occupational Therapy, ISSN 0308-0226, E-ISSN 1477-6006Article in journal (Refereed) Epub ahead of print
    Abstract [en]

    Introduction: Global migration as well as migration to Sweden has increased during the last few decades. A self-rating instrument that measures cultural competence could support occupational therapists' professional knowledge when they encounterclients from different cultural backgrounds. The purpose of this study was to evaluate the content validity and utility of the Swedish version of the Cultural Competence Assessment Instrument among occupational therapists. Nineteen occupational therapists participated in four focus groups.

    Method: Qualitative analysis was used to evaluate content validity and utility.

    Results: The results revealed that all 24 items of the Cultural Competence Assessment Instrument were valid, even though six of the items were in need of reformulations and exemplifications. The category Interactions with clients showed that the Cultural Competence Assessment Instrument – Swedish version could be utilised individually to raise awareness on cultural issues inpractice. The category Workplace and its organisational support showed that the Cultural Competence Assessment Instrument – Swedish version had potential for use in different workplaces, and indicated the importance of organisational support in the development of communications and cultural competence.

    Conclusion: The evaluation indicated positive content validity for the Cultural Competence Assessment Instrument – Swedish version, and that it had the potential to be utilised in the Swedish context.

    Keywords
    Instrument development, occupational therapy, qualitative research, ethnicity, cultural competence
    National Category
    Occupational Therapy
    Identifiers
    urn:nbn:se:liu:diva-156137 (URN)10.1177/0308022619825813 (DOI)
    Available from: 2019-04-05 Created: 2019-04-05 Last updated: 2019-04-05
  • Öberg, Eric
    et al.
    Linköping University, Faculty of Science & Engineering. Linköping University, Department of Electrical Engineering, Integrated Circuits and Systems.
    Kindeskog, Gustav
    Linköping University, Faculty of Science & Engineering. Linköping University, Department of Electrical Engineering, Integrated Circuits and Systems.
    16 GS/s Continuous-Time ΣΔ Modulator in a 22 nm SOI Process: a Simulation and Feasibility Study2018Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
    Abstract [en]

    With a reference specification model in terms of 8 GS/s Sigma Delta Modulator in a 28 nm CMOS process consuming 890 mW, the purpose with this thesis is to construct a similar and simpler model but with higher specification demands. In a 22 nm SOI process with an input signal bandwidth of 500 MHz sampled at 16 GS/s with a power consumption below 2 W, the objective is to design a Continuous-Time Sigma Delta Modulator with verified simulated functionality on a transistor level basis. This specification is accomplished - with a power consumption in total of 75 mW.

    The design methodology is divided into an integrator part along with a quantizer and feedback DAC part. A top-down strategy is carried out starting with an ideal high level Verilog-A model for the complete system, followed by a hardware implementation on transistor level.

  • Ersson, Lucas
    Linköping University, Department of Management and Engineering, Information Systems. Linköping University, Faculty of Arts and Sciences.
    Facilitating More Frequent Updates: Towards Evergreen: A Case Study of an Enterprise Software Vendor’s Response to the Emerging DevOps Trend, Drawing on Neo-Institutional Theory2018Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
    Abstract [en]

    The last couple of years the trend within the software industry has been to releasesmaller software updates more frequent, to overcome challenges and increase flexibility, to alignwith the swiftly changing industry environment. As an effect, we now see companies moving over tocapitalizing on subscriptions and incremental releases instead of charging for upgrades. By utilizingneo-institutional theory and Oliver’s (1991) strategic response theory, an enterprise systemsvendor’s response to the emerging DevOps trend can be determined.

  • Public defence: 2019-04-26 09:00 Linden, Linköping
    Anskär, Eva
    Linköping University, Department of Medical and Health Sciences. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Primärvårdscentrum, Vårdcentralen Mantorp.
    Time flies in primary care: a study on time utilisation and perceived psychosocial work environment2019Licentiate thesis, comprehensive summary (Other academic)
    Abstract [en]

    Background: Time utilisation among primary care professionals has been affected by structural changes and reorganisation performed in Swedish primary care over several decades. The work situation is complex with a heavy administrative work load. The overall aim with this thesis was to describe time utilisation among staff in Swedish primary care and to investigate associations with perceived psychosocial work environment and legitimacy of work tasks.

    Methods: A multicentre, descriptive, cross-sectional study design was used including all staff categories in primary care i.e. registered nurses, primary care physicians, care administrators, nurse assistants and allied professionals (physiotherapists, occupational therapists, psychologists, counsellors, dieticians and chiropodists) at eleven primary care centres located in southeast Sweden. The data collection consisted of a questionnaire including a subjective estimate of workload, the Bern Illegitimate Tasks Scale (BITS) and the Copenhagen Psychosocial Questionnaire (COPSOQ). Also, a time study was performed, where the participants reported their work time based on three main categories; direct patient work tasks, indirect patient work tasks and other work tasks, each with a number of subcategories. The participants reported time spent on different work tasks, day by day during two separate weeks. Response rates were 75% for the questionnaire and 79% for the time study.

    Results: In paper I the time study revealed that health professionals at the primary care centres spent 37% of their work time with direct patient work tasks. All professions estimated a higher proportion of time spent directly with patients than they reported in the time study. Physicians scored highest on the psychosocial scales of quantitative demands, stress and role conflicts. The proportion of administrative work tasks was associated with role conflicts, the more administration the more role conflicts. Findings in paper II were that more than a quarter of physicians scored above the cut-off value for BITS regarding unnecessary work tasks, which was significantly more than the proportion observed in all other professions in the survey. Across all staff groups, a perception of having to perform illegitimate work tasks was associated with experiencing negative psychosocial work environment and with high proportion of administrative-related work tasks.

    Conclusions: Swedish primary care staff spend a limited proportion of their work time directly with patients and primary care physicians perceive the psychosocial work environment in negative terms to a greater extent than all other staff members. Allocation of work tasks has an influence on the perceived psychosocial work environment. The perception of having a large number of illegitimate work tasks affects the psychosocial work environment negatively, which might influence the perception the staff have of their professional roles. Perception of high proportion of unreasonable work tasks is associated with a high proportion of non-patient-related administration.

    This thesis illuminates the importance of decision makers thoroughly considering the distribution and allocation of non-patient related work tasks among staff in primary care, in order to achieve efficient use of personnel resources and favourable working conditions. Hopefully, the results of this study will contribute to further development of primary care so that medical competence will benefit patients as much as possible.

    List of papers
    1. Time utilization and perceived psychosocial work environment among staff in Swedish primary care settings
    Open this publication in new window or tab >>Time utilization and perceived psychosocial work environment among staff in Swedish primary care settings
    2018 (English)In: BMC Health Services Research, ISSN 1472-6963, E-ISSN 1472-6963, Vol. 18, article id 166Article in journal (Refereed) Published
    Abstract [en]

    Background: Over the past decades, reorganizations and structural changes in Swedish primary care have affected time utilization among health care professionals. Consequently, increases in administrative tasks have substantially reduced the time available for face-to-face consultations. This study examined how work-time was utilized and the association between work time utilization and the perceived psychosocial work environment in Swedish primary care settings. Methods: This descriptive, multicentre, cross-sectional study was performed in 2014-2015. Data collection began with questionnaire. In the first section, respondents were asked to estimate how their workload was distributed between patients (direct and indirect patient work) and other work tasks. The questionnaire also comprised the Copenhagen Psychosocial Questionnaire, which assessed the psychosocial work environment. Next a time study was conducted where the participants reported their work-time based on three main categories: direct patient-related work, indirect patient-related work, and other work tasks. Each main category had a number of subcategories. The participants recorded the time spent (minutes) on each work task per hour, every day, for two separate weeks. Eleven primary care centres located in southeast Sweden participated. All professionals were asked to participate (n = 441), including registered nurses, primary care physicians, care administrators, nurse assistants, and allied professionals. Response rates were 75% and 79% for the questionnaires and the time study, respectively. Results: All health professionals allocated between 30.9% - 37.2% of their work-time to each main category: direct patient work, indirect patient work, and other work. All professionals estimated a higher proportion of time spent in direct patient work than they reported in the time study. Physicians scored highest on the psychosocial scales of quantitative demands, stress, and role conflicts. Among allied professionals, the proportion of work-time spent on administrative tasks was associated with more role conflicts. Younger staff perceived more adverse working conditions than older staff. Conclusions: This study indicated that Swedish primary care staff spent a limited proportion of their work time directly with patients. PCPs seemed to perceive their work environment in negative terms to a greater extent than other staff members. This study showed that work task allocations influenced the perceived psychosocial work environment.

    Place, publisher, year, edition, pages
    BIOMED CENTRAL LTD, 2018
    Keywords
    Work-time allocation; Primary care; Occupational health; Organization and administration; Stress
    National Category
    Health Care Service and Management, Health Policy and Services and Health Economy
    Identifiers
    urn:nbn:se:liu:diva-147117 (URN)10.1186/s12913-018-2948-6 (DOI)000426855700008 ()29514637 (PubMedID)
    Note

    Funding Agencies|Medical Research Council of Southeast Sweden; Sodertorn University Sweden

    Available from: 2018-04-20 Created: 2018-04-20 Last updated: 2019-04-18
  • Andraey, Rebecka
    Linköping University, Faculty of Arts and Sciences. Linköping University, Department of Management and Engineering, Commercial and Business Law.
    De nya ränteavdragsbegränsningsreglerna: Reglernas effekt på en koncerns resultatutjämningsmöjligheter utifrån ett neutralitetsperspektiv2019Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
    Abstract [sv]

    Den 1 januari 2019 trädde nya skatteregler för företagssektorn i kraft i Sverige för att förhindra skattebaserosion till följd av skatteplanering med ränteavdrag samt en ökad neutralitet mellan eget och lånat kapital. Den nya regleringen innehåller en generell ränteavdragsbegränsningsregel som återfinns i 24 kap. 24 § IL. Regeln stadgar att ett bolags negativa räntenetto får dras av upp till maximalt 30 procent av avdragsunderlaget. Den nya regleringen baseras på ett EU-direktiv om skatteundandragande och till stor del stämmer de nya ränteavdragsbegränsningsreglerna överens med vad som stadgas i EU-direktivets artikel fyra. OECD har även lämnat rekommendationer mot skattebaserodering och vinstförflyttning, vari ränteavdragsbegränsningar föreslås i deras åtgärdsplan fyra. I 24 kap. 24 – 29 §§ IL beskrivs det hur avdragsutrymmet beräknas, hur kvarstående negativa räntenetton ska hanteras samt ges en möjlighet för koncerner att kvitta positiva räntenetton mot negativa räntenetton. Problemen som uppdagas med de nya reglerna är att de särskilt kommer påverka koncerner negativt. Detta beror dels på att det är stora multinationella koncerner som primärt är avsedda att träffas av reglerna och dels på grund av att koncernbidragssystemet inte längre kommer kunna fungera helt i enlighet med sitt syfte, det vill säga att skapa skattemässig neutralitet mellan verksamhet som bedrivs i koncernform och verksamhet som bedrivs i ett bolag. Denna effekt grundar sig i att avdragsunderlag riskerar att ”fastna” i det koncernbidragsgivande bolaget, till följd av turordningen för tillämpning av koncernbidragsreglerna i 35 kap. IL och de nya ränteavdragsbegränsningsreglerna. En annan effekt som förväntas drabba koncerner i högre utsträckning är att de riskerar att permanent gå miste om sina negativa räntenetton. Det uppdagas även att underskottsbolag riskerar att koncernbidragsspärra sitt egenupparbetade underskott om de inte kan få avdrag för sitt negativa räntenetto.  

    Det faktum att reglerna påverkar koncerner särskilt negativt kan ifrågasättas ur ett neutralitetsperspektiv med utgångspunkten att skatteregler inte ska påverka den skattskyldiges val av handlingsalternativ. Vidare kan de nya reglerna diskuteras ur ett neutralitetsperspektiv som baseras på den nationalekonomiska tanken om att bäst resursallokering uppnås om skattereglerna är så neutrala som möjligt. Detta med hänsyn till förarbetenas fokus på ökad samhällseffektivitet, till följd av att reglerna har kombinerats med en generell sänkning av bolagsskattesatsen från 22 till 20,6 procent. Däremot har det inte fokuserats särskilt mycket på effekterna på samhällseffektiviteten vad avser det faktum att lånat kapital genom reglerna blir dyrare. De nya reglerna kan inte motiveras ur ett neutralitetsperspektiv vad avser valet mellan att bedriva sin verksamhet i ett eller flera bolag. Däremot är ökad neutralitet mellan eget och lånat kapital ett önskvärt syfte som åstadkoms genom lagstiftningen, liksom minskad möjlighet till skatteplanering med ränteavdrag. Särskilda undantagsregler för att mildra effekterna för koncerner har dock tillåtits i direktivet, samt föreslagits i OECD:s rekommendationer. Det är därav överraskande att Sverige har valt att inte anta sådana regler, med tanke på hur mycket hårdare den nya regleringen slår på de koncerner som träffas av den. Neutralitet mellan eget och lånat kapital samt undvikande av skattebaserosion och breddade skattebaser är samtliga önskvärda och legitima målsättningar, som skulle kunna rättfärdiga ett eventuellt avsteg från neutralitet mellan enskilda bolag och koncerner. Däremot är det svårt att rättfärdiga avsteget i förevarande fall, eftersom det inte har införts tillåtna undantagsregler för koncerner i lagstiftningen. Med andra ord, målen hade kunnat åstadkommas fast på en mindre bekostnad av neutraliteten mellan verksamhet som bedrivs i koncernform och verksamhet som bedrivs i ett enda bolag. Det ifrågasätts även om lagstiftaren, genom nya regler, verkligen borde få urholka effektiviteten i tidigare regler. Med detta avses hur ränteavdragsbegränsningarna påverkar koncernbidragssystemets möjlighet att vara effektiv i förhållande till sitt syfte. 

  • Tensetti, Louise
    Linköping University, Faculty of Arts and Sciences. Linköping University, Department of Management and Engineering, Commercial and Business Law.
    AI i styrelserummet: Artificiell intelligens i aktiebolagsrättsligt sammanhang2019Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
    Abstract [sv]

    Artificiell intelligens är digitala tekniker som har utvecklats snabbt de senaste åren. Allt fler uppgifter som tidigare krävt mänsklig färdighet och intelligens kan numera utföras autonomt av artificiell intelligens. Till följd av dess förmågor att på ett effektivt sätt behandla stora mängder information, lära sig nya saker, göra förutsägelser och föreslå handlingsalternativ samt i varierande grad handla efter egna uppfattningar, kan artificiell intelligens utgöra ett viktigt stöd vid beslutsfattande. Artificiell intelligens kan i teorin till och med ersätta människor. Syftet med uppsatsen är att undersöka under vilka förutsättningar artificiell intelligens kan användas för beslutsfattande i aktiebolag och att utvärdera hur svensk aktiebolagsrätt förhåller sig till den tekniska utveckling som sker på området. Frågor som uppstår är om artificiell intelligens kan ersätta aktieägare respektive styrelsen, i vilken utsträckning styrelsen kan delegera beslutsfattande till artificiell intelligens samt i vilken utsträckning styrelsen kan ta hjälp av artificiell intelligens vid sitt beslutsfattande. Även om vissa frågor med självklarhet kan besvaras, visar studien att det någonstans på vägen kommer en punkt där det inte går att avgöra hur artificiell intelligens ska behandlas. Undersökningen visar att artificiell intelligens varken kan ersätta aktieägare eller styrelsen i ett aktiebolag. Vad gäller styrelsens möjlighet att delegera beslutsfattande till artificiell intelligens samt om styrelsen kan ta hjälp av artificiell intelligens vid beslutsfattande, uppstår frågan om det kan anses oaktsamt av en styrelseledamot att använda artificiell intelligens på nämnt sätt och i sådant fall under vilka förutsättningar. En sådan aktsamhetsbedömning kan leda till att styrelseledamöter sanktioneras genom skadestånd. Rättsläget är oklart avseende om de uppgifter som styrelsen kan delegera till fysiska personer eller organ inom bolaget med ansvarsreducerande verkan, även kan delegeras till AI. Slutligen har styrelsen ett mycket stort handlingsutrymme att fatta beslut i aktiebolaget, under förutsättning att styrelsen iakttar bolagets intresse med omsorg. Det torde vara upp till styrelsen att avgöra om ett ärende kan beredas på ett tillfredsställande sätt med hjälp av AI. 

  • Erlandsson, Daniel
    Linköping University, Department of Mathematics. Linköping University, Faculty of Science & Engineering.
    GeoGebra som vertyg för gymnasieelevers lärande av andragradsfunktioner: En litteraturöversikt2019Independent thesis Basic level (professional degree), 10 credits / 15 HE creditsStudent thesis
    Abstract [en]

    The use of computer based tools as GeoGebra in the classroom has an increasing impact on the teaching and learning of mathematics. The purpose of the thesis is to examine which arguments are given in the research literature in favour of using GeoGebra in the teaching of quadratic functions instead of traditional teaching in upper secondary school. The essay examines what impact GeoGebra has on the high school students' achievements and attitudes, as reported in the research literature, in the learning of quadratic functions. The results of the literature study show that use of the program supports students´ familiarity with the features and concepts of quadratic functions, and improves their achievements in this area. Furthermore, the relationship between the algebraic expression of the quadratic function and its graphic representation seems to become more transparent to the students. As a side effect the use of this program seems to create a more stimulating teaching environment, an increased interest in mathematics and a more positive attitude to mathematics overall. An important implication for teachers is that GeoGebra's synchronized views contribute to enhance students’ conceptual understanding of mathematics, which facilitates their encounter with new mathematical elements.

  • Joelsson, Karin
    Linköping University, Department of Behavioural Sciences and Learning, Education, Teaching and Learning. Linköping University, Faculty of Educational Sciences.
    Läsning på högstadiet: En kvalitativ intervjustudie med en tematisk analys av verksamma speciallärares och specialpedagogers arbete med läsning på högstadiet2018Independent thesis Advanced level (professional degree), 10 credits / 15 HE creditsStudent thesis
    Abstract [sv]

    Uppsatsens syfte är att ta reda på hur verksamma speciallärare och specialpedagoger på högstadiet beskriver sitt arbete med att utveckla högstadieelevers läsning. Vidare avser studien att undersöka hur speciallärare och specialpedagoger förhåller sig till inkludering i sitt arbete med att utveckla läsningen. De frågeställningar som ligger till grund för undersökningen avser att belysa metoder speciallärare och specialpedagoger använder för att utveckla elevers läsning på högstadiet samt i vilken utsträckning läsundervisningen är inkluderande. Studien är en kvalitativ intervjustudie med en tematisk analys av datamaterialet.

     

    Studiens resultat tyder på att läsundervisningen som bedrivs på skolorna är inkluderande i stor utsträckning och att speciallärarna och specialpedagogerna har tankar kring inkludering när det gäller läsundervisningen både organisatoriskt och innehållsmässigt. Undersökningen visar att speciallärarna och specialpedagogerna använder olika tester och pedagogiska kartläggningar för att ta reda på vilka svårigheter eleverna har kring läsning och att olika insatser kring läsning görs på individ-, grupp och organisationsnivå. Resultatet indikerar också att speciallärare och specialpedagoger på högstadiet använder olika metoder kring läsundervisningen beroende på en rad olika faktorer såsom speciallärarnas och specialpedagogernas tidigare erfarenheter av specialpedagogisk undervisning, teoretiskt kunnande, skolans elevunderlag, skolans organisatoriska förutsättningar, lärarnas inställning och samarbetsvilja kring läsundervisningen. Studien visar vidare att öka elevernas motivation gällande läsningen samt att få eleverna att utveckla lässtrategier är centralt i speciallärarnas och specialpedagogernas arbete med läsning på högstadiet. Resultatet av studien pekar på att speciallärarna och specialpedagogerna ser sig som utbildare och handledare till både elever och pedagogisk personal på högstadiet. Studiens resultat indikerar vidare att det finns ett dilemma mellan specialpedagogiska insatser och kunskapsinhämtning på högstadiet där betygen står i centrum snarare än en utveckling av läsförmågan.

  • Public defence: 2019-04-26 13:05 Originalet, Jönköping
    Gustafsson Bragde, Hanna
    Linköping University, Department of Clinical and Experimental Medicine. Linköping University, Faculty of Medicine and Health Sciences. Laboratory Medicine, Region Jönköping County.
    Biomarkers of Inflammation and Intestinal Mucosa Pathology in Celiac Disease2019Doctoral thesis, comprehensive summary (Other academic)
    Abstract [en]

    Celiac disease (CD) is a chronic small intestinal immune-mediated enteropathy triggered by gluten. The only currently available treatment is complying with a lifelong gluten-free diet, which should not be commenced before a CD diagnosis has been established by diagnostic test results (including histopathologic assessment of small intestinal biopsies and CD-specific antibody levels). This makes diagnostic swiftness and accuracy important. In cases with low CD-specific antibody levels and/or low-grade intestinal injuries the diagnosis can be difficult to establish. The main objective of this thesis was to complement and improve CD diagnostics by identifying and implementing new biomarkers, mainly based on gene expression, in small intestinal biopsies and blood. In paper I, genes were selected to reflect villous height, crypt elongation, immune response, and epithelial integrity. The results showed that a subset of those genes could discriminate active CD mucosa from mucosa without CD-related changes and grade the intestinal injury. In paper III, an unbiased investigation of gene expression in CD mucosa was performed using transcriptome analysis. Active CD and non-CD mucosa showed differential expression in a subset of genes, and some were differentially expressed in CD mucosa before histopathologic assessment could confirm intestinal alterations compatible with a CD diagnosis. Gene set analysis revealed that there are many biological processes affected in CD mucosa, including those associated with immune response, microbial infection, phagocytosis, intestinal barrier function, metabolism, and transportation.

    In parallel, gene expression was investigated in stabilised whole blood. Blood is a more accessible sampling material than intestinal biopsies, and stabilised blood is suitable for routine diagnostics since transcript levels are preserved at sampling. In paper II, expressions from a selection of genes were quantified in stabilised whole blood (RNA) and/or plasma (protein). Three genes with differential expression in CD were identified. Compared to the CD-specific autoantibodies against tissue transglutaminase (anti-TG2) alone, the addition of the information from the new potential markers resulted in a nonsignificant contribution to the diagnostic capacity of anti-TG2. An unbiased investigation using transcriptome analysis (paper IV) showed that gene level expression differences in stabilised whole blood were small between CD and non-CD. However, expression differences on a gene set level could potentially be used in CD diagnostics. CD-associated biological processes suggested by the results included a pro-inflammatory response, negative regulation of viral replication, proliferation, differentiation, cell migration, cell survival, translation, and haemostasis.

    Expression analysis using real-time polymerase chain reaction (PCR) is easy to perform, with instrumentation available at most clinical laboratories. Although select solitary biomarkers could be very useful in the diagnosis of CD, basing gene expression profiles on pathway information instead of single genes might also disclose disease heterogeneity between patients and add stability to a diagnostic method based on gene expressions. In conclusion, the results of this work demonstrate that analysing the expression of a few small intestinal genes can complement CD diagnostics. The application of gene expression analysis in cases with minor small intestine histopathological changes shows promising results, but needs further investigations. Additionally, gene expressions in other inflammatory diseases of the small intestine need to be investigated and compared with CD to complete the picture. Moreover, the findings from this work give clues about the biological contexts in which CD resides, and the potential of gene expression in blood at a gene set level is of interest for further investigations.

    List of papers
    1. Gene Expression Profiling of Duodenal Biopsies Discriminates Celiac Disease Mucosa From Normal Mucosa
    Open this publication in new window or tab >>Gene Expression Profiling of Duodenal Biopsies Discriminates Celiac Disease Mucosa From Normal Mucosa
    2011 (English)In: Pediatric Research, ISSN 0031-3998, E-ISSN 1530-0447, Vol. 69, no 6, p. 530-537Article in journal (Refereed) Published
    Abstract [en]

    Celiac disease (CD) is identified by histopathologic changes in the small intestine which normalize during a gluten-free diet. The histopathologic assessment of duodenal biopsies is usually routine but can be difficult. This study investigated gene expression profiling as a diagnostic tool. A total of 109 genes were selected to reflect alterations in crypt-villi architecture, inflammatory response, and intestinal permeability and were examined for differential expression in normal mucosa compared with CD mucosa in pediatric patients. Biopsies were classified using discriminant analysis of gene expression. Fifty genes were differentially expressed, of which eight (APOC3, CYP3A4, OCLN, MAD2L1, MKI67, CXCL11, IL17A, and CTLA4) discriminated normal mucosa from CD mucosa without classification errors using leave-one-out cross-validation (n = 39) and identified the degree of mucosal damage. Validation using an independent set of biopsies (n = 27) resulted in four discrepant cases. Biopsies from two of these cases showed a patchy distribution of lesions, indicating that discriminant analysis based on single biopsies failed to identify CD mucosa. In the other two cases, serology support class according to discriminant analysis and histologic specimens were judged suboptimal but assessable. Gene expression profiling shows promise as a diagnostic tool and for follow-up of CD, but further evaluation is needed.

    Place, publisher, year, edition, pages
    International Pediatrics Research Foundation, Inc. SN -, 2011
    National Category
    Medical Genetics
    Identifiers
    urn:nbn:se:liu:diva-156089 (URN)10.1203/PDR.0b013e318217ecec (DOI)
    Available from: 2019-04-03 Created: 2019-04-03 Last updated: 2019-04-03
    2. Potential blood-based markers of celiac disease
    Open this publication in new window or tab >>Potential blood-based markers of celiac disease
    Show others...
    2014 (English)In: BMC Gastroenterology, ISSN 1471-230X, E-ISSN 1471-230X, Vol. 14, no 176Article in journal (Refereed) Published
    Abstract [en]

    Background: Blood-based diagnostics has the potential to simplify the process of diagnosing celiac disease (CD). Although high levels of autoantibodies against tissue transglutaminase (anti-TG2) are strongly indicative of active CD, several other scenarios involve a need for additional blood-based CD markers. Methods: We investigated the levels of messenger RNA (mRNA) in whole blood (n = 49) and protein in plasma (n = 22) from cases with active CD (n = 20), with confirmed CD and normalized histology (n = 15), and without a CD diagnosis (n = 14). Group differences were analyzed using Kruskal-Wallis one-way analysis of variance by ranks. We also investigated correlations between levels of potential markers, histopathology according to the modified Marsh scale, and CD risk gradient based on HLA type, using Spearman rank correlation. The relation between HLA-DQ2 gene dose effect and the expression levels of selected blood-based markers was investigated using the Mann-Whitney U test. Finally, the diagnostic performance of anti-TG2, potential blood-based CD markers, and logistic regression models of combined markers was evaluated using receiver operating characteristic (ROC) curve analysis. Results: CXCL11 protein levels and TNFRSF9 and TNFSF13B mRNA levels were identified as potential CD markers. These are all affected by or involved in the regulation of the NF-kappa B complex. CXCL11 protein levels and IL21 and IL15 mRNA levels were correlated with histopathology according to the modified Marsh scale, as were the established CD markers. HLA genotype risk and HLA-DQ2 gene dose effect did not show any significant relations with either the potential CD markers or the established CD markers. ROC curve analysis revealed a slight, non-significant increase in the area under the curve for the combined use of anti-TG2 and different constellations of potential blood-based CD markers compared to anti-TG2 alone. Conclusions: The CD markers identified in this study further emphasize the significance of components related to NF-kappa B regulation in relation to CD. However, the relevance of CXCL11, TNFSF13B, TNFRSF9, and other NF-kappa B interacting proteins recognized by pathway analysis, needs to be further investigated in relation to diagnosis and monitoring of CD.

    Place, publisher, year, edition, pages
    BioMed Central, 2014
    Keywords
    Celiac disease; Molecular diagnostics; Blood-based biological markers
    National Category
    Clinical Medicine
    Identifiers
    urn:nbn:se:liu:diva-112037 (URN)10.1186/1471-230X-14-176 (DOI)000342782900001 ()25298177 (PubMedID)
    Note

    Funding Agencies|Futurum - the Academy for Healthcare; Jonkoping County Council; Medical Research Council of Southeast Sweden

    Available from: 2014-11-17 Created: 2014-11-13 Last updated: 2019-04-03
    3. Celiac disease biomarkers identified by transcriptome analysis of small intestinal biopsies
    Open this publication in new window or tab >>Celiac disease biomarkers identified by transcriptome analysis of small intestinal biopsies
    Show others...
    2018 (English)In: Cellular and Molecular Life Sciences (CMLS), ISSN 1420-682X, E-ISSN 1420-9071, Vol. 75, no 23, p. 4385-4401Article in journal (Refereed) Published
    Abstract [en]

    Establishing a celiac disease (CD) diagnosis can be difficult, such as when CD-specific antibody levels are just above cutoff or when small intestinal biopsies show low-grade injuries. To investigate the biological pathways involved in CD and select potential biomarkers to aid in CD diagnosis, RNA sequencing of duodenal biopsies from subjects with either confirmed Active CD (n=20) or without any signs of CD (n=20) was performed. Gene enrichment and pathway analysis highlighted contexts, such as immune response, microbial infection, phagocytosis, intestinal barrier function, metabolism, and transportation. Twenty-nine potential CD biomarkers were selected based on differential expression and biological context. The biomarkers were validated by real-time polymerase chain reaction of eight RNA sequencing study subjects, and further investigated using an independent study group (n=43) consisting of subjects not affected by CD, with a clear diagnosis of CD on either a gluten-containing or a gluten-free diet, or with low-grade intestinal injury. Selected biomarkers were able to classify subjects with clear CD/non-CD status, and a subset of the biomarkers (CXCL10, GBP5, IFI27, IFNG, and UBD) showed differential expression in biopsies from subjects with no or low-grade intestinal injury that received a CD diagnosis based on biopsies taken at a later time point. A large number of pathways are involved in CD pathogenesis, and gene expression is affected in CD mucosa already in low-grade intestinal injuries. RNA sequencing of low-grade intestinal injuries might discover pathways and biomarkers involved in early stages of CD pathogenesis.

    Place, publisher, year, edition, pages
    SPRINGER BASEL AG, 2018
    Keywords
    RNA-seq; RNA sequencing; Molecular biomarkers; Gene expression profiling; Gene ontology enrichment analysis
    National Category
    Biochemistry and Molecular Biology
    Identifiers
    urn:nbn:se:liu:diva-152799 (URN)10.1007/s00018-018-2898-5 (DOI)000449307300008 ()30097691 (PubMedID)
    Note

    Funding Agencies|Futurum-the Academy for Health and Care, Region Jonkoping County; Medical Research Council of Southeast Sweden; National Genomics Infrastructure - Swedish Research Council

    Available from: 2018-11-22 Created: 2018-11-22 Last updated: 2019-04-10
  • Bragde, Hanna
    et al.
    Division of Medical Diagnostics, Ryhov County Hospital, Sweden.
    Jansson, Ulf
    Department of Pediatrics, Ryhov County Hospital, Sweden.
    Jarlsfelt, Ingvar
    Division of Medical Diagnostics, Ryhov County Hospital, Sweden.
    Söderman, Jan
    Division of Medical Diagnostics, Ryhov County Hospital, Sweden.
    Gene Expression Profiling of Duodenal Biopsies Discriminates Celiac Disease Mucosa From Normal Mucosa2011In: Pediatric Research, ISSN 0031-3998, E-ISSN 1530-0447, Vol. 69, no 6, p. 530-537Article in journal (Refereed)
    Abstract [en]

    Celiac disease (CD) is identified by histopathologic changes in the small intestine which normalize during a gluten-free diet. The histopathologic assessment of duodenal biopsies is usually routine but can be difficult. This study investigated gene expression profiling as a diagnostic tool. A total of 109 genes were selected to reflect alterations in crypt-villi architecture, inflammatory response, and intestinal permeability and were examined for differential expression in normal mucosa compared with CD mucosa in pediatric patients. Biopsies were classified using discriminant analysis of gene expression. Fifty genes were differentially expressed, of which eight (APOC3, CYP3A4, OCLN, MAD2L1, MKI67, CXCL11, IL17A, and CTLA4) discriminated normal mucosa from CD mucosa without classification errors using leave-one-out cross-validation (n = 39) and identified the degree of mucosal damage. Validation using an independent set of biopsies (n = 27) resulted in four discrepant cases. Biopsies from two of these cases showed a patchy distribution of lesions, indicating that discriminant analysis based on single biopsies failed to identify CD mucosa. In the other two cases, serology support class according to discriminant analysis and histologic specimens were judged suboptimal but assessable. Gene expression profiling shows promise as a diagnostic tool and for follow-up of CD, but further evaluation is needed.

  • Sjoberg Bexelius, Tomas
    et al.
    Department of Medical Epidemiology and Biostatistics, Karolinska Institutet, Stockholm, Sweden; Astrid Lindgrens Children Hospital, Karolinska University Hospital, Stockholm, Sweden.
    Ahle, Margareta
    Linköping University, Department of Medical and Health Sciences, Division of Radiological Sciences. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Center for Diagnostics, Department of Radiology in Linköping.
    Elfvin, Anders
    Department of Pediatrics, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
    Björling, Oscar
    Department of Medical Epidemiology and Biostatistics, Karolinska Institutet, Stockholm, Sweden.
    Ludvigsson, Jonas F
    Department of Medical Epidemiology and Biostatistics, Karolinska Institutet, Stockholm, Sweden; Department of Pediatrics, Örebro University Hospital, Örebro University, Örebro, Sweden; Division of Epidemiology and Public Health, School of Medicine, University of Nottingham, Nottingham, UK; Department of Medicine, Columbia University College of Physicians and Surgeons, New York City, New York, USA.
    Andersson, Roland
    Linköping University, Department of Clinical and Experimental Medicine, Division of Surgery, Orthopedics and Oncology. Linköping University, Faculty of Medicine and Health Sciences. County Hospital Ryhov, Jönköping, Sweden.
    Intestinal failure after necrotising enterocolitis: incidence and risk factors in a Swedish population-based longitudinal study2018In: BMJ paediatrics open, ISSN 2399-9772, Vol. 2, no 1, article id e000316Article in journal (Refereed)
    Abstract [en]

    Paediatric intestinal failure (IF) is a disease entity characterised by gut insufficiency often related to short bowel syndrome. It is commonly caused by surgical removal of a large section of the small intestine in association with necrotising enterocolitis (NEC), which usually affects premature infants. This study investigated the incidence and risk of IF in preterm infants with or without NEC.

  • Timpka, Jonathan
    et al.
    Department of Clinical Sciences Lund, Neurology, Lund University, Lund, Sweden; 2Department of Neurology, Skåne University Hospital, Lund, Sweden.
    Dahlström, Örjan
    Linköping University, Department of Behavioural Sciences and Learning, Psychology. Linköping University, Faculty of Arts and Sciences.
    Spreco, Armin
    Linköping University, Department of Medical and Health Sciences, Division of Community Medicine. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Center for Business support and Development, Department of Health and Care Development.
    Nilsson, Maria H
    Department of Health Sciences, Lund University, Lund, Sweden; 8Memory Clinic, Skåne University Hospital, Malmö, Sweden.
    Iwarsson, Susanne
    Department of Health Sciences, Lund University, Lund, Sweden.
    Timpka, Toomas
    Linköping University, Department of Medical and Health Sciences, Division of Community Medicine. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Center for Business support and Development, Department of Health and Care Development.
    Odin, Per
    Department of Clinical Sciences Lund, Neurology, Lund University, Lund, Sweden; 2Department of Neurology, Skåne University Hospital, Lund, Sweden; 9Department of Neurology, Central Hospital, Bremerhaven, Germany.
    Reduced workforce participation 5 years prior to first Parkinsons disease sick-leave2018In: NPJ Parkinsons disease, ISSN 2373-8057, Vol. 4, article id 36Article in journal (Refereed)
    Abstract [en]

    The importance of understanding the prodromal phase of Parkinsons disease (PD) by systematic recording of prediagnostic symptoms and reductions in body functions has been highlighted. The aim of this study was to investigate whether persons later diagnosed with PD exhibit increased physician-certified sickness absence 1, 2, and 5 years prior to a first sick-leave episode attributed to PD. A case-control study was performed to analyze data from all nontrivial (exceeding 14 days) sick-leave episodes in Sweden between 2008 and 2014. The 537 incident PD sick-leave episodes were identified as PD sick-leave cases and compared to 537 sick-leave controls identified by matching age, sex, and date of the first day of the sick-leave episode. The total sickness absence and sickness absence due to musculoskeletal diagnoses were found to be increased among the PD sick-leave cases from 5 years prior to the first sick-leave episode ascribed to PD when compared to the controls. No differences between PD sick-leave cases and sick-leave controls were found with regard to mental and behavioral diagnoses. We conclude that the capacity to participate in working life is reduced already at the early prediagnostic stages of PD. This finding can be used as a basis for further research into the process of identifying individuals at risk for developing PD, particularly in combination with further investigation into biochemical, genetic, and imaging biomarkers.

  • Masterton, George
    et al.
    Linköping University, Department of Culture and Communication, Culture and Aesthetics. Linköping University, Faculty of Arts and Sciences.
    Olsson, Erik J
    Department of Philosophy, Lund University, Sweden.
    PageRanks ability to track webpage quality: reconciling Googles wisdom-of-crowds justification with the scale-free structure of the web2018In: Heliyon, ISSN 2405-8440, Vol. 4, no 11, article id e00978Article in journal (Refereed)
    Abstract [en]

    We address the fundamental question why we should use PageRank and similar link-based algorithms in search engines, if at all. In a legendary article from 1998, the Google founders gave an intriguing wisdom-of-crowds justification for PageRank according to which the latter tracks quality online. This striking suggestion stands in contrast to the view that PageRank merely tracks what is popular. However, Masterton and Olsson (2017) showed that web-ecologies generated by Google-like assumptions essentially fail to reflect the scale-free structure of the web. They pointed to attraction to popularity or a rich-get-richer effect being the likely main cause of scalefreeness. In this article, we explore dual models of linking behavior, i.e. models that combine attraction to importance (quality) with attraction to popularity. Our results, obtained through computer simulation, indicate that there exist dual models that give rise both to a wisdom-of-crowds effect for PageRank and to scale-free web-graphs, thus giving a partial vindication of the wisdom-of-crowds thesis for the real web. Future work should explore larger web-graphs as well as other aspects pertaining to the empirical plausibility of dual linking models.

  • Jakola, Asgeir Store
    et al.
    Department of Neurosurgery, Sahlgrenska University Hospital, Gothenburg, Sweden; Department of Neurosurgery, St. Olavs Hospital, Trondheim, Norway; Institute of Neuroscience and Physiology, Department of Clinical Neuroscience, University of Gothenburg, Sahlgrenska Academy, Gothenburg, Sweden.
    Werlenius, Katja
    Department of Oncology, Sahlgrenska University Hospital, Gothenburg, Sweden; Institute of Clinical Sciences, University of Gothenburg, Sahlgrenska Academy, Gothenburg, Sweden.
    Mudaisi, Munila
    Linköping University, Department of Clinical and Experimental Medicine, Division of Surgery, Orthopedics and Oncology. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Center for Surgery, Orthopaedics and Cancer Treatment, Department of Oncology.
    Hylin, Sofia
    Department of Neurology, Karolinska University Hospital, Stockholm, Sweden.
    Kinhult, Sara
    Department of Oncology, Skåne University Hospital, Lund, Sweden.
    Bartek, Jiri
    Department of Neurosurgery, Karolinska University Hospital, Stockholm, Sweden; Department of Clinical Neuroscience, Karolinska Institute, Stockholm, Sweden.
    Salvesen, Øyvind
    Department of Public Health and Nursing, Norwegian University of Science and Technology, Trondheim, Norway.
    Carlsen, Sven Magnus
    Department of Cancer Research and Molecular medicine, Norwegian University of Science and Technology, Trondheim, Norway; Department of Endocrinology, St. Olavs Hospital, Trondheim, Norway.
    Strandéus, Michael
    Department of Oncology, County Hospital Ryhov, Jönköping, Sweden.
    Lindskog, Magnus
    Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden; Section of Oncology, Akademiska University Hospital, Uppsala, Sweden.
    Löfgren, David
    Department of Oncology, Örebro University Hospital, Örebro, Sweden.
    Rydenhag, Bertil
    Department of Neurosurgery, Sahlgrenska University Hospital, Gothenburg, Sweden; Institute of Neuroscience and Physiology, Department of Clinical Neuroscience, University of Gothenburg, Sahlgrenska Academy, Gothenburg, Sweden.
    Carstam, Louise
    Department of Neurosurgery, Sahlgrenska University Hospital, Gothenburg, Sweden; Institute of Neuroscience and Physiology, Department of Clinical Neuroscience, University of Gothenburg, Sahlgrenska Academy, Gothenburg, Sweden.
    Gulati, Sasha
    Department of Neurosurgery, St. Olavs Hospital, Trondheim, Norway; Department of Neuroscience, Norwegian University of Science and Technology, Trondheim, Norway.
    Solheim, Ole
    Department of Neurosurgery, St. Olavs Hospital, Trondheim, Norway; Department of Neuroscience, Norwegian University of Science and Technology, Trondheim, Norway.
    Bartek, Jiri
    Department of Medical Biochemistry and Biophysics, Division of Genome Biology, Science for Life Laboratory,, Karolinska Institute, Stockholm, Sweden; Research Center, Danish Cancer Society, Copenhagen, Denmark.
    Solheim, Tora
    European Palliative Care Research Centre (PRC), Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, Norway; Cancer Clinic, St. Olavs Hospital, Trondheim, Norway.
    Disulfiram repurposing combined with nutritional copper supplement as add-on to chemotherapy in recurrent glioblastoma (DIRECT): Study protocol for a randomized controlled trial2018In: F1000 Research, E-ISSN 2046-1402, Vol. 7, article id 1797Article in journal (Refereed)
    Abstract [en]

    Background: Disulfiram (DSF) is a well-tolerated, inexpensive, generic drug that has been in use to treat alcoholism since the 1950s. There is now independent preclinical data that supports DSF as an anticancer agent, and experimental data suggest that copper may increase its anti-neoplastic properties. There is also some clinical evidence that DSF is a promising anticancer agent in extracranial cancers. In glioblastoma, DSF induced O 6-methylguanine methyltransferase (MGMT) inhibition may increase response to alkylating chemotherapy. A recent phase I study demonstrated the safety of DSF in glioblastoma patients when DSF was administered at doses below 500 mg/day together with chemotherapy. We plan to assess the effects of DSF combined with nutritional copper supplement (DSF-Cu) as an adjuvant to alkylating chemotherapy in glioblastoma treatment. Methods: In an academic, industry independent, multicenter, open label randomized controlled phase II/III trial with parallel group design (1:1) we will assess the efficacy and safety of DSF-Cu in glioblastoma treatment. The study will include 142 patients at the time of first recurrence of glioblastoma where salvage therapy with alkylating chemotherapy is planned. Patients will be randomized to treatment with or without DSF-Cu. Primary end-point is survival at 6 months. Secondary end-points are overall survival, progression free survival, quality of life, contrast enhancing tumor volume and safety. Discussion: There is a need to improve the treatment of recurrent glioblastoma. Results from this randomized controlled trial with DSF-Cu in glioblastoma will serve as preliminary evidence of the future role of DSF-Cu in glioblastoma treatment and a basis for design and power estimations of future studies. In this publication we provide rationale for our choices and discuss methodological issues. Trial registration: The study underwent registration in EudraCT 2016-000167-16 (Date: 30.03.2016,) and Clinicaltrials.gov NCT02678975 (Date: 31.01.2016) before initiating the study.

  • Sundbom, Per
    et al.
    Linköping University, Department of Medical and Health Sciences, Division of Cardiovascular Medicine. Linköping University, Faculty of Medicine and Health Sciences. Department of Medicine and Geriatrics, Höglandssjukhuset, Eksjö, Sweden.
    Suutari, Anne-Marie
    Department of Medicine and Geriatrics, Höglandssjukhuset, Eksjö, Sweden.
    Abdulhadi, Karim
    Department of Medicine and Geriatrics, Höglandssjukhuset, Eksjö, Sweden.
    Broda, Wojciech
    Department of Medicine and Geriatrics, Höglandssjukhuset, Eksjö, Sweden.
    Csegedi, Melinda
    Department of Medicine and Geriatrics, Höglandssjukhuset, Eksjö, Sweden.
    Salmonella enteritidis causing myocarditis in a previously healthy 22-year-old male2018In: Oxford Medical Case Reports, E-ISSN 2053-8855, Vol. 2018, no 12, p. 447-451Article in journal (Refereed)
    Abstract [en]

    Myocarditis is mostly caused by viral infections and rarely caused by bacterial pathogens, especially in immunocompetent individuals. Bacterial myocarditis due to Salmonella is rare, especially in countries with improved sanitation that minimize contamination of Salmonella typhi serotypes. We here present a case of a previous healthy 22-year-old male that came to the emergency room with chest pain. His symptoms occurred after a period of profuse diarrhea, fever and hematochezia. Magnetic resonance imaging confirmed the diagnosis of myocarditis. Stool culture was positive for Salmonella enteritidis. Myocarditis due to Salmonella is rare but may still occur in western countries. The inter-individual response to the pathogens and its virulence mechanisms and male gender is factors for developing myocarditis. We here add to the numbers of cases with myocarditis due to S. enteritidis. A higher suspicion and more frequent ECG and troponin testing might result in an increase of patients with subclinical myocarditis.

  • Horan, Sean T
    et al.
    University of California, Department of Mathematics, Irvine, California, United States; University of California, Beckman Laser Institute, Laser Microbeam and Medical Program, Irvine, Cali, United States.
    Gardner, Adam R
    University of California, Beckman Laser Institute, Laser Microbeam and Medical Program, Irvine, Cali, United States; University of California, Department of Chemical Engineering and Materials Science, Irvine, Californ, United States.
    Saager, Rolf
    Linköping University, Department of Biomedical Engineering, Division of Biomedical Engineering. Linköping University, Faculty of Science & Engineering. University of California, Beckman Laser Institute, Laser Microbeam and Medical Program, Irvine, Cali, United States.
    Durkin, Anthony J
    University of California, Beckman Laser Institute, Laser Microbeam and Medical Program, Irvine, Cali, United States; University of California, Department of Biomedical Engineering, Irvine, California, United States.
    Venugopalan, Vasan
    University of California, Beckman Laser Institute, Laser Microbeam and Medical Program, Irvine, Cali, United States; University of California, Department of Chemical Engineering and Materials Science, Irvine, Californ, United States; University of California, Department of Biomedical Engineering, Irvine, California, United States.
    Recovery of layered tissue optical properties from spatial frequency-domain spectroscopy and a deterministic radiative transport solver2018In: Journal of Biomedical Optics, ISSN 1083-3668, E-ISSN 1560-2281, Vol. 24, no 7, article id 071607Article in journal (Refereed)
    Abstract [en]

    We present a method to recover absorption and reduced scattering spectra for each layer of a two-layer turbid media from spatial frequency-domain spectroscopy data. We focus on systems in which the thickness of the top layer is less than the transport mean free path   (  0.1  −  0.8<i>l</i><sup>  *  </sup>  )  . We utilize an analytic forward solver, based upon the <i>N</i>’th-order spherical harmonic expansion with Fourier decomposition   (  SHEF<sub>N</sub>  )   method in conjunction with a multistage inverse solver. We test our method with data obtained using spatial frequency-domain spectroscopy with 32 evenly spaced wavelengths within λ  =  450 to 1000 nm on six-layered tissue phantoms with distinct optical properties. We demonstrate that this approach can recover absorption and reduced scattering coefficient spectra for both layers with accuracy comparable with current Monte Carlo methods but with lower computational cost and potential flexibility to easily handle variations in parameters such as the scattering phase function or material refractive index. To our knowledge, this approach utilizes the most accurate deterministic forward solver used in such problems and can successfully recover properties from a two-layer media with superficial layer thicknesses.

  • Public defence: 2019-04-25 13:15 TEMCAS, T-huset, Linköping
    Crusoe, Jonathan
    Linköping University, Department of Management and Engineering, Information Systems. Linköping University, Faculty of Arts and Sciences.
    Why is it so challenging to cultivate open government data?: Understanding impediments from an ecosystem perspective2019Licentiate thesis, comprehensive summary (Other academic)
    Abstract [en]

    Introduction: This compilation licentiate thesis focuses on open government data (OGD). The thesis is based on three papers. OGD is a system that is organized when publishers collect and share data with users, who can unrestrictedly reuse the data. In my research, I have explored why it can be challenging to cultivate OGD. Cultivation is human activities that change, encourage, or guide human organizations towards a higher purpose by changing, introducing, managing, or removing conditions. Here, the higher purpose is OGD to realize believed benefits. Thus, OGD cultivation is an attempt to stimulate actors into organizing as OGD.

    Problem and Purpose: OGD is believed to lead to several benefits. However, the worldwide OGD movement has slowed down, and researchers have noted a lack of use. Publishers and users are experiencing a set of different impediments that are challenging to solve. In previous research, there is a need for more knowledge about what can impede the OGD organization, cause non-valuable organizing, or even collapse the organization. At the same time, there is a lack of knowledge about how impediments shape the organization of OGD. This gap can make it hard to solve and overcome the impediments experienced by publishers and users. The sought-after knowledge can bring some understanding of the current situation of OGD. In this research, I have viewed the organization of OGD as an ecosystem. The purpose of this thesis is to draw lessons about why it can be challenging to cultivate OGD ecosystems by understanding OGD impediments from an ecosystem perspective.

    Research Design: I set out to explore OGD through qualitative research from 2016 to 2018. My research started with a pilot case study that led to three studies. The studies are each reported in a paper and the papers form the base of this thesis. The first paper aims to stimulate the conceptually oriented discussion about actors’ roles in OGD by developing a framework that was tested on a Swedish public agency. The second paper has the purpose of expanding the scope surrounding impediments and was based in a review and systematization of previous research about OGD impediments. The third paper presents an exploration of impediments experienced by publishers, users, and cultivators in the Swedish national OGD ecosystem to identify faults. From the three papers, lessons were drawn in turn and together, that are presented in this thesis.

    Findings: Cultivators when cultivating OGD ecosystems are facing towering challenges. The following three main challenges are identified in this thesis: (1) to cultivate a system that can manage stability by itself without constant involvement, (2) to cultivate a system that is capable of evolving towards a “greater good” by itself, and (3) to have an up-to-date precise vocabulary for a self-evolving system that enables inter-subjective understand for coordinating problem-solving.

    Contribution: The theoretical contribution of this thesis is that OGD ecosystems can be viewed as a public utility. Moreover, I recommend that researchers approach the organizing of OGD as the cultivation of evolution, rather than the construction of a structure; to consider the stability of the system in growth, value, and participation; and to be cautious with how they label and describe OGD actors. For actors that are cultivating OGD, I recommend that they guide the OGD actors to help them organize; view OGD cultivation as the management of evolution (growth) towards a purpose; and view cultivation as a collaborative effort where they can supply ideas, technologies, practices, and expertise.

    List of papers
    1. Investigating open government data barriers: A literature review and conceptualization
    Open this publication in new window or tab >>Investigating open government data barriers: A literature review and conceptualization
    2018 (English)In: Electronic Government: EGOV 2018 / [ed] Parycek P., Springer Verlag , 2018, p. 169-183Conference paper, Published paper (Refereed)
    Abstract [en]

    When focusing on open government data (OGD) publishing and related barriers, there are several complexities present. Largely, current research is focused on publishing and usage of OGD; and we argue that there are a need to investigate and to systematise OGD barrier research in order to understand and outline an expanded scope of the phenomenon. We expand by clarifying barriers linked to the release decision and the data’s organisational context. To investigate the OGD barriers, we conduct a systematic literature review, identifying 34 articles as a point of departure for our analysis. From these articles we create, present and discuss illustrations on historical development, barrier types, and different research focuses on OGD. When analysing the articles, we identify a focus on technical, organisational, and legal barrier types, while studies on open data usage and systems are less frequent. Our analysis also identifies some possible open data research barriers. In the article we also relate barriers to an expanded OGD process (Suitability, Release, Publish, Use, and Evaluation), identifying 46 barriers with possible linkages. The results is an expanded scope and a conceptual illustration of OGD barriers. © IFIP International Federation for Information Processing 2018.

    Place, publisher, year, edition, pages
    Springer Verlag, 2018
    Series
    Lecture Notes in Computer Science, ISSN 0302-9743, E-ISSN 1611-3349 ; 11020
    Keywords
    Barriers, Challenges, Impediments, Literature review, Myths, OGD, Open data, Open government data, Process, Risks, Government data processing, Processing, Literature reviews, Open datum, Reviews
    National Category
    Information Systems
    Identifiers
    urn:nbn:se:liu:diva-151311 (URN)10.1007/978-3-319-98690-6_15 (DOI)2-s2.0-85052893750 (Scopus ID)978-3-319-98689-0 (ISBN)978-3-319-98690-6 (ISBN)
    Conference
    EGOV 2018, Electronic Government
    Available from: 2018-09-17 Created: 2018-09-17 Last updated: 2019-04-02
  • Lindström, Ulf
    et al.
    Clinical Epidemiology Division, Department of Medicine, Karolinska Institutet, Stockholm, Sweden.
    Olofsson, Tor
    Department of Clinical Sciences, Lund University, Lund, Sweden.
    Wedrén, Sara
    Clinical Epidemiology Division, Department of Medicine, Karolinska Institutet, Stockholm, Sweden.
    Qirjazo, Ilia
    Region Östergötland, Heart and Medicine Center, Department of Rheumatology.
    Askling, Johan
    Clinical Epidemiology Division, Department of Medicine, Karolinska Institutet, Stockholm, Sweden; Clinical Epidemiology Unit, Karolinska University Hospital Solna, Stockholm, Sweden.
    Impact of extra-articular spondyloarthritis manifestations and comorbidities on drug retention of a first TNF-inhibitor in ankylosing spondylitis: a population-based nationwide study2018In: RMD Open, E-ISSN 2056-5933, Vol. 4, no 2, article id e000762Article in journal (Refereed)
    Abstract [en]

    To assess the impact of extra-articular spondyloarthritis (SpA) manifestations (anterior uveitis, psoriasis and inflammatory bowel disease (IBD)), and of comorbidities, on tumour necrosis factor alpha inhibitor (TNFi) drug retention in ankylosing spondylitis (AS).

  • Daghighi, Abtin
    et al.
    Linköping University, Department of Clinical and Experimental Medicine, Division of Surgery, Orthopedics and Oncology. Linköping University, Faculty of Medicine and Health Sciences.
    Tropp, Hans
    Linköping University, Department of Clinical and Experimental Medicine, Division of Surgery, Orthopedics and Oncology. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Center for Surgery, Orthopaedics and Cancer Treatment, Department of Orthopaedics in Linköping.
    Dahlström, Nils
    Linköping University, Department of Medical and Health Sciences, Division of Radiological Sciences. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Center for Diagnostics, Department of Radiology in Linköping. Linköping University, Center for Medical Image Science and Visualization (CMIV).
    Klarbring, Anders
    Linköping University, Department of Management and Engineering, Solid Mechanics. Linköping University, Faculty of Science & Engineering.
    Correction: F.E.M. Stress-Investigation of Scolios Apex2018In: Open Biomedical Engineering Journal, ISSN 1874-1207, E-ISSN 1874-1207, Vol. 12Article in journal (Refereed)
  • Mirdell, Robin
    et al.
    Linköping University, Department of Clinical and Experimental Medicine, Division of Surgery, Orthopedics and Oncology. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Anaesthetics, Operations and Specialty Surgery Center, Department of Hand and Plastic Surgery.
    Lemstra-Idsardi, Aukje Nienke
    University of Twente, Enschede, Netherlands.
    Farnebo, Simon
    Linköping University, Department of Clinical and Experimental Medicine, Division of Surgery, Orthopedics and Oncology. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Anaesthetics, Operations and Specialty Surgery Center, Department of Hand and Plastic Surgery.
    Tesselaar, Erik
    Linköping University, Department of Clinical and Experimental Medicine, Division of Surgery, Orthopedics and Oncology. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Center for Diagnostics, Medical radiation physics. Linköping University, Department of Medical and Health Sciences, Division of Radiological Sciences.
    Data on microcirculatory perfusion dips in the resting nail bed2018In: Data in Brief, E-ISSN 2352-3409, Vol. 21, p. 1232-1235Article in journal (Refereed)
    Abstract [en]

    This article contains the raw data from the article entitled: "The presence of synchronized perfusion dips in the microcirculation of the resting nail bed" Mirdell et al. (in press). A laser speckle contrast imager (LSCI) was used to make a total of 21 recordings of the perfusion in the resting nail bed of 10 healthy test subjects. The first 10 recordings were acquired after 5?min of acclimatization. An additional 10 recordings were acquired in the same test subjects, after 20?min of acclimatization. In the last recording, a digital nerve block was applied to the left dig III. The data show the presence of highly irregular perfusion variations, a phenomenon we like to call perfusion dips. The data also show how the perfusion dips can be abolished through a digital nerve block. An algorithm for the quantification of the perfusion dips is included in the data.

  • Busardò, Francesco Paolo
    et al.
    a Department of Anatomical, Histological, Forensic and Orthopaedic Sciences , Sapienza University of Rome , Rome , Italy.
    Jones, A Wayne
    Linköping University, Department of Medical and Health Sciences, Division of Drug Research. Linköping University, Faculty of Medicine and Health Sciences.
    Interpreting γ-hydroxybutyrate concentrations for clinical and forensic purposes2019In: Clinical Toxicology, ISSN 1556-3650, E-ISSN 1556-9519, Vol. 57, no 3, p. 149-163Article in journal (Refereed)
    Abstract [en]

    ?-Hydroxybutyric acid is an endogenous substance, a therapeutic agent, and a recreational drug of abuse. This psychoactive substance acts as a depressant of the central nervous system and is commonly encountered in clinical and forensic practice, including impaired drivers, poisoned patients, and drug-related intoxication deaths.

  • Enkirch, Theresa
    et al.
    European Programme for Public Health Microbiology Training (EUPHEM), European Centre for Disease Prevention and Control (ECDC), Stockholm, Sweden; Public Health Agency of Sweden, Solna, Sweden.
    Eriksson, Ronnie
    National Food Agency, Uppsala, Sweden.
    Persson, Sofia
    National Food Agency, Uppsala, Sweden.
    Schmid, Daniela
    Austrian Agency for Health and Food Safety, Vienna, Austria.
    Aberle, Stephan W
    Center for Virology, Medical University of Vienna, Vienna, Austria.
    Löf, Emma
    European Programme for Intervention Epidemiology Training (EPIET), European Centre for Disease Prevention and Control, (ECDC), Stockholm, Sweden; Public Health Agency of Sweden, Solna, Sweden.
    Wittesjö, Bengt
    Department of Communicable Disease Control and Prevention, Blekinge County, Sweden.
    Holmgren, Birgitta
    Department of Communicable Disease Control and Prevention, Skåne County, Sweden.
    Johnzon, Charlotte
    The Environment and Health Administration of Stockholm Municipality, Stockholm, Sweden.
    Gustafsson, Eva X
    Department of Communicable Disease Control and Prevention, Skåne County, Sweden.
    Svensson, Lena M.
    Region Östergötland, Center for Business support and Development, Department of Communicable Disease and Infection Control.
    Sandelin, Lisa Labbé
    Department of Communicable Disease Control and Prevention, Kalmar County, Sweden.
    Richter, Lukas
    Austrian Agency for Health and Food Safety, Vienna, Austria.
    Lindblad, Mats
    National Food Agency, Uppsala, Sweden.
    Brytting, Mia
    Public Health Agency of Sweden, Solna, Sweden.
    Maritschnik, Sabine
    Austrian Agency for Health and Food Safety, Vienna, Austria.
    Tallo, Tatjana
    Public Health Agency of Sweden, Solna, Sweden.
    Malm, Therese
    Department of Communicable Disease Control and Prevention, Gävleborg County, Sweden.
    Sundqvist, Lena
    Public Health Agency of Sweden, Solna, Sweden.
    Ederth, Josefine Lundberg
    Public Health Agency of Sweden, Solna, Sweden.
    Hepatitis A outbreak linked to imported frozen strawberries by sequencing, Sweden and Austria, June to September 20182018In: Eurosurveillance, ISSN 1025-496X, E-ISSN 1560-7917, Vol. 23, no 41, article id 1800528Article in journal (Refereed)
    Abstract [en]

    Between June-September 2018, 20 hepatitis A cases were notified in six counties in Sweden. Combined epidemiological and microbiological investigations identified imported frozen strawberries produced in Poland as the source of the outbreak. Sequence analysis confirmed the outbreak strain IB in the strawberries with 100 % identity and the respective batch was withdrawn. Sharing the sequence information internationally led to the identification of 14 additional cases in Austria, linked to strawberries from the same producer.

  • Krantz, Kristin (Editor)
    Linköping University, University Library.
    Verksamhetsberättelse Linköpings universitetsbibliotek (LiUB) 20182018Report (Other academic)
    Abstract [sv]

    2018 blev ett händelserikt år på biblioteket. I ljuset av att LiU lägger fokus på undervisning, livslångt lärande, e-lärande etc. började biblioteket se över sin egen undervisning. Denna översyn visade att en teambaserad lösning var lämplig att tillämpa. Nästa steg är att titta på innehållet i bibliotekets undervisning: vad är dagens behov bland studenter? Hur bidrar biblioteket till deras (livslånga) behov av att hitta pålitlig information? Kan man dra nytta av att använda mer teknik? Kan biblioteket öka insatserna för att hjälpa studenter undvika plagiering? Arbetet fortsätter.

    Nya studenthuset är ett självklart fokus på Campus Valla och eftersom inflyttning närmar sig blev arbetet med detaljer viktiga: placering, möblering, arbetssätt… Men det är inte endast Valla som tänker nytt: exempelvis håller Campus Norrköpings bibliotek på att skapa utrymme för MakerSpace och Digital Lab (DigiMaker) och på Medicinska biblioteket pågår arbete för att prova meröppet (dvs obemannade timmar på kvällen).

    Även avtal med förlag har ändrats mycket under året. Sveriges Rektorer signalerade starkt att läro-sätena vill gå mot ökad open access. Det betyder att nya avtal med förlag ofta är ”Read and Publish”, dvs man betalar extra så att universitets publikationer blir fritt tillgängliga i hela världen. Alla förlag är inte förtjusta i modellen. Elsevier har sagt nej till ett sådant avtal, så från och med 1 juli har UB därför inte haft tillgång till nya artiklar från Elsevier tidskrifter. På plussidan har detta frigjort pengar för olika open access-initiativ, som t.ex. en fond som täcker kostnaderna för de LiU-forskare som publicerar i rena OA tidskrifter.

    Och medan allt detta pågår är biblioteket självklart öppet med full service!

  • Ratanjee-Vanmali, Husmita
    et al.
    Department of Speech-Language Pathology and Audiology , University of Pretoria , Pretoria , South Africa; b Hearing Research Clinic Non-Profit Company , Durban , South Africa.
    Swanepoel, De Wet
    Department of Speech-Language Pathology and Audiology , University of Pretoria , Pretoria , South Africa; c Ear Sciences Centre , The University of Western Australia , Nedlands , Australia; d Ear Science Institute Australia , Subiaco , Australia.
    Laplante-Levesque, Ariane
    Linköping University, Department of Behavioural Sciences and Learning, Disability Research. Linköping University, Faculty of Arts and Sciences. Oticon Medical , Oticon A/S, Copenhagen , Denmark.
    Characteristics, behaviours and readiness of persons seeking hearing healthcare online2019In: International Journal of Audiology, ISSN 1499-2027, E-ISSN 1708-8186, Vol. 58, no 2, p. 107-115Article in journal (Refereed)
    Abstract [en]

    This study describes characteristics, behaviours and readiness of people who are interested in seeking hearing healthcare (HHC) online.

  • Falkenström, Fredrik
    et al.
    Linköping University, Department of Behavioural Sciences and Learning, Psychology. Linköping University, Faculty of Arts and Sciences.
    Kumar, Manasi
    Department of Psychiatry, University of Nairobi, Kenya; Honorary Research Fellow, Research Dept of Clinical Health and Educational Psychology, University College London, UK.
    Zahid, Aiysha
    Queen Marys College, Miles End, London, E1 4NS, UK.
    Kuria, Mary
    Department of Psychiatry, University of Nairobi, Nairobi, Kenya.
    Othieno, Caleb
    Department of Psychiatry, University of Nairobi, P.O. Box 19676, Nairobi, 00202, Kenya.
    Factor analysis of the Clinical Outcomes in Routine Evaluation - Outcome Measures (CORE-OM) in a Kenyan sample2018In: BMC Psychology, E-ISSN 2050-7283, Vol. 6, no 1, article id 48Article in journal (Refereed)
    Abstract [en]

    There is no generic psychotherapy outcome measure validated for Kenyan populations. The objective of this study was to test the acceptability and factor structure of the Clinical Outcomes in Routine Evaluation - Outcome Measure in patients attending psychiatric clinics at two state-owned hospitals in Nairobi.

  • Sjöholm, Hanna
    et al.
    Linköping University, Department of Medical and Health Sciences, Division of Physiotherapy. Linköping University, Faculty of Medicine and Health Sciences. Rehabilitation Centre, Region Jönköping County, Jönköping, Sweden.
    Hägg, Staffan
    Linköping University, Department of Medical and Health Sciences, Division of Drug Research. Linköping University, Faculty of Medicine and Health Sciences. Futurum, Region Jönköping County, Jönköping, Sweden.
    Nyberg, Lars
    Department of Health Sciences, Luleå University of Technology, Luleå, Sweden.
    Rolander, Bo
    Futurum, Region Jönköping County, Jönköping, Sweden.
    Kammerlind, Ann-Sofi
    Linköping University, Department of Medical and Health Sciences, Division of Physiotherapy. Linköping University, Faculty of Medicine and Health Sciences. Futurum, Region Jönköping County, Jönköping, Sweden.
    The Cone Evasion Walk test: Reliability and validity in acute stroke2019In: Physiotherapy Research International, ISSN 1358-2267, E-ISSN 1471-2865, Vol. 24, no 1Article in journal (Refereed)
    Abstract [en]

    To estimate the reliability and validity of the Cone Evasion Walk test (CEW), a new test assessing the ability to evade obstacles, in people with acute stroke.

  • De, Swades
    et al.
    Department of Electrical Engineering and Bharti School of Telecom, Indian Institute of Technology Delhi, India.
    Kaushik, K.
    Department of Electrical Engineering and Bharti School of Telecom, Indian Institute of Technology Delhi, India.
    Mishra, Deepak
    Linköping University, Department of Electrical Engineering, Communication Systems. Linköping University, Faculty of Science & Engineering.
    Stochastic Solar Harvesting Characterization for Sustainable Sensor Node Operation2019In: IET Wireless Sensor Systems, ISSN 2043-6386, p. 1-11Article in journal (Refereed)
    Abstract [en]

    Self-sustainability of low power wireless sensor nodes is the need of the hour to realize ubiquitous wireless networks. To address this requirement we investigate the practical feasibility of sustainable green sensor network with solar-powered nodes. We propose simple yet efficient (i) analytical circuit model for solar panel assisted supercapacitor charging and (ii) statistical model for characterizing the solar intensity distribution. Combining these circuit and statistical models, we derive a novel solar charging rate distribution for the solar-powered supercapacitor. To gain analytical insights, we also propose an ideal diode based tight approximation for the practical supercapacitor charging circuit model. The accuracy of these proposed analytical models have been validated by extensive numerical simulations based on the real-world data, i.e., solar intensity profile and solar panel characteristics. The derived solar charging rate distribution is used to investigate the supported sampling rate of the node with different varying number of on-board sensors for a given energy outage probability. Results suggest that for an energy outage probability of 0.1, at New Delhi, a 40 F supercapacitor and a 3 W solar panel can support the operation of Waspmote with 6 on-board toxic gas sensors with a sampling rate of 65 samples per day. Further, we use the proposed models to estimate the practical supercapacitor and solar panel sizes required to ensure sustainability of sensor node operation at different geographical locations with varying sensing rate.

  • Brinkrolf, Christoph
    et al.
    Bielefeld University, Faculty of Technology, Bioinformatics Department, Bielefeld, Germany.
    Henke, Nadja A
    Bielefeld University, Faculty of Biology and CeBiTec, Genetics of Prokaryotes, Bielefeld, Germany.
    Ochel, Lennart
    Linköping University, Department of Computer and Information Science, Software and Systems. Linköping University, Faculty of Science & Engineering. Bielefeld University, Faculty of Technology, Bioinformatics Department, Bielefeld, Germany.
    Pucker, Boas
    Bielefeld University, Faculty of Biology and CeBiTec, Genome Research, Bielefeld, Germany; University of Cambridge, Department of PlantSciences, Evolution and Diversity, Cambridge, UK.
    Kruse, Olaf
    Bielefeld University, Faculty of Biology and CeBiTec, Algae Biotechnology and Bioenergy, Bielefeld, Germany.
    Lutter, Petra
    Bielefeld University, Faculty of Biology and CeBiTec, Proteome and Metabolome Research, Bielefeld, Germany.
    Modeling and Simulating the Aerobic Carbon Metabolism of a Green Microalga Using Petri Nets and New Concepts of VANESA2018In: Journal of integrative bioinformatics, ISSN 1613-4516, Vol. 15, no 3Article in journal (Refereed)
    Abstract [en]

    In this work we present new concepts of VANESA, a tool for modeling and simulation in systems biology. We provide a convenient way to handle mathematical expressions and take physical units into account. Simulation and result management has been improved, and syntax and consistency checks, based on physical units, reduce modeling errors. As a proof of concept, essential components of the aerobic carbon metabolism of the green microalga Chlamydomonas reinhardtii are modeled and simulated. The modeling process is based on xHPN Petri net formalism and simulation is performed with OpenModelica, a powerful environment and compiler for Modelica. VANESA, as well as OpenModelica, is open source, free-of-charge for non-commercial use, and is available at: http://agbi.techfak.uni-bielefeld.de/vanesa.

  • Schildmeijer, Kristina
    et al.
    Department of Health and Caring Sciences Linnaeus University Kalmar Sweden.
    Nilsen, Per
    Linköping University, Department of Medical and Health Sciences, Division of Community Medicine. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Center for Business support and Development.
    Ericsson, Carin
    Region Östergötland, Heart and Medicine Center, Department of Cardiology in Linköping.
    Broström, Anders
    Department of Nursing, School of Health and Welfare Jönköping University Jönköping Sweden.
    Skagerstrom, Janna
    Linköping University, Department of Medical and Health Sciences, Division of Community Medicine. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Regional Board, Research and Development Unit.
    Determinants of patient participation for safer care: A qualitative study of physicians experiences and perceptions2018In: Health science reports, ISSN 2398-8835, Vol. 1, no 10, article id e87Article in journal (Refereed)
    Abstract [en]

    There is a paucity of research on physicians perspectives on involving patients to achieve safer care. This study aims to explore determinants of patient participation for safer care, according to physicians in Swedish health care.

  • Singh, Sukhi
    et al.
    Department of Molecular and Clinical Medicine Institute of Medicine Sahlgrenska Academy University of Gothenburg Gothenburg Sweden.
    Malm, Carl Johan
    Department of Molecular and Clinical Medicine Institute of Medicine Sahlgrenska Academy University of Gothenburg Gothenburg, Sweden.
    Ramström, Sofia
    Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Center for Diagnostics, Department of Clinical Chemistry. School of Medical Sciences Örebro University Örebro,Sweden.
    Hesse, Camilla
    Department of Clinical Chemistry and Transfusion Medicine Institute of Biomedicine Sahlgrenska Academy University of Gothenburg Gothenburg Sweden; Regional Blood Bank Sahlgrenska University Hospital Gothenburg, Sweden.
    Jeppsson, Anders
    Department of Molecular and Clinical Medicine Institute of Medicine Sahlgrenska Academy University of Gothenburg Gothenburg, Sweden.
    Adrenaline enhances in vitro platelet activation and aggregation in blood samples from ticagrelor-treated patients2018In: Research and practice in thrombosis and haemostasis, ISSN 2475-0379, Vol. 2, no 4, p. 718-725Article in journal (Refereed)
    Abstract [en]

    Temporarily improved platelet reactivity may reduce the bleeding in patients on antiplatelet therapy who have ongoing bleeding or who are in need of acute surgery. Adrenaline can bind to adrenergic a2A-receptors on platelets and potentially enhance platelet reactivity.

  • Public defence: 2019-04-26 10:15 TEMCAS, Hus T, Linköping
    Šafarič, Luka
    Linköping University, Department of Thematic Studies, Tema Environmental Change. Linköping University, Faculty of Arts and Sciences.
    Anaerobic Digester Fluid Rheology and Process Efficiency: Interactions of Substrate Composition, Trace Element Availability, and Microbial Activity2019Doctoral thesis, comprehensive summary (Other academic)
    Abstract [en]

    As the anthropogenic greenhouse gas emissions continue imposing stress on our environment, it is becoming increasingly important to identify and implement new renewable technologies. Biogas production through anaerobic digestion has a great potential, since it links waste treatment with extraction of renewable energy, enabling circular bio-economies that are vital for a sustainable future.

    For biogas to have an important role as a renewable energy carrier in society, the scale of its production will need to be increased substantially. New substrates need to be introduced along with raising organic loading rates of the reactors to increase the rate of biogas production. This contributes to challenges in maintaining process stability, thus increasing the risk for process disturbances, including problems that were not commonly encountered before. These difficulties may be particularly pronounced when a broad range of new, largely untested substrates are introduced, leading to an increased heterogeneity of organic material entering the reactors. In the case of currently the most common reactor type; the continuous stirred-tank biogas reactor (CSTBR); such problems may include shifts in rheology (i.e. fluid behaviour) of the anaerobic digester sludge. This may lead to increased energy consumption and decreased digester mixing efficiencies, which in turn may lead to inefficient biogas processes, ultimately decreasing the economic and environmental viability of biogas production. Much is still unknown regarding how rheology shifts happen in biogas reactors, particularly when it comes to what role the substrate plays in rheological dynamics, as compared to the microbial community during varying levels of biogas process stability.

    This thesis elucidates the interactions between substrate type, microbial community and its metabolic activity, and anaerobic sludge rheology. A number of sludge samples from mesophilic and thermophilic CSTBRs digesting a broad range of substrates was analysed for their rheology. The specific effects of individual substrate types on CSTBR sludge rheology and the resulting implications for stirring power requirements and mixing efficiency were investigated. In order to also asses to which extent the microbial metabolism affects rheology at different levels of process disturbance, an experiment with a trace-element-induced inhibition of specific metabolic pathways under mesophilic reactor conditions was performed. This was used to identify the sequence of different interactions that occur in the reactor after the process begins to fail, and to evaluate how these interactions link to changes in digester sludge rheology. Finally, a case study of a disturbed thermophilic anaerobic digestion process was performed, including the monitoring of the response of rheology in relation to process stability, which was modified by changing trace element concentrations. The use of artificial substrate without polymeric compounds in both cases allowed for an evaluation of effects of the microbial community and its metabolic products on rheology without including the effects of complex substrates.

    The results showed that substrate type has a large effect on how different process parameters correlate with fluid behaviour. This was particularly apparent in the case of total solids and total volatile solids, which correlated well with rheological parameters for samples from reactors digesting agricultural waste, sewage sludge, paper mill waste, or food waste, but not for mesophilic co-digesters. Among the different substrates investigated, food waste was generally observed to lead to the highest limit viscosities (i.e. apparent viscosities at high shear rates, where it becomes linear and constant) of the anaerobic sludge, while digestion of paper mill waste and thermophilic co-digestion led to some of the lowest. No fluid type could be clearly coupled to a specific substrate, but it could be observed that increased solids content could generally be associated with more complex, non-Newtonian rheological behaviour. The differences in fluid characteristics between reactors corresponded to large differences in modelled stirring power requirements and mixing efficiency. The results indicated that fluids with high values of rheological parameters, such as the consistency index (K) or yield stress (τ0), would likely require more power or an adapted stirring system to achieve complete mixing. The substrates generally contributed more to the rheology characteristics of the anaerobic sludge than microbial cells on their own. Trace element-induced process disturbance initially led to the inhibition of specific microbial groups among methanogenic archaea or their syntrophic partners, which later escalated to broader inhibition of many microbial groups due to the accumulation of fermentation products. This resulted in microbial cell washout with a corresponding decrease of the contribution of the cells to anaerobic sludge rheology. A recovery of the thermophilic anaerobic digestion process was possible after the supplementation of selenium and tungsten was increased, resulting in increased propionate turnover rates, growing cell densities, and higher viscosity. Major shifts in the methanogenic community were observed, corresponding to the level of process stability. It could be concluded based on these experiments that the specific effect of microbial cells and their activity on sludge rheology were linked to cell density, which corresponded to process stability.

    A conceptual scheme was developed based on the studies in this thesis, defining complex interactions between substrate, microbial metabolism, and anaerobic sludge rheology in biogas processes. The possible causes of rheology shifts are visualised and discussed.

    List of papers
    1. Substrate and operational conditions as regulators of fluid properties in full-scale continuous stirred-tank biogas reactors - implications for rheology-driven power requirements
    Open this publication in new window or tab >>Substrate and operational conditions as regulators of fluid properties in full-scale continuous stirred-tank biogas reactors - implications for rheology-driven power requirements
    Show others...
    2018 (English)In: Water Science and Technology, ISSN 0273-1223, E-ISSN 1996-9732, Vol. 78, no 4, p. 814-826Article in journal (Refereed) Published
    Abstract [en]

    Understanding fluid rheology is important for optimal design and operation of continuous stirred-tank biogas reactors (CSTBRs) and is the basis for power requirement estimates. Conflicting results have been reported regarding the applicability of total solid (TS) and/or total volatile solid (TVS) contents of CSTBR fluids as proxies for rheological properties. Thus, the present study investigates relationships between rheological properties of 12 full-scale CSTBR fluids, their substrate profiles, and major operational conditions, including pH, TS and TVS contents, organic loading rate, hydraulic retention time, and temperature. Rheology-driven power requirements based on various fluid characteristics were evaluated for a general biogas reactor setup. The results revealed a significant correlation only between the rheological fluid properties and TS or TVS contents for sewage sludge digesters and thermophilic co-digesters (CD), but not for mesophilic CD. Furthermore, the calculated power requirements for pumping and mixing, based on the various fluid characteristics of the studied CSTBRs, varied broadly irrespective of TS and TVS contents. Thus, this study shows that the TS and/or TVS contents of digester fluid are not reliable estimators of the rheological properties in CSTBRs digesting substrates other than sewage sludge.

    Place, publisher, year, edition, pages
    IWA PUBLISHING, 2018
    Keywords
    anaerobic digestion; biogas; continuous stirred-tank biogas reactor; power requirement; rheology; viscosity
    National Category
    Other Electrical Engineering, Electronic Engineering, Information Engineering
    Identifiers
    urn:nbn:se:liu:diva-151955 (URN)10.2166/wst.2018.352 (DOI)000445519000010 ()30252659 (PubMedID)
    Note

    Funding Agencies|Innovation Agency (VINNOVA), Sweden [2008-139]; Scandinavian Biogas Fuels AB, Sweden; European Unions Seventh Framework ATBEST Marie-Curie ITN program [316838]; Biogas Research Center; Research Council for Environment, Agricultural Sciences and Spatial Planning (FORMAS), Sweden; Linkoping University; Swedish Energy Agency [35624-2]

    Available from: 2018-10-16 Created: 2018-10-16 Last updated: 2019-04-02
    2. Dynamics of a Perturbed Microbial Community during Thermophilic Anaerobic Digestion of Chemically Defined Soluble Organic Compounds
    Open this publication in new window or tab >>Dynamics of a Perturbed Microbial Community during Thermophilic Anaerobic Digestion of Chemically Defined Soluble Organic Compounds
    Show others...
    2018 (English)In: MICROORGANISMS, ISSN 2076-2607, Vol. 6, no 4, article id 105Article in journal (Refereed) Published
    Abstract [en]

    Knowledge of microbial community dynamics in relation to process perturbations is fundamental to understand and deal with the instability of anaerobic digestion (AD) processes. This study aims to investigate the microbial community structure and function of a thermophilic AD process, fed with a chemically defined substrate, and its association with process performance stability. Next generation amplicon sequencing of 16S ribosomal RNA (rRNA) genes revealed that variations in relative abundances of the predominant bacterial species, Defluviitoga tunisiensis and Anaerobaculum hydrogeniformans, were not linked to the process performance stability, while dynamics of bacterial genera of low abundance, Coprothermobacter and Defluviitoga (other than D. tunisiensis), were associated with microbial community function and process stability. A decrease in the diversity of the archaeal community was observed in conjunction with process recovery and stable performance, implying that the high abundance of specific archaeal group(s) contributed to the stable AD. Dominance of hydrogenotrophic Methanoculleus particularly corresponded to an enhanced microbial acetate and propionate turnover capacity, whereas the prevalence of hydrogenotrophic Methanothermobacter and acetoclastic Methanosaeta was associated with instable AD. Acetate oxidation via syntrophic interactions between Coprothermobacter and Methanoculleus was potentially the main methane-formation pathway during the stable process. We observed that supplementation of Se and W to the medium improved the propionate turnover by the thermophilic consortium. The outcomes of our study provided insights into the community dynamics and trace element requirements in relation to the process performance stability of thermophilic AD.

    Place, publisher, year, edition, pages
    MDPI, 2018
    Keywords
    Thermophilic Anaerobic Digestion; process perturbation; process stability; microbial community dynamics; trace elements
    National Category
    Microbiology
    Identifiers
    urn:nbn:se:liu:diva-154125 (URN)10.3390/microorganisms6040105 (DOI)000455073000010 ()30314333 (PubMedID)
    Note

    Funding Agencies|European Unions Seventh Framework ATBEST Marie-Curie ITN program [316838]; Linkoping University; Swedish Energy Agency [35624-2]; Biogas Research Center

    Available from: 2019-01-29 Created: 2019-01-29 Last updated: 2019-04-02
  • Aksenova, Vasilisa
    et al.
    Institute of Cytology, Russian Academy of Sciences, Tikhoretsky av., 4, St. Petersburg, Russia; Laboratory of Molecular Pharmacology, Saint-Petersburg Technological Institute, 26 Moskovsky Prospect, St. Petersburg, Russia.
    Turoverova, Lidia
    Institute of Cytology, Russian Academy of Sciences, Tikhoretsky av., 4, St. Petersburg, Russia.
    Khotin, Mikhail
    Institute of Cytology, Russian Academy of Sciences, Tikhoretsky av., 4, St. Petersburg, Russia.
    Magnusson, Karl-Eric
    Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Medicine and Health Sciences.
    Tulchinsky, Eugene
    Department of Cancer Studies and Molecular Medicine, University of Leicester, RKCSB, LRI, Leicester, UK.
    Melino, Gerry
    Laboratory of Molecular Pharmacology, Saint-Petersburg Technological Institute, 26 Moskovsky Prospect, St. Petersburg, Russia; MRC Toxicology Unit, Leicester, UK.
    Pinaev, George P
    Institute of Cytology, Russian Academy of Sciences, Tikhoretsky av., 4, St. Petersburg, Russia.
    Barlev, Nickolai
    Institute of Cytology, Russian Academy of Sciences, Tikhoretsky av., 4, St. Petersburg, Russia; Laboratory of Molecular Pharmacology, Saint-Petersburg Technological Institute, 26 Moskovsky Prospect, St. Petersburg, Russia; Department of Biochemistry, University of Leicester, Lancaster Road, Leicester, UK.
    Tentler, Dmitri
    Institute of Cytology, Russian Academy of Sciences, Tikhoretsky av., 4, St. Petersburg, Russia; Laboratory of Molecular Pharmacology, Saint-Petersburg Technological Institute, 26 Moskovsky Prospect, St. Petersburg, Russia.
    Correction: Actin-binding protein alpha-actinin 4 (ACTN4) is a transcriptional co-activator of RelA/p65 sub-unit of NF-kB (vol 4, pg 362, 2013)2018In: OncoTarget, ISSN 1949-2553, E-ISSN 1949-2553, Vol. 9, no 76, p. 34450-34450Article in journal (Other academic)
    Abstract [en]

    [This corrects the article DOI: 10.18632/oncotarget.901.].

  • Jonasson, Hanna
    et al.
    Linköping University, Department of Biomedical Engineering, Division of Biomedical Engineering. Linköping University, Faculty of Science & Engineering. Linköping University, Department of Medical and Health Sciences. Linköping University, Faculty of Medicine and Health Sciences.
    Fredriksson, Ingemar
    Linköping University, Department of Biomedical Engineering, Division of Biomedical Engineering. Linköping University, Faculty of Science & Engineering. Perimed AB, Järfälla, Stockholm, Sweden.
    Bergstrand, Sara
    Linköping University, Department of Medical and Health Sciences, Division of Nursing Science. Linköping University, Faculty of Medicine and Health Sciences.
    Östgren, Carl Johan
    Linköping University, Department of Medical and Health Sciences, Division of Community Medicine. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Primary Care Center, Primary Health Care Center Ödeshög.
    Larsson, Marcus
    Linköping University, Department of Biomedical Engineering, Division of Biomedical Engineering. Linköping University, Faculty of Science & Engineering.
    Strömberg, Tomas
    Linköping University, Department of Biomedical Engineering, Division of Biomedical Engineering. Linköping University, Faculty of Science & Engineering.
    In vivo characterization of light scattering properties of human skin in the 475- to 850-nm wavelength range in a Swedish cohort2018In: Journal of Biomedical Optics, ISSN 1083-3668, E-ISSN 1560-2281, Vol. 23, no 12, article id 121608Article in journal (Refereed)
    Abstract [en]

    We have determined in vivo optical scattering properties of normal human skin in 1734 subjects, mostly with fair skin type, within the Swedish CArdioPulmonary bioImage Study. The measurements were performed with a noninvasive system, integrating spatially resolved diffuse reflectance spectroscopy and laser Doppler flowmetry. Data were analyzed with an inverse Monte Carlo algorithm, accounting for both scattering, geometrical, and absorbing properties of the tissue. The reduced scattering coefficient was found to decrease from 3.16 ± 0.72 to 1.13 ± 0.27 mm-1 (mean ± SD) in the 475- to 850-nm wavelength range. There was a negative correlation between the reduced scattering coefficient and age, and a significant difference between men and women in the reduced scattering coefficient as well as in the fraction of small scattering particles. This large study on tissue scattering with mean values and normal variation can serve as a reference when designing diagnostic techniques or when evaluating the effect of therapeutic optical systems.

  • Stolpstedt, Karl
    Linköping University, Faculty of Arts and Sciences. Linköping University, Department of Management and Engineering, Commercial and Business Law.
    Gårdsförsäljning en möjlighet för Sverige: Farm sales in Sweden- a new possibility2018Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
    Abstract [sv]

    I Sverige har en längre tid debatterats om ett införande av gårdsförsäljning av alkohol- drycker på tillverkningsplatsen. I Sverige tillfaller dock ensamrätten till detaljhandel med alkohol Systembolaget AB. Ska lagstiftningen på denna punkt ändras, måste den samtidigt vara förenlig med EU-rätten på området. Den unionsrättsliga problematiken innefattar den inre marknaden och den fria rörligheten av varor, där ett av ledorden är icke-diskriminering av utländska varor på en fri och öppen marknad. Samtidigt vill vi värna vår svenska rest- riktiva alkoholpolitik. Statliga monopol regleras genom artikel 37 i Fördraget om Europeis- ka Unionens Funktionssätt (FEUF). Artikeln är uteslutande tillämplig på de rättigheter som ges av det direkta utövandet av dess funktionssätt. Från monopolets funktionssätt ska skil- jas alla andra åtgärder, även om de har en påverkan på detta. Dessa regleras genom artikel 34 FEUF, vilken stadgar att varje åtgärd som innebär en kvantitativ importrestriktion eller har en motsvarande verkan ska vara förbjuden. Detta inbegriper varje nationell åtgärd som direkt eller indirekt, faktiskt eller potentiellt, utgör ett hinder för handeln inom unionen. Artikel 36 FEUF ger dock en möjlighet att godta sådana åtgärder om de grundas i ett trän- gande allmänintresse eller andra tvingande hänsyn och är proportionerliga för sina syften. I Finland har gårdsförsäljning funnits sedan 1995 och har sedan årsskiftet utökats till att gäl- la gårdsvin och hantverksöl. I Sverige finns en möjlighet att införa en sådan modell efter finskt snitt. Finland är tillsammans med Sverige de enda medlemsstaterna inom EU med ett detaljhandelsmonopol för alkoholdrycker. Gårdsförsäljning skulle även kunna utgöra en försäljningsform, vilket skulle innebära att åtgärden faller utanför artikel 34. Likaså skulle gårdsförsäljning kunna inlemmas i detaljhandelsmonopolet som ombud till Systembolaget och utgöra filialer till detta. Oaktat vilket alternativ som förespråkas kräver varje sådan åt- gärd en specifik och riktad anpassning till den EU-rätt som berörs.

  • Magnusson, Rebecca
    Linköping University, Department of Management and Engineering, Commercial and Business Law. Linköping University, Faculty of Arts and Sciences.
    Vad är partneravtal?: En analys om termens definition och implikation2019Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
    Abstract [sv]

    Partneravtal är ett växande fenomen i världen, inte enbart i kommersiella sammanhang. Det är en term som har funnits i många år för skilda ändamål och i denna uppsats riktas blicken till partneravtal inom affärsvärlden, som en möjlig associationsform. Partnerskap inleds som en samarbetsform mellan två eller flera företag för att tillsammans uppnå ett gemensamt mål. Det konkreta syftet specificeras i ett partneravtal och utifrån avtalet sker utbyten, såsom kunskap och resurser, mellan parterna i en slags samverkan. I förhållande till andra ”etablerade” samarbetsformer är den främsta skillnaden vid ett partneravtal att sådan avtalsform inte avser att gemensamt bilda ett nytt bolag. 

     

    Problem uppstår dock på grund av att det saknas en juridisk definition av partneravtal. Avsaknaden medför att partneravtal ingås med anledning av olika syften, i varierande former och särskilda krav. Saknaden av juridisk definition medför att partneravtal i dagsläget endast utgör ett avtal utan särskild tillhörighet till en associationsform. Utifrån uppsatsens analys har det dock framkommit att enkla bolag är en associationsform som torde motsvara partneravtalets innebörd, främst avseende de bolagsrekvisit som förutsätts i ett enkelt bolag. Sådana rekvisit kan ses i jämförande utsträckning för partneravtal. Eftersom partneravtal inte torde utgöra en egen associationsform enligt lag, kan i praktiken ett samarbete genom partnerskap leda till att ett enkelt bolag bildas. Likt tanken om att enkla bolag är en ”fri” associationsform, är partnerskapet likaså med anledning av att sådan ”bolagsform” saknar lagreglering och därmed förutbestämda krav. Enkla bolag är dock en lagreglerad associationsform men utgör ett bolagsavtal med viss frihet, eftersom lagbestämmelserna inte är lika omfattande som reglerna för andra associationsformer. Det medför att valfrihet föreligger för enkla bolag, för bildandet och genomförandet, av såsom passivt deltagande och ingen registreringsskyldighet. Med andra ord finns inte ett behov av att partneravtalet ska utgöra en egen associationsform, partneravtalet kan istället definieras som ett bolagsavtal för enkla bolag. 

     

    En jämförelse mellan partneravtal och enkla bolag är förhållandevis inte enkel. Saknaden av definition för partneravtal, i samband med att det är svårt att finna tillämplig innebörd och bolagsrekvisit för enkla bolag, talar för att en tydligare lagstiftning vore önskvärt. Ett förtydligande av enkla bolagets innebörd och bolagsrekvisit skulle minimera tolkningsmöjligheterna och därmed utforma en allmän accepterad definition. Enkla bolag anses utgöra en sorts slask-bolagsform, ändock är enkla bolag en populär associationsform, därför torde det finnas behov av ytterligare utredning av sådant bolagsavtal. En utredning som kanske skulle kunna upptäcka fler samarbetsformer som faller inom associationsformen, enkla bolag.  

  • Savarese, Gianluigi
    et al.
    Cardiology Unit, Department of Medicine , Karolinska Institutet , Stockholm , Sweden.
    Vasko, Peter
    Department of Medicine , Växjö Hospital , Växjö , Sweden.
    Jonsson, Åsa
    Department of Medicine , Ryhov County Hospital , Jönköping , Sweden.
    Edner, Magnus
    Cardiology Unit, Department of Medicine , Karolinska Institutet , Stockholm , Sweden.
    Dahlström, Ulf
    Linköping University, Department of Medical and Health Sciences, Division of Cardiovascular Medicine. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Heart and Medicine Center, Department of Cardiology in Linköping.
    Lund, Lars H
    a Cardiology Unit, Department of Medicine , Karolinska Institutet , Stockholm , Sweden.
    The Swedish Heart Failure Registry: a living, ongoing quality assurance and research in heart failure2019In: Upsala Journal of Medical Sciences, ISSN 0300-9734, E-ISSN 2000-1967, Vol. 124, no 1, p. 65-69Article, review/survey (Refereed)
    Abstract [en]

    Heart failure (HF) represents a global pandemic. Although in HF with reduced ejection fraction (HFrEF) randomized controlled trials have provided effective treatments, prognosis still remains poor, with signals of undertreatment. HF with mid-range EF (HFmrEF) has no evidence-based therapy, and its characterization is ongoing. Trials in HF with preserved EF (HFpEF) have failed to provide any effective treatment, but there are several concerns about their design. Thus, current challenges in the HF field are: 1) optimizing the use of existing treatments in HFrEF; 2) developing and proving efficacy of new treatments, and of new use of existing treatments in HFpEF and HFmrEF. Here we describe how registry-based research can improve knowledge addressing the unmet needs in HF, and in particular we focus on the contribution of the Swedish Heart Failure Registry to this field.

  • Dhanani, Jayesh A
    et al.
    Burns, Trauma and Critical Care Research Centre, The University of Queensland, UQ Centre for Clinical Research, Herston, Brisbane, QLD 4029, Australia; Department of Intensive Care Medicine, Royal Brisbane and Womens Hospital, Brisbane, Australia.
    Parker, Suzanne L
    Burns, Trauma and Critical Care Research Centre, The University of Queensland, UQ Centre for Clinical Research, Herston, Brisbane, QLD 4029, Australia.
    Lipman, Jeffrey
    Burns, Trauma and Critical Care Research Centre, The University of Queensland, UQ Centre for Clinical Research, Herston, Brisbane, QLD 4029, Australia; Department of Intensive Care Medicine, Royal Brisbane and Womens Hospital, Brisbane, Australia; Faculty of Health, Queensland University of Technology, Brisbane, Australia.
    Wallis, Steven C
    Burns, Trauma and Critical Care Research Centre, The University of Queensland, UQ Centre for Clinical Research, Herston, Brisbane, QLD 4029, Australia.
    Cohen, Jeremy
    Burns, Trauma and Critical Care Research Centre, The University of Queensland, UQ Centre for Clinical Research, Herston, Brisbane, QLD 4029, Australia; Department of Intensive Care Medicine, Royal Brisbane and Womens Hospital, Brisbane, Australia.
    Fraser, John
    Critical Care Research Group, The University of Queensland, Brisbane, Australia.
    Barnett, Adrian
    Institute of Health and Biomedical Innovation and School of Public Health and Social Work, Queensland University of Technology, Kelvin Grove, Brisbane, Australia.
    Chew, Michelle S
    Linköping University, Department of Medical and Health Sciences, Division of Drug Research. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Anaesthetics, Operations and Specialty Surgery Center, Department of Anaesthesiology and Intensive Care in Linköping.
    Roberts, Jason A
    Burns, Trauma and Critical Care Research Centre, The University of Queensland, UQ Centre for Clinical Research, Herston, Brisbane, QLD 4029, Australia; Department of Intensive Care Medicine, Royal Brisbane and Womens Hospital, Brisbane, Australia; School of Pharmacy, The University of Queensland, Brisbane, Australia; Department of Pharmacy, Royal Brisbane and Womens Hospital, Brisbane, Australia.
    Recovery rates of combination antibiotic therapy using in vitro microdialysis simulating in vivo conditions2018In: Journal of pharmaceutical analysis, ISSN 2214-0883, Vol. 8, no 6, p. 407-412Article in journal (Refereed)
    Abstract [en]

    Microdialysis is a technique used to measure the unbound antibiotic concentration in the interstitial spaces, the target site of action. In vitro recovery studies are essential to calibrating the microdialysis system for in vivo studies. The effect of a combination of antibiotics on recovery into microdialysate requires investigation. In vitro microdialysis recovery studies were conducted on a combination of vancomycin and tobramycin, in a simulated in vivo model. Comparison was made between recoveries for three different concentrations and three different perfusate flow rates. The overall relative recovery for vancomycin was lower than that of tobramycin. For tobramycin, a concentration of 20µg/mL and flow rate of 1.0µL/min had the best recovery. A concentration of 5.0µg/mL and flow rate of 1.0µL/min yielded maximal recovery for vancomycin. Large molecular size and higher protein binding resulted in lower relative recoveries for vancomycin. Perfusate flow rates and drug concentrations affected the relative recovery when a combination of vancomycin and tobramycin was tested. Low perfusate flow rates were associated with higher recovery rates. For combination antibiotic measurement which includes agents that are highly protein bound, in vitro studies performed prior to in vivo studies may ensure the reliable measurement of unbound concentrations.

  • Dhanani, Jayesh A
    et al.
    Faculty of Medicine, UQ Centre for Clinical Research, The University of Queensland, Brisbane, Australia. jadhanani@hotmail.com; Department of Intensive Care Medicine, Royal Brisbane and Womens Hospital, Brisbane, Australia. jadhanani@hotmail.com; Critical Care Research Group, The University of Queensland, Brisbane, Australia.
    Cohen, Jeremy
    Faculty of Medicine, UQ Centre for Clinical Research, The University of Queensland, Brisbane, Australia; Department of Intensive Care Medicine, Royal Brisbane and Womens Hospital, Brisbane, Australia.
    Parker, Suzanne L
    Faculty of Medicine, UQ Centre for Clinical Research, The University of Queensland, Brisbane, Australia.
    Chan, Hak-Kim
    Advanced Drug Delivery Group, Faculty of Pharmacy, The University of Sydney, Sydney, New South Wales, Australia.
    Tang, Patricia
    Advanced Drug Delivery Group, Faculty of Pharmacy, The University of Sydney, Sydney, New South Wales, Australia.
    Ahern, Benjamin J
    Faculty of Science, School of Veterinary Science, The University of Queensland, Gatton, Australia.
    Khan, Adeel
    Faculty of Science, School of Veterinary Science, The University of Queensland, Gatton, Australia.
    Bhatt, Manoj
    Department of Nuclear Medicine and Specialised PET Services Queensland, Royal Brisbane and Womens Hospital, Herston, Queensland, Australia; School of Medicine, Faculty of Health Sciences, University of Queensland, St Lucia, Queensland, Australia.
    Goodman, Steven
    Department of Nuclear Medicine and Specialised PET Services Queensland, Royal Brisbane and Womens Hospital, Herston, Queensland, Australia.
    Diab, Sara
    Critical Care Research Group, The University of Queensland, Brisbane, Australia.
    Chaudhary, Jivesh
    Critical Care Research Group, The University of Queensland, Brisbane, Australia.
    Lipman, Jeffrey
    Faculty of Medicine, UQ Centre for Clinical Research, The University of Queensland, Brisbane, Australia; Department of Intensive Care Medicine, Royal Brisbane and Womens Hospital, Brisbane, Australia; Faculty of Health, Queensland University of Technology, Brisbane, Australia.
    Wallis, Steven C
    Faculty of Medicine, UQ Centre for Clinical Research, The University of Queensland, Brisbane, Australia.
    Barnett, Adrian
    Institute of Health and Biomedical Innovation and School of Public Health and Social Work, Queensland University of Technology, Kelvin Grove, Brisbane, Australia.
    Chew, Michelle S
    Linköping University, Department of Medical and Health Sciences, Division of Drug Research. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Anaesthetics, Operations and Specialty Surgery Center, Department of Anaesthesiology and Intensive Care in Linköping.
    Fraser, John F
    Critical Care Research Group, The University of Queensland, Brisbane, Australia.
    Roberts, Jason A
    Faculty of Medicine, UQ Centre for Clinical Research, The University of Queensland, Brisbane, Australia; Department of Intensive Care Medicine, Royal Brisbane and Womens Hospital, Brisbane, Australia; Centre for Translational Anti-infective Pharmacodynamics, School of Pharmacy, The University of Queensland, Brisbane, Australia; Department of Pharmacy, Royal Brisbane and Womens Hospital, Brisbane, Australia.
    A research pathway for the study of the delivery and disposition of nebulised antibiotics: an incremental approach from in vitro to large animal models2018In: Intensive Care Medicine Experimental, ISSN 1646-2335, E-ISSN 2197-425X, Vol. 6, no 1, article id 17Article in journal (Refereed)
    Abstract [en]

    Nebulised antibiotics are frequently used for the prevention or treatment of ventilator-associated pneumonia. Many factors may influence pulmonary drug concentrations with inaccurate dosing schedules potentially leading to therapeutic failure and/or the emergence of antibiotic resistance. We describe a research pathway for studying the pharmacokinetics of a nebulised antibiotic during mechanical ventilation using in vitro methods and ovine models, using tobramycin as the study antibiotic.

  • Alftberg, Åsa
    et al.
    Department of Social Work, Faculty of Health and Society, Malmö University, Malmö, Sweden.
    Ahlström, Gerd
    Department of Health Sciences, Faculty of Medicine, Lund University, Sweden.
    Nilsen, Per
    Linköping University, Department of Medical and Health Sciences, Division of Community Medicine. Linköping University, Faculty of Medicine and Health Sciences.
    Behm, Lina
    Department of Health Sciences, Faculty of Medicine, Lund University, Sweden.
    Sandgren, Anna
    Center for Collaborative Palliative Care, Department of Health and Caring Sciences, Faculty of Health and Life Sciences, Linnaeus University, Växjö, Sweden.
    Benzein, Eva
    Center for Collaborative Palliative Care, Department of Health and Caring Sciences, Faculty of Health and Life Sciences, Linnaeus University,Växjö, Sweden.
    Wallerstedt, Birgitta
    Center for Collaborative Palliative Care, Department of Health and Caring Sciences, Faculty of Health and Life Sciences, Linnaeus University, Växjö, Sweden.
    Rasmussen, Birgit H.
    Department of Health Sciences, Faculty of Medicine, Lund University, P.O. Box 157, SE-221 00 Lund, Sweden. Birgit.Rasmussen@med.lu.se; The Institute for Palliative Care, Region Skane and Lund University, Lund, Sweden.
    Conversations about Death and Dying with Older People: An Ethnographic Study in Nursing Homes2018In: Healthcare, E-ISSN 2227-9032, Vol. 6, no 2, article id 63Article in journal (Refereed)
    Abstract [en]

    Nursing homes are often places where older persons amp;ldquo;come to die.amp;rdquo; Despite this, death and dying are seldom articulated or talked about. The aim of this study was to explore assistant nursesamp;rsquo; experiences of conversations about death and dying with nursing home residents. This study is part of an implementation project through a knowledge-based educational intervention based on palliative care principles. An ethnographic study design was applied in seven nursing homes, where eight assistant nurses were interviewed and followed in their daily assignments through participant observations. The assistant nurses stated that they had the knowledge and tools to conduct such conversations, even though they lacked the time and felt that emotional strain could be a hinder for conversations about death and dying. The assistant nurses used the strategies of distracting, comforting, and disregarding either when they perceived that residentsamp;rsquo; reflections on death and dying were part of their illness and disease or when there was a lack of alignment between the residentsamp;rsquo; contemplations and the concept of dying well. They indicated that ambivalence and ambiguity toward conversations about death and dying should be taken into consideration in future implementations of knowledge-based palliative care that take place in nursing homes after this project is finalized.

  • Garrido, Mario
    Linköping University, Department of Electrical Engineering, Computer Engineering. Linköping University, Faculty of Science & Engineering.
    Multiplexer and Memory-Efficient Circuits for Parallel Bit Reversal2019In: IEEE Transactions on Circuits and Systems - II - Express Briefs, ISSN 1549-7747, E-ISSN 1558-3791, Vol. 66, no 4, p. 657-661Article in journal (Refereed)
    Abstract [en]

    This brief presents novel circuits for calculating the bit reversal on parallel data. The circuits consist of delays/memories and multiplexers, and have the advantage that they requires the minimum number of multiplexers among circuits for parallel bit reversal so far, as well as a small total memory.

  • Garrido, Mario
    et al.
    Linköping University, Department of Electrical Engineering, Computer Engineering. Linköping University, Faculty of Science & Engineering.
    Möller, K.
    University of Kassel, Kassel, Germany.
    Kumm, M.
    University of Kassel, Kassel, Germany.
    World’s Fastest FFT Architectures: Breaking the Barrier of 100 GS/s2019In: IEEE Transactions on Circuits and Systems Part 1: Regular Papers, ISSN 1549-8328, E-ISSN 1558-0806, Vol. 66, no 4, p. 1507-1516Article in journal (Refereed)
    Abstract [en]

    This paper presents the fastest fast Fourier transform (FFT) hardware architectures so far. The architectures are based on a fully parallel implementation of the FFT algorithm. In order to obtain the highest throughput while keeping the resource utilization low, we base our design on making use of advanced shift-and-add techniques to implement the rotators and on selecting the most suitable FFT algorithms for these architectures. Apart from high throughput and resource efficiency, we also guarantee high accuracy in the proposed architectures. For the implementation, we have developed an automatic tool that generates the architectures as a function of the FFT size, input word length and accuracy of the rotations. We provide experimental results covering various FFT sizes, FFT algorithms, and field-programmable gate array boards. These results show that it is possible to break the barrier of 100 GS/s for FFT calculation.

  • Adolfsson, Lars
    Linköping University, Department of Clinical and Experimental Medicine, Division of Surgery, Orthopedics and Oncology. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Center for Surgery, Orthopaedics and Cancer Treatment, Department of Orthopaedics in Linköping.
    Post-traumatic stiff elbow2018In: EFORT open reviews, ISSN 2058-5241, Vol. 3, no 5, p. 210-216Article, review/survey (Refereed)
    Abstract [en]

    Post-traumatic and post-operative stiffness of the elbow joint is relatively common and may in pronounced cases markedly interfere with normal upper extremity function.Soft-tissue contractures and heterotopic bone formation are two major causes of limited movement.Extensive recent research has elucidated many of the pathways contributing to these conditions, but the exact mechanisms are still unknown.In the early phase of soft-tissue contractures conservative treatment may be valuable, but in longstanding cases operative treatment is often necessary.Several different options are available depending on the severity of the condition and the underlying offending structures. Surgical treatment may allow significant gains in movement but rarely complete restoration, and complications are not uncommon.The following presentation reviews the recent literature on pathomechanisms and treatment alternatives. Cite this article: EFORT Open Rev 2018;3 DOI: 10.1302/2058-5241.3.170062.

  • Birgitta Gunnarsson, A.
    et al.
    Department of Research and Development, Region Kronoberg, PO Box 1223, SE-351 12, Växjö, Sweden. birgitta.gunnarsson@kronoberg.se; Department of Clinical Neuroscience and Rehabilitation, University of Gothenburg, Gothenburg, Sweden. birgitta.gunnarsson@kronoberg.se.
    Wagman, Petra
    School of Health and Welfare, Department of Rehabilitation, Jönköping University, Jönköping, Sweden.
    Hedin, Katarina
    Linköping University, Department of Medical and Health Sciences, Division of Community Medicine. Linköping University, Faculty of Medicine and Health Sciences. Department of Clinical Sciences in Malmö, Family Medicine Lund University, Lund, Sweden.
    Håkansson, Carita
    Division of Occupational and Environmental Medicine, Lund University, Lund, Sweden.
    Treatment of depression and/or anxiety - outcomes of a randomised controlled trial of the tree theme method® versus regular occupational therapy2018In: BMC Psychology, E-ISSN 2050-7283, Vol. 6, no 1, article id 25Article in journal (Refereed)
    Abstract [en]

    Depression and anxiety disorders are a major concern in western countries, and because these often have a negative affect on everyday life interventions based on activities in everyday life are needed. The Tree Theme Method® (TTM) is a client-centred occupational therapy intervention designed to increase the ability to cope with, and to enhance satisfaction with, everyday life, both at home and at work. The aim of this study was to compare the short term outcomes of the TTM intervention with regular occupational therapy treatment for people with depression and/or anxiety disorders.

  • Gopinath, Madhumala
    et al.
    Department of Allied Health Sciences, Chettinad Hospital and Research Institute (CHRI), Chettinad Academy of Research and Education (CARE), Kelambakkam, Chennai-603103, India.
    Di Liddo, Rosa
    Department of Pharmacology and Pharmaceutical Sciences, University of Padova, Padova, Italy.
    Marotta, Francesco
    ReGenera RandD International for Aging Intervention, Milano-Beijing, Italy-China, VCC Preventive Medical Promotion Foundation, Beijing, China.
    Murugesan, Ramachandran
    Department of Allied Health Sciences, Chettinad Hospital and Research Institute (CHRI), Chettinad Academy of Research and Education (CARE), Kelambakkam, Chennai-603103, India.
    Banerjee, Antara
    Department of Allied Health Sciences, Chettinad Hospital and Research Institute (CHRI), Chettinad Academy of Research and Education (CARE), Kelambakkam, Chennai-603103, India.
    Sriramulu, Sushmitha
    Department of Allied Health Sciences, Chettinad Hospital and Research Institute (CHRI), Chettinad Academy of Research and Education (CARE), Kelambakkam, Chennai-603103, India.
    Jothimani, Ganesan
    Department of Allied Health Sciences, Chettinad Hospital and Research Institute (CHRI), Chettinad Academy of Research and Education (CARE), Kelambakkam, Chennai-603103, India.
    Subramaniam, Vimala Devi
    Department of Allied Health Sciences, Chettinad Hospital and Research Institute (CHRI), Chettinad Academy of Research and Education (CARE), Kelambakkam, Chennai-603103, India.
    Narasimhan, Srinivasan
    Department of Allied Health Sciences, Chettinad Hospital and Research Institute (CHRI), Chettinad Academy of Research and Education (CARE), Kelambakkam, Chennai-603103, India.
    Priya K, Swarna
    Department of Gynecology and Pediatrics, Chettinad Hospital and Research Institute (CHRI), Chettinad Academy of Research and Education (CARE), Kelambakkam, Chennai-603103, India.
    Sun, Xiao-Feng
    Linköping University, Department of Clinical and Experimental Medicine, Division of Surgery, Orthopedics and Oncology. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Center for Surgery, Orthopaedics and Cancer Treatment, Department of Oncology.
    Pathak, Surajit
    Department of Allied Health Sciences, Chettinad Hospital and Research Institute (CHRI), Chettinad Academy of Research and Education (CARE), Kelambakkam, Chennai-603103, India.
    Role of Hippo Pathway Effector Tafazzin Protein in Maintaining Stemness of Umbilical Cord-Derived Mesenchymal Stem Cells (UC-MSC)2018In: International Journal of Hematology-Oncology and Stem Cell Research, ISSN 2008-3009, E-ISSN 2008-2207, Vol. 12, no 2, p. 153-165Article, review/survey (Refereed)
    Abstract [en]

    Tafazzin (TAZ) protein has been upregulated in various types of human cancers, although the basis for elevation is uncertain, it has been made definite that the effect of mutation in the hippo pathway, particularly when it is switched off, considerably activates tafazzin transcriptionally and thus this results in tissue or tumor overgrowth. Recent perceptions into the activity of tafazzin, have ascribed to it, a role as stem cell factor in mouse mesenchymal and as well as in neural stem cells. Being a downstream molecule in Hippo signalling, phosphorylation or dephosphorylation of tafazzin gene regulates its transcriptional activity and the stemness of mesenchymal stem cells. Commonly, extracellular matrix controls the stem cell fate commitment and perhaps tafazzin controls stemness through altering the extra cellular matrix. Extracellular matrix is generally made up of prime proteoglycans and the fate stabilization of the resulting lineages is surveilled by engineering these glycans. Tafazzin degradation and addition of proteoglycans affect physical attributes of the extracellular matrix that drives cell differentiation into various lineages. Thus, tafazzin along with major glycans present in the extracellular matrix is involved in imparting stemness. However, there are incoherent molecular events, wherein both tafazzin and the extracellular matrix components, together either activate or inhibit differentiation of stem cells. This review discusses about the role of tafazzin oncoprotein as a stemness factor.

  • Hemmingsson, Jens
    et al.
    Department of Radiation Physics, The Sahlgrenska Academy, Sahlgrenska University Hospital, Gothenburg, Sweden.
    Högberg, Jonas
    Region Östergötland, Center for Diagnostics, Medical radiation physics.
    Mölne, Johan
    Department of Pathology, The Sahlgrenska Academy, Sahlgrenska University Hospital, Gothenburg, Sweden.
    Svensson, Johanna
    Department of Oncology, The Sahlgrenska Academy, Sahlgrenska University Hospital, Gothenburg, Sweden.
    Gjertsson, Peter
    Department of Clinical Physiology, The Sahlgrenska Academy, Sahlgrenska University Hospital, Gothenburg, Sweden.
    Rizell, Magnus
    Department of Surgery, The Sahlgrenska Academy, Sahlgrenska University Hospital, Gothenburg, Sweden.
    Henrikson, Olof
    Department of Radiology, The Sahlgrenska Academy, Sahlgrenska University Hospital, Gothenburg, Sweden.
    Bernhardt, Peter
    Department of Radiation Physics, The Sahlgrenska Academy, Sahlgrenska University Hospital, Gothenburg, Sweden.
    Autoradiography and biopsy measurements of a resected hepatocellular carcinoma treated with 90 yttrium radioembolization demonstrate large absorbed dose heterogeneities2018In: Advances in radiation oncology, ISSN 2452-1094, Vol. 3, no 3, p. 439-446Article in journal (Refereed)
    Abstract [en]

    Radioembolization is an alternative palliative treatment for hepatocellular carcinoma. Here, we examine the uptake differences between tumor tissue phenotypes and present a cross-section of the absorbed dose throughout a liver tissue specimen.

  • Paul, Biplab
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Thin Film Physics. Linköping University, Faculty of Science & Engineering.
    Björk, Emma M.
    Linköping University, Department of Physics, Chemistry and Biology, Nanostructured Materials. Linköping University, Faculty of Science & Engineering.
    Kumar, Aparabal
    Materials Science Centre, Indian Institute of Technology, Kharagpur 721302, India.
    Lu, Jun
    Linköping University, Department of Physics, Chemistry and Biology, Thin Film Physics. Linköping University, Faculty of Science & Engineering.
    Eklund, Per
    Linköping University, Department of Physics, Chemistry and Biology, Thin Film Physics. Linköping University, Faculty of Science & Engineering.
    Nanoporous Ca3Co4O9 Thin Films for Transferable Thermoelectrics2018In: ACS applied energy materials, ISSN 2574-0962, Vol. 1, no 5, p. 2261-2268Article in journal (Refereed)
    Abstract [en]

    The development of high-performance and transferable thin-film thermoelectric materials is important for low-power applications, e.g., to power wearable electronics, and for on-chip cooling. Nanoporous films offer an opportunity to improve thermoelectric performance by selectively scattering phonons without affecting electronic transport. Here, we report the growth of nanoporous Ca3Co4O9 thin films by a sequential sputtering-annealing method. Ca3Co4O9 is promising for its high Seebeck coefficient and good electrical conductivity and important for its nontoxicity, low cost, and abundance of its constituent raw materials. To grow nanoporous films, multilayered CaO/CoO films were deposited on sapphire and mica substrates by rf-magnetron reactive sputtering from elemental Ca and Co targets, followed by annealing at 700 C to form the final phase of Ca3Co4O9. This phase transformation is accompanied by a volume contraction causing formation of nanopores in the film. The thermoelectric propoperties of the nanoporous Ca3Co4O9 films can be altered by controlling the porosity. The lowest electrical resistivity is ~7 mO cm, yielding a power factor of 2.32 × 10-4 Wm-1K-2 near room temperature. Furthermore, the films are transferable from the primary mica substrates to other arbitrary polymer platforms by simple dry transfer, which opens an opportunity of low-temperature use these materials.

  • Toh, Yi-Chin
    et al.
    Department of Biomedical Engineering, 4 Engineering Drive, National University of Singapore, Singapore 117853, Singapore. biety@nus.edu.sg; Institute of Bioengineering and Nanotechnology, A*STAR, The Nanos, #04-01, 31 Biopolis Way, Singapore 138669, Singapore.
    Raja, Anju
    Institute of Bioengineering and Nanotechnology, A*STAR, The Nanos, #04-01, 31 Biopolis Way, Singapore 138669, Singapore. anju.mythreyi.raja@ihis.com.sg; Integrated Health Information Systems (IHiS), 6 Serangoon North Avenue 5, Singapore 554910, Singapore.
    Yu, Hanry
    Institute of Bioengineering and Nanotechnology, A*STAR, The Nanos, #04-01, 31 Biopolis Way, Singapore 138669, Singapore. medyuh@nus.edu.sg; Department of Physiology, Yong Loo Lin School of Medicine, MD9-04-11, 2 Medical Drive, Singapore 117597, Singapore. medyuh@nus.edu.sg; Mechanobiology Institute, National University of Singapore, T-Lab, #05-01, 5A Engineering Drive 1, Singapore 117411, Singapore. medyuh@nus.edu.sg; Singapore-MIT Alliance for Research and Technology, 1 CREATE Way, #10-01 CREATE Tower, Singapore 138602, Singapore. medyuh@nus.edu.sg; NUS Graduate Programme in Bioengineering, NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore 117597, Singapore. medyuh@nus.edu.sg; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. medyuh@nus.edu.sg; Gastroenterology Department, Southern Medical University, Guangzhou 510515, China.
    van Noort, Danny
    Linköping University, Department of Physics, Chemistry and Biology, Biotechnology. Linköping University, Faculty of Science & Engineering. Department of New Biology, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu 42988, Korea.
    A 3D Microfluidic Model to Recapitulate Cancer Cell Migration and Invasion2018In: Bioengineering (Basel, Switzerland), ISSN 2306-5354, Vol. 5, no 2Article in journal (Refereed)
    Abstract [en]

    We have developed a microfluidic-based culture chip to simulate cancer cell migration and invasion across the basement membrane. In this microfluidic chip, a 3D microenvironment is engineered to culture metastatic breast cancer cells (MX1) in a 3D tumor model. A chemo-attractant was incorporated to stimulate motility across the membrane. We validated the usefulness of the chip by tracking the motilities of the cancer cells in the system, showing them to be migrating or invading (akin to metastasis). It is shown that our system can monitor cell migration in real time, as compare to Boyden chambers, for example. Thus, the chip will be of interest to the drug-screening community as it can potentially be used to monitor the behavior of cancer cell motility, and, therefore, metastasis, in the presence of anti-cancer drugs.

  • Mak, Wing Cheung
    et al.
    Linköping University, Department of Physics, Chemistry and Biology, Sensor and Actuator Systems. Linköping University, Faculty of Science & Engineering.
    Olesen, Kim
    Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Medicine and Health Sciences.
    Sivlér, Petter
    Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Medicine and Health Sciences.
    Lee, Chyan-Jang
    Linköping University, Department of Clinical and Experimental Medicine. Linköping University, Faculty of Medicine and Health Sciences.
    Moreno-Jimenez, Ines
    Linköping University, Department of Clinical and Experimental Medicine. Linköping University, Faculty of Medicine and Health Sciences. Bone & Joint Research Group, Stem Cells & Regeneration Institute of Developmental Sciences, Southampton General Hospital, UK.
    Edin, Joel
    Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Science & Engineering.
    Courtman, D.
    Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, Canada.
    Skog, Mårten
    Linköping University, Department of Clinical and Experimental Medicine. Linköping University, Faculty of Medicine and Health Sciences.
    Griffith, May
    Linköping University, Department of Clinical and Experimental Medicine, Division of Cell Biology. Linköping University, Faculty of Medicine and Health Sciences.
    Correction: W.C. Mak, et al. Controlled Delivery of Human Cells by Temperature Responsive Microcapsules. J. Funct. Biomater. 2015, 6, 439-4532018In: Journal of Functional Biomaterials, ISSN 2079-4983, E-ISSN 2079-4983, Vol. 9, no 2, article id 26Article in journal (Other academic)
  • Sjögren, Hans
    Linköping University, Department of Management and Engineering, Economics. Linköping University, Faculty of Arts and Sciences.
    Smorgasbord: varumärke och flygförsäkring - när Skandinavien flög ut över världen2019 (ed. 1)Book (Other academic)
    Abstract [sv]

    Denna studie är mångvetenskaplig och spänner från kulturvetenskap över till ekonomisk historia och företagsekonomi. Den utgör en fristående fortsättning på en tidigare bok om flygmarknaden i Skandinavien: Högtryck. SAS och omvandlingen, utgiven 2015. Titeln är dubbelbottnad. Dels användes smörgåsbordet, eller Smorgasbord, i marknadsföringen av Scandinavia som varumärke, dels har boken ett upplägg som påminner om ett smörgåsbord, med en presentation av mer eller mindre fristående delar som berör flygmarknaden. Boken är uppdelad i två självständiga avsnitt, Meningsskapande och varumärke respektive Intraprenörskap och strukturkapital, med tre kapitel inom varje avsnitt. Det första avsnittet behandlar vilka föreställningar som finns om Skandinavien och hur det skandinaviska kom att konstrueras och fysiskt gestaltas från 1950-talet och framåt. I det andra avsnittet ligger fokus på behovet av trygghet och försäkringar på den civila flygmarknaden, där en svensk organisation utvecklade världsledande system för att prissätta och försäkra risker, på flygmarknaden och senare även på marknaden för rymdförsäkringar (satellituppskjutningar). Bokens kapitel har således behovet av att skapa förtroende på flygmarknaden som minsta gemensamma nämnare. Dessutom löper entreprenörskap och intraprenöriella processer som en röd tråd genom hela boken, skildrat genom två organisationers historia – flygbolaget SAS och försäk-ringsbolaget Skandia – från 1950-talet fram till slutet av 2010-talet. Det är bara att smaka på utifrån eget intresse.

  • Tribonias, George
    et al.
    Region Östergötland, Heart and Medicine Center, Department of Gastroentorology.
    Daferera, Niki
    Linköping University, Department of Clinical and Experimental Medicine, Division of Neuro and Inflammation Science. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Heart and Medicine Center, Department of Gastroentorology.
    Manola, Margarita-Eleni
    Region Östergötland, Heart and Medicine Center, Department of Gastroentorology.
    Svernlöv, Rikard
    Region Östergötland, Heart and Medicine Center, Department of Gastroentorology.
    Ignatova, Simone
    Linköping University, Department of Clinical and Experimental Medicine, Divison of Neurobiology. Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Center for Diagnostics, Clinical pathology.
    Hjortswang, Henrik
    Linköping University, Faculty of Medicine and Health Sciences. Region Östergötland, Heart and Medicine Center, Department of Gastroentorology. Linköping University, Department of Clinical and Experimental Medicine, Division of Neuro and Inflammation Science.
    How familiar are we with decision-making concerning the treatment of perforation after endoscopic mucosal resection (EMR) in the colon? A case report2018In: Endoscopy International Open, ISSN 2196-9736, E-ISSN 2196-9736, Vol. 6, no 3, p. E308-E312Article in journal (Refereed)
    Abstract [en]

    Background and study aims We describe a case of perforation after colonic endoscopic mucosal resection (EMR) that was treated conservatively. We would like to highlight the importance of decision-making mainly based on the endoscopists point of view in combination with the surgical consultation. Although the radiological imaging is always needed, it cannot solely lead to a decision for operation. Intraperitoneal gas in computed tomography is not always associated with a hole in the endoscopic field and could be possibly explained from a "balloon" phenomenon. The amount of extraluminal air after an EMR does not correlate reciprocally with patients pain after the procedure. Even though perforation is a radiological diagnosis and endoscopists should be aware of the common post-EMR radiological findings, the surgical examination is mandatory and should be coupled with the endoscopic opinion in order to guide appropriately the treatment in patients with acute pain.