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Shabo, Ivan
Publikasjoner (10 av 16) Visa alla publikasjoner
Aljabery, F., Olsson, H., Gimm, O., Jahnson, S. & Shabo, I. (2018). M2-macrophage infiltration and macrophage traits of tumor cells in urinary bladder cancer. Urologic Oncology, 36(4), Article ID 159.e19.
Åpne denne publikasjonen i ny fane eller vindu >>M2-macrophage infiltration and macrophage traits of tumor cells in urinary bladder cancer
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2018 (engelsk)Inngår i: Urologic Oncology, ISSN 1078-1439, E-ISSN 1873-2496, Vol. 36, nr 4, artikkel-id 159.e19Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Background

Tumor-associated macrophages (TAMs) constitute a subset of nonneoplastic cells in tumor stroma and influence cancer progression in solid tumors. The clinical significance of TAMs in urinary bladder cancer(UBC) is controversial.

Methods

We prospectively studied 103 patients with stage pT1–T4 UBC treated with cystectomy and pelvic lymph node dissection. Tumor sections were immunostained with M2-specific macrophage marker CD163 and proliferation marker Ki-67. The expression of these markers in cancer cells as well as macrophage infiltration (MI) in tumor stroma was analyzed in relation to clinical data and outcome.

Results

The mean rate of CD163 and Ki-67 expressed by cancer cells were 35% and 78%, respectively. With borderline significance, MI was associated with lower rate of lymph node metastasis (P = 0.06). CD163 expression in cancer cells was proportional to MI (P<0.014). Patients with CD163-positive tumors and strong MI had significantly longer cancer-specific survival (CSS) (76 months), compared to patient with CD163-positive tumors and weak MI (28 months) (P = 0.02).

Conclusions

M2-specific MI tends to be inversely correlated with LN metastasis and improved CSS in UBC. MI might have protective impact in CD163-positive tumors. Expression of CD163 in cancer cells is significantly correlated with MI and might have a tumor promoting impact.

sted, utgiver, år, opplag, sider
Elsevier, 2018
Emneord
Bladder cancer; Tumor-associated macrophages; Lymph node metastasis; Ki-67; CD163; Macrophage traits in tumor cells
HSV kategori
Identifikatorer
urn:nbn:se:liu:diva-147811 (URN)10.1016/j.urolonc.2017.11.020 (DOI)000429902600013 ()29288002 (PubMedID)2-s2.0-85039047709 (Scopus ID)
Merknad

Funding Agencies|FoU research grant from the County Council of Ostergotland, Linkoping, Sweden; ALF research grant from the County Council of Ostergotland, Linkoping, Sweden

Tilgjengelig fra: 2018-05-17 Laget: 2018-05-17 Sist oppdatert: 2018-05-24bibliografisk kontrollert
Haj-Hosseini, N., Petersson, P., Gimm, O. & Shabo, I. (2016). Optical Coherence Tomography for Pathological Analysis of Thyroid. In: : . Paper presented at SPIE Photonics West, 16 - 18-February 2016 San Francisco, California, United States. San Francisco
Åpne denne publikasjonen i ny fane eller vindu >>Optical Coherence Tomography for Pathological Analysis of Thyroid
2016 (engelsk)Konferansepaper, Poster (with or without abstract) (Fagfellevurdert)
sted, utgiver, år, opplag, sider
San Francisco: , 2016
HSV kategori
Identifikatorer
urn:nbn:se:liu:diva-126690 (URN)
Konferanse
SPIE Photonics West, 16 - 18-February 2016 San Francisco, California, United States
Forskningsfinansiär
Medical Research Council of Southeast Sweden (FORSS)Linköpings universitet
Tilgjengelig fra: 2016-04-01 Laget: 2016-04-01 Sist oppdatert: 2018-04-25
Shabo, I., Midtbö, K. M., Andersson, H., Åkerlund, E., Olsson, H., Wegman, P., . . . Lindström, A. (2015). Macrophage traits in cancer cells are induced by macrophage-cancer cell fusion and cannot be explained by cellular interaction. BMC Cancer, 15(1), 922
Åpne denne publikasjonen i ny fane eller vindu >>Macrophage traits in cancer cells are induced by macrophage-cancer cell fusion and cannot be explained by cellular interaction
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2015 (engelsk)Inngår i: BMC Cancer, ISSN 1471-2407, E-ISSN 1471-2407, Vol. 15, nr 1, s. 922-Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Background: Cell fusion is a natural process in normal development and tissue regeneration. Fusion between cancer cells and macrophages generates metastatic hybrids with genetic and phenotypic characteristics from both maternal cells. However, there are no clinical markers for detecting cell fusion in clinical context. Macrophage-specific antigen CD163 expression in tumor cells is reported in breast and colorectal cancers and proposed being caused by macrophages-cancer cell fusion in tumor stroma. The purpose of this study is to examine the cell fusion process as a biological explanation for macrophage phenotype in breast. Methods: Monocytes, harvested from male blood donor, were activated to M2 macrophages and co-cultured in ThinCert transwell system with GFP-labeled MCF-7 cancer cells. MCF7/macrophage hybrids were generated by spontaneous cell fusion, isolated by fluorescence-activated cell sorting and confirmed by fluorescence microscopy, short tandem repeats analysis and flow cytometry. CD163 expression was evaluated in breast tumor samples material from 127 women by immunohistochemistry. Results: MCF-7/macrophage hybrids were generated spontaneously at average rate of 2 % and showed phenotypic and genetic traits from both maternal cells. CD163 expression in MCF-7 cells could not be induced by paracrine interaction with M2-activated macrophages. CD163 positive cancer cells in tumor sections grew in clonal collection and a cutoff point greater than25 % of positive cancer cells was significantly correlated to disease free and overall survival. Conclusions: In conclusion, macrophage traits in breast cancer might be caused by cell fusion rather than explained by paracrine cellular interaction. These data provide new insights into the role of cell fusion in breast cancer and contributes to the development of clinical markers to identify cell fusion.

sted, utgiver, år, opplag, sider
BIOMED CENTRAL LTD, 2015
Emneord
Cell fusion; Macrophages; Paracrine cellular interaction; Tumor markers
HSV kategori
Identifikatorer
urn:nbn:se:liu:diva-123328 (URN)10.1186/s12885-015-1935-0 (DOI)000365276000001 ()26585897 (PubMedID)
Merknad

Funding Agencies|County Council of Ostergotland (Sweden); National Organization of Breast Cancer Associations (Sweden); Bengt Ihre Foundation (Swedish Surgical Society)

Tilgjengelig fra: 2015-12-14 Laget: 2015-12-11 Sist oppdatert: 2018-01-10
Haj-Hosseini, N., Stepp, H., Markwardt, N., Gimm, O. & Shabo, I. (2015). Optical biopsy during thyroid and parathyroid surgery. In: : . Paper presented at European Conferences on Biomedical Optics, Munich, Germany, 21-25 June. Munich: SPIE - International Society for Optical Engineering
Åpne denne publikasjonen i ny fane eller vindu >>Optical biopsy during thyroid and parathyroid surgery
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2015 (engelsk)Konferansepaper, Poster (with or without abstract) (Fagfellevurdert)
sted, utgiver, år, opplag, sider
Munich: SPIE - International Society for Optical Engineering, 2015
Emneord
optical coherence tomography, autofluorescene, thyroid, parathyroid
HSV kategori
Identifikatorer
urn:nbn:se:liu:diva-118520 (URN)
Konferanse
European Conferences on Biomedical Optics, Munich, Germany, 21-25 June
Forskningsfinansiär
Medical Research Council of Southeast Sweden (FORSS)
Tilgjengelig fra: 2015-05-29 Laget: 2015-05-29 Sist oppdatert: 2018-04-25bibliografisk kontrollert
Aljabery, F., Lindblom, G., Skoog, S., Shabo, I., Olsson, H., Rosell, J. & Jahnson, S. (2015). PET/CT versus conventional CT for detection of lymph node metastases in patients with locally advanced bladder cancer.. BMC urology, 15(1), 87
Åpne denne publikasjonen i ny fane eller vindu >>PET/CT versus conventional CT for detection of lymph node metastases in patients with locally advanced bladder cancer.
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2015 (engelsk)Inngår i: BMC urology, ISSN 1471-2490, Vol. 15, nr 1, s. 87-Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

BACKGROUND: We studied patients treated with radical cystectomy for locally advanced bladder cancer to compare the results of both preoperative positron emission tomography/computed tomography (PET/CT) and conventional CT with the findings of postoperative histopathological evaluation of lymph nodes.

METHODS: Patients who had bladder cancer and were candidates for cystectomy underwent preoperative PET/CT using 18-fluorodeoxyglucose (FDG) and conventional CT. The results regarding lymph node involvement were independently evaluated by two experienced radiologists and were subsequently compared with histopathology results, the latter of which were reassessed by an experienced uropathologist (HO).

RESULTS: There were 54 evaluable patients (mean age 68 years, 47 [85 %] males and 7 [15 %] females) with pT and pN status as follows: < pT2-14 (26 %), pT2-10 (18 %), and > pT2-30 (56 %); pN0 37 (69 %) and pN+ 17 (31 %). PET/CT showed positive lymph nodes in 12 patients (22 %), and 7 of those cases were confirmed by histopathology; the corresponding results for conventional CT were 11 (20 %) and 7 patients (13 %), respectively. PET/CT had 41 % sensitivity, 86 % specificity, 58 % PPV, and 76 % NPV, whereas the corresponding figures for conventional CT were 41 %, 89 %, 64 %, and 77 %. Additional analyses of the right and left side of the body or in specified anatomical regions gave similar results.

CONCLUSIONS: In this study, PET/CT and conventional CT had similar low sensitivity in detecting and localizing regional lymph node metastasis in bladder cancer.

HSV kategori
Identifikatorer
urn:nbn:se:liu:diva-120796 (URN)10.1186/s12894-015-0080-z (DOI)000359832000001 ()26294219 (PubMedID)
Tilgjengelig fra: 2015-08-25 Laget: 2015-08-25 Sist oppdatert: 2017-05-17
Haj-Hosseini, N., Behm, P., Shabo, I. & Wårdell, K. (2014). Fluorescence spectroscopy using indocyanine green for lymph node mapping. In: : . Paper presented at Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XII, 2-4 February 2014, San Francisco, California, USA (pp. 1-6). , 8935(893504)
Åpne denne publikasjonen i ny fane eller vindu >>Fluorescence spectroscopy using indocyanine green for lymph node mapping
2014 (engelsk)Konferansepaper, Publicerat paper (Fagfellevurdert)
Abstract [en]

The principles of cancer treatment has for years been radical resection of the primary tumor. In the oncologic surgeries where the affected cancer site is close to the lymphatic system, it is as important to detect the draining lymph nodes for metastasis (lymph node mapping). As a replacement for conventional radioactive labeling, indocyanine green (ICG) has shown successful results in lymph node mapping; however, most of the ICG fluorescence detection techniques developed are based on camera imaging. In this work, fluorescence spectroscopy using a fiber-optical probe was evaluated on a tissue-like ICG phantom with ICG concentrations of 6-64 μM and on breast tissue from five patients. Fiber-optical based spectroscopy was able to detect ICG fluorescence at low intensities; therefore, it is expected to increase the detection threshold of the conventional imaging systems when used intraoperatively. The probe allows spectral characterization of the fluorescence and navigation in the tissue as opposed to camera imaging which is limited to the view on the surface of the tissue

Emneord
Intra-operative optical imaging, near infrared fluorescence, breast cancer surgery, optical phantom, optimal ICG concentration, spectroscopy
HSV kategori
Identifikatorer
urn:nbn:se:liu:diva-98988 (URN)10.1117/12.2036765 (DOI)000335493700002 ()9780819498489 (ISBN)
Konferanse
Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XII, 2-4 February 2014, San Francisco, California, USA
Tilgjengelig fra: 2013-10-14 Laget: 2013-10-14 Sist oppdatert: 2019-01-23bibliografisk kontrollert
Shabo, I., Olsson, H., Elkarim, R., Sun, X.-F. & Svanvik, J. (2014). Macrophage Infiltration in Tumor Stroma is Related to Tumor Cell Expression of CD163 in Colorectal Cancer. Cancer Microenvironment, 7(1), 61-69
Åpne denne publikasjonen i ny fane eller vindu >>Macrophage Infiltration in Tumor Stroma is Related to Tumor Cell Expression of CD163 in Colorectal Cancer
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2014 (engelsk)Inngår i: Cancer Microenvironment, ISSN 1875-2292, E-ISSN 1875-2284, Vol. 7, nr 1, s. 61-69Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The scavenger receptor, CD163, is a macrophage-specific marker. Recent studies have shown that CD163 expression in breast and rectal cancer cells is associated with poor prognosis. This study was conducted to evaluate the relationship between CD163 expression as a macrophage trait in cancer cells, and macrophage infiltration and its clinical significance in colorectal cancer. Immunostaining of CD163 and macrophage infiltration were evaluated in paraffin-embedded specimens, earlier analyzed for CD31, D2-40 and S-phase fraction, from primary tumors and normal colorectal mucosa of 75 patients with colorectal carcinoma. The outcomes were analyzed in relation to clinical-pathological data. CD163 expression was positive in cancer cells in 20 % of colorectal cancer patients and was related to advanced tumor stages (P = 0.008) and unfavorable prognosis (p = 0.001). High macrophage infiltration was related to shorter survival and positive CD163 expression in tumor cells. The prognostic impact of macrophage infiltration was independent of tumor stage and CD163 expression in cancer cells (p = 0.034). The expression of macrophage phenotype in colorectal cancer cells is associated with macrophage density in tumor stroma and lower survival rates. Macrophage infiltration has an independent prognostic impact on mortality in colorectal cancer. In accordance with previous experimental studies, these findings provide new insights into the role of macrophages in colorectal cancer.

sted, utgiver, år, opplag, sider
Springer Netherlands, 2014
Emneord
CD163 expression, Macrophage infiltration, Colorectal cancer
HSV kategori
Identifikatorer
urn:nbn:se:liu:diva-106894 (URN)10.1007/s12307-014-0145-7 (DOI)24771466 (PubMedID)
Tilgjengelig fra: 2014-05-23 Laget: 2014-05-23 Sist oppdatert: 2017-12-05bibliografisk kontrollert
Svanvik, J. & Shabo, I. (2014). Review and rhyme: of birth of cancers and selfish epigenomes [Letter to the editor]. Medical Hypotheses, 82(5), 639-640
Åpne denne publikasjonen i ny fane eller vindu >>Review and rhyme: of birth of cancers and selfish epigenomes
2014 (engelsk)Inngår i: Medical Hypotheses, ISSN 0306-9877, E-ISSN 1532-2777, Vol. 82, nr 5, s. 639-640Artikkel i tidsskrift, Letter (Annet vitenskapelig) Published
Abstract [en]

n/a

sted, utgiver, år, opplag, sider
Elsevier, 2014
HSV kategori
Identifikatorer
urn:nbn:se:liu:diva-112028 (URN)10.1016/j.mehy.2014.01.035 (DOI)000335105100025 ()24612784 (PubMedID)
Tilgjengelig fra: 2014-11-13 Laget: 2014-11-13 Sist oppdatert: 2017-12-05bibliografisk kontrollert
Shabo, I., Olsson, H., Stål, O. & Svanvik, J. (2013). Breast Cancer Expression of DAP12 is Associated With Skeletal and Liver Metastases and Poor Survival. Clinical Breast Cancer, 13(5), 371-377
Åpne denne publikasjonen i ny fane eller vindu >>Breast Cancer Expression of DAP12 is Associated With Skeletal and Liver Metastases and Poor Survival
2013 (engelsk)Inngår i: Clinical Breast Cancer, ISSN 1526-8209, E-ISSN 1938-0666, Vol. 13, nr 5, s. 371-377Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Macrophages are an important cellular factor in breast cancer (BRC) progression and metastasis. DNAX activating protein of 12 kD (DAP12) is essential factor for macrophage fusion function. This study was conducted to investigate the expression and significance of DAP12 expression in BRC. DAP12 is expressed in BRC cells and associated with poor survival, liver metastases, and bone metastases. These data provide new insight into the pathophysiology of macrophages in BRC. less thanbrgreater than less thanbrgreater thanBackground: The transmembrane adapter protein, DAP12, transduces activation signals for several arrays of receptors, including human signal-regulatory protein, DAP12-associating lectin-1, triggering receptor expressed on myeloid cells-1, -2, and -3, in natural killer cells, granulocytes, monocytes/macrophages, and dendritic cells. The macrophage-specific antigen, Cluster of Differentiation 163 (CD163), is expressed in breast and colorectal cancers and is associated with early cancer recurrence and poor prognosis. It was recently shown that fusion between intestinal tumor cells and macrophages results in nuclear reprogramming with hybrid transcripts from both cells of origin. The role of DAP12 in the fusion process is not known. This study investigates the expression of DAP12 in BRC cells, and its relation to other macrophage traits and to the clinical progression of disease. Materials and Methods: Immunostaining of DAP12 and CD163 was performed and evaluated in paraffin-embedded specimens from 132 patients with BRC. The outcomes were analyzed in relation to clinicopathological data. Results: DAP12 expression in cancer cells was positive in 66 percent of the cancers and was associated with high tumor grade (P = .015), and with liver (P = .047) and skeletal (P = .067), but not with lung metastases (P = 1.00). Patients with BRC expressing DAP12 had poor prognosis, with higher recurrence rates of skeletal (P = .018) and liver metastases (P = .047), and shorter survival time (P = .0060). Conclusion: We suggest that macrophage traits in BRC cells facilitate the metastatic process and that DAP12 expression might promote metastatic homing to bone and liver tissues.

sted, utgiver, år, opplag, sider
Elsevier, 2013
Emneord
Cell fusion, Tumor-associated macrophages, Tissue-specific metastasis, Tumor immunology, Tumor microenvironment
HSV kategori
Identifikatorer
urn:nbn:se:liu:diva-98662 (URN)10.1016/j.clbc.2013.05.003 (DOI)000324341600010 ()
Merknad

Funding Agencies|County Council of Ostergotland||

Tilgjengelig fra: 2013-10-10 Laget: 2013-10-10 Sist oppdatert: 2017-12-06
Shabo, I. & Svanvik, J. (2011). Expression of Macrophage Antigens by Tumor Cells. Advances in Experimental Medicine and Biology, 714, 141-150
Åpne denne publikasjonen i ny fane eller vindu >>Expression of Macrophage Antigens by Tumor Cells
2011 (engelsk)Inngår i: Advances in Experimental Medicine and Biology, ISSN 0065-2598, E-ISSN 2214-8019, Vol. 714, s. 141-150Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Macrophages are a heterogeneous cell population of the myeloid linage derived from monocytes. These cells show two different polarization states, M1 and M2 macrophages in response to different micro environmental signals. Tumor associated macrophages (TAM) represent the M2 type and promote tumor progression. These cells express antigens that more or less are specific for macrophages like: CD14, CD68, MAC387, CD 163, and DAP12. In a series of recent studies it is shown that cancer cells may express these antigens and CD I 63, MAC387 and DAP12 may be expressed by e.g. breast cancer cells. Thus, 48% of the breast cancers expressed CD163 that is a scavenger receptor normally expressed by macrophages alone. The corresponding figure for rectal cancer is 31%. The expression of CD163 is correlated to early distant recurrence in breast cancer and local recurrence in rectal cancer and reduced survival time in both conditions. Expression of macrophage antigens in breast- and colorectal-cancers may have a prognostic relevance in clinical praxis. One explanation to these findings is that resemblance with macrophages may indicate a more invasive phenotype due to genetic exchange between the primary tumor cells and associated macrophages. This is further supported by the finding that expression of DAP12, a macrophage fusion receptor, in breast cancer is associated with an advanced tumor grade and higher rates of skeletal and liver metastases and overall shorter distant recurrence free survival. Another explanation to the changed phenotype is a genetic exchange between the cells by exosome-mediated transfer.

sted, utgiver, år, opplag, sider
Springer, 2011
HSV kategori
Identifikatorer
urn:nbn:se:liu:diva-68824 (URN)10.1007/978-94-007-0782-5_7 (DOI)000290775400007 ()
Tilgjengelig fra: 2011-06-08 Laget: 2011-06-08 Sist oppdatert: 2017-12-11
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