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Lee, Chyan-Jang
Publikasjoner (6 av 6) Visa alla publikasjoner
Mak, W. C., Olesen, K., Sivlér, P., Lee, C.-J., Moreno-Jimenez, I., Edin, J., . . . Griffith, M. (2018). Correction: W.C. Mak, et al. Controlled Delivery of Human Cells by Temperature Responsive Microcapsules. J. Funct. Biomater. 2015, 6, 439-453. Journal of Functional Biomaterials, 9(2), Article ID 26.
Åpne denne publikasjonen i ny fane eller vindu >>Correction: W.C. Mak, et al. Controlled Delivery of Human Cells by Temperature Responsive Microcapsules. J. Funct. Biomater. 2015, 6, 439-453
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2018 (engelsk)Inngår i: Journal of Functional Biomaterials, ISSN 2079-4983, E-ISSN 2079-4983, Vol. 9, nr 2, artikkel-id 26Artikkel i tidsskrift (Annet vitenskapelig) Published
sted, utgiver, år, opplag, sider
Basel: MDPI, 2018
HSV kategori
Identifikatorer
urn:nbn:se:liu:diva-155826 (URN)10.3390/jfb9020026 (DOI)000446652800001 ()29561776 (PubMedID)
Tilgjengelig fra: 2019-03-29 Laget: 2019-03-29 Sist oppdatert: 2019-05-04bibliografisk kontrollert
Islam, M. M., Ravichandran, R., Olsen, D., Kozak Ljunggren, M., Fagerholm, P., Lee, C.-J., . . . Phopase, J. (2016). Self-assembled collagen-like-peptide implants as alternatives to human donor corneal transplantation. RSC Advances, 6(61), 55745-55749
Åpne denne publikasjonen i ny fane eller vindu >>Self-assembled collagen-like-peptide implants as alternatives to human donor corneal transplantation
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2016 (engelsk)Inngår i: RSC Advances, ISSN 2046-2069, E-ISSN 2046-2069, Vol. 6, nr 61, s. 55745-55749Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Extracellular matrix proteins like collagen promote regeneration as implants in clinical studies. However, collagens are large and unwieldy proteins, making small functional peptide analogs potentially ideal substitutes. Self-assembling collagen-like-peptides conjugated with PEG-maleimide were assembled into hydrogels. When tested pre-clinically as corneal implants in mini-pigs, they promoted cell and nerve regeneration, forming neo-corneas structurally and functionally similar to natural corneas.

sted, utgiver, år, opplag, sider
ROYAL SOC CHEMISTRY, 2016
HSV kategori
Identifikatorer
urn:nbn:se:liu:diva-130324 (URN)10.1039/c6ra08895c (DOI)000378275400008 ()
Merknad

Funding Agencies|Vinnova Indo-Sweden grant [2013-04645]; Integrative Regenerative Medicine Centre, Linkoping University (LiU); Region Ostergotland; Swedish Research Council grant [621-2012-4286]

Tilgjengelig fra: 2016-07-29 Laget: 2016-07-28 Sist oppdatert: 2017-11-28
Mak, W. C., Olesen, K., Sivlér, P., Lee, C.-J., Moreno- Jimenzen, I., Edin, J., . . . Griffith, M. (2015). Controlled delivery of human cells by temperature responsive microcapsules. Journal of Functional Biomaterials, 6(2), 439-453
Åpne denne publikasjonen i ny fane eller vindu >>Controlled delivery of human cells by temperature responsive microcapsules
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2015 (engelsk)Inngår i: Journal of Functional Biomaterials, ISSN 2079-4983, E-ISSN 2079-4983, Vol. 6, nr 2, s. 439-453Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Cell therapy is one of the most promising areas within regenerative medicine. However, its full potential is limited by the rapid loss of introduced therapeutic cells before their full effects can be exploited, due in part to anoikis, and in part to the adverse environments often found within the pathologic tissues that the cells have been grafted into. Encapsulation of individual cells has been proposed as a means of increasing cell viability. In this study, we developed a facile, high throughput method for creating temperature responsive microcapsules comprising agarose, gelatin and fibrinogen for delivery and subsequent controlled release of cells. We verified the hypothesis that composite capsules combining agarose and gelatin, which possess different phase transition temperatures from solid to liquid, facilitated the destabilization of the capsules for cell release. Cell encapsulation and controlled release was demonstrated using human fibroblasts as model cells, as well as a therapeutically relevant cell line—human umbilical vein endothelial cells (HUVECs). While such temperature responsive cell microcapsules promise effective, controlled release of potential therapeutic cells at physiological temperatures, further work will be needed to augment the composition of the microcapsules and optimize the numbers of cells per capsule prior to clinical evaluation.

Emneord
cell encapsulation; microcapsules; hydrogel; cell delivery; temperature responsive; human fibroblast; human umbilical vein endothelial cells
HSV kategori
Identifikatorer
urn:nbn:se:liu:diva-123654 (URN)10.3390/jfb6020439 (DOI)
Tilgjengelig fra: 2016-01-05 Laget: 2016-01-05 Sist oppdatert: 2019-02-11
Alarcon, E. I., Udekwu, K. I., Noel, C. W., Gagnon, L.-P. B. -., Taylor, P. K., Vulesevic, B., . . . Griffith, M. (2015). Safety and efficacy of composite collagen-silver nanoparticle hydrogels as tissue engineering scaffolds. Nanoscale, 7(44), 18789-18798
Åpne denne publikasjonen i ny fane eller vindu >>Safety and efficacy of composite collagen-silver nanoparticle hydrogels as tissue engineering scaffolds
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2015 (engelsk)Inngår i: Nanoscale, ISSN 2040-3364, E-ISSN 2040-3372, Vol. 7, nr 44, s. 18789-18798Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The increasing number of multidrug resistant bacteria has revitalized interest in seeking alternative sources for controlling bacterial infection. Silver nanoparticles (AgNPs), are amongst the most promising candidates due to their wide microbial spectrum of action. In this work, we report on the safety and efficacy of the incorporation of collagen coated AgNPs into collagen hydrogels for tissue engineering. The resulting hybrid materials at [AgNPs] less than0.4 mu M retained the mechanical properties and biocompatibility for primary human skin fibroblasts and keratinocytes of collagen hydrogels; they also displayed remarkable anti-infective properties against S. aureus, S. epidermidis, E. coli and P. aeruginosa at considerably lower concentrations than silver nitrate. Further, subcutaneous implants of materials containing 0.2 mu M AgNPs in mice showed a reduction in the levels of IL-6 and other inflammation markers (CCL24, sTNFR-2, and TIMP1). Finally, an analysis of silver contents in implanted mice showed that silver accumulation primarily occurred within the tissue surrounding the implant.

sted, utgiver, år, opplag, sider
Royal Society of Chemistry, 2015
HSV kategori
Identifikatorer
urn:nbn:se:liu:diva-123536 (URN)10.1039/c5nr03826j (DOI)000364824000048 ()26507748 (PubMedID)
Merknad

Funding Agencies|Natural Sciences and Engineering Research Council of Canada; NSERC/CIHR Canada; Swedish Research Council [621-2010-5189]; AFA Forsakring; University of Ottawa Heart Institute Startup grant

Tilgjengelig fra: 2015-12-21 Laget: 2015-12-21 Sist oppdatert: 2017-12-01bibliografisk kontrollert
Cheung Mak, W., Kwan Yee, C., Orban, J., Lee, C.-J., Turner, A. & Griffith, M. (2015). Surface-Engineered Contact Lens as an Advanced Theranostic Platform for Modulation and Detection of Viral Infection. ACS Applied Materials and Interfaces, 7(45), 25487-25494
Åpne denne publikasjonen i ny fane eller vindu >>Surface-Engineered Contact Lens as an Advanced Theranostic Platform for Modulation and Detection of Viral Infection
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2015 (engelsk)Inngår i: ACS Applied Materials and Interfaces, ISSN 1944-8244, E-ISSN 1944-8252, Vol. 7, nr 45, s. 25487-25494Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

We have demonstrated an entirely new concept of a wearable theranostic device in the form of a contact lens (theranostic lens) with a dual-functional hybrid surface to modulate and detect a pathogenic attack, using a the corneal HSV serotype-1 (HSV-1) model. The theranostic lenses were constructed using a facile layer-by-layer surface engineering technique, keeping the theranostic lenses with good surface wettability, optically transparency, and nontoxic toward human corneal epithelial cells. The theranostic lenses were used to capture and concentrate inflammatory cytokines such as interleukin-1 alpha (IL-1 alpha), which is upregulated during HSV-1 reactivation, for sensitive, noninvasive diagnostics. The theranostic lens also incorporated an antiviral coating to serve as a first line of defense to protect patients against disease. Our strategy tackles major problems in tear diagnostics that are mainly associated with the sampling of a relatively small volume of fluid and the low concentration of biomarkers. The theranostic lenses show effective anti-HSV-1 activity and good analytical performance for the detection of IL-1a, with a limit of detection of 1.43 pg mL(-1) and a wide linear range covering the clinically relevant region. This work offers a new paradigm for wearable noninvasive healthcare devices combining diagnosis and protection against disease, while supporting patient compliance. We believe that this approach holds immense promise as a next-generation point-of-care and decentralized diagnostic/theranostic platform for a range of biomarkers.

sted, utgiver, år, opplag, sider
American Chemical Society (ACS), 2015
Emneord
theranostics; contact lens; cornea; antiviral; diagnostics
HSV kategori
Identifikatorer
urn:nbn:se:liu:diva-123520 (URN)10.1021/acsami.5b08644 (DOI)000365148600060 ()26512953 (PubMedID)
Merknad

Funding Agencies|Linkoping Center for Life Science Technologies; Swedish Research Council [521-2012-5706]

Tilgjengelig fra: 2015-12-22 Laget: 2015-12-21 Sist oppdatert: 2017-12-01
Tengdelius, M., Lee, C.-J., Grenegård, M., Griffith, M., Påhlsson, P. & Konradsson, P. (2014). Synthesis and biological evaluation of fucoidan-mimetic glycopolymers through cyanoxyl-mediated free-radical polymerization. Biomacromolecules, 15(7), 2359-2368
Åpne denne publikasjonen i ny fane eller vindu >>Synthesis and biological evaluation of fucoidan-mimetic glycopolymers through cyanoxyl-mediated free-radical polymerization
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2014 (engelsk)Inngår i: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 15, nr 7, s. 2359-2368Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The sulfated marine polysaccharide fucoidan has been reported to have health benefits ranging from antivirus and anticancer properties to modulation of high blood pressure. Hence, they could enhance the biological function of materials for biomedical applications. However, the incorporation of fucoidan into biomaterials has been difficult, possibly due to its complex structure and lack of suitable functional groups for covalent anchoring to biomaterials. We have developed an approach for a rapid synthesis of fucoidanmimetic glycopolymer chains through cyanoxyl-mediated free-radical polymerization, a method suitable for chain-end functionalizing and subsequent linkage to biomaterials. The resulting sulfated and nonsulfated methacrylamido alpha-L-fucoside glycopolymers fucoidan-mimetic properties were studied in HSV-1 infection and platelet activation assays. The sulfated glycopolymer showed similar properties to natural fucoidan in inducing platelet activation and inhibiting HSV-1 binding and entry to cells, thus indicating successful syntheses of fucoidan-mimetic glycopolymers.

sted, utgiver, år, opplag, sider
American Chemical Society (ACS), 2014
HSV kategori
Identifikatorer
urn:nbn:se:liu:diva-109382 (URN)10.1021/bm5002312 (DOI)000339090500003 ()24813544 (PubMedID)
Tilgjengelig fra: 2014-08-15 Laget: 2014-08-15 Sist oppdatert: 2017-12-05bibliografisk kontrollert
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