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2022 (English)In: STAR Protocols, ISSN 2666-1667, Vol. 3, no 1, article id 101202Article in journal (Refereed) Published
Abstract [en]
Summary:The GBS-MeDIP protocol combines two previously described techniques, Genotype-by-Sequencing (GBS) and Methylated-DNA-Immunoprecipitation (MeDIP). Our method allows for parallel and cost-efficient interrogation of genetic and methylomic variants in the DNA of many reduced genomes, taking advantage of the barcoding of DNA samples performed in the GBS and the subsequent creation of DNA pools, then used as an input for the MeDIP. The GBS-MeDIP is particularly suitable to identify genetic and methylomic biomarkers when resources for whole genome interrogation are lacking.
Place, publisher, year, edition, pages
Cell Press, 2022
National Category
Computer and Information Sciences Biological Sciences Basic Medicine
Identifiers
urn:nbn:se:liu:diva-188044 (URN)10.1016/j.xpro.2022.101202 (DOI)
2022-09-022022-09-022023-12-28Bibliographically approved