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Jonsson, Yvonne
Publications (10 of 11) Show all publications
Persson, M., Ekerfelt, C., Jablonowska, B., Jonsson, Y., Ernerudh, J., Jenmalm, M. C. & Berg, G. (2012). Immunological status in patients undergoing in vitro fertilisation: responses to hormone treatment and relationship to outcome. Journal of Reproductive Immunology, 96(1-2), 58-67
Open this publication in new window or tab >>Immunological status in patients undergoing in vitro fertilisation: responses to hormone treatment and relationship to outcome
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2012 (English)In: Journal of Reproductive Immunology, ISSN 0165-0378, E-ISSN 1872-7603, Vol. 96, no 1-2, p. 58-67Article in journal (Refereed) Published
Abstract [en]

We aimed to prospectively investigate the paternal antigen-induced cytokine secretion by peripheral blood mononuclear cells (PBMCs) in response to hormone treatment in women undergoing in vitro fertilisation (IVF) and to examine the predictive value of the cytokine secretion profile in the outcome of IVF treatment, in a pilot study. Twenty-five women were included and IVF treatment was successful for six and unsuccessful for 19 women. Blood samples were collected before IVF treatment, on four occasions during IVF and four weeks after embryo transfer. The numbers of Th1-, Th2- and Th17-associated cytokine-secreting cells and cytokine levels in cell supernatants were analysed by enzyme-linked immunospot-forming (ELISpot), enzyme-linked immune-sorbent (ELISA) or Luminex assay. None of the cytokines (IFN-γ, IL-4, IL-5, IL-10, IL-12, IL-13, IL-17, TNF and GM-CSF) had any predictive value regarding IVF outcome. The majority of the cytokines reached their peak levels at ovum pick-up, suggesting an enhancing influence of the hormonal stimulation. Pregnancy was associated with a high number of IL-4-, IL-5- and IL-13-secreting cells four weeks after ET. In conclusion, the results do not support our hypothesis of a more pronounced peripheral Th1 and Th17 deviation towards paternal antigens in infertile women with an unsuccessful IVF outcome, although this is based on a small number of observations. A larger study is required to confirm this conclusion. Higher numbers of Th2-associated cytokine-secreting cells in pregnant women four weeks after ET do corroborate the hypothesis of a Th2 deviation during pregnancy.

Place, publisher, year, edition, pages
Elsevier, 2012
Keywords
IVF; Th1; Th2; Th17; Immune regulation
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-85640 (URN)10.1016/j.jri.2012.07.005 (DOI)000312969900007 ()
Available from: 2012-11-26 Created: 2012-11-26 Last updated: 2017-12-07Bibliographically approved
Persson, M., Ekerfelt, C., Jablonowska, B., Jonsson, Y., Berg, G., Ernerudh, J. & Jenmalm, M. C. (2012). Leukocyte populations in patients undergoing in vitro fertilization: responses to hormone treatment and relation to outcome.
Open this publication in new window or tab >>Leukocyte populations in patients undergoing in vitro fertilization: responses to hormone treatment and relation to outcome
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2012 (English)Manuscript (preprint) (Other academic)
Abstract [en]

We aimed to prospectively investigate circulating leukocyte populations in infertile women undergoing IVF treatment and to determine whether any differences in cell proportions were associated with the IVF outcome. We also assessed the effect of IVF-based ovarian stimulation on the leukocyte populations. Twenty-five women were included and IVF treatment was successful in six and unsuccessful in 19 women. Blood samples were collected before IVF treatment, at the time of embryo transfer and four weeks after embryo transfer. The numbers and proportions of lymphocytes, T cells, NK cells, monocytes and granulocytes were analysed by flow cytometry, as well as the following lymphocyte subpopulations: CD3+HLA-DR+, CD4+CD45RA+, CD4+CD45R0+, CD8+CD45RA+, CD8+CD45R0+, CD4+CD25+, CD4dimCD25bright regulatory T cells, CD3-CD56bright and CD3-CD56dim NK cells. The proportions and numbers of leukocytes during IVF treatment were not related to the IVF outcome, although pregnant women (four weeks after ET) had a lower proportion of lymphocytes than the non-pregnant women. The absolute counts of lymphocytes, T cells, granulocytes and monocytes, as well as the proportions of granulocytes and T cells, increased at the time of ET, coinciding with high FSH and hCG levels. In conclusion, the proportions and numbers of leukocyte populations were not associated with the IVF outcome, although a larger study should be conducted to confirm this conclusion. Changes in both proportions and numbers of several leukocyte populations were observed during the course of IVF treatment, suggesting a stimulatory effect of the hormonal influence.

Keywords
IVF, pregnancy, regulatory T cells, immune regulation, leukocyte populations, hormones
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-85641 (URN)
Available from: 2012-11-26 Created: 2012-11-26 Last updated: 2013-08-29
Persson, M., Ekerfelt, C., Ernerudh, J., Matthiesen, L., Sandberg, M., Jonsson, Y., . . . Jenmalm, M. C. (2012). Reduced IFN-γ and IL-10 responses to paternal antigens during and after pregnancy in allergic women. Journal of Reproductive Immunology, 95(1-2), 50-58
Open this publication in new window or tab >>Reduced IFN-γ and IL-10 responses to paternal antigens during and after pregnancy in allergic women
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2012 (English)In: Journal of Reproductive Immunology, ISSN 0165-0378, E-ISSN 1872-7603, Vol. 95, no 1-2, p. 50-58Article in journal (Refereed) Published
Abstract [en]

Normal pregnancy and allergy are both characterized by a T helper (Th) 2 deviation. In the current study, we hypothesized that paternal antigen-induced cytokine responses during pregnancy would be deviated toward Th2 and an anti-inflammatory profile, and that the Th2 deviation would be more pronounced in allergic pregnant women. Blood samples were collected longitudinally on three occasions during pregnancy and two occasions post partum (pp). Of the 86 women initially included, 54 women had a normal pregnancy and completed the sampling procedures. Twelve women fulfilled the criteria for allergy (allergic symptoms and circulating immunoglobulin [Ig] E antibodies to inhalant allergens) and 20 were non-allergic (nonsensitized without symptoms). The levels of Th1- and Th2-associated cytokines and chemokines, the Th17 cytokine IL-17 and the anti-inflammatory cytokine IL-10 of the groups were compared. Paternal antigen-induced IL-4 and IL-10 responses increased between the first and the third trimester. Allergy was associated with decreased paternal antigen-induced IFN-γ and CXCL10 secretion in the nonpregnant state (one year pp) and also decreased IFN-γ/IL-4 and IFN-γ/IL-13 ratios during pregnancy. We also observed a decreased paternal antigen-induced IL-10 response in allergic compared with non-allergic women during pregnancy, along with a decreased IL-10/IL-13 ratio. In conclusion, our findings support the hypothesis of lower Th1 responses toward paternal antigens in allergic than in non-allergic women, but also indicate that allergy is associated with a lower capacity to induce anti-inflammatory IL-10 responses after paternal antigen stimulation during pregnancy.

Place, publisher, year, edition, pages
Elsevier, 2012
Keywords
allergy, Th1/Th2, pregnancy
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-84900 (URN)10.1016/j.jri.2012.05.003 (DOI)000309090200006 ()22784413 (PubMedID)
Note

Funding Agencies|Swedish Research Council||Cancer and Allergy Association||Olle Engkvist Foundation||Vardal Foundation for Health Care Sciences and Allergy Research||National Swedish Association against Allergic Diseases||Linkoping University Hospital||

Available from: 2012-10-26 Created: 2012-10-26 Last updated: 2017-12-07
Persson, M., Ekerfelt, C., Jablonowska, B., Jonsson, Y., Ernerudh, J., Jenmalm, M. & Berg, G. (2011). Cytokine networks for implantation and early pregnancy: immunologic status in patients undergoing in vitro fertilization in JOURNAL OF REPRODUCTIVE IMMUNOLOGY, vol 90, issue 2, pp 166-166. In: JOURNAL OF REPRODUCTIVE IMMUNOLOGY (pp. 166-166). Elsevier, 90(2)
Open this publication in new window or tab >>Cytokine networks for implantation and early pregnancy: immunologic status in patients undergoing in vitro fertilization in JOURNAL OF REPRODUCTIVE IMMUNOLOGY, vol 90, issue 2, pp 166-166
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2011 (English)In: JOURNAL OF REPRODUCTIVE IMMUNOLOGY, Elsevier , 2011, Vol. 90, no 2, p. 166-166Conference paper, Published paper (Refereed)
Abstract [en]

n/a

Place, publisher, year, edition, pages
Elsevier, 2011
Keywords
IVF, Th1/Th2, Cytokines, Immune regulation
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-70342 (URN)10.1016/j.jri.2011.06.063 (DOI)000293873500059 ()
Available from: 2011-09-02 Created: 2011-09-02 Last updated: 2013-08-29
Sandberg, M., Frykman, A., Jonsson, Y., Persson, M., Ernerudh, J., Berg, G., . . . Jenmalm, M. (2009). Total and allergen-specific IgE levels during and after pregnancy in relation to maternal allergy. JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 81(1), 82-88
Open this publication in new window or tab >>Total and allergen-specific IgE levels during and after pregnancy in relation to maternal allergy
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2009 (English)In: JOURNAL OF REPRODUCTIVE IMMUNOLOGY, ISSN 0165-0378, Vol. 81, no 1, p. 82-88Article in journal (Refereed) Published
Abstract [en]

Type 2 T-helper cell (Th2)-skewed immunity is associated with successful pregnancy and the ability to easily direct immune responses to a Th2-polarised profile may be an evolutionary benefit. The Th2-like immunity associated with allergic disease might generate favourable effects for the maintenance of pregnancy, but could also promote development of Th2-like immune responses and allergic disease in the offspring. The aim of this study was to explore, by using IgE as a stable proxy for Th2, the Th1/Th2 balance in allergic and non-allergic women by measuring allergen-specific and total IgE antibody levels in plasma during pregnancy and after delivery. Specific and total IgE antibody levels were determined by ImmunoCAP technology at five occasions during pregnancy (gestational weeks 10-12, 15-16, 25, 35 and 39), as well as at 2 and 12 months after delivery. Thirty-six women without and 20 women with allergic symptoms were included, of whom 13 were sensitised with allergic symptoms and 30 were non-sensitised without allergic symptoms. The levels of total IgE, but not allergen-specific IgE, were increased during early pregnancy when compared to 12 months after delivery in the sensitised women with allergic symptoms, but not in the non-sensitised women without allergic symptoms (pandlt;0.01). This increase in total IgE levels during early pregnancy only in the sensitised women with allergic symptoms indicates that allergy is associated with an enhanced Th2 deviation during pregnancy.

Keywords
Allergy, IgE, Phadiatop, Pregnancy, Th2
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-19894 (URN)10.1016/j.jri.2009.04.003 (DOI)
Note

Original Publication: Martina Sandberg, Anne Frykman, Yvonne Jonsson, Marie Persson, Jan Ernerudh, Göran Berg, Leif Matthiesen, Christina Ekerfelt and Maria Jenmalm, Total and allergen-specific IgE levels during and after pregnancy in relation to maternal allergy, 2009, JOURNAL OF REPRODUCTIVE IMMUNOLOGY, (81), 1, 82-88. http://dx.doi.org/10.1016/j.jri.2009.04.003 Copyright: Elsevier Science B.V., Amsterdam. http://www.elsevier.com/

Available from: 2009-09-09 Created: 2009-08-14 Last updated: 2014-04-29Bibliographically approved
Persson, M., Ekerfelt, C., Ernerudh, J., Matthiesen, L., Jenmalm, M., Jonsson, Y., . . . Berg, G. (2008). Increased circulating paternal antigen-specific IFN-γ- and IL-4-secreting cells during pregnancy in allergic and non-allergic women. Journal of Reproductive Immunology, 79(1), 70-78
Open this publication in new window or tab >>Increased circulating paternal antigen-specific IFN-γ- and IL-4-secreting cells during pregnancy in allergic and non-allergic women
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2008 (English)In: Journal of Reproductive Immunology, ISSN 0165-0378, E-ISSN 1872-7603, Vol. 79, no 1, p. 70-78Article in journal (Refereed) Published
Abstract [en]

INTRODUCTION: Allergic women have been reported to give birth to more children than non-allergic women, speculatively explained by the former's predisposition for Th2 polarization, possibly favoring pregnancy.

AIM: The aim of this study was to test the hypothesis that allergy is associated with more Th2-deviated responses to paternal antigens throughout pregnancy.

METHODS: Blood samples were collected on six occasions during pregnancy and two occasions postpartum (pp). Of the 86 women initially included, 54 women had a normal pregnancy and completed the sampling procedures. Eleven women fulfilled the strict criteria for allergy (allergic symptoms and circulating IgE antibodies to inhalant allergens) and 23 were strictly non-allergic (non-sensitized without symptoms). The numbers of blood mononuclear cells secreting IFN-gamma and IL-4, spontaneously and in response to paternal alloantigens, were compared between the groups.

RESULTS: The numbers of spontaneously as well as paternal antigen-induced IFN-gamma- and IL-4-secreting cells were similar in allergic and non-allergic pregnant women on all occasions. A similar increase in the numbers of both IFN-gamma- and IL-4-secreting cells were found in allergic and non-allergic women during pregnancy, both regarding spontaneous and paternal antigen-induced secretion.

CONCLUSIONS: This study does not support the hypothesis of a more pronounced Th2-deviation to paternal antigens in allergic pregnant women compared with non-allergic pregnant women, as measured by number of cytokine-secreting cells. The observed increase of both IFN-gamma- and IL-4-secreting cells during normal pregnancy may be interpreted as a Th2-situation, since the effects of IL-4 predominate over the effects of IFN-gamma.

Keywords
Pregnancy, Th2, Elispot, MLC
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-16134 (URN)10.1016/j.jri.2008.07.001 (DOI)000260989000010 ()18752853 (PubMedID)
Available from: 2009-01-08 Created: 2009-01-07 Last updated: 2017-12-14
Jonsson, Y., Rubér, M., Matthiesen, L., Berg, G., Nieminen, K., Sharma, S., . . . Ekerfelt, C. (2006). Cytokine mapping of sera from women with preeclampsia and from women with normal pregnancies. Journal of Reproductive Immunology, 70(1-2), 83-91
Open this publication in new window or tab >>Cytokine mapping of sera from women with preeclampsia and from women with normal pregnancies
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2006 (English)In: Journal of Reproductive Immunology, ISSN 0165-0378, Vol. 70, no 1-2, p. 83-91Article in journal (Refereed) Published
Abstract [en]

Introduction

Preeclampsia is a pregnancy-specific syndrome. The immune system in preeclampsia is changed with an increased innate activity and there is a hypothesis of a shift towards Th1-type immunity. The aim of this study was to determine a spectrum of soluble immunological factors denoting different aspects of immune activation in third trimester sera from women with preeclampsia (N = 15) and compare with levels in sera from normal pregnant women (N = 15).

Material and methods

IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12 p40, IL-13, IL-15, IL-17, IFN-α, IFN-γ, TNF-α, GM-CSF, MIP-lα, MIP-1β, MCP-1, eotaxin and RANTES were measured in serum using multiplex bead arrays. The levels of soluble CD14 and soluble IL-4 receptor were measured by enzyme-linked immunoassay (ELISA).

Results

Preeclamptic women had significantly increased levels of circulating IL-6 (p = 0.002), IL-8 (p = 0.003) and soluble IL-4R (p = 0.037), compared to women with normal pregnancies.

Conclusion

This study supports the hypothesis of increased inflammatory responses in preeclampsia, illustrated by the increased levels of IL-6 and IL-8. The finding of increased levels of soluble IL-4 receptor is an intriguing finding with several interpretations, which may partly support the hypothesis of a Th1 shift in preeclampsia.

Keywords
Cytokines; Serum; Multiple bead array; Preeclampsia
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-14374 (URN)10.1016/j.jri.2005.10.007 (DOI)
Available from: 2007-03-23 Created: 2007-03-23 Last updated: 2013-08-29
Jonsson, Y. (2005). Cytokines and immune balance in preeclampsia: a survey of some immunological variables and methods in the study of preeclampsia. (Doctoral dissertation). Institutionen för molekylär och klinisk medicin
Open this publication in new window or tab >>Cytokines and immune balance in preeclampsia: a survey of some immunological variables and methods in the study of preeclampsia
2005 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Preeclampsia is one of the most feared pregnancy complications, with a risk of maternal and fetal death and with no ideal therapy readily available. The cause of this strictly pregnancyrelated disease is still unknown and is therefore a great challenge to all researchers in the field of pregnancy-related pathophysiology.

Today, the dominating theory of the origin of preeclampsia is defective initial placentation with insufficient penetration of the trophoblasts, leading to impaired maternal blood flow through narrow spiral arteries. However, the cause of this defective trophoblast behavior is not known. The maternal immune system has been proposed to have an influence on both the placentation and the subsequent systemic reactions. Therefore, it is very interesting to study the maternal immune system during preeclampsia, in hope of achieving a better understanding of this puzzling disease.

Earlier studies have suggested that normal pregnancy requires a shift to a Th2/antiinflammatory type of immunity, at least directed towards the fetus and placenta, while some pregnancy complications, such as preeclampsia, could be due to a skewed Th1/proinflammatory type of immunity. However, the results from earlier studies designed to test the Th1/Th2 hypothesis in preeclampsia have not been consistent. Therefore, the aim of this thesis was to examine if established preeclampsia is associated with increased innate inflammatory responses and a deviation of adaptive responses towards Th1 when compared with normal pregnancy.

Enumerations of cytokine-producing cells from peripheral blood did not show any difference in the production of IFN-γ, IL-4, IL-10 and IL-12 between women with preeclampsia and normal pregnancies. However, a decrease in the spontaneously produced levels of IL-5 was detected in cell cultures on peripheral blood mononuclear cells in women with preeclampsia. Furthermore, a decreased production of IL-10 in response to paternal antigens, believed to represent the fetus, was also detected for the preeclamptic women.

Serum analysis showed increased levels of the pro-inflammatory mediators IL-6 and IL-8 during preeclampsia. Also, preeclamptic women displayed increased serum levels of the soluble IL-4 receptor, but no difference in the levels of IL-4 compared to normal pregnant women. This was an elusive finding, since the receptor was originally thought to reflect the levels of IL-4, but has recently been shown to have both agonistic and antagonistic properties on the IL-4 levels. Further studies of the local immune responses in the placenta showed no difference in the immunohistochemical staining of IL-4 and TNF-α between women with preeclampsia and women with normal pregnancies. In general, there were no hallmarks of abnormal morphology in the placental sections examined, regardless of diagnosis.

In conclusion, the decreased levels of IL-10 in response to paternal antigens and the systemically increased levels of IL-6 and IL-8 suggest a specific decrease in antiinflammatory responses towards fetal antigens, together with a systemic activation of proinflammatory mediators during preeclampsia. Furthermore, the decreased production of IL-5 also indicates, at least partly, decreased Th2 responses in the established preeclampsia.

Place, publisher, year, edition, pages
Institutionen för molekylär och klinisk medicin, 2005
Series
Linköping University Medical Dissertations, ISSN 0345-0082 ; 924
Keywords
Preeclampsia, Cytokines, Leukocytes, Immunology, Placenta, Th1/Th2 balance, Inflammation
National Category
Immunology in the medical area
Identifiers
urn:nbn:se:liu:diva-8602 (URN)91-85497-60-6 (ISBN)
Public defence
2005-12-09, Berzeliussalen, Campus Valla, Linköpings universitet, Linköping, 13:00 (English)
Opponent
Supervisors
Note
Figure 1 on page 6 is republished in the Ph.D. thesis with the kind permisson of Blackwell Publishing (http://www.blackwellpublishing.com). Figure IX on page38, figure XB on page 41, figure XI on page 46 and figure XII on page 47 are all published in the Journal of Reproductive Immunology and republished with kind permisson from Elsevier (http://www.elsevier.com/) in the Ph.D. thesis.Available from: 2007-03-23 Created: 2007-03-23 Last updated: 2018-01-13
Matthiesen, L., Berg, G., Ernerudh, J., Ekerfelt, C., Jonsson, Y. & Sharma, S. (2005). Immunology of preeclampsia. In: Markert U.R. (Jena) (Ed.), Immunology of Pregnancy: (pp. 49-61). Basel, Switzerland: S. Karger, 89
Open this publication in new window or tab >>Immunology of preeclampsia
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2005 (English)In: Immunology of Pregnancy / [ed] Markert U.R. (Jena), Basel, Switzerland: S. Karger, 2005, Vol. 89, p. 49-61Chapter in book (Refereed)
Abstract [en]

Preeclampsia is a placenta-dependent disorder with both local and systemic anomalies with neonatal and maternal morbidity. It is manifested late in pregnancy, but the onset is during early stages of gestation. The current hypothesis regarding the aetiology of preeclampsia is focused on maladaptation of immune responses and defective trophoblast invasion. Thus, an excessive maternal inflammatory response, perhaps directed against foreign fetal antigens, results in a chain of events including shallow trophoblast invasion, defective spiral artery remodelling, placental infarction and release of pro-inflammatory cytokines and placental fragments in the systemic circulation. During normal pregnancy, trophoblasts interact in the decidua with the unique uterine NK cells, modifying their cytokine repertoire, regulating adhesion molecules and matrix metalloproteinases. The inability of trophoblasts to accomplish these changes might be a critical factor for the onset of preeclampsia. Several cytokines, produced at the maternal-fetal interface, have an impact on trophoblast invasion. It is suggested that deficiency of interleukin-10 may contribute to enhanced inflammatory responses towards the trophoblasts elicited by e.g. tumour necrosis factor-α and interferon-γ. Consequently, trophoblasts subjected to a high rate of apoptosis are hampered in their invasive capacity resulting in defective transformation of spiral arteries, hypoxia, thrombosis and infarction of the placenta. The ensuing infarction of placenta leads to leakage of increasing amounts of placental fragments and cytokines in the maternal circulation and an exaggerated systemic endothelial activation as identified in preeclampsia. So far, treatment of preeclampsia is focused on signs like hypertension, whereas attempts of modifying immune responses may be a possibility in the future.

Place, publisher, year, edition, pages
Basel, Switzerland: S. Karger, 2005
Series
Chemical Immunology and Allergy, ISSN 1660-2242 ; Vol. 89
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-29576 (URN)10.1159/000087912 (DOI)16129952 (PubMedID)14952 (Local ID)978-3-8055-7970-4 (ISBN)978-3-318-01248-4 (ISBN)14952 (Archive number)14952 (OAI)
Available from: 2009-10-09 Created: 2009-10-09 Last updated: 2014-06-18Bibliographically approved
Jonsson, Y., Matthiesen, L., Berg, G., Ernerudh, J., Nieminen, K. & Ekerfelt, C. (2005). Indications of an altered immune balance in preeclampsia: A decrease in in vitro secretion of IL-5 and IL-10 from blood mononuclear cells and in blood basophil counts compared with normal pregnancy. Journal of Reproductive Immunology, 66(1), 69-84
Open this publication in new window or tab >>Indications of an altered immune balance in preeclampsia: A decrease in in vitro secretion of IL-5 and IL-10 from blood mononuclear cells and in blood basophil counts compared with normal pregnancy
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2005 (English)In: Journal of Reproductive Immunology, ISSN 0165-0378, Vol. 66, no 1, p. 69-84Article in journal (Refereed) Published
Abstract [en]

It has been suggested that maladaptation of the maternal immune response during pregnancy might be a causal factor for preeclampsia. This study was designed to examine the systemic immune status at both the innate level and the adaptive level in pregnancies complicated by preeclampsia (n = 15) and normal pregnancies (n = 15). Spontaneous and in vitro-induced secretion of IL-5, IL-6, IL-10, IL-12, IL-13 and TNF-α, in response to paternal blood cells and the vaccination antigens purified protein derivate of tuberculin (PPD) and tetanus toxoid (TT), was detected in cell culture supernatants from blood mononuclear cells by ELISA. Preeclamptic women showed reduced numbers of basophil granulocytes in the blood (p = 0.004) and lower spontaneous secretion of IL-5 from blood mononuclear cells (p = 0.016). In addition, paternal antigen-induced secretion of IL-10 was decreased in preeclampsia compared with normal pregnancy (p = 0.012). No further differences between preeclampsia and normal pregnancy were found for any stimuli or cytokines. The present findings of reduced basophil numbers and lower spontaneous in vitro secretion of IL-5 in preeclampsia compared with normal pregnancy indicate a decrease in systemic Th2 immunity in preeclampsia. Furthermore, the decrease in paternal antigen-induced secretion of the immunosuppressive cytokine IL-10 in preeclampsia indicates a fetus-specific decrease in immunosuppression mediated by blood mononuclear cells. Whether these systemic changes are a cause or a consequence of preeclampsia remains to be elucidated.

Keywords
Cytokines; Preeclampsia; In vitro; ELISA; Th1/Th2
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-14373 (URN)10.1016/j.jri.2005.02.002 (DOI)
Available from: 2007-03-23 Created: 2007-03-23 Last updated: 2013-08-29
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