liu.seSearch for publications in DiVA
Change search
Link to record
Permanent link

Direct link
BETA
Ghafouri, Bijar
Publications (10 of 51) Show all publications
Ljunggren, S., Bengtsson, T., Karlsson, H., Starkhammar Johansson, C., Palm, E., Nayeri, F., . . . Lönn, J. (2019). Modified lipoproteins in periodontitis: a link to cardiovascular disease?. Bioscience Reports, 39(3), Article ID BSR20181665.
Open this publication in new window or tab >>Modified lipoproteins in periodontitis: a link to cardiovascular disease?
Show others...
2019 (English)In: Bioscience Reports, ISSN 0144-8463, E-ISSN 1573-4935, Vol. 39, no 3, article id BSR20181665Article in journal (Refereed) Published
Abstract [en]

There is a strong association between periodontal disease and atherosclerotic cardiovascular disorders. A key event in the development of atherosclerosis is accumulation of modified lipoproteins within the arterial wall. We hypothesise that patients with periodontitis have an altered lipoprotein profile towards an atherogenic form. Therefore, the present study aims at identifying modifications of plasma lipoproteins in periodontitis. Lipoproteins from ten female patients with periodontitis and gender- and age-matched healthy controls were isolated by density-gradient ultracentrifugation. Proteins were separated by 2D gel-electrophoresis and identified by map-matching or by nano-LC followed by MS. Apolipoprotein (Apo) A-I (ApoA-I) methionine oxidation, Oxyblot, total antioxidant capacity and a multiplex of 71 inflammation-related plasma proteins were assessed. Reduced levels of apoJ, phospholipid transfer protein, apoF, complement C3, paraoxonase 3 and increased levels of alpha-1-antichymotrypsin, apoA-II, apoC-III were found in high-density lipoprotein (HDL) from the patients. In low-density lipoprotein (LDL)/very LDL (VLDL), the levels of apoL-1 and platelet-activating factor acetylhydrolase (PAF-AH) as well as apo-B fragments were increased. Methionine oxidation of apoA-I was increased in HDL and showed a relationship with periodontal parameters. alpha-1 antitrypsin and alpha-2-HS glycoprotein were oxidised in LDL/VLDL and antioxidant capacity was increased in the patient group. A total of 17 inflammation-related proteins were important for group separation with the highest discriminating proteins identified as IL-21, Fractalkine, IL-17F, IL-7, IL-1RA and IL-2. Patients with periodontitis have an altered plasma lipoprotein profile, defined by altered protein levels as well as post-translational and other structural modifications towards an atherogenic form, which supports a role of modified plasma lipoproteins as central in the link between periodontal and cardiovascular disease (CVD).

Place, publisher, year, edition, pages
Portland Press, 2019
National Category
Cardiac and Cardiovascular Systems
Identifiers
urn:nbn:se:liu:diva-157252 (URN)10.1042/BSR20181665 (DOI)000465453700016 ()30842338 (PubMedID)2-s2.0-85063936955 (Scopus ID)
Note

Funding Agencies|Swedish Knowledge Foundation [Dnr20150037]; Foundation Langmanska Kulturfonden; Magnus Bergwalls Foundation

Available from: 2019-06-04 Created: 2019-06-04 Last updated: 2019-06-10Bibliographically approved
Mayo, L. M., Asratian, A., Lindé, J., Holm, L., Nätt, D., Augier, G., . . . Heilig, M. (2018). Protective effects of elevated anandamide on stress and fear-related behaviors: translational evidence from humans and mice. Molecular Psychiatry
Open this publication in new window or tab >>Protective effects of elevated anandamide on stress and fear-related behaviors: translational evidence from humans and mice
Show others...
2018 (English)In: Molecular Psychiatry, ISSN 1359-4184, E-ISSN 1476-5578Article in journal (Refereed) Epub ahead of print
Abstract [en]

Post-traumatic stress disorder (PTSD) is a common, debilitating condition with limited treatment options. Extinction of fear memories through prolonged exposure therapy, the primary evidence-based behavioral treatment for PTSD, has only partial efficacy. In mice, pharmacological inhibition of fatty acid amide hydrolase (FAAH) produces elevated levels of anandamide (AEA) and promotes fear extinction, suggesting that FAAH inhibitors may aid fear extinction-based treatments. A human FAAH 385C-greater thanA substitution encodes an FAAH enzyme with reduced catabolic efficacy. Individuals homozygous for the FAAH 385A allele may therefore offer a genetic model to evaluate the impact of elevations in AEA signaling in humans, helping to inform whether FAAH inhibitors have the potential to facilitate fear extinction therapy for PTSD. To overcome the challenge posed by low frequency of the AA genotype (appr. 5%), we prospectively genotyped 423 individuals to examine the balanced groups of CC, AC, and AA individuals (n = 25/group). Consistent with its loss-of-function nature, the A allele was dose dependently associated with elevated basal AEA levels, facilitated fear extinction, and enhanced the extinction recall. Moreover, the A-allele homozygotes were protected against stress-induced decreases in AEA and negative emotional consequences of stress. In a humanized mouse model, AA homozygous mice were similarly protected against stress-induced decreases in AEA, both in the periphery, and also in the amygdala and prefrontal cortex, brain structures critically involved in fear extinction and regulation of stress responses. Collectively, these data suggest that AEA signaling can temper aspects of the stress response and that FAAH inhibition may aid the treatment for stress-related psychiatric disorders, such as PTSD.

Place, publisher, year, edition, pages
Nature Publishing Group, 2018
National Category
Neurosciences
Identifiers
urn:nbn:se:liu:diva-154914 (URN)10.1038/s41380-018-0215-1 (DOI)30120421 (PubMedID)2-s2.0-85052287102 (Scopus ID)
Available from: 2019-03-05 Created: 2019-03-05 Last updated: 2019-05-01Bibliographically approved
Kallman, T. F., Ghafouri, B. & Bäckryd, E. (2018). Salivary beta-endorphin and substance P are not biomarkers of neuropathic chronic pain propensity. Heliyon, 4(8), Article ID e00718.
Open this publication in new window or tab >>Salivary beta-endorphin and substance P are not biomarkers of neuropathic chronic pain propensity
2018 (English)In: Heliyon, ISSN 2405-8440, Vol. 4, no 8, article id e00718Article in journal (Refereed) Published
Abstract [en]

Objective

The pathophysiology of chronic pain is complex, with most of our knowledge being derived from preclinical studies. The search for biomarkers mirroring the pathophysiology of chronic pain is ongoing, and there is an increasing interest in saliva as a diagnostic tool. Given what is known about salivary substance Pand salivary gland innervation, we hypothesized that salivary substance P and/or beta-endorphin might reflect the basal activity of these neuropeptides in the central nervous system, thereby perhaps mirroring a general propensity to chronic pain. Based on this overall hypothesis, our aim was to compare salivary levels of these neuropeptides in chronic neuropathic pain patients with healthy controls. An additional aim was to relate salivary levels to plasma levels.

Materials and methods

We compared salivary concentrations of beta-endorphin and substance P in 14 chronic neuropathic pain patients with concentrations in 18 healthy controls using a Luminex technology kit. Salivary-to-plasma quotients were also calculated.

Results

We found no significant difference between the groups' salivary concentrations of substance P and beta-endorphin. No correlation was found between salivary and plasma concentrations of each neuropeptide, which we hypothesize might point to local production of beta-endorphin and/or substance P in the salivary glands. Given high substance P salivary-to-plasma quotients, such a local production seems more likely for substance P than for beta-endorphin.

Conclusions

Propensity to neuropathic chronic pain was not substantiated by our analysis of salivary levels of substance P and/or beta-endorphin. However, we report salivary-to-plasma quotients that give potentially important physiological insight about these neuropeptides.

Place, publisher, year, edition, pages
Elsevier, 2018
Keywords
Neurology, Neuroscience
National Category
Anesthesiology and Intensive Care
Identifiers
urn:nbn:se:liu:diva-154915 (URN)10.1016/j.heliyon.2018.e00718 (DOI)000443512800046 ()30116793 (PubMedID)2-s2.0-85050880433 (Scopus ID)
Available from: 2019-03-05 Created: 2019-03-05 Last updated: 2019-03-14Bibliographically approved
Ghafouri, B., Carlsson, A., Holmberg, S., Thelin, A. & Tagesson, C. (2016). Biomarkers of lsystemic inflammation in farmers with musculoskeletal disorders; a plasma proteomic study. BMC Musculoskeletal Disorders, 17(206)
Open this publication in new window or tab >>Biomarkers of lsystemic inflammation in farmers with musculoskeletal disorders; a plasma proteomic study
Show others...
2016 (English)In: BMC Musculoskeletal Disorders, ISSN 1471-2474, E-ISSN 1471-2474, Vol. 17, no 206Article in journal (Refereed) Published
Abstract [en]

Background: Farmers have an increased risk for musculoskeletal disorders (MSD) such as osteoarthritis of the hip, low back pain, and neck and upper limb complaints. The underlying mechanisms are not fully understood. Workrelated exposures and inflammatory responses might be involved. Our objective was to identify plasma proteins that differentiated farmers with MSD from rural referents. Methods: Plasma samples from 13 farmers with MSD and rural referents were included in the investigation. Gel based proteomics was used for protein analysis and proteins that differed significantly between the groups were identified by mass spectrometry. Results: In total, 15 proteins differed significantly between the groups. The levels of leucine-rich alpha-2-glycoprotein, haptoglobin, complement factor B, serotransferrin, one isoform of kininogen, one isoform of alpha-1-antitrypsin, and two isoforms of hemopexin were higher in farmers with MSD than in referents. On the other hand, the levels of alpha-2-HS-glycoprotein, alpha-1B-glycoprotein, vitamin D-binding protein, apolipoprotein A1, antithrombin, one isoform of kininogen, and one isoform of alpha-1-antitrypsin were lower in farmers than in referents. Many of the identified proteins are known to be involved in inflammation. Conclusions: Farmers with MSD had altered plasma levels of protein biomarkers compared to the referents, indicating that farmers with MSD may be subject to a more systemic inflammation. It is possible that the identified differences of proteins may give clues to the biochemical changes occurring during the development and progression of MSD in farmers, and that one or several of these protein biomarkers might eventually be used to identify and prevent work-related MSD.

Place, publisher, year, edition, pages
BIOMED CENTRAL LTD, 2016
Keywords
Musculoskeletal disorder; Farmers; Proteomic; Systemic inflammation; Occupational medicine
National Category
Basic Medicine
Identifiers
urn:nbn:se:liu:diva-128936 (URN)10.1186/s12891-016-1059-y (DOI)000375761500001 ()27160764 (PubMedID)
Note

Funding Agencies|Swedish Farmers Foundation for Agricultural Research [H0935072]; Swedish Rheumatism Association [R-420491]

Available from: 2016-06-09 Created: 2016-06-07 Last updated: 2018-01-10
Ernberg, M., Christidis, N., Ghafouri, B., Bileviciute-Ljungar, I., Löfgren, M., Larsson, A., . . . Gerdle, B. (2016). Effects of 15 weeks of resistance exercise on pro-inflammatory cytokine levels in the vastus lateralis muscle of patients with fibromyalgia. Arthritis Research & Therapy, 18(137)
Open this publication in new window or tab >>Effects of 15 weeks of resistance exercise on pro-inflammatory cytokine levels in the vastus lateralis muscle of patients with fibromyalgia
Show others...
2016 (English)In: Arthritis Research & Therapy, ISSN 1478-6354, E-ISSN 1478-6362, Vol. 18, no 137Article in journal (Refereed) Published
Abstract [en]

Background: This study aimed at investigating the effect of a resistance exercise intervention on the interstitial muscle levels of pro-inflammatory cytokines in fibromyalgia (FMS) and healthy controls (CON). Methods: Twenty-four female patients with FMS (54 +/- 8 years) and 27 female CON (54 +/- 9 years) were subjected to intramuscular microdialysis of the most painful vastus lateralis muscle before and after 15 weeks of progressive resistance exercise twice per week. Baseline dialysates were sampled in the resting muscle 140 min after insertion of the microdialysis catheter. The participants then performed repetitive dynamic contractions (knee extension) for 20 min, followed by 60 min rest. Pain intensity was assessed with a 0-100 mm visual analogue scale (VAS), and fatigue was assessed with Borgs RPE throughout microdialysis. Dialysates were sampled every 20 min and analyzed with Luminex for interleukin (IL)-1 beta, tumor necrosis factor (TNF) alpha, IL-6, and IL-8. Results: At both sessions and for both groups the dynamic contractions increased pain (P amp;lt; 0.012) and fatigue (P amp;lt; 0.001). The levels of TNF were lower in the FMS group than the CON group at both sessions (P amp;lt; 0.05), but none of the other cytokines differed between the groups. IL-6 and IL-8 increased after the dynamic contractions in both groups (P amp;lt; 0.010), while TNF increased only in CON (P amp;lt; 0.05) and IL-1 beta did not change. Overall pain intensity was reduced after the 15 weeks of resistance exercise in FMS (P amp;lt; 0.05), but there was no changes in fatigue or cytokine levels. Conclusion: Progressive resistance exercise for 15 weeks did not affect the interstitial levels of IL-1 beta, TNF, IL-6, and IL-8 in the vastus lateralis muscle of FMS patients or CON.

Place, publisher, year, edition, pages
BIOMED CENTRAL LTD, 2016
Keywords
Cytokines; Dynamic contractions; Exercise; Fibromyalgia; Microdialysis; Quadriceps
National Category
Rheumatology and Autoimmunity
Identifiers
urn:nbn:se:liu:diva-130286 (URN)10.1186/s13075-016-1041-y (DOI)000377977300001 ()27296860 (PubMedID)
Note

Funding Agencies|Swedish Rheumatism Association; Swedish Research Council [K2009-52P-20943-03-2, K2011-69X-21874-01-6, K2015-99X-21874-05-05]; Stockholm County Council (ALF); County Council of Ostergotland (ALF); AFA insurance; Norrbacka-Eugenia foundation; Health and Medical Care Executive Board of Vastra Gotaland Region; ALF-LUA at Sahlgrenska University Hospital; Gothenburg Center for Person Centered Care (GPCC)

Available from: 2016-08-01 Created: 2016-07-28 Last updated: 2017-11-28
Gerdle, B., Ernberg, M., Mannerkorpi, K., Larsson, B., Kosek, E., Christidis, N. & Ghafouri, B. (2016). Increased Interstitial Concentrations of Glutamate and Pyruvate in Vastus Lateralis of Women with Fibromyalgia Syndrome Are Normalized after an Exercise Intervention: a Case-Control Study. PLoS ONE, 11(10), Article ID e0162010.
Open this publication in new window or tab >>Increased Interstitial Concentrations of Glutamate and Pyruvate in Vastus Lateralis of Women with Fibromyalgia Syndrome Are Normalized after an Exercise Intervention: a Case-Control Study
Show others...
2016 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 11, no 10, p. 14article id e0162010Article in journal (Refereed) Published
Abstract [en]

Background Fibromyalgia syndrome (FMS) is associated with central alterations, but controversies exist regarding the presence and role of peripheral factors. Microdialysis (MD) can be used in vivo to study muscle alterations in FMS. Furthermore for chronic pain conditions such as FMS, the mechanisms for the positive effects of exercise are unclear. This study investigates the interstitial concentrations of algesics and metabolites in the vastus lateralis muscle of 29 women with FMS and 28 healthy women before and after an exercise intervention. Methods All the participants went through a clinical examination and completed a questionnaire. In addition, their pressure pain thresholds (PPTs) in their upper and lower extremities were determined. For both groups, MD was conducted in the vastus lateralis muscle before and after a 15-week exercise intervention of mainly resistance training of the lower limbs. Muscle blood flow and interstitial muscle concentrations of lactate, pyruvate, glutamate, glucose, and glycerol were determined. Results FMS was associated with significantly increased interstitial concentrations of glutamate, pyruvate, and lactate. After the exercise intervention, the FMS group exhibited significant decreases in pain intensity and in mean interstitial concentrations of glutamate, pyruvate, and glucose. The decrease in pain intensity in FMS correlated significantly with the decreases in pyruvate and glucose. In addition, the FMS group increased their strength and endurance. Conclusion This study supports the suggestion that peripheral metabolic and algesic muscle alterations are present in FMS patients and that these alterations contribute to pain. After an exercise intervention, alterations normalized, pain intensity decreased (but not abolished), and strength and endurance improved, all findings that suggest the effects of exercise are partially peripheral.

Place, publisher, year, edition, pages
Public Library of Science, 2016. p. 14
National Category
Clinical Medicine Public Health, Global Health, Social Medicine and Epidemiology Nursing
Identifiers
urn:nbn:se:liu:diva-131423 (URN)10.1371/journal.pone.0162010 (DOI)000385553100010 ()
Note

Funding agencies:

This work was funded by the Swedish Rheumatism Association, the Swedish Research Council, the Health and Medical Care Executive Board of Vastra Gotaland Region, ALF-LUA at Sahlgrenska University Hospital and Linkbping University Hospital, Linkbping University, Gothenburg Centre for Person Centred Care (GPCC) and AFA Insurance. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Available from: 2016-09-02 Created: 2016-09-02 Last updated: 2017-11-21Bibliographically approved
Hadrévi, J., Turkina, M., Carlsson, A., Gerdle, B., Larsson, B., Hellström, F. & Ghafouri, B. (2016). Myosin light chain and calcium regulating protein differences in chronic musculoskeletal neck and shoulder pain. Journal of Integrated OMICS, 6(1), 1-8
Open this publication in new window or tab >>Myosin light chain and calcium regulating protein differences in chronic musculoskeletal neck and shoulder pain
Show others...
2016 (English)In: Journal of Integrated OMICS, ISSN 2182-0287, Vol. 6, no 1, p. 1-8Article in journal (Refereed) Published
Abstract [en]

Proteomic screening analysis has detected myosin light chain (MLC) as a protein implied to be involved in chronic musculoskeletal neck and shoulder pain. Several analyses of MLC proteins have stated a difference in phosphorylation being the determining factor for protein activation hence altered contrability of the muscle in i.e. senescence. In continuation of a previous publication, this study is an attempt to analyze the different MLC isoforms by mass spectrometry and immune-analyses in myalgic and healthy trapezius muscle. In the present study no differences in phosphorylation level between the corresponding individual proteins were detected using LC-MSMS and immunoblotting; instead we assigned different isoforms of regulatory MLCs. To further elucidate the contrability: calcium (Ca2+) regulatory proteins, sarco(endo)plasmic reticulum Ca2+ ATPase 1 (SERCA-1) and calsequestrine (CSQ) were analyzed by western blot. The analysis revealed a significantly increased abundance of SERCA-1 protein in the myalgic muscle and a significantly increased abundance of CSQ in healthy muscle. Myalgic muscle contraction patterns have in previous studies shown to differ from healthy muscle which may be connected to the Ca2+ availability in the muscle. Here we present the proteomic characterization of differences in Ca2+ regulating proteins and particularly regulatory MLCs in trapezius muscle of women with chronic musculoskeletal neck and shoulder pain.

Place, publisher, year, edition, pages
Proteomass Scientific Society, 2016
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:liu:diva-130539 (URN)10.5584/jiomics.v6i1.191 (DOI)
Available from: 2016-08-15 Created: 2016-08-15 Last updated: 2018-01-17
Wåhlén, K., Fornander, L., Olausson, P., Ydreborg, K., Flodin, U., Graff, P., . . . Ghafouri, B. (2016). Protein profiles of nasal lavage fluid from individuals with work-related upper airway symptoms associated to moldy and damp buildings. Indoor Air, 26(5), 743-754
Open this publication in new window or tab >>Protein profiles of nasal lavage fluid from individuals with work-related upper airway symptoms associated to moldy and damp buildings
Show others...
2016 (English)In: Indoor Air, ISSN 0905-6947, E-ISSN 1600-0668, Vol. 26, no 5, p. 743-754Article in journal (Refereed) Published
Abstract [en]

Upper airway irritation is common among individuals working in moldy and damp buildings. The aim was to investigate effects on the protein composition of the nasal lining fluid. The prevalence of symptoms in relation to work was examined in 37 individuals working in two damp buildings. Microbial growth was confirmed in one of the buildings. Nasal lavage fluid was collected from 29 exposed subjects and 13 controls. Protein profiles were investigated with a proteomic approach and evaluated by multivariate statistical models. Subjects from both workplaces reported upper airway and ocular symptoms. Based on protein profiles, symptomatic subjects in the two workplaces were discriminated from each other and separated from healthy controls. The groups differed in proteins involved in inflammation and host defense. Measurements of innate immunity proteins showed a significant increa e of protein S100-A8 and decrease of SPLUNC1 in subjects from one workplace while alpha-1-antitrypsin was elevated in subjects from the other workplace, compared to healthy controls. The results show that protein profiles in nasal lavage fluid can be used to monitor airway mucosal effects in personnel working in damp buildings and indicate that the profile may be separate when the dampness is associated with the presence of molds.

Place, publisher, year, edition, pages
Wiley-Blackwell, 2016
Keywords
Sick building syndrome, proteomics, nasal mucosa, SPLUNC1, alpha-1-antitrypsin, protein S100-A8
National Category
Occupational Health and Environmental Health Infectious Medicine
Identifiers
urn:nbn:se:liu:diva-117339 (URN)10.1111/ina.12257 (DOI)000387348500009 ()
Note

Funding agencies: Research Council of South East Sweden [FORSS-222751, FORSS-389061]; Cancer and Allergy Foundation [150441]

Available from: 2015-04-23 Created: 2015-04-23 Last updated: 2018-01-11Bibliographically approved
Christidis, N., Ghafouri, B., Larsson, A., Palstam, A., Mannerkorpi, K., Bileviciute-Ljungar, I., . . . Ernberg, M. (2015). Comparison of the Levels of Pro-Inflammatory Cytokines Released in the Vastus Lateralis Muscle of Patients with Fibromyalgia and Healthy Controls during Contractions of the Quadriceps Muscle - A Microdialysis Study. PLoS ONE, 10(12), e0143856
Open this publication in new window or tab >>Comparison of the Levels of Pro-Inflammatory Cytokines Released in the Vastus Lateralis Muscle of Patients with Fibromyalgia and Healthy Controls during Contractions of the Quadriceps Muscle - A Microdialysis Study
Show others...
2015 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 10, no 12, p. e0143856-Article in journal (Refereed) Published
Abstract [en]

Objective Fibromyalgia is associated with central hyperexcitability, but it is suggested that peripheral input is important to maintain central hyperexcitability. The primary aim was to investigate the levels of pro-inflammatory cytokines released in the vastus lateralis muscle during repetitive dynamic contractions of the quadriceps muscle in patients with fibromyalgia and healthy controls. Secondarily, to investigate if the levels of pro-inflammatory cytokines were correlated with pain or fatigue during these repetitive dynamic contractions. Material and Methods 32 women with fibromyalgia and 32 healthy women (controls) participated in a 4 hour microdialysis session, to sample IL-1 beta, IL-6, IL-8, and TNF from the most painful point of the vastus lateralis muscle before, during and after 20 minutes of repeated dynamic contractions. Pain (visual analogue scale; 0-100) and fatigue Borgs Rating of Perceived Exertion Scale; 6-20) were assessed before and during the entire microdialysis session. Results The repetitive dynamic contractions increased pain in the patients with fibromyalgia (P < .001) and induced fatigue in both groups (P < .001). Perceived fatigue was significantly higher among patients with fibromyalgia than controls (P < .001). The levels of IL-1 beta did not change during contractions in either group. The levels of TNF did not change during contractions in patients with fibromyalgia, but increased in controls (P < .001) and were significantly higher compared to patients with fibromyalgia (P = .033). The levels of IL-6 and IL-8 increased in both groups alike during and after contractions (Ps < .001). There were no correlations between pain or fatigue and cytokine levels after contractions. Conclusion There were no differences between patients with fibromyalgia and controls in release of pro-inflammatory cytokines, and no correlations between levels of pro-inflammatory cytokines and pain or fatigue. Thus, this study indicates that IL-1 beta, IL-6, IL-8, and TNF do not seem to play an important role in maintenance of muscle pain in fibromyalgia.

Place, publisher, year, edition, pages
PUBLIC LIBRARY SCIENCE, 2015
National Category
Basic Medicine
Identifiers
urn:nbn:se:liu:diva-123776 (URN)10.1371/journal.pone.0143856 (DOI)000365891600058 ()26624891 (PubMedID)
Note

Funding Agencies|Swedish Rheumatism Association; Swedish Research Council; Health and Medical Care Executive Board of Vastra Gotaland Region; ALF-LUA at Sahlgrenska University Hospital; Linkoping University Hospital, Linkoping University; Gothenburg Center for Person Centered Care (GPCC); AFA Insurance; ALF-Stockholm County Council; Karolinska Institute Foundation

Available from: 2016-01-11 Created: 2016-01-11 Last updated: 2018-01-10
Shimada, A., Baad-Hansen, L., Castrillon, E., Ghafouri, B., Stensson, N., Gerdle, B., . . . Svensson Odont, P. (2015). Differential effects of repetitive oral administration of monosodium glutamate on interstitial glutamate concentration and muscle pain sensitivity. Nutrition (Burbank, Los Angeles County, Calif.), 31(2), 315-323
Open this publication in new window or tab >>Differential effects of repetitive oral administration of monosodium glutamate on interstitial glutamate concentration and muscle pain sensitivity
Show others...
2015 (English)In: Nutrition (Burbank, Los Angeles County, Calif.), ISSN 0899-9007, E-ISSN 1873-1244, Vol. 31, no 2, p. 315-323Article in journal (Refereed) Published
Abstract [en]

Objective: The aim of this study was to determine the relationship of high daily monosodium glutamate (MSG) consumption with glutamate concentrations in jaw muscle, saliva, and serum, and muscle pain sensitivity in healthy participants. Methods: A randomized, double-blinded, placebo-controlled study was conducted to investigate the effect of repetitive consumption of high-dose MSG on glutamate concentration in the masseter muscles measured by microdialysis and muscle pain sensitivity. In five contiguous experimental daily sessions, 32 healthy participants drank MSG (150 mg/kg) or NaCl (24 mg/kg) diluted with a 400 mL soda. The concentrations of glutamate before and after the ingestion were assessed in dialysate and plasma samples on the first and last days. Saliva glutamate concentration was assessed every day. Pressure pain threshold, pressure pain tolerance, autonomic parameters (heart rate, systolic and diastolic blood pressures) and reported side effects also were assessed. Results: No significant change was noted in the baseline concentration of glutamate in the masseter muscle, blood, or saliva, but the peak concentration in the masseter muscle increased significantly between day 1 and 5. A statistically significant increase in systolic and diastolic blood pressures after MSG administration was observed, as well as a significantly higher frequency of reports of nausea and headache in the MSG group. No robust effect of MSG on muscle sensitivity was found. Conclusion: Interstitial glutamate concentration in the masseter muscle is not highly disturbed by excessive repetitive intake of MSG in healthy man. (C) 2015 Elsevier Inc. All rights reserved.

Place, publisher, year, edition, pages
Elsevier, 2015
Keywords
Microdialysis; Mechanical muscle sensitivity; Monosodium glutamate; Myofascial temporomandibular disorders; Diet; Pain
National Category
Basic Medicine
Identifiers
urn:nbn:se:liu:diva-114991 (URN)10.1016/j.nut.2014.07.011 (DOI)000348633400008 ()25592010 (PubMedID)
Note

Funding Agencies|Danish Dental Association; Danish Medical Research Council

Available from: 2015-03-09 Created: 2015-03-06 Last updated: 2018-01-11
Organisations

Search in DiVA

Show all publications