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Mandenius, Carl-FredrikORCID iD iconorcid.org/0000-0001-9711-794x
Alternative names
Publications (10 of 96) Show all publications
Christoffersson, J., Aronsson, C., Jury, M., Selegård, R., Aili, D. & Mandenius, C.-F. (2019). Fabrication of modular hyaluronan-PEG hydrogels to support 3D cultures of hepatocytes in a perfused liver-on-a-chip device. Biofabrication, 11(1), Article ID 015013.
Open this publication in new window or tab >>Fabrication of modular hyaluronan-PEG hydrogels to support 3D cultures of hepatocytes in a perfused liver-on-a-chip device
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2019 (English)In: Biofabrication, ISSN 1758-5082, E-ISSN 1758-5090, Vol. 11, no 1, article id 015013Article in journal (Refereed) Published
Abstract [en]

Liver cell culture models are attractive in both tissue engineering and for development of assays for drug toxicology research. To retain liver specific cell functions, the use of adequate cell types and culture conditions, such as a 3Dorientation of the cells and a proper supply of nutrients and oxygen, are critical. In this article, we show how extracellular matrix mimetic hydrogels can support hepatocyte viability and functionality in a perfused liver-on-a-chip device. A modular hydrogel system based on hyaluronan and poly(ethylene glycol) (HA-PEG), modified with cyclooctyne moieties for bioorthogonal strain-promoted alkyne-azide 1, 3-dipolar cycloaddition (SPAAC), was developed, characterized, and compared for cell compatibility to hydrogels based on agarose and alginate. Hepatoma cells (HepG2) formed spheroids with viable cells in all hydrogels with the highest expression of albumin and urea in alginate hydrogels. By including an excess of cyclooctyne in theHA backbone, azide-modified cell adhesion motifs (linear and cyclicRGDpeptides) could be introduced in order to enhance viability and functionality of human induced pluripotent stem cell derived hepatocytes (hiPS-HEPs). In the HA-PEG hydrogels modified with cyclicRGDpeptides hiPS-HEPs migrated and grew in 3D and showed an increased viability and higher albumin production compared to when cultured in the other hydrogels. This flexible SPAAC crosslinked hydrogel system enabled fabrication of perfused 3D cell culture of hiPS-HEPs and is a promising material for further development and optimization of liver-on-a-chip devices.

Place, publisher, year, edition, pages
Institute of Physics Publishing (IOPP), 2019
Keywords
Organ-on-a-chip; biofabrication; bioorthogonal crosslinking; cell-binding motif; microfluidics
National Category
Cell and Molecular Biology
Identifiers
urn:nbn:se:liu:diva-153971 (URN)10.1088/1758-5090/aaf657 (DOI)000454550900002 ()30523863 (PubMedID)2-s2.0-85059228017 (Scopus ID)
Note

Funding Agencies|EU Innovative Medicines Initiative Joint Undertaking [115439]; European Union; Swedish Research Council (VR) [B0431901]; Swedish Foundation for Strategic Research (SFF) [FFL15-0026]; Carl Trygger Foundation [CTS15:79]; Knut and Alice Wallenberg Foundation [KAW 2016.0231]; Swedish Government Strategic Research Area in Materials Science on Functional Materials at Linkoping University (Faculty Grant SFO-Mat-LiU) [2009-00971]

Available from: 2019-01-22 Created: 2019-01-22 Last updated: 2019-02-01Bibliographically approved
Christoffersson, J., Meier, F., Kempf, H., Schwanke, K., Coffee, M., Beilmann, M., . . . Mandenius, C.-F. (2018). A Cardiac Cell Outgrowth Assay for Evaluating Drug Compounds Using a Cardiac Spheroid-on-a-Chip Device. Bioengineering, 5(2), 1-13, Article ID 36.
Open this publication in new window or tab >>A Cardiac Cell Outgrowth Assay for Evaluating Drug Compounds Using a Cardiac Spheroid-on-a-Chip Device
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2018 (English)In: Bioengineering, E-ISSN 2306-5354, Vol. 5, no 2, p. 1-13, article id 36Article in journal (Refereed) Published
Abstract [en]

Three-dimensional (3D) models with cells arranged in clusters or spheroids have emerged as valuable tools to improve physiological relevance in drug screening. One of the challenges with cells cultured in 3D, especially for high-throughput applications, is to quickly and non-invasively assess the cellular state in vitro. In this article, we show that the number of cells growing out from human induced pluripotent stem cell (hiPSC)-derived cardiac spheroids can be quantified to serve as an indicator of a drug’s effect on spheroids captured in a microfluidic device. Combining this spheroid-on-a-chip with confocal high content imaging reveals easily accessible, quantitative outgrowth data. We found that effects on outgrowing cell numbers correlate to the concentrations of relevant pharmacological compounds and could thus serve as a practical readout to monitor drug effects. Here, we demonstrate the potential of this semi-high-throughput “cardiac cell outgrowth assay” with six compounds at three concentrations applied to spheroids for 48 h. The image-based readout complements end-point assays or may be used as a non-invasive assay for quality control during long-term culture.

National Category
Cell and Molecular Biology Biomedical Laboratory Science/Technology Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
urn:nbn:se:liu:diva-154007 (URN)10.3390/bioengineering5020036 (DOI)
Available from: 2019-01-22 Created: 2019-01-22 Last updated: 2019-03-29Bibliographically approved
Bengtsson, K., Christoffersson, J., Mandenius, C.-F. & Robinson, N. D. (2018). A clip-on electroosmotic pump for oscillating flow in microfluidic cell culture devices. Microfluidics and Nanofluidics, 22(3), Article ID 27.
Open this publication in new window or tab >>A clip-on electroosmotic pump for oscillating flow in microfluidic cell culture devices
2018 (English)In: Microfluidics and Nanofluidics, ISSN 1613-4982, E-ISSN 1613-4990, Vol. 22, no 3, article id 27Article in journal (Refereed) Published
Abstract [en]

Recent advances in microfluidic devices put a high demand on small, robust and reliable pumps suitable for high-throughput applications. Here we demonstrate a compact, low-cost, directly attachable (clip-on) electroosmotic pump that couples with standard Luer connectors on a microfluidic device. The pump is easy to make and consists of a porous polycarbonate membrane and poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) electrodes. The soft electrode and membrane materials make it possible to incorporate the pump into a standard syringe filter holder, which in turn can be attached to commercial chips. The pump is less than half the size of the microscope slide used for many commercial lab-on-a-chip devices, meaning that these pumps can be used to control fluid flow in individual reactors in highly parallelized chemistry and biology experiments. Flow rates at various electric current and device dimensions are reported. We demonstrate the feasibility and safety of the pump for biological experiments by exposing endothelial cells to oscillating shear stress (up to 5 dyn/cm2) and by controlling the movement of both micro- and macroparticles, generating steady or oscillatory flow rates up to ± 400 μL/min.

Place, publisher, year, edition, pages
Springer Berlin/Heidelberg, 2018
National Category
Other Medical Biotechnology
Identifiers
urn:nbn:se:liu:diva-145301 (URN)10.1007/s10404-018-2046-4 (DOI)000427527600005 ()
Note

Funding agencies: Swedish Research Council (Vetenskapsradet) [2015-03298]

Available from: 2018-02-21 Created: 2018-02-21 Last updated: 2019-01-22Bibliographically approved
Christoffersson, J., Aronsson, C., Jury, M., Selegård, R., Aili, D. & Mandenius, C.-F. (2018). Fabrication of modular hyaluronan-PEG hydrogels to support 3D cultures of hepatocytes in a perfused liver-on-a-chip device. Biofabrication, 11(1), 1-13, Article ID 015013.
Open this publication in new window or tab >>Fabrication of modular hyaluronan-PEG hydrogels to support 3D cultures of hepatocytes in a perfused liver-on-a-chip device
Show others...
2018 (English)In: Biofabrication, ISSN 1758-5082, E-ISSN 1758-5090, Vol. 11, no 1, p. 1-13, article id 015013Article in journal (Refereed) Published
Abstract [en]

Liver cell culture models are attractive in both tissue engineering and for development of assays for drug toxicology research. To retain liver specific cell functions, the use of adequate cell types and culture conditions, such as a 3D orientation of the cells and a proper supply of nutrients and oxygen, are critical. In this article, we show how extracellular matrix mimetic hydrogels can support hepatocyte viability and functionality in a perfused liver-on-a-chip device. A modular hydrogel system based on hyaluronan and poly(ethylene glycol) (HA-PEG), modified with cyclooctyne moieties for bioorthogonal strain-promoted alkyne-azide 1, 3-dipolar cycloaddition (SPAAC), was developed, characterized, and compared for cell compatibility to hydrogels based on agarose and alginate. Hepatoma cells (HepG2) formed spheroids with viable cells in all hydrogels with the highest expression of albumin and urea in alginate hydrogels. By including an excess of cyclooctyne in the HA backbone, azide-modified cell adhesion motifs (linear and cyclic RGD peptides) could be introduced in order to enhance viability and functionality of human induced pluripotent stem cell derived hepatocytes (hiPS-HEPs). In the HA-PEG hydrogels modified with cyclic RGD peptides hiPS-HEPs migrated and grew in 3D and showed an increased viability and higher albumin production compared to when cultured in the other hydrogels. This flexible SPAAC crosslinked hydrogel system enabled fabrication of perfused 3D cell culture of hiPS-HEPs and is a promising material for further development and optimization of liver-on-a-chip devices.

Place, publisher, year, edition, pages
Institute of Physics (IOP), 2018
National Category
Cell and Molecular Biology Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy) Cell Biology
Identifiers
urn:nbn:se:liu:diva-154008 (URN)10.1088/1758-5090/aaf657 (DOI)
Available from: 2019-01-22 Created: 2019-01-22 Last updated: 2019-01-22Bibliographically approved
Mandenius, C.-F. (Ed.). (2016). Bioreactors : design, operation and novel applications. Weinheim: Wiley-VCH
Open this publication in new window or tab >>Bioreactors : design, operation and novel applications
2016 (English)Collection (editor) (Other academic)
Abstract [en]

In this expert handbook both the topics and contributors are selected so as to provide an authoritative view of possible applications for this new technology. The result is an up-to-date survey of current challenges and opportunities in the design and operation of bioreactors for high-value products in the biomedical and chemical industries.

Combining theory and practice, the authors explain such leading-edge technologies as single-use bioreactors, bioreactor simulators, and soft sensor monitoring, and discuss novel applications, such as stem cell production, process development, and multi-product reactors, using case studies from academia as well as from industry. A final section addresses the latest trends, including culture media design and systems biotechnology, which are expected to have an increasing impact on bioreactor design.

With its focus on cutting-edge technologies and discussions of future developments, this handbook will remain an invaluable reference for many years to come.

Place, publisher, year, edition, pages
Weinheim: Wiley-VCH, 2016. p. 520
Keywords
Bioteknik
National Category
Industrial Biotechnology Production Engineering, Human Work Science and Ergonomics
Identifiers
urn:nbn:se:liu:diva-129318 (URN)978-35-2733-768-2 (ISBN)
Available from: 2016-06-16 Created: 2016-06-16 Last updated: 2019-01-22Bibliographically approved
Mandenius, C.-F. (2016). Challenges for bioreactor design and operation. In: Carl-Fredrik Mandenius (Ed.), Bioreactors: design, operation and novel applications (pp. 1-34). Weinheim: Wiley-VCH Verlagsgesellschaft
Open this publication in new window or tab >>Challenges for bioreactor design and operation
2016 (English)In: Bioreactors: design, operation and novel applications / [ed] Carl-Fredrik Mandenius, Weinheim: Wiley-VCH Verlagsgesellschaft, 2016, p. 1-34Chapter in book (Other academic)
Place, publisher, year, edition, pages
Weinheim: Wiley-VCH Verlagsgesellschaft, 2016
Keywords
Bioteknik
National Category
Industrial Biotechnology Production Engineering, Human Work Science and Ergonomics
Identifiers
urn:nbn:se:liu:diva-129319 (URN)978-35-2733-768-2 (ISBN)
Available from: 2016-06-16 Created: 2016-06-16 Last updated: 2019-01-22Bibliographically approved
Mandenius, C.-F. (2016). Design-of-experiments for development and optimization of bioreactor media. In: Carl-Fredrik Mandenius (Ed.), Bioreactors: design, operation and novel applications (pp. 421-451). Weinheim: Wiley-VCH Verlagsgesellschaft
Open this publication in new window or tab >>Design-of-experiments for development and optimization of bioreactor media
2016 (English)In: Bioreactors: design, operation and novel applications / [ed] Carl-Fredrik Mandenius, Weinheim: Wiley-VCH Verlagsgesellschaft, 2016, p. 421-451Chapter in book (Other academic)
Place, publisher, year, edition, pages
Weinheim: Wiley-VCH Verlagsgesellschaft, 2016
Keywords
Bioteknik
National Category
Industrial Biotechnology Production Engineering, Human Work Science and Ergonomics
Identifiers
urn:nbn:se:liu:diva-129320 (URN)978-35-2733-768-2 (ISBN)
Available from: 2016-06-16 Created: 2016-06-16 Last updated: 2019-01-22Bibliographically approved
Mandenius, C.-F. & Gustavsson, R. (2016). Soft sensor design for bioreactor monitoring and control. In: Carl-Fredrik Mandenius (Ed.), Bioreactors: design, operation and novel application (pp. 391-420). Weinheim: Wiley-VCH Verlagsgesellschaft
Open this publication in new window or tab >>Soft sensor design for bioreactor monitoring and control
2016 (English)In: Bioreactors: design, operation and novel application / [ed] Carl-Fredrik Mandenius, Weinheim: Wiley-VCH Verlagsgesellschaft, 2016, p. 391-420Chapter in book (Other academic)
Place, publisher, year, edition, pages
Weinheim: Wiley-VCH Verlagsgesellschaft, 2016
Keywords
Bioteknik
National Category
Industrial Biotechnology Production Engineering, Human Work Science and Ergonomics
Identifiers
urn:nbn:se:liu:diva-129321 (URN)978-35-2733-768-2 (ISBN)
Available from: 2016-06-16 Created: 2016-06-16 Last updated: 2019-01-22Bibliographically approved
Johansson, L. B. G., Simon, R., Bergström, G., Eriksson, M., Prokop, S., Mandenius, C.-F., . . . Nilsson, P. (2015). An azide functionalized oligothiophene ligand - A versatile tool for multimodal detection of disease associated protein aggregates. Biosensors & bioelectronics, 63, 204-211
Open this publication in new window or tab >>An azide functionalized oligothiophene ligand - A versatile tool for multimodal detection of disease associated protein aggregates
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2015 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 63, p. 204-211Article in journal (Refereed) Published
Abstract [en]

Ligands for identifying protein aggregates are of great interest as such deposits are the pathological hallmark of a wide range of severe diseases including Alzheimers and Parkinsons disease. Here we report the synthesis of an azide functionalized fluorescent pentameric oligothiophene that can be utilized as a ligand for multimodal detection of disease-associated protein aggregates. The azide functionalization allows for attachment of the ligand to a surface by conventional click chemistry without disturbing selective interaction with protein aggregates and the oligothiophene-aggregate interaction can be detected by fluorescence or surface plasmon resonance. In addition, a methodology where the oligothiophene ligand is employed as a capturing molecule selective for aggregated proteins in combination with an antibody detecting a distinct peptide/protein is also presented. We foresee that this methodology will offer the possibility to create a variety of multiplex sensing systems for sensitive and selective detection of protein aggregates, the pathological hallmarks of several neurodegenerative diseases.

Place, publisher, year, edition, pages
Elsevier, 2015
Keywords
Protein aggregates; Oligothiophene; Fluorescence; Surface plasmon resonance; Click chemistry
National Category
Chemical Sciences Biological Sciences
Identifiers
urn:nbn:se:liu:diva-112169 (URN)10.1016/j.bios.2014.07.042 (DOI)000343337000030 ()25089818 (PubMedID)
Note

Funding Agencies|Swedish Foundation for Strategic Research; Ehrling Persson Foundation; ERC Starting Independent Researcher grant (Project: MUMID)

Available from: 2014-11-18 Created: 2014-11-18 Last updated: 2019-01-22
Bergström, G., Kuusk, A. & Mandenius, C.-F. (2015). Capacitive biosensor for detection of toxicity biomarkers.
Open this publication in new window or tab >>Capacitive biosensor for detection of toxicity biomarkers
2015 (English)Manuscript (preprint) (Other academic)
Abstract [en]

Microfluidic devices are rapidly gaining importance for in vitro toxicity testing. Biomarker detection in microfluidic assays are however challenging due to small sample sizes and low analyte concentration. Capacitive electrochemical biosensors have been reported to have high sensitivity and properties that are amenable for implementation into microfluidic devices.

In this work a biosensor application for troponin T (TnT) is presented. The sensor showed linear response to analyte over five orders of magnitude with the lowest detectable signal at 10-13 M. The sensor proved to be able to detect TnT spiked in cell culture media at concentrations relevant for cell cultures.

National Category
Biological Sciences Physical Sciences
Identifiers
urn:nbn:se:liu:diva-118293 (URN)
Available from: 2015-05-26 Created: 2015-05-26 Last updated: 2019-01-22
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0001-9711-794x

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