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2024 (English)In: The Analyst, ISSN 0003-2654, E-ISSN 1364-5528, Vol. 149, no 17, p. 4496-4505Article in journal (Refereed) Published
Abstract [en]
Polyploid giant cancer cells (PGCCs) contribute to the genetic heterogeneity and evolutionary dynamics of tumors. Their size, however, complicates their isolation from mainstream tumor cell populations. Standard techniques like fluorescence-activated cell sorting (FACS) rely on fluorescent labeling, introducing potential challenges in subsequent PGCC analyses. In response, we developed the Isosceles Trapezoidal Spiral Microchannel (ITS mu C), a microfluidic device optimizing the Dean drag force (FD) and exploiting uniform vortices for enhanced separation. Numerical simulations highlighted ITS mu C's advantage in producing robust FD compared to rectangular and standard trapezoidal channels. Empirical results confirmed its ability to segregate larger polystyrene (PS) particles (avg. diameter: 50 mu m) toward the inner wall, while directing smaller ones (avg. diameter: 23 mu m) outward. Utilizing ITS mu C, we efficiently isolated PGCCs from doxorubicin-resistant triple-negative breast cancer (DOXR-TNBC) and patient-derived cancer (PDC) cells, achieving outstanding purity, yield, and viability rates (all greater than 90%). This precision was accomplished without fluorescent markers, and the versatility of ITS mu C suggests its potential in differentiating a wide range of heterogeneous cell populations. Polyploid giant cancer cells (PGCCs) contribute to the genetic heterogeneity and evolutionary dynamics of tumors.
Place, publisher, year, edition, pages
ROYAL SOC CHEMISTRY, 2024
National Category
Biomedical Laboratory Science/Technology
Identifiers
urn:nbn:se:liu:diva-206321 (URN)10.1039/d4an00750f (DOI)001275477100001 ()39049608 (PubMedID)
Note
Funding Agencies|Korea-Sweden Cooperation Program through STINT (the Swedish Foundation for International Cooperation in Research and Higher Education); National Research Foundation (NRF) through the Ministry of Science and ICT (MSIT) of Korea [RS-2023-00218543, RS-2023-00242443]; National Research Council of Science & Technology (NST) [CRC22021-200]
2024-08-152024-08-152025-02-18Bibliographically approved