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Kihlström, Erik
Publications (10 of 23) Show all publications
Fornander, L., Ghafouri, B., Kihlström, E., Åkerlind, B., Schön, T., Tagesson, C. & Lindahl, M. (2011). Innate immunity proteins and a new truncated form of SPLUNC1 in nasopharyngeal aspirates from infants with respiratory syncytial virus infection. PROTEOMICS CLINICAL APPLICATIONS, 5(9-10), 513-522
Open this publication in new window or tab >>Innate immunity proteins and a new truncated form of SPLUNC1 in nasopharyngeal aspirates from infants with respiratory syncytial virus infection
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2011 (English)In: PROTEOMICS CLINICAL APPLICATIONS, ISSN 1862-8346, Vol. 5, no 9-10, p. 513-522Article in journal (Refereed) Published
Abstract [en]

Purpose: Respiratory syncytial virus (RSV) is the most common cause of severe respiratory tract infection in infants. The aim was to identify host defence components in nasopharyngeal aspirate (NPA) from infants with RSV infection and to study the expression of the novel 25 kDa innate immunity protein SPLUNC1. less thanbrgreater than less thanbrgreater thanExperimental design: NPAs from infants were analyzed with 2-DE and MS in a pilot study. The levels of SPLUNC1 were analyzed with immunoblotting in 47 NPAs, admitted for RSV diagnosis. less thanbrgreater than less thanbrgreater thanResults: Totally, 35 proteins were identified in NPA, including several innate immunity proteins such as group X phospholipase A(2), different S100 proteins and SPLUNC1. In addition, a new truncated 15 kDa form of SPLUNC1 was identified that was detected in about 50% of the aspirates admitted for RSV diagnosis. RSV-positive boys had significantly less 25 kDa SPLUNC1 than RSV-negative boys while there were no significant differences among girls. less thanbrgreater than less thanbrgreater thanConclusions and clinical relevance: Several important innate immunity proteins were identified in NPA. Notably, a new truncated form of the newly suggested anti-bacterial protein SPLUNC1 was found. It is possible that a decrease in SPLUNC1 in the upper airways may increase the risk for severe pneumonia in boys.

Place, publisher, year, edition, pages
Wiley-VCH Verlag Berlin, 2011
Keywords
MS, Nasopharynx, PLUNC, Respiratory syncytial virus, Two-dimensional gel electrophoresis
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-72142 (URN)10.1002/prca.201100016 (DOI)000296418400005 ()
Note
Funding Agencies|The Research Council of South East Sweden|FORSS-36761- 8505|Available from: 2011-11-18 Created: 2011-11-18 Last updated: 2015-04-23
Friberg, O., Dahlin, L.-G., Kallman, J., Kihlström, E., Soderquist, B. & Svedjeholm, R. (2009). COLLAGEN-GENTAMICIN IMPLANT FOR PREVENTION OF STERNAL WOUND INFECTION; LONG TERM EFFECTIVENESS. In: in INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS vol 33 (pp. S42-S42). , 33
Open this publication in new window or tab >>COLLAGEN-GENTAMICIN IMPLANT FOR PREVENTION OF STERNAL WOUND INFECTION; LONG TERM EFFECTIVENESS
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2009 (English)In: in INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS vol 33, 2009, Vol. 33, p. S42-S42Conference paper, Published paper (Refereed)
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-19402 (URN)
Available from: 2009-06-23 Created: 2009-06-22 Last updated: 2009-08-18
Friberg, Ö., Dahlin, L.-G., Källman, J., Kihlström, E., Söderquist, B. & Svedjeholm, R. (2009). Collagen-gentamicin implant for prevention of sternal wound infection; long-term follow-up of effectiveness. Interactive Cardiovascular and Thoracic Surgery, 9(3), 454-458
Open this publication in new window or tab >>Collagen-gentamicin implant for prevention of sternal wound infection; long-term follow-up of effectiveness
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2009 (English)In: Interactive Cardiovascular and Thoracic Surgery, ISSN 1569-9293, E-ISSN 1569-9285, Vol. 9, no 3, p. 454-458Article in journal (Refereed) Published
Abstract [en]

In a previous randomized controlled trial (LOGIP trial) the addition of local collagen-gentamicin reduced the incidence of postoperative sternal wound infections (SWI) compared with intravenous prophylaxis only. Consequently, the technique with local gentamicin was introduced in clinical routine at the two participating centers. The aim of the present study was to re-evaluate the technique regarding the prophylactic effect against SWI and to detect potential shifts in causative microbiological agents over time. All patients in this prospective two-center study received prophylaxis with application of two collagen-gentamicin sponges between the sternal halves in addition to routine intravenous antibiotics. All patients were followed for 60 days postoperatively. From January 2007 to May 2008, 1359 patients were included. The 60-day incidences of any SWI was 3.7% and of deep SWI 1.5% (1.0% mediastinitis). Both superficial and deep SWI were significantly reduced compared with the previous control group (OR=0.34 for deep SWI, Pless than0.001). There was no increase in the absolute incidence of aminoglycoside resistant agents. The majority of SWI were caused by coagulase-negative staphylococci (CoNS). The incidence of deep SWI caused by Staphylococcus aureus was 0.07%. The results indicate a maintained effect of the prophylaxis over time without absolute increase in aminoglycoside resistance.

Place, publisher, year, edition, pages
Oxford University Press, 2009
Keywords
Antibiotics; Cardiac surgery; Complications; Mediastinitis; Regression analysis; Risk factors; Statistics; Wound infection
National Category
Surgery
Identifiers
urn:nbn:se:liu:diva-21243 (URN)10.1510/icvts.2009.207514 (DOI)19541691 (PubMedID)
Available from: 2009-09-30 Created: 2009-09-30 Last updated: 2017-12-13Bibliographically approved
Stark, L., Matussek, A., Strindhall, J., Geffers, R., Buer, J., Kihlström, E., . . . Lindgren, P.-E. (2009). Staphylococcus aureus isolates from blood and anterior nares induce similar innate immune responses in endothelial cells. APMIS, 117(11), 814-824
Open this publication in new window or tab >>Staphylococcus aureus isolates from blood and anterior nares induce similar innate immune responses in endothelial cells
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2009 (English)In: APMIS, ISSN 0903-4641, Vol. 117, no 11, p. 814-824Article in journal (Refereed) Published
Abstract [en]

To evaluate the possibility to distinguish virulent from non-virulent isolates, gene expression in human umbilical vein endothelial cells (HUVEC) induced by invasive and colonizing isolates of Staphylococcus aureus was compared. Gene expression in HUVEC was analyzed by microarray analysis after 4 h of infection with Staphylococcus aureus, isolated from healthy nasal carriers (n = 5) and from blood of septic patients (n = 5), to explore possible differences between the groups of bacteria in interaction with HUVEC. All isolates were spa-typed to disclose strain relatedness. Moreover, the isolates were characterized with DNA microarray to determine the presence of virulence genes and to investigate the potential genes of importance in HUVEC interaction. The expression of 41 genes was up-regulated, and four were down-regulated in HUVEC by all isolates. Most of the up-regulated genes encode cytokines, chemokines, interferon-induced proteins, proteins regulating apoptosis and cell proliferation. There was no difference in the gene expression pattern between HUVEC infected with invasive or colonizing isolates. Furthermore, there was no difference in the presence of bacterial virulence genes between the two groups. In conclusion, our data indicate that S. aureus isolates induce comparable expression patterns in HUVEC, irrespective of invasiveness or presence of virulence genes.

Keywords
Staphylococcus aureus, HUVEC, infection, gene expression, virulence
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-51487 (URN)10.1111/j.1600-0463.2009.02535.x (DOI)
Available from: 2009-11-04 Created: 2009-11-04 Last updated: 2014-01-16Bibliographically approved
Kälvegren, H., Fridfeldt (Berggren), J., Garvin, P., Wind, L., Leanderson, P., Kristenson, M., . . . Richter, A. (2008). Correlation between rises in Chlamydia pneumoniae-specific antibodies, platelet activation and lipid peroxidation after percutaneous coronary intervention.. European Journal of Clinical Microbiology and Infectious Diseases, 27(7), 503-511
Open this publication in new window or tab >>Correlation between rises in Chlamydia pneumoniae-specific antibodies, platelet activation and lipid peroxidation after percutaneous coronary intervention.
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2008 (English)In: European Journal of Clinical Microbiology and Infectious Diseases, ISSN 0934-9723, E-ISSN 1435-4373, Vol. 27, no 7, p. 503-511Article in journal (Refereed) Published
Abstract [en]

We recently showed that Chlamydia pneumoniae activates platelets in vitro, with an associated oxidation of low-density lipoproteins. The aim of this study was to investigate whether C. pneumoniae is released during percutaneous coronary intervention (PCI) and, thereby, causes platelet activation and lipid peroxidation. Seventy-three patients undergoing coronary angiography and following PCI or coronary artery bypass graft (CABG) and 57 controls were included in the study. C. pneumoniae antibodies, serotonin and lipid peroxidation were measured before and 24 h, 1 month and 6 months after angiography. The results show that serum C. pneumoniae IgA concentrations were significantly higher in patients than in the controls. Furthermore, in 38% of the C. pneumoniae IgG positive patients, the C. pneumoniae IgG concentration increased 1 month after PCI. The levels of C. pneumoniae IgG antibodies 1 month after PCI correlated with plasma-lipid peroxidation (r = 0.91, P < 0.0001) and platelet-derived serotonin (r = 0.62, P = 0.02). There was no elevation in the total serum IgG 1 month after PCI. In conclusion, the present results suggest that PCI treatment of coronary stenosis releases C. pneumoniae from the atherosclerotic lesions, which leads to platelet activation and lipid peroxidation.

National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-14388 (URN)10.1007/s10096-008-0465-y (DOI)
Available from: 2007-04-20 Created: 2007-04-20 Last updated: 2017-12-13Bibliographically approved
Högdahl, M., Söderlund, G. & Kihlström, E. (2008). Expression of chemokines and adhesion molecules in human coronary artery endothelial cells infected with Chlamydia (Chlamydophila) pneumoniae. APMIS, 116(12), 1082-1088
Open this publication in new window or tab >>Expression of chemokines and adhesion molecules in human coronary artery endothelial cells infected with Chlamydia (Chlamydophila) pneumoniae
2008 (English)In: APMIS, ISSN 0903-4641, Vol. 116, no 12, p. 1082-1088Article in journal (Refereed) Published
Abstract [en]

Chlamydia pneumoniae has during recent years been associated with cardiovascular disease and atherosclerosis. Chemokines, leukocyte adhesion proteins and metalloproteinases are significant for chemotaxis and attachment of leukocytes to vessel walls, and for stability of atherosclerotic plaques. To determine the ability of C. pneumoniae to elicit inflammation in a relevant target host cell, we infected human coronary artery endothelial cells (HCAEC) with a clinical isolate of C. pneumoniae. Extracellular release of five chemokines, two adhesion proteins and a metalloproteinase was measured at different time points after infection using a cytometric bead assay and ELISA. Secretion of IL-8, MCP-1, MIG, IP-10 and ICAM-1 was significantly increased 48 h after C. pneumoniae infection of HCAEC in comparison with uninfected controls. Release of RANTES occurred already 6 h after infection. C. pneumoniae did not elicit release of E-selectin or MMP-1. We conclude that C. pneumoniae induces expression of proinflammatory components in HCAEC, which would promote migration of leukocytes towards endothelial cells. This suggests that C. pneumoniae initiates and propagates vascular inflammation in ways that contribute to coronary artery disease.

Keywords
Coronary artery inflammation, C, pneumoniae, chemokines
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-16422 (URN)10.1111/j.1600-0463.2008.01145.x (DOI)
Note
The definitive version is available at www.blackwell-synergy.com:Marie Högdahl, G Söderlund and Erik Kihlström, Expression of chemokines and adhesion molecules in human coronary artery endothelial cells infected with Chlamydia (Chlamydophila) pneumoniae, 2008, APMIS, (116), 12, 1082-1088.http://dx.doi.org/10.1111/j.1600-0463.2008.01145.xCopyright: Blackwell Publishing Ltdhttp://www.blackwellpublishing.com/Available from: 2009-03-06 Created: 2009-01-23 Last updated: 2009-08-19Bibliographically approved
Högdahl, M. & Kihlström, E. (2007). Leucocyte esterase testing of first-voided urine and urethral and cervical smears to identify Mycoplasma genitalium-infected men and women.. International Journal of STD and AIDS (London), 18(12), 835-838
Open this publication in new window or tab >>Leucocyte esterase testing of first-voided urine and urethral and cervical smears to identify Mycoplasma genitalium-infected men and women.
2007 (English)In: International Journal of STD and AIDS (London), ISSN 0956-4624, E-ISSN 1758-1052, Vol. 18, no 12, p. 835-838Article in journal (Refereed) Published
Abstract [en]

Leucocyte esterase (LE) in first-voided urine (FVU) and presence of leucocytes in urethral and cervical smears were evaluated to identify Mycoplasma genitalium infection in 416 men and 417 women attending Department of Genitourinary Medicine. M. genitalium was diagnosed in FVU specimens by realtime polymerase chain reaction. The prevalence of M. genitalium was 6.5% in women and 6.7% in men. In total, 88.5% (23/26) of M. genitalium-infected men were identified by a combination of urethral smear and the LE test. In women, the combination of urethral and/or cervical smears and/or a positive LE test identified 91.3% (21/23) of M. genitalium-infected patients. Organism load in FVU correlated significantly with presence of urethritis (> or =4 leucocytes per high-power field) in men. A combination of LE testing of urine and urethral and/or cervical smears can be used as screening tests to select patients for specific M. genitalium testing. By this strategy, about 10% of infected individuals will remain undetected.

Keywords
Mycoplasma genitalium, leucocyte esterase, real-time PCR
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-17127 (URN)10.1258/095646207782716983 (DOI)18073017 (PubMedID)
Available from: 2009-03-06 Created: 2009-03-06 Last updated: 2017-12-13
Schöier, J., Högdahl, M., Söderlund, G. & Kihlström, E. (2006). Chlamydia (Chlamydophila) pneumoniae-induced cell death in human coronary artery endothelial cells is caspase-independent and accompanied by subcellular translocations of Bax and apoptosis-inducing factor.. FEMS Immunology and Medical Microbiology, 47(2), 207-216
Open this publication in new window or tab >>Chlamydia (Chlamydophila) pneumoniae-induced cell death in human coronary artery endothelial cells is caspase-independent and accompanied by subcellular translocations of Bax and apoptosis-inducing factor.
2006 (English)In: FEMS Immunology and Medical Microbiology, ISSN 0928-8244, E-ISSN 1574-695X, Vol. 47, no 2, p. 207-216Article in journal (Refereed) Published
Abstract [en]

Atherosclerosis and coronary heart disease are causing high morbidity and mortality worldwide. Different risk factors have been demonstrated, but the exact mechanisms behind these diseases are still not fully understood. Recent studies have suggested Chlamydia pneumoniae to be involved in the pathogenesis, and increased apoptotic indexes in atherosclerotic plaques have been documented. In this study, we show that C. pneumoniae induces apoptosis and necrosis in populations of human coronary artery endothelial cells. Apoptosis was determined by TUNEL and flow cytometry after staining of cells with annexin V and propidium iodide, and defined as TUNEL-reactive or annexin V-positive, propidium iodide-negative cells. The apoptosis was induced within 2 h postinfection and increased with inoculation dose. The general caspase inhibitor z-VAD-fmk did not affect apoptotic frequencies. By immunochemistry and immunoblot, we demonstrated activation and subcellular translocation of the proapoptotic protein Bax, and translocation of apoptosis-inducing factor from the cytosol to the nucleus. These results indicate that C. pneumoniae-induced apoptosis in human coronary artery endothelial cells is caspase-independent and regulated by Bax and apoptosis-inducing factor.

Keywords
Chlamydia pneumoniae, coronary cells, apoptosis
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-17128 (URN)10.1111/j.1574-695X.2006.00083.x (DOI)16831207 (PubMedID)
Note
The definitive version is available at www.blackwell-synergy.com:Johan Schöier, Marie Högdahl, Gustaf Söderlund and Erik Kihlström, Chlamydia (Chlamydophila) pneumoniae-induced cell death in human coronary artery endothelial cells is caspase-independent and accompanied by subcellular translocations of Bax and apoptosis-inducing factor., 2006, FEMS Immunology and Medical Microbiology, (47), 2, 207-216.http://dx.doi.org/10.1111/j.1574-695X.2006.00083.xCopyright: Blackwell Publishing Ltd. http://www.blackwellpublishing.com/and Scandinavian Societies for Medical Microbiology and PathologyAvailable from: 2009-03-07 Created: 2009-03-06 Last updated: 2017-12-13Bibliographically approved
Söderquist, B., Alriksson, I., Källman, J. & Kihlström, E. (2006). The influence of adhesive and invasive properties of Staphylococcus aureus defective in fibronectin-binding proteins on secretion of interleukin-6 by human endothelial cells. Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), 114(2), 112-116
Open this publication in new window or tab >>The influence of adhesive and invasive properties of Staphylococcus aureus defective in fibronectin-binding proteins on secretion of interleukin-6 by human endothelial cells
2006 (English)In: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), ISSN 0903-4641, E-ISSN 1600-0463, Vol. 114, no 2, p. 112-116Article in journal (Refereed) Published
Abstract [en]

Fibronectin-binding proteins (FnBP) are surface adhesins of Staphylococcus aureus documented to be virulence attributes in, for example, endovascular infections. By using mutants of S. aureus defective in the FnBPA and B genes we have investigated whether these adhesins affect cytokine expression in human umbilical vein endothelial cells (HUVEC). S. aureus expressing FnBPA and B adhered to and were internalized into HUVEC to a greater extent compared to mutants defective in expression of FnBP. Production and release of IL-6 was higher from endothelial cells infected with the parent FnBP-expressing strain compared to the FnBP-defective mutants. These results indicate that adhesion to and invasion of S. aureus into endothelial cells are important regulators of cytokine expression. © 2006 The Authors.

National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-35746 (URN)10.1111/j.1600-0463.2006.apm_319.x (DOI)28400 (Local ID)28400 (Archive number)28400 (OAI)
Available from: 2009-10-10 Created: 2009-10-10 Last updated: 2017-12-13
Coble, B.-I., Nordahl-Åkesson, E., Vinnerberg, Å. & Kihlström, E. (2006). Urine-based testing for Chlamydia trachomatis using polymerase chain reaction, leucocyte esterase and urethral and cervical smears. Scandinavian Journal of Clinical and Laboratory Investigation, 66(4), 269-278
Open this publication in new window or tab >>Urine-based testing for Chlamydia trachomatis using polymerase chain reaction, leucocyte esterase and urethral and cervical smears
2006 (English)In: Scandinavian Journal of Clinical and Laboratory Investigation, ISSN 0036-5513, E-ISSN 1502-7686, Vol. 66, no 4, p. 269-278Article in journal (Refereed) Published
Abstract [en]

The performance of Roche polymerase chain reaction (PCR) Amplicor to detect Chlamydia trachomatis in first-voided urine specimens from 422 males and 456 females attending two clinics for sexually transmitted infections was evaluated in comparison with cultures of urethral and cervical specimens. At the same time, the ability of leucocyte esterase (LE) in first-voided urine and the presence of leucocytes in urethral and cervical smears to identify C. trachomatis -infected individuals based on PCR and culture was determined. The prevalence of C. trachomatis infection was 10.9 % in men and 7.7 % in women. Sensitivity, specificity, positive predictive value and negative predictive value of Amplicor was 93.5 %, 99.7 %, 97.7 % and 99.2 % in males and 91.4 %, 99.5 %, 94.1 % and 99.3 % in females. All Chlamydia-infected men were identified by means of a combination of urethritis (≥4 leucocytes in the urethral smear) and/or a positive LE test in urine, although the specificity was only 42.2 %. In women, the combination of urethritis and/or cervicitis and/or a positive LE test identified 85.7 % of Chlamydia-infected patients with a specificity of 38.2 %. It is concluded that a combination of urethral and/or cervical smears and LE testing of urine can be used as a screening test to select patients, especially males, for specific C. trachomatis testing.

National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-35605 (URN)10.1080/00365510600608266 (DOI)27913 (Local ID)27913 (Archive number)27913 (OAI)
Available from: 2009-10-10 Created: 2009-10-10 Last updated: 2017-12-13
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