liu.seSearch for publications in DiVA
Change search
Link to record
Permanent link

Direct link
BETA
Vikström, Elena
Alternative names
Publications (10 of 17) Show all publications
Everett, J., Gabrilska, R., Rumbaugh, K. P. & Vikström, E. (2018). Assessing Pseudomonas aeruginosa Autoinducer Effects on Mammalian Epithelial Cells. In: Livia LeoniGiordano Rampioni (Ed.), Quorum Sensing: Methods and Protocols (pp. 213-225). Humana Press, 1673
Open this publication in new window or tab >>Assessing Pseudomonas aeruginosa Autoinducer Effects on Mammalian Epithelial Cells
2018 (English)In: Quorum Sensing: Methods and Protocols / [ed] Livia LeoniGiordano Rampioni, Humana Press, 2018, Vol. 1673, p. 213-225Chapter in book (Refereed)
Abstract [en]

The human mucosal environment in the gut is rich with interactions between microbiota and mammalian epithelia. Microbes such as the Gram-negative bacterium Pseudomonas aeruginosa may use quorum sensing to communicate with other microorganisms and mammalian cells to alter gene expression. Here, we present methodologies to evaluate the effects of P. aeruginosa N-(3-oxo-dodecanoyl)-L-homoserine lactone (3O-C12-HSL) on Caco-2 cell monolayers. First, we describe a method for assessing barrier function and permeability of epithelial cells when exposed to 3O-C12-HSL by measuring transepithelial electrical resistance (TER) and paracellular flow using fluorescently labeled dextran. Secondly, we detail methods to investigate the effect of 3O-C12-HSL on protein-protein interactions of epithelial junction proteins. Lastly, we will detail imaging techniques to visualize Caco-2 barrier disruption following exposure to 3O-C12-HSL through the use of confocal laser scanning microscopy (CLSM) and a super resolution technique, stimulated emission depletion (STED) microscopy, to achieve nanoscale visualization of Caco-2 monolayers.

Place, publisher, year, edition, pages
Humana Press, 2018
Series
Methods in Molecular Biology, ISSN 1064-3745, E-ISSN 1940-6029 ; 1673
Keywords
Acyl homoserine lactone; Cell junction; Host pathogen interaction; IQGAP1; Imaging; Paracellular permeability; Pseudomonas aeruginosa; Quorum sensing; Transepithelial electrical resistance
National Category
Cell Biology
Identifiers
urn:nbn:se:liu:diva-152539 (URN)10.1007/978-1-4939-7309-5_17 (DOI)29130176 (PubMedID)9781493973088 (ISBN)9781493973095 (ISBN)
Available from: 2019-03-27 Created: 2019-03-27 Last updated: 2019-03-27Bibliographically approved
Molinas, A., Mirazimi, A., Holm, A., Loitto, V. M., Magnusson, K.-E. & Vikström, E. (2016). Protective role of host aquaporin 6 against Hazara virus, a model for Crimean–Congo hemorrhagic fever virus infection. FEMS Microbiology Letters, 363(8), Article ID fnw058.
Open this publication in new window or tab >>Protective role of host aquaporin 6 against Hazara virus, a model for Crimean–Congo hemorrhagic fever virus infection
Show others...
2016 (English)In: FEMS Microbiology Letters, ISSN 0378-1097, E-ISSN 1574-6968, Vol. 363, no 8, article id fnw058Article in journal (Refereed) Published
Abstract [en]

Crimean–Congo hemorrhagic fever virus (CCHFV) is an arthropod-borne pathogen that causes infectious disease with severe hemorrhagic manifestations in vascular system in humans. The proper function of the cells in the vascular system is critically regulated by aquaporins (AQP), water channels that facilitate fluxes of water and small solutes across membranes. With Hazara virus as a model for CCHFV, we investigated the effects of viruses on AQP6 and the impact of AQP6 on virus infectivity in host cells, using transiently expressed GFP-AQP6 cells, immunofluorescent assay for virus detection, epifluorescent imaging of living cells and confocal microscopy. In GFP-AQP6 expressing cells, Hazara virus reduced both the cellular and perinuclear AQP6 distribution and changed the cell area. Infection of human cell with CCHFV strain IbAR 10200 downregulated AQP6 expression at mRNA level. Interestingly, the overexpression of AQP6 in host cells decreased the infectivity of Hazara virus, speaking for a protective role of AQP6. We suggest the possibility for AQP6 being a novel player in the virus–host interactions, which may lead to less severe outcomes of an infection.

Place, publisher, year, edition, pages
Oxford University Press, 2016
Keywords
Host–virus interactions; Nairovirus; Crimean–Congo hemorrhagic fever virus; aquaporin; virus infectivity; water homeostasis
National Category
Cell and Molecular Biology Microbiology in the medical area
Identifiers
urn:nbn:se:liu:diva-127499 (URN)10.1093/femsle/fnw058 (DOI)000377970600013 ()26976854 (PubMedID)
Funder
Swedish Research Council, 2010-3045European Science Foundation (ESF)Magnus Bergvall FoundationSwedish Research Council, 214–7495Linköpings universitet
Note

Funding agencies: Swedish Research Council [2010-3045]; European Science foundation; Magnus Bergvall Foundation; Faculty of Medicine and Health Sciences, Linkoping University; Infect-ERA Second Call (Swedish Research Council) [214-7495]

Available from: 2016-04-28 Created: 2016-04-28 Last updated: 2018-01-10Bibliographically approved
Holm, A., Magnusson, K.-E. & Vikström, E. (2016). Pseudomonas aeruginosa N-3-oxo-dodecanoyl-homoserine Lactone Elicits Changes in Cell Volume, Morphology, and AQP9 Characteristics in Macrophages. Frontiers in Cellular and Infection Microbiology, 6(32)
Open this publication in new window or tab >>Pseudomonas aeruginosa N-3-oxo-dodecanoyl-homoserine Lactone Elicits Changes in Cell Volume, Morphology, and AQP9 Characteristics in Macrophages
2016 (English)In: Frontiers in Cellular and Infection Microbiology, E-ISSN 2235-2988, ISSN 2235-2988, Vol. 6, no 32Article in journal (Refereed) Published
Abstract [en]

Quorum sensing (QS) communication allows Pseudomonas aeruginosa to collectively control its population density and the production of biofilms and virulence factors. QS signal molecules, like N-3-oxo-dodecanoyl-L-homoserine lactone (30-C-12-HSL), can also affect the behavior of host cells, e.g., by modulating the chemotaxis, migration, and phagocytosis of human leukocytes. Moreover, host water homeostasis and water channels aquaporins (AQP) are critical for cell morphology and functions as AQP interact indirectly with the cell cytoskeleton and signaling cascades. Here, we investigated how P aeruginosa 30-C-12-HSL affects cell morphology, area, volume and AQP9 expression and distribution in human primary macrophages, using quantitative PCR, immunoblotting, two- and three-dimensional live imaging, confocal and nanoscale imaging. Thus, 30-C-12-HSL enhanced cell volume and area and induced cell shape and protrusion fluctuations in macrophages, processes tentatively driven by fluxes of water across cell membrane through AQP9, the predominant AQP in macrophages. Moreover, 30-C-12-HSL upregulated the expression of AQP9 at both the protein and mRNA levels. This was accompanied with enhanced whole cell AQP9 fluorescent intensity and redistribution of AQP9 to the leading and trailing regions, in parallel with increased cell area in the macrophages. Finally, nanoscopy imaging provided details on AQP9 dynamics and architecture within the lamellipodial area of 30-C-12-HSL-stimulated cells. We suggest that these novel events in the interaction between P aeruginosa and macrophage may have an impact on the effectiveness of innate immune cells to fight bacteria, and thereby resolve the early stages of infections and inflammations.

Place, publisher, year, edition, pages
FRONTIERS MEDIA SA, 2016
Keywords
host-bacteria interactions; quorum sensing; N-acylhomoserinelactone; innate immunity; macrophage; water homeostasis; aquaporin
National Category
Clinical Medicine
Identifiers
urn:nbn:se:liu:diva-127262 (URN)10.3389/fcimb.2016.00032 (DOI)000372710500001 ()27047801 (PubMedID)
Note

Funding Agencies|Swedish Research Council [2010-3045]; European Science foundation (TraPPs Euromembrane project); Magnus Bergvall Foundation; Faculty of Medicine and Health Sciences, Linkoping University

Available from: 2016-04-20 Created: 2016-04-19 Last updated: 2018-05-14
Turkina, M., Olofsson, A., Magnusson, K.-E., Arnqvist, A. & Vikström, E. (2015). Helicobacter pylori vesicles carrying CagA localize in the vicinity of cell-cell contacts and induce histone H1 binding to ATP in epithelial cells. FEMS Microbiology Letters, 362(11), fnv076
Open this publication in new window or tab >>Helicobacter pylori vesicles carrying CagA localize in the vicinity of cell-cell contacts and induce histone H1 binding to ATP in epithelial cells
Show others...
2015 (English)In: FEMS Microbiology Letters, ISSN 0378-1097, E-ISSN 1574-6968, Vol. 362, no 11, p. fnv076-Article in journal (Refereed) Published
Abstract [en]

Helicobacter pylori produces outer membrane vesicles (OMV), delivering bacterial substances including the oncogenic cytotoxin-associated CagA protein to their surroundings. We investigated the effects of H. pylori OMV carrying CagA (OMV-CagA) on cell junctions and ATP-binding proteome of epithelial monolayers, using proteomics, mass spectrometry and imaging. OMV-CagA localized in close vicinity of ZO-1 tight junction protein and induced histone H1 binding to ATP. We suggest the expression of novel events in the interactions between H. pylori OMV and epithelia, which may have an influence on host gene transcription and lead to different outcomes of an infection and development of cancer.

Place, publisher, year, edition, pages
Oxford University Press (OUP): Policy B - Oxford Open Option D, 2015
Keywords
Helicobacter pylori; outer membrane vesicles; CagA; ATP-proteome; histone H1; epithelial cell-cell junctions
National Category
Clinical Medicine
Identifiers
urn:nbn:se:liu:diva-120236 (URN)10.1093/femsle/fnv076 (DOI)000356890900007 ()25956174 (PubMedID)
Note

Funding Agencies|Swedish Research Council [2005-4636, 2008-3000, 2014-4361, 2007-3483, 2010-3045]; Euro-BioImaging; Magnus Bergvalls Foundation; Faculty of Health Sciences, Linkoping University; Kempe Memorial Foundation; Cancerfonden [CAN 2012/759]; Cancerforskningsfonden i Norrland; Insamlingstiftelsen for medicinsk forskning vid Umea universitet

Available from: 2015-07-21 Created: 2015-07-20 Last updated: 2017-12-04
Holm, A., Karlsson, T. & Vikström, E. (2015). Pseudomonas aeruginosa lasI/rhlI quorum sensing genes promote phagocytosis and aquaporin 9 redistribution to the leading and trailing regions in macrophages. Frontiers in Microbiology, 6(915)
Open this publication in new window or tab >>Pseudomonas aeruginosa lasI/rhlI quorum sensing genes promote phagocytosis and aquaporin 9 redistribution to the leading and trailing regions in macrophages
2015 (English)In: Frontiers in Microbiology, ISSN 1664-302X, E-ISSN 1664-302X, Vol. 6, no 915Article in journal (Refereed) Published
Abstract [en]

Pseudomonas aeruginosa controls production of its multiple virulence factors and biofilm development via the quorum sensing (QS) system. QS signals also interact with and affect the behavior of eukaryotic cells. Host water homeostasis and aquaporins (AQP) are essential during pathological conditions since they interfere with the cell cytoskeleton and signaling, and hereby affect cell morphology and functions. We investigated the contribution of F? aeruginosa QS genes lasl/rhIl to phagocytosis, cell morphology, AQP9 expression, and distribution in human macrophages, using immunoblotting, confocal, and nanoscale imaging. Wild type F? aeruginosa with a functional QS system was a more attractive prey for macrophages than the lasl/rhIl mutant lacking the production of QS molecules, 30-C-12-HSL, and C-4 -HSL, and associated virulence factors. The F? aeruginosa infections resulted in elevated AQP9 expression and relocalization to the leading and trailing regions in macrophages, increased cell area and length; bacteria with a functional QS system lasl/rhIl achieved stronger responses. We present evidence for a new role of water fluxes via AQP9 during bacteria macrophage interaction and for the QS system as an important stimulus in this process. These novel events in the interplay between F? aeruginosa and macrophages may influence on the outcome of infection, inflammation, and development of disease.

Place, publisher, year, edition, pages
FRONTIERS MEDIA SA, 2015
Keywords
host-bacteria relationship; quorum sensing; N-acylhomoserine lactone; innate immunity; macrophage; water homeostasis; aquaporin
National Category
Clinical Medicine
Identifiers
urn:nbn:se:liu:diva-122056 (URN)10.3389/fmicb.2015.00915 (DOI)000360626200001 ()26388857 (PubMedID)
Note

Funding Agencies|Swedish Research Council [2010-3045]; European Science foundation (TraPPs Euromembrane project); Euro-BioImaging Proof-of Concept Studies; Magnus Bergvalls Foundation; Faculty of Health Sciences, Linkoping University

Available from: 2015-12-18 Created: 2015-10-19 Last updated: 2017-12-01
Holm, A. & Vikström, E. (2014). Quorum sensing communication between bacteria and human cells: signals, targets, and functions. Frontiers in Plant Science, 5(309)
Open this publication in new window or tab >>Quorum sensing communication between bacteria and human cells: signals, targets, and functions
2014 (English)In: Frontiers in Plant Science, ISSN 1664-462X, E-ISSN 1664-462X, Vol. 5, no 309Article, review/survey (Refereed) Published
Abstract [en]

Both direct and long-range interactions between pathogenic Pseudomonas aeruginosa bacteria and their eukaryotic hosts are important in the outcome of infections. For cell-to-cell communication, these bacteria employ the quorum sensing (QS) system to pass on information of the density of the bacterial population and collectively switch on virulence factor production, biofilm formation, and resistance development. Thus, QS allows bacteria to behave as a community to perform tasks which would be impossible for individual cells, e.g., to overcome defense and immune systems and establish infections in higher organisms. This review highlights these aspects of QS and our own recent research on how P aeruginosa communicates with human cells using the small QS signal molecules N-acyl homoserine lactones (AHL). We focus on how this conversation changes the behavior and function of neutrophils, macrophages, and epithelial cells and on how the signaling machinery in human cells responsible for the recognition of AHL. Understanding the bacteria host relationships at both cellular and molecular levels is essential for the identification of new targets and for the development of novel strategies to fight bacterial infections in the future.

Place, publisher, year, edition, pages
Frontiers, 2014
Keywords
bacteria-host cell interaction; quorum sensing; Pseudomonas aeruginosa; N-acyl homoserine lactones; epithelial cells; innate immune cells; neutrophils; macrophages
National Category
Clinical Medicine
Identifiers
urn:nbn:se:liu:diva-109601 (URN)10.3389/fpls.2014.00309 (DOI)000339441800001 ()25018766 (PubMedID)
Available from: 2014-08-21 Created: 2014-08-21 Last updated: 2017-12-05
Istrate, C., Hagbom, M., Vikström, E., Magnusson, K.-E. & Svensson, L. (2014). Rotavirus Infection Increases Intestinal Motility but Not Permeability at the Onset of Diarrhea. Journal of Virology, 88(6), 3161-3169
Open this publication in new window or tab >>Rotavirus Infection Increases Intestinal Motility but Not Permeability at the Onset of Diarrhea
Show others...
2014 (English)In: Journal of Virology, ISSN 0022-538X, E-ISSN 1098-5514, Vol. 88, no 6, p. 3161-3169Article in journal (Refereed) Published
Abstract [en]

The disease mechanisms associated with onset and secondary effects of rotavirus (RV) diarrhea remain to be determined and may not be identical. In this study, we investigated whether onset of RV diarrhea is associated with increased intestinal permeability and/or motility. To study the transit time, fluorescent fluorescein isothiocyanate (FITC)-dextran was given to RV-infected adult and infant mice. Intestinal motility was also studied with an opioid receptor agonist (loperamide) and a muscarinic receptor antagonist (atropine). To investigate whether RV increases permeability at the onset of diarrhea, fluorescent 4- and 10-kDa dextran doses were given to infected and noninfected mice, and fluorescence intensity was measured subsequently in serum. RV increased transit time in infant mice. Increased motility was detected at 24 h postinfection (h p.i.) and persisted up to 72 h p.i in pups. Both loperamide and atropine decreased intestinal motility and attenuated diarrhea. Analysis of passage of fluorescent dextran from the intestine into serum indicated unaffected intestinal permeability at the onset of diarrhea (24 to 48 h p.i.). We show that RV-induced diarrhea is associated with increased intestinal motility via an activation of the myenteric nerve plexus, which in turn stimulates muscarinic receptors on intestinal smooth muscles.

Place, publisher, year, edition, pages
American Society for Microbiology, 2014
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-105750 (URN)10.1128/JVI.02927-13 (DOI)000332126000010 ()
Available from: 2014-04-07 Created: 2014-04-04 Last updated: 2017-12-05
Karlsson, T., Lagerholm, C. B., Vikström, E., Loitto, V. & Magnusson, K.-E. (2013). Water fluxes through aquaporin-9 prime epithelial cells for rapid wound healing. Biochemical and Biophysical Research Communications - BBRC, 430(3), 993-998
Open this publication in new window or tab >>Water fluxes through aquaporin-9 prime epithelial cells for rapid wound healing
Show others...
2013 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 430, no 3, p. 993-998Article in journal (Refereed) Published
Abstract [en]

Cells move along surfaces both as single cells and multi-cellular units. Recent research points toward pivotal roles for water flux through aquaporins (AQPs) in single cell migration. Their expression is known to facilitate this process by promoting rapid shape changes. However, little is known about the impact on migrating epithelial sheets during wound healing and epithelial renewal. Here, we investigate and compare the effects of AQP9 on single cell and epithelial sheet migration. To achieve this, MDCK-1 cells stably expressing AQP9 were subjected to migration assessment. We found that AQP9 facilitated cell locomotion at both the single and multi-cellular level. Furthermore, we identified major differences in the monolayer integrity and cell size upon expression of AQP9 during epithelial sheet migration, indicating a rapid volume-regulatory mechanism. We suggest a novel mechanism for epithelial wound healing based on AQP-induced swelling and expansion of the monolayer.

Place, publisher, year, edition, pages
Elsevier, 2013
Keywords
Cell migration, Aquaporins, AQP9, Wound healing, Cell motility
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-89749 (URN)10.1016/j.bbrc.2012.11.125 (DOI)000314376100021 ()
Note

Funding Agencies|Swedish Research Council for Medicine and Health|2007-34832009-66492010-3045|

Available from: 2013-03-05 Created: 2013-03-05 Last updated: 2017-12-06
Karlsson, T., Musse, F., Magnusson, K.-E. & Vikström, E. (2012). N-Acylhomoserine lactones are potent neutrophil chemoattractants that act via calcium mobilization and actin remodeling. Journal of Leukocyte Biology, 91(1), 15-26
Open this publication in new window or tab >>N-Acylhomoserine lactones are potent neutrophil chemoattractants that act via calcium mobilization and actin remodeling
2012 (English)In: Journal of Leukocyte Biology, ISSN 0741-5400, E-ISSN 1938-3673, Vol. 91, no 1, p. 15-26Article in journal (Refereed) Published
Abstract [en]

In gram-negative bacteria, cell-cell communication based on HSL QS molecules is known to coordinate the production of virulence factors and biofilms. These bacterial signals can also modulate human immune cell behavior. Using a Transwell migration assay, we found that human primary neutrophils are strongly stimulated by 3O-C(12)-HSL and -C(10)-HSL but not C(4)-HSL in a concentration-dependent manner. Moreover, 3O-C(12)-HSL and -C(10)-HSL activate PLC gamma 1 but not -gamma 2, mobilize intracellular calcium, and up-regulate IP(3)R. These changes were paralleled by F-actin accumulation, primarily in the leading edge of neutrophils, as evidenced by phalloidin staining and confocal microscopy. F- and G-actin isolation and quantification by immunoblotting revealed that the F/G-actin ratio was increased significantly after treatment with all three HSLs. Furthemore, 3O-C(12)-HSL- and 3O-C(10)-HSL treatment resulted in phosphorylation of Rac1 and Cdc42. In contrast, C(4)-HSL had negligible influence on the phosphorylation status of PLC and Rac1/Cdc42 and failed to attract neutrophils and induce calcium release. The calcium inhibitor thapsigargin, which blocks ER calcium uptake, strongly prevented neutrophil migration toward 3O-C(12)-HSL and -C(10)-HSL. These findings show that the bacterial QS molecules 3O-C(12)-HSL and -C(10)-HSL may attract human neutrophils to the sites of bacterial infection and developing biofilms. Indeed, recognition of HSL QS signals by neutrophils may play a critical role in their recruitment during infections.

Place, publisher, year, edition, pages
Society for Leukocyte Biology, 2012
Keywords
quorum sensing, migration, Ca(2+), PLC, Rho-GTPases, cytoskeleton
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-74855 (URN)10.1189/jlb.0111034 (DOI)000299168600004 ()
Note
Funding Agencies|Swedish Research Council||European Science Foundation||King Gustaf V 80-Year Foundation||Faculty of Health Sciences, Linkoping University (Sweden)||Available from: 2012-02-10 Created: 2012-02-10 Last updated: 2017-12-07
Karlsson, T., Turkina, M., Yakymenko, O., Magnusson, K.-E. & Vikström, E. (2012). The Pseudomonas aeruginosa N-Acylhomoserine Lactone Quorum Sensing Molecules Target IQGAP1 and Modulate Epithelial Cell Migration. PLOS PATHOGENS, 8(10)
Open this publication in new window or tab >>The Pseudomonas aeruginosa N-Acylhomoserine Lactone Quorum Sensing Molecules Target IQGAP1 and Modulate Epithelial Cell Migration
Show others...
2012 (English)In: PLOS PATHOGENS, ISSN 1553-7374, Vol. 8, no 10Article in journal (Refereed) Published
Abstract [en]

Quorum sensing (QS) signaling allows bacteria to control gene expression once a critical population density is achieved. The Gram-negative human pathogen Pseudomonas aeruginosa uses N-acylhomoserine lactones (AHL) as QS signals, which coordinate the production of virulence factors and biofilms. These bacterial signals can also modulate human cell behavior. Little is known about the mechanisms of the action of AHL on their eukaryotic targets. Here, we found that N-3-oxododecanoyl- L-homoserine lactone 3O-C-12-HSL modulates human intestinal epithelial Caco-2 cell migration in a dose- and time-dependent manner. Using new 3O-C-12-HSL biotin and fluorescently-tagged probes for LC-MS/MS and confocal imaging, respectively, we demonstrated for the first time that 3O-C-12-HSL interacts and co-localizes with the IQ-motif-containing GTPase-activating protein IQGAP1 in Caco-2 cells. The interaction between IQGAP1 and 3O-C-12-HSL was further confirmed by pull-down assay using a GST-tagged protein with subsequent Western blot of IQGAP1 and by identifying 3O-C-12-HSL with a sensor bioassay. Moreover, 3O-C-12-HSL induced changes in the phosphorylation status of Rac1 and Cdc42 and the localization of IQGAP1 as evidenced by confocal and STED microscopy and Western blots. Our findings suggest that the IQGAP1 is a novel partner for P. aeruginosa 3O-C-12-HSL and likely the integrator of Rac1 and Cdc42- dependent altered cell migration. We propose that the targeting of IQGAP1 by 3O-C-12-HSL can trigger essential changes in the cytoskeleton network and be an essential component in bacterial - human cell communication.

Place, publisher, year, edition, pages
Public Library of Science, 2012
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-86387 (URN)10.1371/journal.ppat.1002953 (DOI)000310530300018 ()
Note

Funding Agencies|Swedish Research Council||European Science foundation||TraPPs Euromembrane project||King Gustaf V 80-Year Foundation||Faculty of Health Sciences, Linkoping University||

Available from: 2012-12-14 Created: 2012-12-14 Last updated: 2013-01-14
Organisations

Search in DiVA

Show all publications