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Björk, Per
Publications (10 of 16) Show all publications
Björk, P., Herland, A., Hamedi, M. & Inganäs, O. (2010). Biomolecular nanowires decorated by organic electronic polymers. JOURNAL OF MATERIALS CHEMISTRY, 20(12), 2269-2276
Open this publication in new window or tab >>Biomolecular nanowires decorated by organic electronic polymers
2010 (English)In: JOURNAL OF MATERIALS CHEMISTRY, ISSN 0959-9428, Vol. 20, no 12, p. 2269-2276Article in journal (Refereed) Published
Abstract [en]

We demonstrate the shaping and forming of organic electronic polymers into designer nanostructures using biomacromolecules. In order to create nanowires, nanohelixes and assemblies of these, we coordinate semiconducting or metallic polymers to biomolecular polymers in the form of DNA and misfolded proteins. Optoelectronic and electrochemical devices utilizing these shaped materials are discussed.

National Category
Engineering and Technology
Identifiers
urn:nbn:se:liu:diva-54609 (URN)10.1039/b910639a (DOI)000275380000001 ()
Available from: 2010-03-26 Created: 2010-03-26 Last updated: 2010-03-26
Wigenius, J., Magnusson, K., Björk, P., Andersson, O. & Inganäs, O. (2010). DNA Chips with Conjugated Polyelectrolytes in Resonance Energy Transfer Mode. LANGMUIR, 26(5), 3753-3759
Open this publication in new window or tab >>DNA Chips with Conjugated Polyelectrolytes in Resonance Energy Transfer Mode
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2010 (English)In: LANGMUIR, ISSN 0743-7463, Vol. 26, no 5, p. 3753-3759Article in journal (Refereed) Published
Abstract [en]

We show how to use well-defined conjugated polyelectrolytes (CPEs) combined With Surface energy patterning to Fabricate DNA Chips utilizing A fluorescence signal amplification. Cholesterol-modified DNA strands in complex with it CPE are adsorbed to a surface energy pattern, formed by printing with soft elastomer stamps. Hybridization of the surface bound DNA strands with it short complementary strand from Solution is monitored using both fluorescence microscopy and imaging surface plasmon resonance. The CPEs act as antennas, enhancing resonance energy transfer to the dye-labeled DNA when complementary hybridization of the double strand occurs.

National Category
Engineering and Technology
Identifiers
urn:nbn:se:liu:diva-54255 (URN)10.1021/la903101v (DOI)000274636900113 ()
Available from: 2010-03-05 Created: 2010-03-05 Last updated: 2015-05-29
Hamedi, M., Wigenius, J., Tai, F.-i., Björk, P. & Aili, D. (2010). Polypeptide-guided assembly of conducting polymer nanocomposites. NANOSCALE, 2(10), 2058-2061
Open this publication in new window or tab >>Polypeptide-guided assembly of conducting polymer nanocomposites
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2010 (English)In: NANOSCALE, ISSN 2040-3364, Vol. 2, no 10, p. 2058-2061Article in journal (Refereed) Published
Abstract [en]

A strategy for fabrication of electroactive nanocomposites with nanoscale organization, based on self-assembly, is reported. Gold nanoparticles are assembled by a polypeptide folding-dependent bridging. The polypeptides are further utilized to recruit and associate with a water soluble conducting polymer. The polymer is homogenously incorporated into the nanocomposite, forming conducting pathways which make the composite material highly conducting.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2010
National Category
Engineering and Technology
Identifiers
urn:nbn:se:liu:diva-61181 (URN)10.1039/c0nr00299b (DOI)000282686200019 ()
Note
Original Publication: Mahiar Hamedi, Jens Wigenius, Feng-i Tai, Per Björk and Daniel Aili, Polypeptide-guided assembly of conducting polymer nanocomposites, 2010, NANOSCALE, (2), 10, 2058-2061. http://dx.doi.org/10.1039/c0nr00299b Copyright: Royal Society of Chemistry http://www.rsc.org/ Available from: 2010-11-05 Created: 2010-11-05 Last updated: 2014-10-08Bibliographically approved
Wigenius, J., Björk, P., Hamedi, M. & Aili, D. (2010). Supramolecular Assembly of Designed α-Helical Polypeptide-Based Nanostructures and Luminescent Conjugated Polyelectrolytes. Macromolecular Bioscience, 10(8), 836-841
Open this publication in new window or tab >>Supramolecular Assembly of Designed α-Helical Polypeptide-Based Nanostructures and Luminescent Conjugated Polyelectrolytes
2010 (English)In: Macromolecular Bioscience, ISSN 1616-5187, E-ISSN 1616-5195, Vol. 10, no 8, p. 836-841Article in journal (Refereed) Published
Abstract [en]

Designed polypeptides with controllable folding properties are utilized as supramolecular templates for fabrication of ordered nanoscale molecular and fibrous assemblies of luminescent conjugated polymers (LCPs). The properties of the LCPs as well as the three dimensional conformation of the polypeptide-scaffold determine how the polymers are arranged in the supramolecular construct, which highly affects the properties of the hybrid material. The ability to control the polypeptide conformation and assembly into fibers provide a promising route for tuning the optical properties of LCPs and for fabrication of complex functional supramolecules with well defined structural properties.

Place, publisher, year, edition, pages
John Wiley & Sons, 2010
Keywords
α-helical; conjugated polymers; hybrid materials; peptides; self-assembly
National Category
Natural Sciences
Identifiers
urn:nbn:se:liu:diva-54899 (URN)10.1002/mabi.200900463 (DOI)000281387900002 ()
Available from: 2010-04-20 Created: 2010-04-20 Last updated: 2017-12-12Bibliographically approved
Hamedi, M., Wigenius, J., Tai, F.-I., Björk, P. & Aili, D. (2010). Synthetic Polypeptides as Scaffolds for Supramolecular Assembly of Conducting Polymer Nanocomposites.
Open this publication in new window or tab >>Synthetic Polypeptides as Scaffolds for Supramolecular Assembly of Conducting Polymer Nanocomposites
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2010 (English)Manuscript (preprint) (Other academic)
Abstract [en]

The development of nanoelectronics has resulted in enormous advancements in fabrication techniques that have enabled massproduction of CMOS circuits with feature sizes below 45nm. There is a large interest in new methods to further push the size limits, lower the production costs and to facilitate the design of more advanced three-dimensional structures beyond today’s 2.5 dimensional architectures. Self-assembly is probably the most important scheme in this development and is currently applied to many different areas and classes of nanoelectronics. Self-assembly enables fabrication of structures well below 10 nm in feature size and allows for incorporation of novel nanomaterials, such as metallic and semiconducting nanoparticles with many interesting optical and electrical properties. The controlled self-assembly of electro-active nanocomposites is of great interest for the development of novel functional materials including biosensors, electrochromic/plasmonic hybrid devices, and polymer/nanoparticle-based memories.

Keywords
Conducting Polymers, Organic Electronics, Conducting Nanowires, Self- Assembly, Supramolecular Materials
National Category
Natural Sciences
Identifiers
urn:nbn:se:liu:diva-54901 (URN)
Available from: 2010-04-20 Created: 2010-04-20 Last updated: 2010-04-20
Björk, P., Thomsson, D., Mirzov, O., Andersson, J., Inganäs, O. & Scheblykin , I. G. (2009). Oligothiophene Assemblies Defined by DNA Interaction: From Single Chains to Disordered Clusters. SMALL, 5(1), 96-103
Open this publication in new window or tab >>Oligothiophene Assemblies Defined by DNA Interaction: From Single Chains to Disordered Clusters
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2009 (English)In: SMALL, ISSN 1613-6810 , Vol. 5, no 1, p. 96-103Article in journal (Refereed) Published
Abstract [en]

The organization of conjugated polyelectrolytes (CPEs) interacting with biomolecules sets conditions for the biodetection of biological processes and identity, through the use of optical emission from the CPE. Herein, a well-defined CPE and its binding to DNA is studied. By using dynamic light scattering and circular dichroism spectroscopy, it is shown that the CPE forms a multimolecule ensemble in aqueous solution that is more than doubled it? size when interacting with a small DNA chain, while single chains are evident in ethanol. The related changes in the fluorescence spectra upon polymer aggregation are assigned to oscillator strength redistribution between vibronic transitions in weakly coupled H-aggregates. An enhanced single-molecule spectroscopy technique that allows full control of excitation and emission light polarization is applied to combed and decorated;,DNA chains. It is found that the organization of combed CPE-lambda DNA complexes (when dry on the surface) allows considerable variation of CPE distances and direction relative to the DNA chain. By analysis of the polarization data. energy transfer between the polymer chains in individual complexes is confirmed and their sizes estimated.

Keywords
aggregation, conjugated polymers, DNA, fluorescence, single-molecule spectroscopy
National Category
Natural Sciences
Identifiers
urn:nbn:se:liu:diva-16829 (URN)10.1002/smll.200800855 (DOI)
Available from: 2009-02-20 Created: 2009-02-20 Last updated: 2010-04-20
Herland, A., Björk, P., Hania, P. R., Scheblykin, I. G. & Inganäs, O. (2007). Alignment of a conjugated polymer onto amyloid-like protein fibrils. Small, 3(2), 318-325
Open this publication in new window or tab >>Alignment of a conjugated polymer onto amyloid-like protein fibrils
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2007 (English)In: Small, ISSN 1613-6810, Vol. 3, no 2, p. 318-325Article in journal (Refereed) Published
Abstract [en]

The amyloid-like fibril is a biomolecular nanowire template of very high stability. Here we describe the coordination of a conjugated polyelectrolyte, poly(thiophene acetic acid) (PTAA), to bovine insulin fibrils with widths of <10 nm and lengths of up to more than 10 m. Fibrils complexed with PTAA are aligned on surfaces through molecular combing and transfer printing. Single-molecule spectroscopy techniques are applied to chart spectral variation in the emission of these wires. When these results are combined with analysis of the polarization of the emitted light, we can conclude that the polymer chains are preferentially aligned along the fibrillar axis.

Keywords
fibrils, nanowires, polymers, self-assembly, template synthesis
National Category
Physical Sciences
Identifiers
urn:nbn:se:liu:diva-14602 (URN)10.1002/smll.200600377 (DOI)
Available from: 2007-10-12 Created: 2007-10-12 Last updated: 2009-04-29
Björk, P. (2007). Biological Sensing and DNA Templated Electronics Using Conjugated Polymers. (Doctoral dissertation). Institutionen för fysik, kemi och biologi
Open this publication in new window or tab >>Biological Sensing and DNA Templated Electronics Using Conjugated Polymers
2007 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Conjugated polymers have been found useful in a wide range of applications such as solar cells, sensor elements and printed electronics, due to their optical and electronic properties. Functionalization with charged side chains has enabled water solubility, resulting in an enhanced interaction with biomolecules. This thesis focus on the emerging research fields, where these conjugated polyelectrolytes (CPEs) are combined with biomolecules for biological sensing and DNA nanowire assembling.

CPEs have shown large potential in biomolecular detection where the optical read out is due to the geometrical alternation in the backbone and aggregation state. This thesis focused on transferring the biomolecular detection to a surface of CPEs. The characterization of the CPE layer show that a hydrogel can be formed, and how the layer can undergo geometrical changes upon external stimulus such as pH change. A selective sensor surface can be created by imprinting ssDNA or an antibody in the CPE layer. The discrimination for complementary DNA hybridization and specific antibody interaction can be monitored by surface plasmon resonance or quartz crystal microbalance. We have also taken the step out from the controlled test tube experiments to the complex environment of the cell showing the potential for staining of compartments and structures in live and fixed cell. Depending on the conditions and CPE used, cell nuclei, acidic vesicles and cytoskeleton structure can be visualized. Furthermore, the live staining shows no sign of toxic effect on cultured fibroblasts.

CPEs can also be a valuable element when assembling electronics in the true nano regime. I have used DNA as building template due to its attractive size features, with a width of around 2 nm and a length scale in the µm regime, and the inbuilt base-paring recognition elements. This thesis shows how DNA can be decorated with CPEs and stretched on surfaces into a model for aligned semiconducting nanowire geometries. Not only making the template structures is of importance, but also how to place them on the correct surface position, i.e. on electrodes. Strategies for positioning DNA nanowires using transfer printing and surface energy patterning methods have therefore been developed in the thesis. The stretched DNA decorated with CPE also offers a way to further study the molecular binding interaction between the two molecules. Single molecular spectroscopy in combination with polarization has given information of the variation of the CPE binding along a DNA chain.

Place, publisher, year, edition, pages
Institutionen för fysik, kemi och biologi, 2007
Series
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 1154
Keywords
conjugated polymer, conjugated polyelectrolyte, DNA, sensor, nanowire
National Category
Industrial Biotechnology
Identifiers
urn:nbn:se:liu:diva-10180 (URN)978-91-85895-17-5 (ISBN)
Public defence
2007-12-14, Planck, Fysikhuset, Campus Valla, Linköpings Universitet, Linköping, 10:15 (English)
Opponent
Supervisors
Available from: 2007-12-07 Created: 2007-12-07 Last updated: 2020-03-24
Björk, P., Nilsson, P., Lenner, L., Kågedal, B., Persson, B., Inganäs, O. & Jonasson, J. (2007). Conjugated polythiophene probes target lysosome-related acidic vacuoles in cultured primary cells. Molecular and Cellular Probes, 21(5-6), 329-337
Open this publication in new window or tab >>Conjugated polythiophene probes target lysosome-related acidic vacuoles in cultured primary cells
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2007 (English)In: Molecular and Cellular Probes, ISSN 0890-8508, Vol. 21, no 5-6, p. 329-337Article in journal (Refereed) Published
Abstract [en]

Conformation-sensitive optical probes for studying biological processes and structures are of great interest. The present work shows for the first time that conjugated polyelectrolyte (CPE) probes can be used for specific targeting of chromatin, nuclear and cytoplasmatic vesicles, and cytoskeletal components in a complex system of cultured cells. One of the probes could also be used for vital staining of live cells. When bound to different entities, the polythiophene derivative probes emitted light with different colors due to the unique spectral properties of these conformation sensitive probes. The physical pre-requisites for binding could also be exploited for characterization of the target. Unexpectedly, lysosome-related acidic vacuoles were targeted in cultured primary cells by both anionic, cationic, and zwitter-ionic polythiophene derivatives. Pre-treatment with Bafilomycin A1, a specific inhibitor of vacuolar-type H+-ATPase, caused redistribution of the staining. The targeting of lysosome-related acidic vesicles could not be demonstrated in transformed cells (melanoma, neuroblastoma, and prostate cancer cell lines), indicating a difference in the localization, structure, accessibility, or quantity of the target in cultured normal cells as compared with the malignant cell lines. The chemical nature of the conjugated polyelectrolyte complex in the cytoplasmatic vacuoles remains elusive.

Keywords
Conjugated polyelectrolyte; Polythiophene; Fibroblast; Lysosome; Cancer
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-12727 (URN)10.1016/j.mcp.2007.04.005 (DOI)
Available from: 2007-12-07 Created: 2007-12-07 Last updated: 2014-04-08
Andersson, J., Björk, P., Herland, A., Tvingstedt, K. & Inganäs, O. (2006). Biomolecules and conjugated polyelectrolytes in patterning. In: NaPa spring meeting 06 Köpenhamn,2006.
Open this publication in new window or tab >>Biomolecules and conjugated polyelectrolytes in patterning
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2006 (English)In: NaPa spring meeting 06 Köpenhamn,2006, 2006Conference paper, Published paper (Other academic)
National Category
Natural Sciences
Identifiers
urn:nbn:se:liu:diva-37675 (URN)37290 (Local ID)37290 (Archive number)37290 (OAI)
Available from: 2009-10-10 Created: 2009-10-10
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