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Turkina, Maria V
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Publications (10 of 21) Show all publications
Ramezani, A., Eng, L., Turkina, M., Theodorsson, A. & Nayeri, F. (2018). A Sputum Screening Test to Rule Out Pneumonia at an Early Stage With High Negative Predictive Value. Point of Care, 17(4), 101-108
Open this publication in new window or tab >>A Sputum Screening Test to Rule Out Pneumonia at an Early Stage With High Negative Predictive Value
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2018 (English)In: Point of Care, ISSN 1533-029X, Vol. 17, no 4, p. 101-108Article in journal (Refereed) Published
Abstract [en]

Background Pneumonia is a serious and widespread cause of morbidity and mortality. At an early stage, the symptoms are similar to other respiratory disorders, and there is no single criterion standard for diagnosis. Antibiotics are used too often as a precaution.

Objectives The objective of this study was to perform an assessment and clinical evaluation of a rapid sputum screening test (index test) to rule out pneumonia.

Methods Leftover sputum samples (467) collected at the Department of Microbiology from November 2016 to March 2017 were blindly analyzed within 72 hours with the index test. The clinical accuracy of the test was estimated for pneumonia by comparison with the established diagnosis by independent physicians (International Classification of Diseases, 10th Revision). Hepatocyte growth factor and calprotectin were measured on random samples (80), and layman volunteers (40) were asked to perform the test on artificial samples.

Results Two of 73 cases of pneumonia (community-acquired and nosocomial) showed negative results by the sputum strip test (97% sensitivity and 94% negative predictive value). The test results were highly correlated to hepatocyte growth factor and calprotectin concentrations in samples (R 2 = 67% respective 39%). Importantly, all of the volunteers were able to estimate the correct positive and negative results.

Conclusions The novel rapid sputum test represents a feasible tool for screening and ruling out the overwhelming majority of nonsevere respiratory infections at primary care settings, at home or when properly equipped laboratories are not available.

Place, publisher, year, edition, pages
Wolters Kluwer, 2018
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-154614 (URN)10.1097/poc.0000000000000170 (DOI)
Available from: 2019-02-21 Created: 2019-02-21 Last updated: 2019-05-03
Hadrévi, J., Turkina, M., Carlsson, A., Gerdle, B., Larsson, B., Hellström, F. & Ghafouri, B. (2016). Myosin light chain and calcium regulating protein differences in chronic musculoskeletal neck and shoulder pain. Journal of Integrated OMICS, 6(1), 1-8
Open this publication in new window or tab >>Myosin light chain and calcium regulating protein differences in chronic musculoskeletal neck and shoulder pain
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2016 (English)In: Journal of Integrated OMICS, ISSN 2182-0287, Vol. 6, no 1, p. 1-8Article in journal (Refereed) Published
Abstract [en]

Proteomic screening analysis has detected myosin light chain (MLC) as a protein implied to be involved in chronic musculoskeletal neck and shoulder pain. Several analyses of MLC proteins have stated a difference in phosphorylation being the determining factor for protein activation hence altered contrability of the muscle in i.e. senescence. In continuation of a previous publication, this study is an attempt to analyze the different MLC isoforms by mass spectrometry and immune-analyses in myalgic and healthy trapezius muscle. In the present study no differences in phosphorylation level between the corresponding individual proteins were detected using LC-MSMS and immunoblotting; instead we assigned different isoforms of regulatory MLCs. To further elucidate the contrability: calcium (Ca2+) regulatory proteins, sarco(endo)plasmic reticulum Ca2+ ATPase 1 (SERCA-1) and calsequestrine (CSQ) were analyzed by western blot. The analysis revealed a significantly increased abundance of SERCA-1 protein in the myalgic muscle and a significantly increased abundance of CSQ in healthy muscle. Myalgic muscle contraction patterns have in previous studies shown to differ from healthy muscle which may be connected to the Ca2+ availability in the muscle. Here we present the proteomic characterization of differences in Ca2+ regulating proteins and particularly regulatory MLCs in trapezius muscle of women with chronic musculoskeletal neck and shoulder pain.

Place, publisher, year, edition, pages
Proteomass Scientific Society, 2016
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:liu:diva-130539 (URN)10.5584/jiomics.v6i1.191 (DOI)
Available from: 2016-08-15 Created: 2016-08-15 Last updated: 2018-01-17
Turkina, M., Olofsson, A., Magnusson, K.-E., Arnqvist, A. & Vikström, E. (2015). Helicobacter pylori vesicles carrying CagA localize in the vicinity of cell-cell contacts and induce histone H1 binding to ATP in epithelial cells. FEMS Microbiology Letters, 362(11), fnv076
Open this publication in new window or tab >>Helicobacter pylori vesicles carrying CagA localize in the vicinity of cell-cell contacts and induce histone H1 binding to ATP in epithelial cells
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2015 (English)In: FEMS Microbiology Letters, ISSN 0378-1097, E-ISSN 1574-6968, Vol. 362, no 11, p. fnv076-Article in journal (Refereed) Published
Abstract [en]

Helicobacter pylori produces outer membrane vesicles (OMV), delivering bacterial substances including the oncogenic cytotoxin-associated CagA protein to their surroundings. We investigated the effects of H. pylori OMV carrying CagA (OMV-CagA) on cell junctions and ATP-binding proteome of epithelial monolayers, using proteomics, mass spectrometry and imaging. OMV-CagA localized in close vicinity of ZO-1 tight junction protein and induced histone H1 binding to ATP. We suggest the expression of novel events in the interactions between H. pylori OMV and epithelia, which may have an influence on host gene transcription and lead to different outcomes of an infection and development of cancer.

Place, publisher, year, edition, pages
Oxford University Press (OUP): Policy B - Oxford Open Option D, 2015
Keywords
Helicobacter pylori; outer membrane vesicles; CagA; ATP-proteome; histone H1; epithelial cell-cell junctions
National Category
Clinical Medicine
Identifiers
urn:nbn:se:liu:diva-120236 (URN)10.1093/femsle/fnv076 (DOI)000356890900007 ()25956174 (PubMedID)
Note

Funding Agencies|Swedish Research Council [2005-4636, 2008-3000, 2014-4361, 2007-3483, 2010-3045]; Euro-BioImaging; Magnus Bergvalls Foundation; Faculty of Health Sciences, Linkoping University; Kempe Memorial Foundation; Cancerfonden [CAN 2012/759]; Cancerforskningsfonden i Norrland; Insamlingstiftelsen for medicinsk forskning vid Umea universitet

Available from: 2015-07-21 Created: 2015-07-20 Last updated: 2017-12-04
Ingelsson, B., Fristedt, R. & Turkina, M. (2015). Phosphorylation stoichiometry determination in plant photosynthetic membranes.. In: Waltraud X. Schulze (Ed.), Plant Phosphoproteomics: Methods and Protocols (pp. 121-134). New York: Springer-Verlag New York
Open this publication in new window or tab >>Phosphorylation stoichiometry determination in plant photosynthetic membranes.
2015 (English)In: Plant Phosphoproteomics: Methods and Protocols / [ed] Waltraud X. Schulze, New York: Springer-Verlag New York, 2015, p. 121-134Chapter in book (Refereed)
Abstract [en]

This chapter describes different strategies for the study of phosphorylation dynamics and stoichiometry in photosynthetic membranes. Detailed procedures for the detection, large-scale identification, and quantification of phosphorylated proteins optimized for plant thylakoid proteins are given.

Place, publisher, year, edition, pages
New York: Springer-Verlag New York, 2015
Series
Methods in Molecular Biology, ISSN 1064-3745 ; 1306
Keywords
Protein phosphorylation, Immunological detection, Phosphorylation stoichiometry, Mass spectrometry
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:liu:diva-125278 (URN)10.1007/978-1-4939-2648-0_9 (DOI)978-1-4939-2647-3 (ISBN)978-1-4939-2648-0 (ISBN)
Available from: 2016-02-19 Created: 2016-02-19 Last updated: 2016-03-01Bibliographically approved
Ljunggren, S., Levels, J. H., Turkina, M. V., Sundberg, S., Bochem, A. E., Hovingh, K., . . . Karlsson, H. (2014). ApoA-I mutations, L202P and K131del, in HDL from heterozygotes with low HDL-C. PROTEOMICS - Clinical Applications, 8(3-4), 241-250
Open this publication in new window or tab >>ApoA-I mutations, L202P and K131del, in HDL from heterozygotes with low HDL-C
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2014 (English)In: PROTEOMICS - Clinical Applications, ISSN 1862-8346, E-ISSN 1862-8354, Vol. 8, no 3-4, p. 241-250Article in journal (Refereed) Published
Abstract [en]

PURPOSE: Mutations in apolipoprotein A-I (apoA-I) may affect plasma high-density lipoprotein (HDL) cholesterol levels and the risk for cardiovascular disease but little is known about the presence and effects of circulating apoA-I variants. This study investigates whether the apoA-I mutations, apoA-I(L202P) and apoA-I(K131del) , are present on plasma HDL particles derived from heterozygote carriers and whether this is associated to changes in HDL protein composition.

EXPERIMENTAL DESIGN: Plasma HDL of heterozygotes for either apoA-I(L202P) or apoA-I(K131del) and family controls was isolated using ultracentrifugation. HDL proteins were separated by 2DE and analyzed by MS.

RESULTS: ApoA-I peptides containing apoA-I(L202P) or apoA-I(K131del) were identified in HDL from heterozygotes. The apoA-I(L202P) mutant peptide was less abundant than wild-type peptide while the apoA-I(K131del) mutant peptide was more abundant than wild-type peptide in the heterozygotes. Two-dimensional gel electrophoresis analyses indicated that, compared to controls, HDL in apoA-I(L202P) carriers contained less apoE and more zinc-α-2-glycoprotein while HDL from the apoA-I(K131del) heterozygotes contained more alpha-1-antitrypsin and transthyretin.

CONCLUSIONS AND CLINICAL RELEVANCE: Both apoA-I(L202P) and apoA-I(K131del) were identified in HDL. In heterozygotes, these mutations have markedly differential effects on the concentration of wild-type apoA-I in the circulation, as well as the HDL proteome, both of which might affect the clinical phenotype encountered in the heterozygous carriers.

Place, publisher, year, edition, pages
Wiley-Blackwell, 2014
National Category
Cell and Molecular Biology Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:liu:diva-105992 (URN)10.1002/prca.201300014 (DOI)000334251600013 ()24273187 (PubMedID)
Available from: 2014-04-16 Created: 2014-04-16 Last updated: 2018-01-11
Ghafouri, N., Ghafouri, B., Fowler, C. J., Larsson, B., Turkina, M., Karlsson, L. & Gerdle, B. (2014). Effects of Two Different Specific Neck Exercise Interventions on Palmitoylethanolamide and Stearoylethanolamide Concentrations in the Interstitium of the Trapezius Muscle in Women with Chronic Neck Shoulder Pain. Pain medicine (Malden, Mass.), 15(8), 1379-1389
Open this publication in new window or tab >>Effects of Two Different Specific Neck Exercise Interventions on Palmitoylethanolamide and Stearoylethanolamide Concentrations in the Interstitium of the Trapezius Muscle in Women with Chronic Neck Shoulder Pain
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2014 (English)In: Pain medicine (Malden, Mass.), ISSN 1526-2375, E-ISSN 1526-4637, Vol. 15, no 8, p. 1379-1389Article in journal (Refereed) Published
Abstract [en]

Purpose. Chronic neck/shoulder pain (CNSP) is one of the most common pain conditions. The understanding of mechanisms, including the peripheral balance between nociceptive and antinociceptive processes, is incomplete. N-acylethanolamines (NAEs) are a class of endogenous compounds that regulate inflammation and pain. The aim of this study was to investigate the levels of two NAEs: the peroxisome proliferator-activated receptor type-a ligand palmitoylethanolamide (PEA) and stearoylethanolamide (SEA) in the muscle interstitium of the trapezius muscle in women with CNSP randomized to two different neck specific training programs and in a healthy pain-free control group (CON). Materials and Methods. Fifty-seven women with CNSP were randomized to strength + stretch or stretch alone exercise programs. Twenty-nine subjects underwent microdialysis procedure before and after 4-6 months of exercise. Twenty-four CON subjects underwent microdialysis procedure before and after 4-6 months without any intervention in between. Microdialysate samples were collected from the trapezius muscle and analyzed by mass spectrometry for PEA and SEA levels. Results. PEA and SEA levels were significantly higher in CNSP patients compared with CON. PEA was significantly higher in CNSP than in CON after both training programs. SEA was significantly higher in CNSP than in CON after stretch alone but not after strength + stretch training. A significant positive correlation was found between changes in pain intensity and in SEA levels in the strength + stretch group, but not in the stretch alone group. Conclusion. Our results indicate that exercise interventions differentially affect the levels of the bioactive lipids PEA and SEA in the interstitium of the trapezius muscle in women with CNSP.

Place, publisher, year, edition, pages
Wiley-Blackwell, 2014
Keywords
N-Acylethanolamines; Palmitoylethanolamide; Stearoylethanolamide; Exercise; Chronic Muscle Pain; Microdialysis
National Category
Basic Medicine Clinical Medicine
Identifiers
urn:nbn:se:liu:diva-112070 (URN)10.1111/pme.12486 (DOI)000342630800022 ()24995488 (PubMedID)
Note

Funding Agencies|Swedish Council for Working Life and Social Research; Swedish Research Council; PAINOMICS(TM) laboratory of Linkoping University

Available from: 2014-11-14 Created: 2014-11-13 Last updated: 2018-01-11Bibliographically approved
Bagheri, M., Rezakhani, A., Nyström, S., Turkina, M., Roghani, M., Hammarström, P. & Mohseni, S. (2013). Amyloid Beta1-40-Induced Astrogliosis and the Effect of Genistein Treatment in Rat: A Three-Dimensional Confocal Morphometric and Proteomic Study. PLoS ONE, 8(10)
Open this publication in new window or tab >>Amyloid Beta1-40-Induced Astrogliosis and the Effect of Genistein Treatment in Rat: A Three-Dimensional Confocal Morphometric and Proteomic Study
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2013 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 8, no 10Article in journal (Refereed) Published
Abstract [en]

Astrocytes are highly involved in regulation and homeostasis of the extracellular environment in the healthy brain. In pathological conditions, these cells play a major role in the inflammatory response seen in CNS tissues, which is called reactive astrogliosis and includes hypertrophy and proliferation of astrocytes. Here, we performed 3D confocal microscopy to evaluate the morphological response of reactive astrocytes positive for glial fibrillary acidic protein (GFAP) in rats, to the presence of Aβ1–40 in the rat brain before and after treatment with genistein. In 50 astrocytes per animal, we measured the volume and surface area for the nucleus, cell body, the entire cell, the tissue covered by single astrocytes and quantified the number and length of branches, the density of the astrocytes and the intensity of GFAP immunoreactivity. Injecting Aβ1–40 into the brain of rats caused astrogliosis indicated by increased values for all measured parameters. Mass spectrometric analysis of hippocampal tissue in Aβ1–40-injected brain showed decreased amounts of tubulins, enolases and myelin basic protein, and increased amounts of dihydropyrimidinase-related protein 2. In Aβ1–40-injected rats pretreated with genistein, GFAP intensity was decreased to the sham-operated group level, and Aβ1–40-induced astrogliosis was significantly ameliorated.

Place, publisher, year, edition, pages
Public Library of Science, 2013
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-101388 (URN)10.1371/journal.pone.0076526 (DOI)000325810900075 ()
Note

Funding Agencies|County Council of Ostergotland (Sweden)||Linkoping University||Cellular and Molecular Research Center||Tehran University of Medical Sciences (Tehran, Iran)||

Available from: 2013-11-22 Created: 2013-11-21 Last updated: 2019-11-08
Rohini Rajan, M., Fagerholm, S., Jonsson, C., Kjölhede, P., Turkina, M. & Strålfors, P. (2013). Phosphorylation of IRS1 at Serine 307 in Response to Insulin in Human Adipocytes Is Not Likely to be Catalyzed by p70 Ribosomal S6 Kinase. PLoS ONE, 8(4)
Open this publication in new window or tab >>Phosphorylation of IRS1 at Serine 307 in Response to Insulin in Human Adipocytes Is Not Likely to be Catalyzed by p70 Ribosomal S6 Kinase
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2013 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 8, no 4Article in journal (Refereed) Published
Abstract [en]

The insulin receptor substrate-1 (IRS1) is phosphorylated on serine 307 (human sequence, corresponding to murine serine 302) in response to insulin as part of a feedback loop that controls IRS1 phosphorylation on tyrosine residues by the insulin receptor. This in turn directly affects downstream signaling and is in human adipocytes implicated in the pathogenesis of insulin resistance and type 2 diabetes. The phosphorylation is inhibited by rapamycin, a specific inhibitor of mammalian target of rapamycin (mTOR) in complex with raptor (mTORC1). The mTORC1-downstream p70 ribosomal protein S6 kinase (S6K1), which is activated by insulin, can phosphorylate IRS1 at serine 307 in vitro and is considered the physiological protein kinase. Because the IRS1 serine 307-kinase catalyzes a critical step in the control of insulin signaling and constitutes a potential target for treatment of insulin resistance, it is important to know whether S6K1 is the physiological serine 307-kinase or not. We report that, by several criteria, S6K1 does not phosphorylate IRS1 at serine 307 in response to insulin in intact human primary adipocytes: (i) The time-courses for phosphorylation of S6K1 and its phosphorylation of S6 are not compatible with the phosphorylation of IRS1 at serine 307; (ii) A dominant-negative construct of S6K1 inhibits the phosphorylation of S6, without effect on the phosphorylation of IRS1 at serine 307; (iii) The specific inhibitor of S6K1 PF-4708671 inhibits the phosphorylation of S6, without effect on phosphorylation of IRS1 at serine 307. mTOR-immunoprecipitates from insulin-stimulated adipocytes contains an unidentified protein kinase specific for phosphorylation of IRS1 at serine 307, but it is not mTOR or S6K1.

Place, publisher, year, edition, pages
Public Library of Science, 2013
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-93257 (URN)10.1371/journal.pone.0059725 (DOI)000317717300032 ()
Note

Funding Agencies|Swedish Diabetes Fund||Novo Nordic Foundation||University of Linkoping||Swedish Research Council||

Available from: 2013-05-28 Created: 2013-05-28 Last updated: 2019-06-28
Herbstova, M., Tietz, S., Kinzel, C., Turkina, M. & Kirchhoff, H. (2012). Architectural switch in plant photosynthetic membranes induced by light stress. Proceedings of the National Academy of Sciences of the United States of America, 109(49), 20130-20135
Open this publication in new window or tab >>Architectural switch in plant photosynthetic membranes induced by light stress
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2012 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 109, no 49, p. 20130-20135Article in journal (Refereed) Published
Abstract [en]

Unavoidable side reactions of photosynthetic energy conversion can damage the water-splitting photosystem II (PSII) holocomplex embedded in the thylakoid membrane system inside chloroplasts. Plant survival is crucially dependent on an efficient molecular repair of damaged PSII realized by a multistep repair cycle. The PSII repair cycle requires a brisk lateral protein traffic between stacked grana thylakoids and unstacked stroma lamellae that is challenged by the tight stacking and low protein mobility in grana. We demonstrated that high light stress induced two main structural changes that work synergistically to improve the accessibility between damaged PSII in grana and its repair machinery in stroma lamellae: lateral shrinkage of grana diameter and increased protein mobility in grana thylakoids. It follows that high light stress triggers an architectural switch of the thylakoid network that is advantageous for swift protein repair. Studies of the thylakoid kinase mutant stn8 and the double mutant stn7/8 demonstrate the central role of protein phosphorylation for the structural alterations. These findings are based on the elaboration of mathematical tools for analyzing confocal laser-scanning microscopic images to study changes in the sophisticated thylakoid architecture in intact protoplasts.

Place, publisher, year, edition, pages
National Academy of Sciences; 1999, 2012
Keywords
confocal microscopy, macromolecular crowding, photosynthesis
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-86891 (URN)10.1073/pnas.1214265109 (DOI)000312347200057 ()
Note

Funding Agencies|Washington State Agricultural Research Center||National Science Foundation|MCB-1158571|United States-Israel Binational Agricultural Research and Development Fund|US-4334-10|

Available from: 2013-01-07 Created: 2013-01-07 Last updated: 2017-12-06
Karlsson, T., Turkina, M., Yakymenko, O., Magnusson, K.-E. & Vikström, E. (2012). The Pseudomonas aeruginosa N-Acylhomoserine Lactone Quorum Sensing Molecules Target IQGAP1 and Modulate Epithelial Cell Migration. PLOS PATHOGENS, 8(10)
Open this publication in new window or tab >>The Pseudomonas aeruginosa N-Acylhomoserine Lactone Quorum Sensing Molecules Target IQGAP1 and Modulate Epithelial Cell Migration
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2012 (English)In: PLOS PATHOGENS, ISSN 1553-7374, Vol. 8, no 10Article in journal (Refereed) Published
Abstract [en]

Quorum sensing (QS) signaling allows bacteria to control gene expression once a critical population density is achieved. The Gram-negative human pathogen Pseudomonas aeruginosa uses N-acylhomoserine lactones (AHL) as QS signals, which coordinate the production of virulence factors and biofilms. These bacterial signals can also modulate human cell behavior. Little is known about the mechanisms of the action of AHL on their eukaryotic targets. Here, we found that N-3-oxododecanoyl- L-homoserine lactone 3O-C-12-HSL modulates human intestinal epithelial Caco-2 cell migration in a dose- and time-dependent manner. Using new 3O-C-12-HSL biotin and fluorescently-tagged probes for LC-MS/MS and confocal imaging, respectively, we demonstrated for the first time that 3O-C-12-HSL interacts and co-localizes with the IQ-motif-containing GTPase-activating protein IQGAP1 in Caco-2 cells. The interaction between IQGAP1 and 3O-C-12-HSL was further confirmed by pull-down assay using a GST-tagged protein with subsequent Western blot of IQGAP1 and by identifying 3O-C-12-HSL with a sensor bioassay. Moreover, 3O-C-12-HSL induced changes in the phosphorylation status of Rac1 and Cdc42 and the localization of IQGAP1 as evidenced by confocal and STED microscopy and Western blots. Our findings suggest that the IQGAP1 is a novel partner for P. aeruginosa 3O-C-12-HSL and likely the integrator of Rac1 and Cdc42- dependent altered cell migration. We propose that the targeting of IQGAP1 by 3O-C-12-HSL can trigger essential changes in the cytoskeleton network and be an essential component in bacterial - human cell communication.

Place, publisher, year, edition, pages
Public Library of Science, 2012
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-86387 (URN)10.1371/journal.ppat.1002953 (DOI)000310530300018 ()
Note

Funding Agencies|Swedish Research Council||European Science foundation||TraPPs Euromembrane project||King Gustaf V 80-Year Foundation||Faculty of Health Sciences, Linkoping University||

Available from: 2012-12-14 Created: 2012-12-14 Last updated: 2013-01-14
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