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Andersson, Jens
Alternative names
Publications (10 of 20) Show all publications
Magnusson, K., Appelqvist, H., Cieslar-Pobuda, A., Wigenius, J., Karlsson, T., Los, M. J., . . . Nilsson, P. (2015). Differential vital staining of normal fibroblasts and melanoma cells by an anionic conjugated polyelectrolyte. Cytometry Part A, 87(3), 262-272
Open this publication in new window or tab >>Differential vital staining of normal fibroblasts and melanoma cells by an anionic conjugated polyelectrolyte
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2015 (English)In: Cytometry Part A, ISSN 1552-4922, E-ISSN 1552-4930, Vol. 87, no 3, p. 262-272Article in journal (Refereed) Published
Abstract [en]

Molecular probes for imaging of live cells are of great interest for studying biological and pathological processes. The anionic luminescent conjugated polythiophene (LCP) polythiophene acetic acid (PTAA), has previously been used for vital staining of cultured fibroblasts as well as transformed cells with results indicating differential staining due to cell phenotype. Herein, we investigated the behavior of PTAA in two normal and five transformed cells lines. PTAA fluorescence in normal cells appeared in a peripheral punctated pattern whereas the probe was more concentrated in a one-sided perinuclear localization in the five transformed cell lines. In fibroblasts, PTAA fluorescence was initially associated with fibronectin and after 24 h partially localized to lysosomes. The uptake and intracellular target in malignant melanoma cells was more ambiguous and the intracellular target of PTAA in melanoma cells is still elusive. PTAA was well tolerated by both fibroblasts and melanoma cells, and microscopic analysis as well as viability assays showed no signs of negative influence on growth. Stained cells maintained their proliferation rate for at least 12 generations. Although the probe itself was nontoxic, photoinduced cellular toxicity was observed in both cell lines upon irradiation directly after staining. However, no cytotoxicity was detected when the cells were irradiated 24 h after staining, indicating that the photoinduced toxicity is dependent on the cellular location of the probe. Overall, these studies certified PTAA as a useful agent for vital staining of cells, and that PTAA can potentially be used to study cancer-related biological and pathological processes.

Place, publisher, year, edition, pages
Wiley: 12 months, 2015
Keywords
Conjugated polyelectrolyte; Fibroblast; Fluorescence; Luminescent conjugated polythiophene; Melanoma; Photoinduced toxicity
National Category
Structural Biology Cell and Molecular Biology
Identifiers
urn:nbn:se:liu:diva-115887 (URN)10.1002/cyto.a.22627 (DOI)000349984200009 ()25605326 (PubMedID)2-s2.0-84923259526 (Scopus ID)
Available from: 2015-03-23 Created: 2015-03-23 Last updated: 2018-09-14
Hevekerl, H., Wigenius, J., Persson, G., Inganäs, O. & Widengren, J. (2014). Dark States in Ionic Oligothiophene Bioprobes-Evidence from Fluorescence Correlation Spectroscopy and Dynamic Light Scattering. Journal of Physical Chemistry B, 118(22), 5924-5933
Open this publication in new window or tab >>Dark States in Ionic Oligothiophene Bioprobes-Evidence from Fluorescence Correlation Spectroscopy and Dynamic Light Scattering
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2014 (English)In: Journal of Physical Chemistry B, ISSN 1520-6106, E-ISSN 1520-5207, Vol. 118, no 22, p. 5924-5933Article in journal (Refereed) Published
Abstract [en]

Luminescent conjugated polyelectrolytes (LCPs) can upon interaction with biological macromolecules change their luminescent properties, and thereby serve as conformation- and interaction-sensitive biomolecular probes. However, to exploit this in a more quantitative manner, there is a need to better understand the photophysical processes involved. We report studies of the conjugated pentameric oligothiophene, derivative p-FTAA, which changes optical properties with different p-FTAA concentrations in aqueous buffers, and in a pH and oxygen saturation dependent manner. Using dynamic light scattering, luminescence spectroscopy and fluorescence correlation spectroscopy, we find evidence for a monomer dimer equilibrium, for the formation of large clusters of p-FTAA in aqueous environment, and can couple aggregation to changed emission properties of oligothiophenes. In addition, we observe the presence of at least two dark transient states, one presumably being a triplet state. Oxygen was found to statically quench the p-FTAA fluorescence but also to promote molecular fluorescence by quenching dark transient states of the p-FTAA molecules. Taken together, this study provides knowledge of fluorescence and photophysical features essential for applying p-FTAA and other oligothiophene derivatives for diagnostic purposes, including detection and staining of amyloid aggregates.

Place, publisher, year, edition, pages
American Chemical Society, 2014
National Category
Engineering and Technology
Identifiers
urn:nbn:se:liu:diva-108795 (URN)10.1021/jp501324e (DOI)000337011400013 ()
Available from: 2014-07-07 Created: 2014-07-06 Last updated: 2017-12-05
Wigenius, J. A., Persson, G., Widengren, J. & Inganäs, O. (2011). Interactions between a luminescent conjugated oligoelectrolyte and insulin during early phases of amyloid formation. Macromolecular Bioscience, 11(8), 1120-1127
Open this publication in new window or tab >>Interactions between a luminescent conjugated oligoelectrolyte and insulin during early phases of amyloid formation
2011 (English)In: Macromolecular Bioscience, ISSN 1616-5187, E-ISSN 1616-5195, Vol. 11, no 8, p. 1120-1127Article in journal (Refereed) Published
Abstract [en]

Folding of an amino acid polypeptide chain into its native three-dimensional protein is a delicate process. Misfolding may cause assembly of dysfunctional proteins leading to aggregated assemblies, in medicine denoted amyloids, causing Alzheimer’s, Parkinson and a number of other protein related diseases. Amyloids have also shown promising results as building blocks in organic electronic applications, associated to conjugated polymers. Luminescent conjugated oligo- and polythiophenes (LCPs) have been further developed for biosensor applications exhibiting good ability to discriminate and determine different types of amyloid enrichment in complex environments, such as in tissue sections. The nature of interaction between the amyloid assemblies and LCPs is still not fully understood. In this study we use steady-state fluorescence spectroscopy, dynamic light scattering, transmission electron microscopy and fluorescence correlation spectroscopy to follow the interplay between the anionic oligothiophene derivative 4',3'''-Bis-carboxymethyl-[2,2';5',2'';5'',2''';5''',2'''']quinque thiophene-5,5''''-dicarboxylic acid (p-FTAA), and prefibrillar protein assemblies present during the earlier stage of in vitro fibrillation of bovine insulin. Our findings confirm that p-FTAA interacts with pre-fibrillar species of insulin preceding the formation of mature insulin amyloid fibrils, and insights regarding the molecular interplay between p-FTAA and these species are provided.

Place, publisher, year, edition, pages
Wiley, 2011
National Category
Natural Sciences
Identifiers
urn:nbn:se:liu:diva-54898 (URN)10.1002/mabi.201100016 (DOI)000294160900011 ()
Note
Funding Agencies|Swedish Science Council (VR)||Strategic Research Foundation (SSF) through the center for organic bioelectronics (OBOE)||Knut and Alice Wallenberg foundation||Available from: 2010-04-20 Created: 2010-04-20 Last updated: 2017-12-12
Wigenius, J. (2010). Conjugated Polyelectrolytes in Interactions with Biomolecules for Supramolecular assembly and Sensing. (Doctoral dissertation). Linköping: Linköping University Electronic Press
Open this publication in new window or tab >>Conjugated Polyelectrolytes in Interactions with Biomolecules for Supramolecular assembly and Sensing
2010 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Conjugated polyelectrolytes (CP) show interesting electrical and optical properties for organic electronics as well as for life science applications. Their possibilities of supramolecular assembly with nanowire like misfolded proteins, amyloids, as well as synthetic polypeptides or DNA forming conducting or luminescent nano composites is highly interesting as being a truly bottom up approach for fabrication of OLEDs, photovoltaic’s as well as logic devices. The conformation and aggregation dependent luminescence properties from the special class of CPs, Luminescent conjugated polyelectrolytes (LCP), have been utilised and developed as sensors to follow and study biomolecular interactions, DNA hybridisation, protein-protein interactions and staining of living cell cultures and tissue slides. In this thesis we are bringing the evolution a few steps further by applying new types of experimental techniques, such as light scattering and fluorescence correlation spectroscopy, combined with standard techniques as soft lithography and different spectroscopy techniques, to gain better knowledge of the optical behaviour of LCPs and their interactions with biomolecules. We explore the optical properties and vibronic transitions of LCPs; their ability of resonance energy transfer with LCPs indicating super lightning behaviour; the opposite fluorescence shift when interacting with α-helical rich polypeptides compared to earlier reports of interactions upon staining of β-rich amyloids; and the possibility of LCPs to influence protein aggregation as well as the possibility of fabricating biochips based on LCPs and soft lithography. Here we also show fundamental limitations to patterning using macromolecular fluids, of general relevance to soft lithography and nanoimprint lithography with low viscosity polymers.

Place, publisher, year, edition, pages
Linköping: Linköping University Electronic Press, 2010. p. 60
Series
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 1308
National Category
Natural Sciences
Identifiers
urn:nbn:se:liu:diva-54902 (URN)978-91-7393-408-4 (ISBN)
Public defence
2010-05-14, Planck, Fysikhuset, Campus Valla, Linköpings universitet, Linköping, 14:00 (English)
Opponent
Supervisors
Available from: 2010-04-20 Created: 2010-04-20 Last updated: 2014-04-08Bibliographically approved
Wigenius, J., Magnusson, K., Björk, P., Andersson, O. & Inganäs, O. (2010). DNA Chips with Conjugated Polyelectrolytes in Resonance Energy Transfer Mode. LANGMUIR, 26(5), 3753-3759
Open this publication in new window or tab >>DNA Chips with Conjugated Polyelectrolytes in Resonance Energy Transfer Mode
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2010 (English)In: LANGMUIR, ISSN 0743-7463, Vol. 26, no 5, p. 3753-3759Article in journal (Refereed) Published
Abstract [en]

We show how to use well-defined conjugated polyelectrolytes (CPEs) combined With Surface energy patterning to Fabricate DNA Chips utilizing A fluorescence signal amplification. Cholesterol-modified DNA strands in complex with it CPE are adsorbed to a surface energy pattern, formed by printing with soft elastomer stamps. Hybridization of the surface bound DNA strands with it short complementary strand from Solution is monitored using both fluorescence microscopy and imaging surface plasmon resonance. The CPEs act as antennas, enhancing resonance energy transfer to the dye-labeled DNA when complementary hybridization of the double strand occurs.

National Category
Engineering and Technology
Identifiers
urn:nbn:se:liu:diva-54255 (URN)10.1021/la903101v (DOI)000274636900113 ()
Available from: 2010-03-05 Created: 2010-03-05 Last updated: 2015-05-29
Hamedi, M., Wigenius, J., Tai, F.-i., Björk, P. & Aili, D. (2010). Polypeptide-guided assembly of conducting polymer nanocomposites. NANOSCALE, 2(10), 2058-2061
Open this publication in new window or tab >>Polypeptide-guided assembly of conducting polymer nanocomposites
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2010 (English)In: NANOSCALE, ISSN 2040-3364, Vol. 2, no 10, p. 2058-2061Article in journal (Refereed) Published
Abstract [en]

A strategy for fabrication of electroactive nanocomposites with nanoscale organization, based on self-assembly, is reported. Gold nanoparticles are assembled by a polypeptide folding-dependent bridging. The polypeptides are further utilized to recruit and associate with a water soluble conducting polymer. The polymer is homogenously incorporated into the nanocomposite, forming conducting pathways which make the composite material highly conducting.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2010
National Category
Engineering and Technology
Identifiers
urn:nbn:se:liu:diva-61181 (URN)10.1039/c0nr00299b (DOI)000282686200019 ()
Note
Original Publication: Mahiar Hamedi, Jens Wigenius, Feng-i Tai, Per Björk and Daniel Aili, Polypeptide-guided assembly of conducting polymer nanocomposites, 2010, NANOSCALE, (2), 10, 2058-2061. http://dx.doi.org/10.1039/c0nr00299b Copyright: Royal Society of Chemistry http://www.rsc.org/ Available from: 2010-11-05 Created: 2010-11-05 Last updated: 2014-10-08Bibliographically approved
Wigenius, J., Björk, P., Hamedi, M. & Aili, D. (2010). Supramolecular Assembly of Designed α-Helical Polypeptide-Based Nanostructures and Luminescent Conjugated Polyelectrolytes. Macromolecular Bioscience, 10(8), 836-841
Open this publication in new window or tab >>Supramolecular Assembly of Designed α-Helical Polypeptide-Based Nanostructures and Luminescent Conjugated Polyelectrolytes
2010 (English)In: Macromolecular Bioscience, ISSN 1616-5187, E-ISSN 1616-5195, Vol. 10, no 8, p. 836-841Article in journal (Refereed) Published
Abstract [en]

Designed polypeptides with controllable folding properties are utilized as supramolecular templates for fabrication of ordered nanoscale molecular and fibrous assemblies of luminescent conjugated polymers (LCPs). The properties of the LCPs as well as the three dimensional conformation of the polypeptide-scaffold determine how the polymers are arranged in the supramolecular construct, which highly affects the properties of the hybrid material. The ability to control the polypeptide conformation and assembly into fibers provide a promising route for tuning the optical properties of LCPs and for fabrication of complex functional supramolecules with well defined structural properties.

Place, publisher, year, edition, pages
John Wiley & Sons, 2010
Keywords
α-helical; conjugated polymers; hybrid materials; peptides; self-assembly
National Category
Natural Sciences
Identifiers
urn:nbn:se:liu:diva-54899 (URN)10.1002/mabi.200900463 (DOI)000281387900002 ()
Available from: 2010-04-20 Created: 2010-04-20 Last updated: 2017-12-12Bibliographically approved
Hamedi, M., Wigenius, J., Tai, F.-I., Björk, P. & Aili, D. (2010). Synthetic Polypeptides as Scaffolds for Supramolecular Assembly of Conducting Polymer Nanocomposites.
Open this publication in new window or tab >>Synthetic Polypeptides as Scaffolds for Supramolecular Assembly of Conducting Polymer Nanocomposites
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2010 (English)Manuscript (preprint) (Other academic)
Abstract [en]

The development of nanoelectronics has resulted in enormous advancements in fabrication techniques that have enabled massproduction of CMOS circuits with feature sizes below 45nm. There is a large interest in new methods to further push the size limits, lower the production costs and to facilitate the design of more advanced three-dimensional structures beyond today’s 2.5 dimensional architectures. Self-assembly is probably the most important scheme in this development and is currently applied to many different areas and classes of nanoelectronics. Self-assembly enables fabrication of structures well below 10 nm in feature size and allows for incorporation of novel nanomaterials, such as metallic and semiconducting nanoparticles with many interesting optical and electrical properties. The controlled self-assembly of electro-active nanocomposites is of great interest for the development of novel functional materials including biosensors, electrochromic/plasmonic hybrid devices, and polymer/nanoparticle-based memories.

Keywords
Conducting Polymers, Organic Electronics, Conducting Nanowires, Self- Assembly, Supramolecular Materials
National Category
Natural Sciences
Identifiers
urn:nbn:se:liu:diva-54901 (URN)
Available from: 2010-04-20 Created: 2010-04-20 Last updated: 2010-04-20
Karlsson, R., Herland, A., Hamedi, M., Wigenius, J., Åslund, A., Liu, X., . . . Konradsson, P. (2009). Iron-Catalyzed Polymerization of Alkoxysulfonate-Functionalized 3,4-Ethylenedioxythiophene Gives Water-Soluble Poly(3,4-ethylenedioxythiophene) of High Conductivity. Chemistry of Materials, 21(9), 1815-1821
Open this publication in new window or tab >>Iron-Catalyzed Polymerization of Alkoxysulfonate-Functionalized 3,4-Ethylenedioxythiophene Gives Water-Soluble Poly(3,4-ethylenedioxythiophene) of High Conductivity
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2009 (English)In: Chemistry of Materials, ISSN 0897-4756, E-ISSN 1520-5002, Vol. 21, no 9, p. 1815-1821Article in journal (Refereed) Published
Abstract [en]

Chemical polymerization of a 3,4-ethylenedioxythiophene derivative bearing a sulfonate group (EDOTS) is reported. The polymer, PEDOT-S, is fully water-soluble and has been produced by polymerizing EDOT-S in water, using Na2S2O8 and a catalytic amount of FeCl3. Elemental analysis and XPS measurements indicate that PEDOT-S is a material with a substantial degree of self-doping, but also contains free sulfate ions as charge-balancing counterions of the oxidized polymer. Apart from self-doping PEDOT-S, the side chains enable full water solubility of the material; DLS studies show an average cluster size of only 2 nm. Importantly, the solvation properties of the PEDOT-S are reflected in spin-coated films, which show a surface roughness of 1.2 nm and good conductivity (12 S/cm) in ambient conditions. The electro-optical properties of this material are shown with cyclic voltammetry and spectroelectrochemical experiment reveals an electrochromic contrast (similar to 48% at lambda(max) = 606 nm).

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2009
National Category
Natural Sciences
Identifiers
urn:nbn:se:liu:diva-18398 (URN)10.1021/cm801512r (DOI)000265781000012 ()
Available from: 2009-05-25 Created: 2009-05-25 Last updated: 2017-12-13Bibliographically approved
Björk, P., Thomsson, D., Mirzov, O., Andersson, J., Inganäs, O. & Scheblykin , I. G. (2009). Oligothiophene Assemblies Defined by DNA Interaction: From Single Chains to Disordered Clusters. SMALL, 5(1), 96-103
Open this publication in new window or tab >>Oligothiophene Assemblies Defined by DNA Interaction: From Single Chains to Disordered Clusters
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2009 (English)In: SMALL, ISSN 1613-6810 , Vol. 5, no 1, p. 96-103Article in journal (Refereed) Published
Abstract [en]

The organization of conjugated polyelectrolytes (CPEs) interacting with biomolecules sets conditions for the biodetection of biological processes and identity, through the use of optical emission from the CPE. Herein, a well-defined CPE and its binding to DNA is studied. By using dynamic light scattering and circular dichroism spectroscopy, it is shown that the CPE forms a multimolecule ensemble in aqueous solution that is more than doubled it? size when interacting with a small DNA chain, while single chains are evident in ethanol. The related changes in the fluorescence spectra upon polymer aggregation are assigned to oscillator strength redistribution between vibronic transitions in weakly coupled H-aggregates. An enhanced single-molecule spectroscopy technique that allows full control of excitation and emission light polarization is applied to combed and decorated;,DNA chains. It is found that the organization of combed CPE-lambda DNA complexes (when dry on the surface) allows considerable variation of CPE distances and direction relative to the DNA chain. By analysis of the polarization data. energy transfer between the polymer chains in individual complexes is confirmed and their sizes estimated.

Keywords
aggregation, conjugated polymers, DNA, fluorescence, single-molecule spectroscopy
National Category
Natural Sciences
Identifiers
urn:nbn:se:liu:diva-16829 (URN)10.1002/smll.200800855 (DOI)
Available from: 2009-02-20 Created: 2009-02-20 Last updated: 2010-04-20
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