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Mårdh, Sven
Publications (10 of 20) Show all publications
Kuroda, T., Ito, M., Wada, Y., Kitadai, Y., Tanaka, S., Yoshida, K., . . . Chayama , K. (2008). Presence of Poorly Differentiated Component Correlated with Submucosal Invasion in the Early Diffuse-type Gastric Cancer. HEPATO-GASTROENTEROLOGY, 55(88), 2264-2268
Open this publication in new window or tab >>Presence of Poorly Differentiated Component Correlated with Submucosal Invasion in the Early Diffuse-type Gastric Cancer
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2008 (English)In: HEPATO-GASTROENTEROLOGY, ISSN 0172-6390 , Vol. 55, no 88, p. 2264-2268Article in journal (Refereed) Published
Abstract [en]

Background/Aims. Diffuse-type gastric carcinoma is associated with a poor prognosis. However, the clinical behavior of diffuse-type gastric cancer is not fully understood. The aim of this study is to distinguish the behaviors of early diffuse-type gastric carcinomas by sub classifying tumors according to their histologic features.

Methodology. A total of 114 cases of diffuse-type early gastric cancer were studied retrospectively. We analyzed and compared the resected cancer specimens according to the histologic components: as poorly differentiated adenocarcinoma component-present (poor+) versus poorly differentiated adenocarcinoma component-absent (poor-). Helicobacter pylori status was evaluated by Giemsa staining and IgG serology. We assessed the degree of cancer invasion and compared back-ground status of gastritis in accordance with the updated Sydney System criteria and serologic markers.

Results. In comparison to the poor+ cancers, the poor- cancers had a significantly larger portion of cells confined to the mucosa (p=0.002). Only 8 of the 114 cases were regarded as H. pylori-negative. Although we could not detect any serologic markers specific for gastritis with poor+ cancer, but the serum levels of gastrin was slightly higher in patients with poor+ cancers than in those with poor- cancers.

Conclusions. The biologic behavior of poor+ gastric carcinoma is worse than that of poor- carcinoma. There is a close relation between H. pylori infection and carcinogenesis of poorly differentiated adenocarcinoma.

Keywords
Gastric cancer, Helicobacter pylori, Gastritis, Diffuse type, Serologic markers
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-16866 (URN)
Available from: 2009-02-22 Created: 2009-02-20 Last updated: 2009-02-22
Borch, K., Skarsgard, J., Franzén, L., Mårdh, S. & Rehfeld, J. (2003). Benign gastric polyps - Morphological and functional origin. Digestive Diseases and Sciences, 48(7), 1292-1297
Open this publication in new window or tab >>Benign gastric polyps - Morphological and functional origin
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2003 (English)In: Digestive Diseases and Sciences, ISSN 0163-2116, E-ISSN 1573-2568, Vol. 48, no 7, p. 1292-1297Article in journal (Refereed) Published
Abstract [en]

The most common types of benign gastric polyps are fundic gland polyps, hyperplastic polyps, and adenomas. The aim of this study was to determine on which morphological and functional background benign gastric polyps develop. The study includes 85 consecutive patients with gastric polyps and sex and age-matched controls without polyps selected at random from a general population sample. The type of polyp was hyperplastic in 52 (61%), fundic gland in 18 (21%), adenoma in 10 (12%), carcinoid in 2 (2%), hamartoma in 2 ( 2%), and inflammatory fibroid in 1 (1%) of the cases. Routine biopsies from the gastric corpus and antrum were examined for presence of gastritis and H. pylori. Blood samples were analyzed for H. pylori antibodies, H+, K+-ATPase antibodies, gastrin, and pepsinogen I. Patients with hyperplastic polyps had increased P-gastrin concentrations and S-H+, K+-ATPase antibody titers and decreased S-pepsinogen I concentrations with a high prevalence of atrophic corpus gastritis or pangastritis. A similar pattern was observed among patients with adenomas, whereas patients with fundic gland polyps had normal serology and a lower prevalence of gastritis and H. pylori infection than controls. In conclusion, hyperplastic polyps and adenomas are generally associated with atrophic gastritis. Patients with fundic gland polyps seem to have a sounder mucosa than controls. Whereas the risk of malignant gastric neoplasia is increased in patients with hyperplastic polyps or adenomas, this does not seem to be the case in patients with fundic gland polyps.

Keywords
gastrin, gastritis, Helicobacter pylori, H+, K+-ATPase antibodies, morphology, pepsinogen I, polyps, stomach
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-48631 (URN)
Available from: 2009-10-11 Created: 2009-10-11 Last updated: 2017-12-12
Nägga, K., Rajani, R., Mårdh, E., Borch, K., Mårdh, S. & Marcusson, J. (2003). Cobalamin, folate, methylmalonic acid, homocysteine, and gastritis markers in dementia. Dementia and Geriatric Cognitive Disorders, 16(4), 269-275
Open this publication in new window or tab >>Cobalamin, folate, methylmalonic acid, homocysteine, and gastritis markers in dementia
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2003 (English)In: Dementia and Geriatric Cognitive Disorders, ISSN 1420-8008, E-ISSN 1421-9824, Vol. 16, no 4, p. 269-275Article in journal (Refereed) Published
Abstract [en]

The prevalence of dementia disorders, cobalamin and/or folate deficiency as well as gastritis increases with age. To investigate whether there is an association between these conditions, plasma homocysteine (Hcy), serum methylmalonic acid, serum cobalamin and blood folate concentrations were measured. Gastritis was indirectly diagnosed by measuring serum antibodies against H,K-ATPase, Helicobacter pylori and intrinsic factor, using enzyme-linked immunosorbent assays. The studied groups consisted of 47 patients with Alzheimer’s disease (AD), 9 with AD pathology in combination with additive vascular lesions, 59 with vascular dementia, 8 who were cognitively impaired, and 101 control cases. Plasma Hcy concentrations were significantly elevated in the dementia groups, with the highest levels in patients with vascular pathology. We conclude that hyperhomocysteinemia is a common finding in patients with dementia disorders of different etiologies. The markers for gastritis did not contribute to an elucidation of a possible connection between this condition, dementia disorders, or cobalamin/folate deficiency.

National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-24967 (URN)10.1159/000072812 (DOI)9378 (Local ID)9378 (Archive number)9378 (OAI)
Available from: 2009-10-07 Created: 2009-10-07 Last updated: 2019-06-27
Sundbom, M., Mårdh, E., Mårdh, S., Ohrvall, M. & Gustavsson, S. (2003). Reduction in serum pepsinogen I after Roux-en-Y gastric bypass. Journal of Gastrointestinal Surgery, 7(4), 529-535
Open this publication in new window or tab >>Reduction in serum pepsinogen I after Roux-en-Y gastric bypass
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2003 (English)In: Journal of Gastrointestinal Surgery, ISSN 1091-255X, E-ISSN 1873-4626, Vol. 7, no 4, p. 529-535Article in journal (Refereed) Published
Abstract [en]

The excluded stomach after Roux-en-Y gastric bypass (RYGBP) cannot be readily examined by endoscopy for obvious anatomic reasons. Thus it is difficult to monitor possible changes in the gastric mucosa. However, the type and severity of gastritis can now be assessed by a combination of serologic tests: pepsinogen I and antibodies to Helicobacter pylori and H,K-ATPase. Morbidly obese patients were examined before and 1 to 4 years after surgery. A group of 34 patients (mean age 39 years, BMI 44 kg/m2) underwent RYGBP, another group of 30 patients (mean age 42 years, BMI 44 kg/m2) had simple gastric restriction and served as control subjects. All patients, except one in the control group, had normal titers of pepsinogen I before surgery. One year after RYGBP, pepsinogen I levels were significantly reduced, as compared to the control group (P<0.0001), and remained low throughout the study. The control group had stable pepsinogen I levels. In both groups, few patients had increased titers of H. pylori or H,K-ATPase antibodies, but these abnormalities remained unchanged. Low pepsinogen I levels, similar to those we observed in our RYGBP patients, have been linked to chronic atrophic gastritis. However, the absence of food stimulation in the excluded stomach could also be a reason for the low pepsinogen I levels. © 2003 The Society for Surgery of the Alimentary Tract, Inc.

Keywords
Gastric bypass, Gastritis, H. pylori, Morbid obesity, Pepsinogen
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-46647 (URN)10.1016/S1091-255X(03)00063-5 (DOI)
Available from: 2009-10-11 Created: 2009-10-11 Last updated: 2017-12-13
Mårdh, E., Mårdh, S., Mårdh, B. & Borch, K. (2002). Diagnosis of gastritis by means of a combination of serological analyses. Clinica Chimica Acta, 320(1-2), 17-27
Open this publication in new window or tab >>Diagnosis of gastritis by means of a combination of serological analyses
2002 (English)In: Clinica Chimica Acta, ISSN 0009-8981, E-ISSN 1873-3492, Vol. 320, no 1-2, p. 17-27Article in journal (Refereed) Published
Abstract [en]

Background: Gastroscopy and examination of biopsy is normally required for diagnosis of gastritis. This is costly and inconvenient for the patient, and there is a need for a simple pregastroscopic screening method to reduce the endoscopy workload. Our aim was to develop a serological screening test for gastritis. Methods: Sera from subjects examined with gastroscopy and biopsy were analyzed for H,K-ATPase antibodies, Helicobacter pylori antibodies and pepsinogen I. The diagnoses were normal gastric mucosa (n=50), duodenal ulcer (n=53) and atrophic corpus gastritis, with (n=50) or without pernicious anemia (n=46). Results: An evaluation scheme was constructed to optimize the diagnostic agreement between serology and gastric mucosal morphology. The sensitivity to detect gastritis was 98% (146/149) (95% CI 94-100%) and the specificity 84% (42/50) (95% CI 71-93%). Additional sera from 483 subjects from the general population were analyzed. There was a good agreement between serology and gastric mucosal morphology. Conclusions: Assays of multiple serum analytes are useful for the initial screening of gastritis. They are complementary to upper gastroscopy by identification of subjects with a normal gastric mucosa, those who qualify for eradication of H. pylori, and those who have developed atrophy and are at risk of developing malignancy and, therefore, require gastroscopic examination.

National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-24969 (URN)10.1016/S0009-8981(02)00040-2 (DOI)9380 (Local ID)9380 (Archive number)9380 (OAI)
Available from: 2009-10-07 Created: 2009-10-07 Last updated: 2017-12-13
Tømmerås, K., Hammer, P., Sundler, F., Borch, K., Mårdh, S. & Cabero, J. L. (2002). Immunolocalization of Cholecystokinin-2 Receptors in Rat Gastric Mucosa. Scandinavian Journal of Gastroenterology, 37(9), 1017-1024
Open this publication in new window or tab >>Immunolocalization of Cholecystokinin-2 Receptors in Rat Gastric Mucosa
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2002 (English)In: Scandinavian Journal of Gastroenterology, ISSN 0036-5521, E-ISSN 1502-7708, Vol. 37, no 9, p. 1017-1024Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: Gastrin exerts trophic effects on the gastric mucosa by mechanisms not yet completely elucidated. Our aim was to localize the cholecystokinin-2 (CCK2) receptor in epithelial cells of foetal and adult rat stomachs in order to determine the cell types that are directly affected by gastrin.

METHODS: Gastric tissue was subjected to indirect double immunofluorescence staining with antiserum against the C-terminal decapeptide of the CCK2 receptor and antibodies against 5' bromo-2-deoxyuridine, which had been injected into the rats I h before they were killed, the acid pump H,K-ATPase, the membrane-cytoskeletal linker ezrin, pepsin/pepsinogen or histidine decarboxylase.

RESULTS: Undifferentiated foetal gastric epithelial cells expressed CCK2 receptors, whereas stem cells of adult gastric glands did not exhibit immunoreactivity. However, other epithelial cells in the progenitor zone of adult gastric glands did express CCK2 receptors. Some of these cells were faintly stained for H,K-ATPase; pepsin/pepsinogen was also detected in this region. Parietal cells in the isthmus/pit region of the glands contained ezrin, and some showed weak immunoreactivity for the CCK2 receptor. As expected, enterochromaffin-like cells also expressed CCK2 receptors.

CONCLUSION: Our findings are consistent with the hypothesis that a CCK2 receptor mediates direct effects of gastrin on gastric epithelial cells during both stomach organogenesis and adult life.

Keywords
Cck2, Receptor, Gastrin, Immunofluorescence, Organogenesis, Progenitor Zone, Stomach
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-24956 (URN)10.1080/003655202320378194 (DOI)9367 (Local ID)9367 (Archive number)9367 (OAI)
Available from: 2009-10-07 Created: 2009-10-07 Last updated: 2017-12-13Bibliographically approved
Azerkan, L., Bengtsson, P., Tømmerås, K., Li, Z.-Q. & Mårdh, S. (2001). Characterization of oxyntic glands isolated from the rat gastric mucosa. Comparative Biochemistry and Physiology A, 128(2), 349-357
Open this publication in new window or tab >>Characterization of oxyntic glands isolated from the rat gastric mucosa
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2001 (English)In: Comparative Biochemistry and Physiology A, ISSN 1095-6433, E-ISSN 1531-4332, Vol. 128, no 2, p. 349-357Article in journal (Refereed) Published
Abstract [en]

A simple and reproducible method for isolating oxyntic glands from the rat gastric mucosa was developed. The mucosa was incubated with pronase and EGTA, and then treated mechanically to release glands that were separated from single cells by sedimentation. Parietal cells were identified by immunostaining using a monoclonal antibody against H,K-ATPase. The glandular cells appeared morphologically intact. By careful control of the conditions of gland isolation, long glandular structures comprising hundreds of cells surrounding the lumen were obtained. Intraperitoneal injection of Br-deoxyuridine in the rat 1.5 h before the isolation procedure resulted in glands with a labeling of cells in their neck region. The glands were viable, as demonstrated by their ability to respond to various hormones. Histamine dose-dependently stimulated the acid formation which was measured as the accumulation of [14C]aminopyrine. At 100 microM histamine the accumulation was increased 5-10-fold. At 100 nM, pentagastrin potentiated the histamine stimulated accumulation by approximately 40% but pentagastrin alone did not stimulate. The oxyntic glands obtained by the present procedure appear useful for studies on cell physiology, including regulation of acid secretion, cellular interactions, and possibly also differentiation and proliferation mechanisms since long glandular fragments that contained the proliferative zone could be isolated.

National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-24920 (URN)10.1016/S1095-6433(00)00309-3 (DOI)11223396 (PubMedID)9324 (Local ID)9324 (Archive number)9324 (OAI)
Available from: 2009-10-07 Created: 2009-10-07 Last updated: 2017-12-13Bibliographically approved
Taniguchi, K., Kaya, S., Abe, K. & Mårdh, S. (2001). The oligomeric nature of Na/K-transport ATPase. Journal of Biochemistry (Tokyo), 129(3), 335-342
Open this publication in new window or tab >>The oligomeric nature of Na/K-transport ATPase
2001 (English)In: Journal of Biochemistry (Tokyo), ISSN 0021-924X, E-ISSN 1756-2651, Vol. 129, no 3, p. 335-342Article in journal (Refereed) Published
Abstract [en]

Since the discovery of Na/K-ATPase, evidence has accumulated to suggest that 1 mol of ATP hydrolysis occurs via the Na+-occluded ADP-sensitive phosphoenzyme, the K+-sensitive phosphoenzyme and the K+-occluded enzyme accompanying active transport of 3Na+ and 2K+ according the Post-Albers scheme. However, some controversial issues have arisen concerning whether the functional unit of the enzyme is an a▀-protomer or a much higher oligomer, which would be related to the mechanism of transport, either sequential or simultaneous. Detailed studies of oligomer interaction and the reactivity of the enzyme and a comparison of the extent of phosphorylation with ligand-binding capacities in the presence or absence of ATP hydrolysis and others strongly suggest that the functional unit of the enzyme in the membrane is a tetraprotomer, (a▀)4. They also suggest that each reaction intermediate of the Post-Albers scheme, respectively, reflects half of the site property of the intermediate and that another half binds ATP. These data may be useful not only to answer the long-standing question of whether the mechanism functions in the presence of both Na+ and K+ but also contribute to a better understanding of the mechanism of P-type pump ATPase in general.

National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-25280 (URN)9720 (Local ID)9720 (Archive number)9720 (OAI)
Available from: 2009-10-07 Created: 2009-10-07 Last updated: 2017-12-13
Bengtsson, P., Azerkan, L., Lundqvist, G., Nilsson, G. & Mårdh, S. (2000). Effects of cholecystokinin on acid formation in glands and cells isolated from rabbit and rat gastric mucosa. Comparative Biochemistry and Physiology A, 126(1), 77-84
Open this publication in new window or tab >>Effects of cholecystokinin on acid formation in glands and cells isolated from rabbit and rat gastric mucosa
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2000 (English)In: Comparative Biochemistry and Physiology A, ISSN 1095-6433, E-ISSN 1531-4332, Vol. 126, no 1, p. 77-84Article in journal (Refereed) Published
Abstract [en]

Isolated gastric glands and isolated cells prepared from rabbit and rat were studied to analyse the influence of cholecystokinin octapeptide (CCK 8) on histamine stimulated parietal cell acid formation as assessed by [14C]aminopyrine sequestered in acid tissue compartments. In rabbit gastric glands, CCK 8 evoked 32±6% (P<0.01) inhibition of histamine stimulated acid formation, whereas in glands prepared from rat no inhibition was recorded. Instead, CCK 8 seemed to induce a variable increase of the histamine stimulation in rat gastric glands as the aminopyrine accumulation was increased by 110±46% (P<0.1). Further studies on cell preparations derived from rabbit gastric mucosa revealed dual properties of CCK 8, eliciting either inhibition or stimulation of the parietal cell depending on the presence of endocrine cells. The results show that paracrine communication may be effective in glandular preparations, but seems to vary depending on species.

Keywords
Inhibition of acid, Acid secretion, Fundic glands, In vitro, Paracrine function, Parietal cells, Histamine stimulation, Somatostatin
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-24925 (URN)10.1016/S1095-6433(00)00188-4 (DOI)10908854 (PubMedID)9330 (Local ID)9330 (Archive number)9330 (OAI)
Available from: 2009-10-07 Created: 2009-10-07 Last updated: 2017-12-13Bibliographically approved
Tømmerås, K., Cabero, J. L. & Mårdh, S. (2000). Expression of extracellular matrix proteins in the fetal rat gastric mucosa. Anatomy and Embryology, 201(3), 149-156
Open this publication in new window or tab >>Expression of extracellular matrix proteins in the fetal rat gastric mucosa
2000 (English)In: Anatomy and Embryology, ISSN 0340-2061, E-ISSN 1432-0568, Vol. 201, no 3, p. 149-156Article in journal (Refereed) Published
Abstract [en]

At gestational day 16 the epithelium of the rat stomach consists of a stratified layer of undifferentiated cells, and two days later glandular structures appear. The present study was carried out to identify extracellular matrix proteins that could be involved in the epithelial cell proliferation and differentiation processes that occur in the fetal rat stomach during this period. For comparative purposes the expression of the same components in the adult gastric mucosa was examined. Pregnant Sprague-Dawley rats received an intraperitoneal injection of 5-bromo-2’-deoxyuridine to label proliferating cells. One, 3.5, or 6 h post-injection the stomachs were excised and immediately frozen. The specimens were sectioned and stained with hematoxylin and eosin or for 5-bromo-2’-deoxyuridine, cytokeratin no. 8, H,K-ATPase, and the extracellular matrix proteins fibronectin, laminin, and collagens type I and IV. A stratified layer of proliferating cells was observed in the epithelium of the fetal stomachs, while in adult stomachs proliferating cells were detected in the isthmus/neck region of the glands. Cytokeratin, an epithelial cell marker, was sparse at gestational day 16 but abundant both at gestational day 18 and in the isthmus/neck region of gastric glands of the adult stomach. The parietal cell marker H,K-ATPase could not be detected in the fetal stomachs during this period. Fibronectin was observed in the stroma of both fetal and adult stomachs. Collagen type I could only be detected in the stroma close to the oesophagus at gestational day 16. Two days later, collagen type I was abundant in the lamina propria, the submucosa and in the serosa of the fetal stomachs. In adult tissue collagen type I was detected in the surface epithelium, the submucosa and in the serosa of the stomach. Collagen type IV and laminin were expressed in the lamina propria, the basement membranes around blood vessels, muscle cells, and nerve bundles, as well as in the serosa of both 16- and 18-day-old fetal and adult rat stomachs. In conclusion, a high cell proliferation rate was observed in the epithelium at both gestational days 16 and 18. The increased expression of cytokeratin observed during this period indicates that the epithelial character of the embryonic cells becomes more distinct, while the remarkable change in the expression of collagen type I might reflect an important role of collagen type I in the development of the gastric epithelium.

Keywords
Cell differentiation, Cell proliferation, Collagen, Fetal development, Fibronectin, Immunohistochemistry, Keratin, Laminin
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-25282 (URN)10.1007/PL00008236 (DOI)9722 (Local ID)9722 (Archive number)9722 (OAI)
Available from: 2009-10-07 Created: 2009-10-07 Last updated: 2017-12-13Bibliographically approved
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