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Hildesjö, Camilla
Alternative names
Publications (10 of 12) Show all publications
Sardar Sinha, M., Ansell - Schultz, A., Civitelli, L., Hildesjö, C., Larsson, M., Lannfelt, L., . . . Hallbeck, M. (2018). Alzheimers disease pathology propagation by exosomes containing toxic amyloid-beta oligomers. Acta Neuropathologica, 136(1), 41-56
Open this publication in new window or tab >>Alzheimers disease pathology propagation by exosomes containing toxic amyloid-beta oligomers
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2018 (English)In: Acta Neuropathologica, ISSN 0001-6322, E-ISSN 1432-0533, Vol. 136, no 1, p. 41-56Article in journal (Refereed) Published
Abstract [en]

The gradual deterioration of cognitive functions in Alzheimers disease is paralleled by a hierarchical progression of amyloid-beta and tau brain pathology. Recent findings indicate that toxic oligomers of amyloid-beta may cause propagation of pathology in a prion-like manner, although the underlying mechanisms are incompletely understood. Here we show that small extracellular vesicles, exosomes, from Alzheimer patients brains contain increased levels of amyloid-beta oligomers and can act as vehicles for the neuron-to-neuron transfer of such toxic species in recipient neurons in culture. Moreover, blocking the formation, secretion or uptake of exosomes was found to reduce both the spread of oligomers and the related toxicity. Taken together, our results imply that exosomes are centrally involved in Alzheimers disease and that they could serve as targets for development of new diagnostic and therapeutic principles.

Place, publisher, year, edition, pages
SPRINGER, 2018
Keywords
Alzheimers disease; Exosomes; Oligomers; Beta-amyloid; Human; Prion-like; Propagation
National Category
Neurosciences
Identifiers
urn:nbn:se:liu:diva-149701 (URN)10.1007/s00401-018-1868-1 (DOI)000435940000003 ()29934873 (PubMedID)
Note

Funding Agencies|Swedish Research Council [MH: 523-2013-2735]; Swedish Alzheimer foundation; Swedish Brain Foundation; Hans-Gabriel and Alice Trolle-Wachtmeister Foundation for Medical Research; Konung Gustaf V:s och Drottning Victorias Frimurarestiftelse; Marianne and Marcus Wallenberg Foundation; Swedish Fund for Research without Animal Experiments; Swedish Dementia Foundation; Linkoping University Neurobiology Centre; County Council of Ostergotland

Available from: 2018-07-18 Created: 2018-07-18 Last updated: 2019-10-14
Blockhuys, S., Celauro, E., Hildesjö, C., Feizi, A., Stål, O., Fierro-González, J. & Wittung-Stafshede, P. (2017). Defining the human copper proteome and analysis of its expression variation in cancers.. Metallomics : integrated biometal science, 9(2), 112-123
Open this publication in new window or tab >>Defining the human copper proteome and analysis of its expression variation in cancers.
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2017 (English)In: Metallomics : integrated biometal science, ISSN 1756-591X, Vol. 9, no 2, p. 112-123Article in journal (Refereed) Published
Abstract [en]

Copper (Cu) is essential for living organisms, and acts as a cofactor in many metabolic enzymes. To avoid the toxicity of free Cu, organisms have specific transport systems that 'chaperone' the metal to targets. Cancer progression is associated with increased cellular Cu concentrations, whereby proliferative immortality, angiogenesis and metastasis are cancer hallmarks with defined requirements for Cu. The aim of this study is to gather all known Cu-binding proteins and reveal their putative involvement in cancers using the available database resources of RNA transcript levels. Using the database along with manual curation, we identified a total of 54 Cu-binding proteins (named the human Cu proteome). Next, we retrieved RNA expression levels in cancer versus normal tissues from the TCGA database for the human Cu proteome in 18 cancer types, and noted an intricate pattern of up- and downregulation of the genes in different cancers. Hierarchical clustering in combination with bioinformatics and functional genomics analyses allowed for the prediction of cancer-related Cu-binding proteins; these were specifically inspected for the breast cancer data. Finally, for the Cu chaperone ATOX1, which is the only Cu-binding protein proposed to have transcription factor activities, we validated its predicted over-expression in patient breast cancer tissue at the protein level. This collection of Cu-binding proteins, with RNA expression patterns in different cancers, will serve as an excellent resource for mechanistic-molecular studies of Cu-dependent processes in cancer.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2017
National Category
Cancer and Oncology
Identifiers
urn:nbn:se:liu:diva-135375 (URN)10.1039/c6mt00202a (DOI)000397437300002 ()27942658 (PubMedID)
Note

Funding agencies: Swedish Society for Medical Research; Folke and Marianne Edler Research Fund; Lars Hierta Memorial Foundation; Knut and Alice Wallenberg Foundation; Swedish Research Council; Chalmers Foundation; NBIS (National Bioinformatics Infrastructure Sweden)

Available from: 2017-03-14 Created: 2017-03-14 Last updated: 2018-05-03Bibliographically approved
Blockhuys, S., Rani Agarwal, N., Hildesjö, C., Jarlsfelt, I., Wittung-Stafshede, P. & Sun, X.-F. (2017). Second harmonic generation for collagen I characterization in rectal cancer patients with and without preoperative radiotherapy. Journal of Biomedical Optics, 22(10), Article ID 106006.
Open this publication in new window or tab >>Second harmonic generation for collagen I characterization in rectal cancer patients with and without preoperative radiotherapy
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2017 (English)In: Journal of Biomedical Optics, ISSN 1083-3668, E-ISSN 1560-2281, Vol. 22, no 10, article id 106006Article in journal (Refereed) Published
Abstract [en]

Rectal cancer is treated with preoperative radiotherapy (RT) to downstage the tumor, reduce local recurrence, and improve patient survival. Still, the treatment outcome varies significantly and new biomarkers are desired. Collagen I (Col-I) is a potential biomarker, which can be visualized label-free by second harmonic generation (SHG). Here, we used SHG to identify Col-I changes induced by RT in surgical tissue, with the aim to evaluate the clinical significance of RT-induced Col-I changes. First, we established a procedure for quantitative evaluation of Col-I by SHG in CDX2-stained tissue sections. Next, we evaluated Col-I properties in material from 31 non-RT and 29 RT rectal cancer patients. We discovered that the Col-I intensity and anisotropy were higher in the tumor invasive margin than in the inner tumor and normal mucosa, and RT increased and decreased the intensity in inner tumor and normal mucosa, respectively. Furthermore, higher Col-I intensity in the inner tumor was related to increased distant recurrence in the non-RT group but to longer survival in the RT group. In conclusion, we present a new application of SHG for quantitative analysis of Col-I in surgical material, and the first data suggest Col-I intensity as a putative prognostic biomarker in rectal cancer. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License.

Place, publisher, year, edition, pages
SPIE - International Society for Optical Engineering, 2017
Keywords
collagen I; second harmonic generation; prognosis; rectal cancer; radiotherapy
National Category
Radiology, Nuclear Medicine and Medical Imaging
Identifiers
urn:nbn:se:liu:diva-143095 (URN)10.1117/1.JBO.22.10.106006 (DOI)000414251000019 ()29019178 (PubMedID)2-s2.0-85032872703 (Scopus ID)
Note

Funding Agencies|Swedish Cancer Foundation; Research Council of South East Sweden; Liu Cancer; Trygger Foundation; Swedish Research Council; Knut och Alice Wallenberg Foundation

Available from: 2017-11-20 Created: 2017-11-20 Last updated: 2018-05-03Bibliographically approved
Haj-Hosseini, N., Milos, P., Hildesjö, C., Hallbeck, M., Richter, J. & Wårdell, K. (2016). Fluorescence spectroscopy and optical coherence tomography for brain tumor detection. In: : . Paper presented at SPIE Photonics Europe, Biophotonics: Photonic Solutions for Better Health Care, Brussels, Belgium, 3 - 7 April 2016 (pp. 9887-96). SPIE - International Society for Optical Engineering
Open this publication in new window or tab >>Fluorescence spectroscopy and optical coherence tomography for brain tumor detection
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2016 (English)Conference paper, Oral presentation with published abstract (Refereed)
Abstract [en]

Resection of brain tumor is a challenging task as the tumor does not have clear borders and the malignant types specifically have often a diffuse and infiltrative pattern of growth. Recently, neurosurgical microscopes have been modified to incorporate fluorescence modules for detection of tumor when 5-aminolevulinic acid (5-ALA) is used as a contrast. We have in combination with the fluorescence microscopes implemented and evaluated a fluorescence spectroscopy based handheld probe for detecting the 5-aminolevulinic acid (ALA) induced protoporphyrin IX (PpIX) in the gliomas in 50 patients intraoperatively. The results show a significantly high sensitivity for differentiating tumor from the healthy tissue and distinguished fluorescence intensity levels in the tumor cell infiltration zone around the tumor. However, knowledge on association of the quantified fluorescence signals specifically in the intermediate inflammatory zone with the infiltrative tumor cells can be complemented with volumetric tissue imaging and a higher precision histopathological analysis. In this work, a spectral domain optical coherence tomography (OCT) system with central wavelength of 1325nm has been used to image the tissue volume that the fluorescence is collected from and is evaluated against histopathological analysis for a higher precision slicing. The results show that although healthy brain has a homogenous microstructure in the OCT images, the brain tumor shows a distinguished texture in the images correlated with the PpIX fluorescence intensity and histopathology.

Place, publisher, year, edition, pages
SPIE - International Society for Optical Engineering, 2016
Keywords
Brain tumor, fluorescence, optical coherence tomography, hjärntumör, fluorescens, optisk koherenstomografi
National Category
Other Medical Engineering
Identifiers
urn:nbn:se:liu:diva-124008 (URN)
Conference
SPIE Photonics Europe, Biophotonics: Photonic Solutions for Better Health Care, Brussels, Belgium, 3 - 7 April 2016
Funder
Medical Research Council of Southeast Sweden (FORSS)
Available from: 2016-01-18 Created: 2016-01-18 Last updated: 2019-10-14Bibliographically approved
Haj-Hosseini, N., Milos, P., Richter, J., Hildesjö, C., Hallbeck, M. & Wårdell, K. (2015). Detection of brain tumor using fluorescence and optical coherence tomography. In: : . Paper presented at Medicinteknikdagarna 2015, 13–14 oktober 2015 Uppsala Konsert & Kongress. Uppsala
Open this publication in new window or tab >>Detection of brain tumor using fluorescence and optical coherence tomography
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2015 (English)Conference paper, Oral presentation with published abstract (Refereed)
Abstract [en]

Resection of brain tumor is a challenging task as the tumor does not have clear borders and the malignant types specifically have often a diffuse and infiltrative pattern of growth. We have previously implemented and evaluated a fluorescence spectroscopy based handheld probe for detecting the 5-aminolevulinic acid induced protoporphyrin IX (PpIX) in the gliomas. To add another dimension to the brain tumor detection and volumetric analysis of the tissue that exhibits fluorescence, optical coherence tomography was investigated on tumor specimens.

Material and Methods:

A fluorescence microscopy and a spectroscopy system as reported previously were used for detecting the fluorescence signals [1, 2]. A total of 50 patients have been included for intraoperative assessment of the tumor borders using the fluorescence techniques. A spectral domain OCT imaging system (TELESTO II, Thorlabs, Inc., NJ, USA) with central wavelength of 1325 nm was used to study the tissue microstructure post operatively. The system has a resolution of 13 and 5.5 μm in the lateral and axial directions, respectively. Tissue specimens from three patients undergoing brain tumor surgery were studied using the OCT system.

Results and Conclusion:

Using fluorescence spectroscopy the tumor could be detected with a sensitivity of 0.84 which was significantly higher than that of the surgical microscope (0.30). Brain tissue appeared rather homogeneous in the OCT images however the highly malignant tissue showed a clear structural difference from the non-malignant or low malignant brain tumor tissue which could be related to the fluorescence signal intensities.

Place, publisher, year, edition, pages
Uppsala: , 2015
Keywords
fluorescence, optical coherence tomography, brain tumor
National Category
Other Medical Engineering
Identifiers
urn:nbn:se:liu:diva-122286 (URN)
Conference
Medicinteknikdagarna 2015, 13–14 oktober 2015 Uppsala Konsert & Kongress
Funder
Swedish Research CouncilSwedish Childhood Cancer Foundation
Available from: 2015-10-27 Created: 2015-10-27 Last updated: 2019-10-14Bibliographically approved
Wickham, A., Sjölander, D., Bergström, G., Wang, E., Rajendran, V., Hildesjö, C., . . . Aili, D. (2015). Near-Infrared Emitting and Pro-Angiogenic Electrospun Conjugated Polymer Scaffold for Optical Biomaterial Tracking. Advanced Functional Materials, 25(27), 4274-4281
Open this publication in new window or tab >>Near-Infrared Emitting and Pro-Angiogenic Electrospun Conjugated Polymer Scaffold for Optical Biomaterial Tracking
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2015 (English)In: Advanced Functional Materials, ISSN 1616-301X, E-ISSN 1616-3028, Vol. 25, no 27, p. 4274-4281Article in journal (Refereed) Published
Abstract [en]

Noninvasive tracking of biomaterials is vital for determining the fate and degradation of an implant in vivo, and to show its role in tissue regeneration. Current biomaterials have no inherent capacity to enable tracing but require labeling with, for example, fluorescent dyes, or nanoparticles. Here a novel biocompatible fully conjugated electrospun scaffold is described, based on a semiconducting luminescent polymer that can be visualized in situ after implantation using fluorescence imaging. The polymer, poly [2,3-bis-(3-octyloxyphenyl)quinoxaline-5,8-diyl-alt -thiophene-2,5-diyl] (TQ1), is electrospun to form a fibrous mat. The fibers display fluorescence emission in the near-infrared region with lifetimes in the sub-nanosecond range, optimal for in situ imaging. The material shows no cytotoxic behaviors for embryonic chicken cardiomyocytes and mouse myoblasts, and cells migrate onto the TQ1 fibers even in the presence of a collagen substrate. Subcutaneous implantations of the material in rats show incorporation of the TQ1 fibers within the tissue, with limited inflammation and a preponderance of small capillaries around the fibers. The fluorescent properties of the TQ1 fibers are fully retained for up to 90 d following implantation and they can be clearly visualized in tissue using fluorescence and lifetime imaging, thus making it both a pro-angiogenic and traceable biomaterial.

Place, publisher, year, edition, pages
Wiley: 12 months, 2015
Keywords
biomaterials, conjugated polymers, near-infrared, angiogenesis, electrospinning
National Category
Biomaterials Science Polymer Chemistry
Identifiers
urn:nbn:se:liu:diva-120449 (URN)10.1002/adfm.201500351 (DOI)000357996600011 ()
Note

Funding Agencies|Linkoping University; Swedish Foundation for Strategic Research; Swedish Research Council

Available from: 2015-08-12 Created: 2015-08-11 Last updated: 2017-12-04
Danielsson, P., Fredriksson, C. & Huss, F. (2009). A Novel Concept for Treating Large Necrotizing Fasciitis Wounds With Bilayer Dermal Matrix, Split-thickness Skin Grafts, and Negative Pressure Wound Therapy. Wounds (King of Prussia, Pa.), 21(8), 215-220
Open this publication in new window or tab >>A Novel Concept for Treating Large Necrotizing Fasciitis Wounds With Bilayer Dermal Matrix, Split-thickness Skin Grafts, and Negative Pressure Wound Therapy
2009 (English)In: Wounds (King of Prussia, Pa.), ISSN 1044-7946, E-ISSN 1943-2704, Vol. 21, no 8, p. 215-220Article in journal (Refereed) Published
Abstract [en]

Treatment of necrotizing fasciitis (NF) includes radical surgical debridement often resulting in large wounds that need to be closed with methods including split-thickness skin grafts (STSG), local flaps, or guided tissue regeneration procedures. In this case report, a 45 year-old Caucasian male was surgically treated for a benign left groin hernia, developed NF, and was transferred to the authors burn unit. The wound was treated initially with wide debridement and with a brief delay before finally closing the wound. A collagen matrix such as Integra (R) Dermal Regeneration Template (Integra LifeSciences, Plainsboro, NJ) in combination with STSG and negative pressure wound treatment, can provide fast recovery resulting in pliable, functional skin.

National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-20581 (URN)000269472900006 ()
Note

Funding text: "We express our sincere gratitude to Mrs. Kristina Briheim and Mrs. Anita Lonn, Senior Laboratory Technicians at the Laboratory for Experimental Plastic Surgery, Institute of Biomedicine and Surgery, Faculty Of Health Sciences, Linkoping Universitet, Linkoping, Sweden."

Available from: 2009-09-15 Created: 2009-09-15 Last updated: 2017-12-13Bibliographically approved
Fredriksson, C., Kratz, G. & Huss, F. (2009). Accumulation of Silver and Delayed Re-epithelialization in Normal Human Skin: An ex-vivo Study of Different Silver Dressings. WOUNDS-A COMPENDIUM OF CLINICAL RESEARCH AND PRACTICE, 21(5), 116-123
Open this publication in new window or tab >>Accumulation of Silver and Delayed Re-epithelialization in Normal Human Skin: An ex-vivo Study of Different Silver Dressings
2009 (English)In: WOUNDS-A COMPENDIUM OF CLINICAL RESEARCH AND PRACTICE, ISSN 1044-7946, Vol. 21, no 5, p. 116-123Article in journal (Refereed) Published
Abstract [en]

Silver is commonly used in wound dressings and topical formulations to assist in the management of wounds that are infected or at risk of becoming infected. They provide potent broad-spectrum antimicrobial activity, but should not cause sustained staining of the skin, dermal or systemic accumulation of silver, or discomfort to the patient. However, clinicians and healthcare personnel have been concerned about topical staining of the skin and complaints of additional pain from patients treated with certain silver dressings. Some delay in re-epithelialization has also been noticed and reported. The reasons for this are not clear, and the authors believed further study regarding the possible effects of silver accumulation and silver dressings effect on re-epithelialization was required. The authors studied possible silver accumulation and re-epithelialization in normal human dermal skin. The results showed that most of the dressings or treatments discolored the wound surface and that there was a dermal accumulation of what were assumed to be silver particles. Varying grades of accumulation were found in deep dermal tissue, particularly around blood vessels, depending on the dressing used. The results also indicated that all of the tested products delayed re-epithelialization in this model.

National Category
Cell and Molecular Biology
Identifiers
urn:nbn:se:liu:diva-19124 (URN)
Available from: 2009-06-12 Created: 2009-06-12 Last updated: 2018-01-13Bibliographically approved
Fredriksson, C., Ilias, M. & Anderson, C. (2009). New mechanical device for effective removal of skin tags in routine health care. Dermatologi Online, 15(2), Article ID 9.
Open this publication in new window or tab >>New mechanical device for effective removal of skin tags in routine health care
2009 (English)In: Dermatologi Online, ISSN 1087-2108, E-ISSN 1087-2108, Vol. 15, no 2, article id 9Article in journal (Refereed) Published
Abstract [en]

Skin tags (acrochordons) are exceedingly common benign skin lesions. A novel medical device in the form of a flat adhesive patch applies pressure to the base of a skin tag, leading to its removal within 3-6 days. The device was used in a clinical trial to treat and remove skin tags of the neck, upper torso, and axillae in volunteers. In this study, a total of 177 skin tags were treated in 32 individuals. One hundred seventy-two lesions fulfilled intention to treat (ITT) criteria. A majority of ITT lesions (90%) reached final assessment. Successful outcome was highest (90%) for lesions up to 1 mm in base. For lesions up to 2 mm, the rate of successful outcome was 76 percent. The desired outcome was seen in 65 percent of all ITT lesions. The cosmetic outcome after removal was excellent. Discomfort was assessed as minimal during all stages of the procedure. Analysis of data on blood flow in the skin tags during the treatment showed that the outcome was influenced by whether a decrease in blood flow was achieved immediately after application and at 2-3 days, but that the degree of occlusion was not critical. The results of this study illustrate that the device presents a new option for the management of unmet needs in the treatment of skin tags.

Place, publisher, year, edition, pages
University of California, 2009
National Category
Dermatology and Venereal Diseases
Identifiers
urn:nbn:se:liu:diva-18884 (URN)19336026 (PubMedID)
Available from: 2009-06-05 Created: 2009-06-05 Last updated: 2017-12-13Bibliographically approved
Fredriksson, C., Hedhammar, M., Feinstein, R., Nordling, K., Kratz, G., Johansson, J., . . . Rising, A. (2009). Tissue Response to Subcutaneously Implanted Recombinant Spider Silk: An in Vivo Study. Materials, 2(4), 1908-1922
Open this publication in new window or tab >>Tissue Response to Subcutaneously Implanted Recombinant Spider Silk: An in Vivo Study
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2009 (English)In: Materials, ISSN 1996-1944, E-ISSN 1996-1944, Vol. 2, no 4, p. 1908-1922Article in journal (Refereed) Published
Abstract [en]

Spider silk is an interesting biomaterial for medical applications. Recently, a method for production of recombinant spider silk protein (4RepCT) that forms macroscopic fibres in physiological solution was developed. Herein, 4RepCT and Mersilk(TM) (control) fibres were implanted subcutaneously in rats for seven days, without any negative systemic or local reactions. The tissue response, characterised by infiltration of macrophages and multinucleated cells, was similar with both fibres, while only the 4RepCT-fibres supported ingrowth of fibroblasts and newly formed capillaries. This in vivo study indicates that 4RepCT-fibres are well tolerated and could be used for medical applications, e. g., tissue engineering.

Place, publisher, year, edition, pages
Basel, Switzerland: MDPI AG, 2009
Keywords
spider silk, recombinant, biocompatibility, in vivo
National Category
Materials Engineering
Identifiers
urn:nbn:se:liu:diva-103169 (URN)10.3390/ma2041908 (DOI)000208140200019 ()
Available from: 2014-01-14 Created: 2014-01-14 Last updated: 2017-12-06Bibliographically approved
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