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Forsum, Urban
Publications (10 of 78) Show all publications
Larsson, P.-G., Brandsborg, E., Forsum, U., Pendharkar, S., Krogh Andersen, K., Nasic, S., . . . Marcotte, H. (2011). Extended antimicrobial treatment of bacterial vaginosis combined with human lactobacilli to find the best treatment and minimize the risk of relapses. BMC Infectious Diseases, 11(223)
Open this publication in new window or tab >>Extended antimicrobial treatment of bacterial vaginosis combined with human lactobacilli to find the best treatment and minimize the risk of relapses
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2011 (English)In: BMC Infectious Diseases, ISSN 1471-2334, E-ISSN 1471-2334, Vol. 11, no 223Article in journal (Refereed) Published
Abstract [en]

Background: The primary objective of this study was to investigate if extended antibiotic treatment against bacterial vaginosis (BV) together with adjuvant lactobacilli treatment could cure BV and, furthermore, to investigate factors that could cause relapse. less thanbrgreater than less thanbrgreater thanMethods: In all, 63 consecutive women with bacterial vaginosis diagnosed by Amsel criteria were offered a much more aggressive treatment of BV than used in normal clinical practice with repeated antibiotic treatment with clindamycin and metronidazole together with vaginal gelatine capsules containing different strains of lactobacilli both newly characterised and a commercial one (10(9) freeze-dried bacteria per capsule). Oral clindamycin treatment was also given to the patients sexual partner. less thanbrgreater than less thanbrgreater thanResults: The cure rate was 74.6% after 6 months. The patients were then followed as long as possible or until a relapse. The cure rate was 65.1% at 12 months and 55.6% after 24 months. There was no significant difference in cure rate depending on which Lactobacillus strains were given to the women or if the women were colonised by lactobacilli. The most striking factor was a new sex partner during the follow up period where the Odds Ratio of having a relapse was 9.3 (2.8-31.2) if the patients had a new sex partner during the observation period. less thanbrgreater than less thanbrgreater thanConclusions: The study shows that aggressive treatment of the patient with antibiotics combined with specific Lactobacillus strain administration and partner treatment can provide long lasting cure. A striking result of our study is that change of partner is strongly associated with relapse of BV.

Place, publisher, year, edition, pages
BioMed Central, 2011
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-71557 (URN)10.1186/1471-2334-11-223 (DOI)000295288800001 ()
Note
Funding Agencies|Bifodan AS Denmark||Swedish research Council in Sweden||Bifodan AS||Available from: 2011-10-21 Created: 2011-10-21 Last updated: 2017-12-08
Sundén, B., Larsson, M., Falkeborn, T., Paues, J., Forsum, U., Lindh, M., . . . Serrander, L. (2011). Real-time PCR detection of Human Herpesvirus 1-5 in patients lacking clinical signs of a viral CNS infection. BMC Infectious Diseases, 11(220)
Open this publication in new window or tab >>Real-time PCR detection of Human Herpesvirus 1-5 in patients lacking clinical signs of a viral CNS infection
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2011 (English)In: BMC Infectious Diseases, ISSN 1471-2334, E-ISSN 1471-2334, Vol. 11, no 220Article in journal (Refereed) Published
Abstract [en]

BACKGROUND:

Infections of the central nervous system (CNS) with herpes- or enterovirus can be self-limiting and benign, but occasionally result in severe and fatal disease. The polymerase chain reaction (PCR) has revolutionized the diagnostics of viral pathogens, and by multiple displacement amplification (MDA) prior to real-time PCR the sensitivity might be further enhanced. The aim of this study was to investigate if herpes- or enterovirus can be detected in cerebrospinal fluid (CSF) from patients without symptoms.

METHODS:

Cerebrospinal fluid (CSF) samples from 373 patients lacking typical symptoms of viral CNS infection were analysed by real-time PCR targeting herpesviruses or enteroviruses with or without prior MDA.

RESULTS:

In total, virus was detected in 17 patients (4%). Epstein-Barr virus (EBV) was most commonly detected, in general from patients with other conditions (e.g. infections, cerebral hemorrhage). MDA satisfactorily amplified viral DNA in the absence of human nucleic acids, but showed poor amplification capacity for viral DNA in CSF samples, and did not increase the sensitivity for herpes virus-detection with our methodology.

CONCLUSIONS:

Viral pathogens are rarely detected in CSF from patients without signs of CNS infection, supporting the view that real-time PCR is a highly specific method to detect symptomatic CNS-infection caused by these viruses. However, EBV may be subclinically reactivated due to other pathological conditions in the CNS.

Place, publisher, year, edition, pages
BioMed Central, 2011
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-71558 (URN)10.1186/1471-2334-11-220 (DOI)000295288300001 ()21849074 (PubMedID)
Note

Funding Agencies|Linkoping University|ALF: LIO-17791 |

Available from: 2011-10-21 Created: 2011-10-21 Last updated: 2018-03-26
Eriksson, K., Larsson, P.-G., Nilsson, M. & Forsum, U. (2011). Vaginal retention of locally administered clindamycin. APMIS, 119(6), 373-376
Open this publication in new window or tab >>Vaginal retention of locally administered clindamycin
2011 (English)In: APMIS, ISSN 0903-4641, Vol. 119, no 6, p. 373-376Article in journal (Refereed) Published
Abstract [en]

Since bacterial vaginosis (BV) is characterized by a lack of, or very few, lactobacilli and high numbers of small, mostly anaerobic bacteria, an obvious treatment modality would be eradication of the BV-associated bacterial flora followed by reintroduction of lactobacilli vaginally. As probiotic treatment with lactobacilli is one tool for improving the cure rate when treating BV, it is necessary to know the length of time after treatment that clindamycin can be found in the vagina and if this could interfere with the growth of the probiotic lactobacilli. We evaluated the vaginal concentration of clindamycin in 12 women for 8 days to obtain data on the concentration of clindamycin in the vagina after intravaginal treatment with the drug. The participants were examined five times between two menstrual periods: before treatment, the day after treatment was finished, and 3, 5 and 8 days post-treatment. The first day post-treatment clindamycin 0.46 x 10-3 to 8.4 x 10-3 g/g vaginal fluid (median 2.87 x 10-3) was found. Thereafter, the concentration of clindamycin decreased rapidly. In 10 patients clindamycin was found after 3 days. A very low concentration was still present 5 days after treatment in four patients. After 8 days no clindamycin was found. Clindamycin is rapidly eliminated from the vagina, within 3-8 days, after local administration. Our results indicate that treatment with probiotic lactobacilli could be problematic if carried out within 5 days after cessation of clindamycin treatment.

Place, publisher, year, edition, pages
Blackwell Publishing Ltd, 2011
Keyword
Treatment, clindamycin, bacterial vaginosis, concentration
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-68694 (URN)10.1111/j.1600-0463.2011.02742.x (DOI)000290626500007 ()
Available from: 2011-05-27 Created: 2011-05-27 Last updated: 2012-03-25Bibliographically approved
Pontet, F., Nordin, G., Dybkaer, R., Forsum, U. & Fuentes-Arderiu, X. (2011). WHAT ARE NOMINAL PROPERTIES AND EXAMINATIONS? in CLINICAL CHEMISTRY AND LABORATORY MEDICINE, vol 49, issue , pp S411-S411. In: CLINICAL CHEMISTRY AND LABORATORY MEDICINE (pp. S411-S411). Walter de Gruyter, 49
Open this publication in new window or tab >>WHAT ARE NOMINAL PROPERTIES AND EXAMINATIONS? in CLINICAL CHEMISTRY AND LABORATORY MEDICINE, vol 49, issue , pp S411-S411
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2011 (English)In: CLINICAL CHEMISTRY AND LABORATORY MEDICINE, Walter de Gruyter , 2011, Vol. 49, p. S411-S411Conference paper, Published paper (Refereed)
Abstract [en]

n/a

Place, publisher, year, edition, pages
Walter de Gruyter, 2011
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-68693 (URN)000289974200653 ()
Available from: 2011-05-27 Created: 2011-05-27 Last updated: 2011-05-30
Nordin, G., Dybkaer, R., Forsum, U., Fuentes-Arderiu, X., Schadow, G. & Pontet, F. (2010). An outline for a vocabulary of nominal properties and examinations - basic and general concepts and associated terms. CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 48(11), 1553-1566
Open this publication in new window or tab >>An outline for a vocabulary of nominal properties and examinations - basic and general concepts and associated terms
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2010 (English)In: CLINICAL CHEMISTRY AND LABORATORY MEDICINE, ISSN 1434-6621, Vol. 48, no 11, p. 1553-1566Article in journal (Refereed) Published
Abstract [en]

Scientists of disciplines in clinical laboratory sciences have long recognized the need for a common language for efficient and safe request of investigations, reporting of results, and communication of experience and scientific achievements. Widening the scope, most scientific disciplines, not only clinical laboratory sciences, rely to some extent on various nominal examinations, in addition to measurements. The International vocabulary of metrology - Basic and general concepts and associated terms (VIM) is designed for metrology, science of measurement. The aim of the proposed vocabulary is to suggest definitions and explanations of concepts and terms related to nominal properties, i.e., properties that can be compared for identity with other properties of the same kind-of-property, but that have no magnitude.

Place, publisher, year, edition, pages
Walter de Gruyter, 2010
Keyword
concept, examination, international, nominal property, term, vocabulary
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-62749 (URN)10.1515/CCLM.2010.325 (DOI)000284101000004 ()
Available from: 2010-12-03 Created: 2010-12-03 Last updated: 2010-12-03
Eriksson, K., Adolfsson, A.-S., Forsum, U. & Larsson, P.-G. (2010). The prevalence of BV in the population on the Åland Islands during a 15-year period. APMIS, 118(11), 903-908
Open this publication in new window or tab >>The prevalence of BV in the population on the Åland Islands during a 15-year period
2010 (English)In: APMIS, ISSN 0903-4641, Vol. 118, no 11, p. 903-908Article in journal (Refereed) Published
Abstract [en]

The aim of the study was to describe the prevalence and age distribution of bacterial vaginosis (BV) during an observation period of 15 years in a population study with cross-sectional samples of adult women living on the Aland Islands. The Aland Islands form an archipelago in the Baltic Sea and are a province of Finland. Every fifth year, specific age groups in the adult female population are invited to participate in a screening program for early diagnosis of cervical cancer using a papanicolaou (PAP)-stained vaginal smear. Women in the age groups of 20, 25, 30, 35, 40, 45, 50, 55, and 60 years are called each year. BV diagnosis of the PAP-stained smears uses the classification according to Nugent. The PAP-stained smears from the screening program of cervical cancer 1993, 1998, 2003, and 2008 were used in this study. A total of 3456 slides were investigated and 271 women could be followed for the 15-year observation period. The prevalence of BV declined from 15.6% in 1993 to 8.6% in 2008. The highest prevalence occurred among the age groups of 35 and 50 years. Among the 271 women who could be followed for the 15-year observation period, two-third showed normal/intermediate flora and one-third were infected with BV at least once. As this is a cross-sectional population study spanning 15 years, the prevalence of BV in the female adult population of the Aland Islands can be estimated. The prevalence has declined between 1993 and 2008 from 15.6% to 8.6%.

Place, publisher, year, edition, pages
Blackwell Publishing Ltd, 2010
Keyword
BV, prevalence, population study, diagnostics, PAP smear
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-61210 (URN)10.1111/j.1600-0463.2010.02678.x (DOI)000283161100012 ()
Available from: 2010-11-05 Created: 2010-11-05 Last updated: 2011-06-08Bibliographically approved
Forsum, U. (2009). Letter: En intressant fotnot [Letter to the editor]. Läkartidningen, 106(44), 2866
Open this publication in new window or tab >>Letter: En intressant fotnot
2009 (English)In: Läkartidningen, ISSN 0023-7205, E-ISSN 1652-7518, Vol. 106, no 44, p. 2866-Article in journal, Letter (Other academic) Published
Abstract [en]

[No abstract available]

National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-52986 (URN)
Available from: 2010-01-14 Created: 2010-01-14 Last updated: 2017-12-12Bibliographically approved
Jakobsson, T. & Forsum, U. (2008). Changes in the predominant human Lactobacillus flora during in vitro fertilisation. Annals of Clinical Microbiology and Antimicrobials, 7, Article ID 14.
Open this publication in new window or tab >>Changes in the predominant human Lactobacillus flora during in vitro fertilisation
2008 (English)In: Annals of Clinical Microbiology and Antimicrobials, ISSN 1476-0711, E-ISSN 1476-0711, Vol. 7, article id 14Article in journal (Refereed) Published
Abstract [en]

Background: Signature matching of nucleotide sequences in the V1 and V3 regions 16S rRNA genes using pyrosequencing technology is a powerful tool for typing vaginal Lactobacilli to the species level and has been used for investigating the vaginal microbial niche. Methods: This study has characterized the normal cultivable vaginal Lactobacillus flora at varying estradiol levels in plasma, the study comprised 17 patients undergoing ovarian stimulation for In Vitro Fertilization (IVF) treatment. The vaginal status of each participant was initially assessed as normal according to Amsel and Nugent criteria. Results: L. crispatus, L. gasseri and/or L. jensenii were present in 10of the patients throughout the study period, and little variation among these three species was encountered in individual patients. The flora of three women was dominated by L. delbrüeckii, L. rhamnosus or L. vaginalis. One woman exhibited a dominance of L. iners. The flora of the remaining three women were initially dominated by L. rhamnosus or L. reuteri, but as their estrogen levels rose, their flora composition altered, to become dominated by one of the three species most common in a normal, healthy vagina. Conclusion: Signature matching of nucleotide sequences in the V1 and V3 regions of 16S rRNA genes is a discriminative tool for the study of vaginal Lactobacilli and can be used to track the Lactobacillus flora under a variety of physiological conditions. © 2008 Jakobsson and Forsum, licensee BioMed Central Ltd.

Place, publisher, year, edition, pages
BioMed Central, 2008
Keyword
Bacterial Proteins/genetics Bacterial Typing Techniques Base Sequence DNA, Bacterial/genetics DNA, Ribosomal/genetics Female *Fertilization in Vitro Humans Lactobacillus/genetics/*isolation & purification Molecular Sequence Data Polymerase Chain Reaction
National Category
Other Biological Topics
Identifiers
urn:nbn:se:liu:diva-43432 (URN)10.1186/1476-0711-7-14 (DOI)18590533 (PubMedID)73836 (Local ID)73836 (Archive number)73836 (OAI)
Available from: 2009-10-10 Created: 2009-10-10 Last updated: 2017-12-13Bibliographically approved
Palmqvist, E., Aspevall, O., Burman, E., Nordin, G., Svahn, A. & Forsum, U. (2008). Difficulties for primary health care staff in interpreting bacterial findings on a device for simplified urinary culture. Scandinavian Journal of Clinical and Laboratory Investigation, 68(4), 312-316
Open this publication in new window or tab >>Difficulties for primary health care staff in interpreting bacterial findings on a device for simplified urinary culture
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2008 (English)In: Scandinavian Journal of Clinical and Laboratory Investigation, ISSN 0036-5513, E-ISSN 1502-7686, Vol. 68, no 4, p. 312-316Article in journal (Refereed) Published
Abstract [en]

The reliability of interpretations of findings from dip-slide devices for culturing urine was investigated in a national Swedish external quality assessment (EQA) programme. Also investigated was the extent of improvement in the examination procedure achieved through personnel training programmes and information. According to Swedish national recommendations, dip-slide should only be used in primary health care (PHC) in cases of uncomplicated urinary tract infection (UTI) in females of childbearing age. The recommendations also define six possible outcomes of a dip-slide examination, outcomes that have formed the basis for the EQA programme since 2001. No improvement in ability to classify readings correctly into the six categories was noted for the period 2001 to 2006. Preparations containing 'mixed flora' presented participants with the greatest difficulty, with only 28% correct reports. The EQA programme, with educational components and voluntary participation, has not improved quality. The disappointing results might be a reflection of the limited effort and resources allocated by clinical microbiology laboratories for training and for sustaining proficiency in the evaluation of dip-slides. For these reasons, we cannot at present recommend the dip-slide technique for use in PHC settings. © 2008 Informa UK Ltd (Informa Healthcare, Taylor & Francis AS).

National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-43406 (URN)10.1080/00365510701759703 (DOI)73760 (Local ID)73760 (Archive number)73760 (OAI)
Available from: 2009-10-10 Created: 2009-10-10 Last updated: 2017-12-13
Forsum, U. (2008). Ett område där värderingarna går isär. Läkartidningen, 105(43), 3058-3058
Open this publication in new window or tab >>Ett område där värderingarna går isär
2008 (Swedish)In: Läkartidningen, ISSN 0023-7205, E-ISSN 1652-7518, Vol. 105, no 43, p. 3058-3058Article in journal (Refereed) Published
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-44450 (URN)76654 (Local ID)76654 (Archive number)76654 (OAI)
Available from: 2009-10-10 Created: 2009-10-10 Last updated: 2017-12-13
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