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Gunnarsson, Patrik
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Bengtsson, T., Karlsson, H., Gunnarsson, P., Skoglund, C., Elison, C., Leanderson, P. & Lindahl, M. (2008). The periodontal pathogen Porphyromonas gingivalis cleaves apoB-100 and increases the expression of apoM in LDL in whole blood leading to cell proliferation. Journal of Internal Medicine, 263(5), 558-571
Open this publication in new window or tab >>The periodontal pathogen Porphyromonas gingivalis cleaves apoB-100 and increases the expression of apoM in LDL in whole blood leading to cell proliferation
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2008 (English)In: Journal of Internal Medicine, ISSN 0954-6820, E-ISSN 1365-2796, Vol. 263, no 5, p. 558-571Article in journal (Refereed) Published
Abstract [en]

Objective: Several studies support an association between periodontal disease and atherosclerosis with a crucial role for the pathogen Porphyromonas gingivalis. This study aims to investigate the proteolytic and oxidative activity of P. gingivalis on LDL in a whole blood system by using a proteomic approach and analyze the effects of P. gingivalis-modifed LDL on cell proliferation.

Methods: The cellular effects of P. gingivalis in human whole blood were assessed using lumi-aggregometry analyzing reactive oxygen species (ROS) production and aggregation. Blood was incubated for 30 min with P. gingivalis, whereafter LDL was isolated and a proteomic approach was applied to examine protein expression. LDL-oxidation was determined by analyzing the formation of protein carbonyls. The effects of P. gingivalis-modifed LDL on fibroblast proliferation were studied using the MTS-assay.

Results: Incubation of whole blood with P. gingivalis caused an extensive aggregation and ROS-production, indicating platelet and leukocyte activation. LDL prepared from the bacteria-exposed blood showed an increased protein oxidation, elevated levels of apoM and formation of two apoB-100 N-terminal fragments. P. gingivalis-modified LDL markedly increased the growth of fibroblasts. Inhibition of gingipain R suppressed the modification of LDL by P. gingivalis.

Conclusions: The ability of P. gingivalis to change the protein expression and the proliferative capacity of LDL may represent a crucial event in periodontitis-associated atherosclerosis.

Place, publisher, year, edition, pages
Institutionen för medicin och hälsa, 2008
Keywords
atherosclerosis, growth factors, infection, inflammation, lipoproteins
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-11870 (URN)10.1111/j.1365-2796.2007.01917.x (DOI)
Note

The definitive version is available at www.blackwell-synergy.com: Torbjörn Bengtsson, Helen Karlsson, Patrik Gunnarsson, Caroline Skoglund, Charlotte Elison, Per Leanderson and Mats Lindahl, The periodontal pathogen Porphyromonas gingivalis cleaves apoB-100 and increases the expression of apoM in LDL in whole blood leading to cell proliferation, 2008, Journal of Internal Medicine, (263), 5, 558-571. http://dx.doi.org/10.1111/j.1365-2796.2007.01917.x. Copyright: Blackwell Publishing www.blackwell-synergy.com

Available from: 2008-05-21 Created: 2008-05-21 Last updated: 2017-12-13Bibliographically approved
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