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Baumgardt, Magnus
Publications (10 of 14) Show all publications
Baumgardt, M., Karlsson, D., Yaghmaeian Salmani, B., Bivik, C., MacDonald, R., Gunnar, E. & Thor, S. (2014). Global Programmed Switch in Neural Daughter Cell Proliferation Mode Triggered by a Temporal Gene Cascade. Developmental Cell, 30(2), 192-208
Open this publication in new window or tab >>Global Programmed Switch in Neural Daughter Cell Proliferation Mode Triggered by a Temporal Gene Cascade
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2014 (English)In: Developmental Cell, ISSN 1534-5807, E-ISSN 1878-1551, Vol. 30, no 2, p. 192-208Article in journal (Refereed) Published
Abstract [en]

During central nervous system (CNS) development, progenitors typically divide asymmetrically, renewing themselves while budding off daughter cells with more limited proliferative potential. Variation in daughter cell proliferation has a profound impact on CNS development and evolution, but the underlying mechanisms remain poorly understood. We find that Drosophila embryonic neural progenitors (neuroblasts) undergo a programmed daughter proliferation mode switch, from generating daughters that divide once (type I) to generating neurons directly (type 0). This typelgreater than0 switch is triggered by activation of Dacapo (mammalian p21(CIP1)/p27(KIP1)/p57(Kip2)) expression in neuroblasts. In the thoracic region, Dacapo expression is activated by the temporal cascade (castor) and the Hox gene Antennapedia. In addition, castor, Antennapedia, and the late temporal gene grainyhead act combinatorially to control the precise timing of neuroblast cell-cycle exit by repressing Cyclin E and E2f. This reveals a logical principle underlying progenitor and daughter cell proliferation control in the Drosophila CNS.

Place, publisher, year, edition, pages
Elsevier (Cell Press), 2014
National Category
Basic Medicine
Identifiers
urn:nbn:se:liu:diva-109588 (URN)10.1016/j.devcel.2014.06.021 (DOI)000339641500012 ()25073156 (PubMedID)
Available from: 2014-08-21 Created: 2014-08-21 Last updated: 2019-03-13
Ulvklo, C., MacDonald, R., Bivik, C., Baumgardt, M., Karlsson, D. & Thor, S. (2012). Control of neuronal cell fate and number by integration of distinct daughter cell proliferation modes with temporal progression. Development, 139(4), 678-689
Open this publication in new window or tab >>Control of neuronal cell fate and number by integration of distinct daughter cell proliferation modes with temporal progression
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2012 (English)In: Development, ISSN 0950-1991, E-ISSN 1477-9129, Vol. 139, no 4, p. 678-689Article in journal (Refereed) Published
Abstract [en]

During neural lineage progression, differences in daughter cell proliferation can generate different lineage topologies. This is apparent in the Drosophila neuroblast 5-6 lineage (NB5-6T), which undergoes a daughter cell proliferation switch from generating daughter cells that divide once to generating neurons directly. Simultaneously, neural lineages, e.g. NB5-6T, undergo temporal changes in competence, as evidenced by the generation of different neural subtypes at distinct time points. When daughter proliferation is altered against a backdrop of temporal competence changes, it may create an integrative mechanism for simultaneously controlling cell fate and number. Here, we identify two independent pathways, Prospero and Notch, which act in concert to control the different daughter cell proliferation modes in NB5-6T. Altering daughter cell proliferation and temporal progression, individually and simultaneously, results in predictable changes in cell fate and number. This demonstrates that different daughter cell proliferation modes can be integrated with temporal competence changes, and suggests a novel mechanism for coordinately controlling neuronal subtype numbers.

Place, publisher, year, edition, pages
Company of Biologists, 2012
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-74790 (URN)10.1242/dev.074500 (DOI)000300259800005 ()
Note

funding agencies|Swedish Research Council||Knut and Alice Wallenberg foundation||Swedish Cancer Foundation||

Available from: 2012-02-08 Created: 2012-02-08 Last updated: 2019-03-13
MacDonald, R., Ulvklo, C., Bivik, C., Baumgardt, M., Karlsson, D. & Thor, S. (2011). Notch Mediates a Genetic Switch in Neural Lineage Topology in DEVELOPMENTAL BIOLOGY, vol 356, issue 1, pp 227-227. In: DEVELOPMENTAL BIOLOGY: (pp. 227-227). Elsevier Science B.V., Amsterdam, 356(1)
Open this publication in new window or tab >>Notch Mediates a Genetic Switch in Neural Lineage Topology in DEVELOPMENTAL BIOLOGY, vol 356, issue 1, pp 227-227
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2011 (English)In: DEVELOPMENTAL BIOLOGY, Elsevier Science B.V., Amsterdam , 2011, Vol. 356, no 1, p. 227-227Conference paper, Published paper (Refereed)
Abstract [en]

n/a

Place, publisher, year, edition, pages
Elsevier Science B.V., Amsterdam, 2011
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-69781 (URN)10.1016/j.ydbio.2011.05.375 (DOI)000292784400400 ()
Available from: 2011-08-10 Created: 2011-08-08 Last updated: 2019-03-13
Benito-Sipos, J., Ulvklo, C., Gabilondo, H., Baumgardt, M., Angel, A., Torroja, L. & Thor, S. (2011). Seven up acts as a temporal factor during two different stages of neuroblast 5-6 development. Development, 138(24), 5311-5320
Open this publication in new window or tab >>Seven up acts as a temporal factor during two different stages of neuroblast 5-6 development
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2011 (English)In: Development, ISSN 0950-1991, E-ISSN 1477-9129, Vol. 138, no 24, p. 5311-5320Article in journal (Refereed) Published
Abstract [en]

Drosophila embryonic neuroblasts generate different cell types at different time points. This is controlled by a temporal cascade of Hb -greater than Kr -greater than Pdm -greater than Cas -greater than Grh, which acts to dictate distinct competence windows sequentially. In addition, Seven up (Svp), a member of the nuclear hormone receptor family, acts early in the temporal cascade, to ensure the transition from Hb to Kr, and has been referred to as a switching factor. However, Svp is also expressed in a second wave within the developing CNS, but here, the possible role of Svp has not been previously addressed. In a genetic screen for mutants affecting the last-born cell in the embryonic NB5-6T lineage, the Ap4/FMRFamide neuron, we have isolated a novel allele of svp. Expression analysis shows that Svp is expressed in two distinct pulses in NB5-6T, and mutant analysis reveals that svp plays two distinct roles. In the first pulse, svp acts to ensure proper downregulation of Hb. In the second pulse, which occurs in a Cas/Grh double-positive window, svp acts to ensure proper sub-division of this window. These studies show that a temporal factor may play dual roles, acting at two different stages during the development of one neural lineage.

Place, publisher, year, edition, pages
Company of Biologists, 2011
Keywords
Temporal genes; COUP-TFI/II; Cell specification; Lineage progression; Drosophila
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-73311 (URN)10.1242/dev.070946 (DOI)297329900004 ()
Available from: 2012-01-03 Created: 2012-01-02 Last updated: 2017-12-08Bibliographically approved
Benito-Sipos, J., Estacio-Gomez, A., Moris-Sanz, M., Baumgardt, M., Thor, S. & J Diaz-Benjumea, F. (2010). A genetic cascade involving klumpfuss, nab and castor specifies the abdominal leucokinergic neurons in the Drosophila CNS. DEVELOPMENT, 137(19), 3327-3336
Open this publication in new window or tab >>A genetic cascade involving klumpfuss, nab and castor specifies the abdominal leucokinergic neurons in the Drosophila CNS
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2010 (English)In: DEVELOPMENT, ISSN 0950-1991, Vol. 137, no 19, p. 3327-3336Article in journal (Refereed) Published
Abstract [en]

Identification of the genetic mechanisms underlying the specification of large numbers of different neuronal cell fates from limited numbers of progenitor cells is at the forefront of developmental neurobiology. In Drosophila, the identities of the different neuronal progenitor cells, the neuroblasts, are specified by a combination of spatial cues. These cues are integrated with temporal competence transitions within each neuroblast to give rise to a specific repertoire of cell types within each lineage. However, the nature of this integration is poorly understood. To begin addressing this issue, we analyze the specification of a small set of peptidergic cells: the abdominal leucokinergic neurons. We identify the progenitors of these neurons, the temporal window in which they are specified and the influence of the Notch signaling pathway on their specification. We also show that the products of the genes klumpfuss, nab and castor play important roles in their specification via a genetic cascade.

Place, publisher, year, edition, pages
Company of Biologists Ltd, 2010
Keywords
Drosophila, CNS development, Neuronal fate specification, Leucokinin, ABLK
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-59724 (URN)10.1242/dev.052233 (DOI)000281608200018 ()
Available from: 2010-09-24 Created: 2010-09-24 Last updated: 2016-11-30
Thor, S., Baumgardt, M. & Karlsson, D. (2010). From progenitor to unique neuron Neuronal sub-type specification by the integration of positional and temporal cues in INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE, vol 28, issue 8, pp 671-671. In: INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE (pp. 671-671). Elsevier Science B.V., Amsterdam., 28(8)
Open this publication in new window or tab >>From progenitor to unique neuron Neuronal sub-type specification by the integration of positional and temporal cues in INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE, vol 28, issue 8, pp 671-671
2010 (English)In: INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE, Elsevier Science B.V., Amsterdam. , 2010, Vol. 28, no 8, p. 671-671Conference paper, Published paper (Refereed)
Abstract [en]

n/a

Place, publisher, year, edition, pages
Elsevier Science B.V., Amsterdam., 2010
Keywords
Cell specification, Neuronal sub-types, Progenitor identity, Positional and temporal cues
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-64765 (URN)10.1016/j.ijdevneu.2010.07.088 (DOI)000284967600085 ()
Available from: 2011-02-04 Created: 2011-02-04 Last updated: 2016-11-30
Losada-Perez, M., Gabilondo, H., del Saz, D., Baumgardt, M., Molina, I., Leon, Y., . . . Benito-Sipos, J. (2010). Lineage-unrelated neurons generated in different temporal windows and expressing different combinatorial codes can converge in the activation of the same terminal differentiation gene in JOURNAL OF NEUROGENETICS, vol 24, issue , pp 80-80. In: JOURNAL OF NEUROGENETICS (pp. 80-80). Informa Healthcare, 24
Open this publication in new window or tab >>Lineage-unrelated neurons generated in different temporal windows and expressing different combinatorial codes can converge in the activation of the same terminal differentiation gene in JOURNAL OF NEUROGENETICS, vol 24, issue , pp 80-80
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2010 (English)In: JOURNAL OF NEUROGENETICS, Informa Healthcare , 2010, Vol. 24, p. 80-80Conference paper, Published paper (Refereed)
Abstract [en]

n/a

Place, publisher, year, edition, pages
Informa Healthcare, 2010
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-65940 (URN)000284537500211 ()
Available from: 2011-02-28 Created: 2011-02-28 Last updated: 2011-02-28
Karlsson, D., Baumgardt, M. & Thor, S. (2010). Segment-specific Neuronal Sub-type Specification by the Integration of Anteroposterior and Temporal Cues. PLoS biology, 8(5)
Open this publication in new window or tab >>Segment-specific Neuronal Sub-type Specification by the Integration of Anteroposterior and Temporal Cues
2010 (English)In: PLoS biology, ISSN 1544-9173, E-ISSN 1545-7885, Vol. 8, no 5Article in journal (Refereed) Published
Abstract [en]

The generation of distinct neuronal sub-types at different axial levels relies upon both anteroposterior and temporal cues. However, the integration between these cues is poorly understood. In the Drosophila CNS, the segmentally repeated neuroblast 5-6 generates a unique group of neurons, the Apterous cluster, only in thoracic segments. Recent studies have identified elaborate genetic pathways acting to control the generation of these neurons. These insights, combined with novel markers, provide a unique opportunity for addressing how anteroposterior and temporal cues are integrated to generate segment-specific neuronal sub-types. We find that Pbx/Meis, Hox and temporal genes act in three different ways. Posteriorly, Pbx/Meis and posterior Hox genes block lineage progression within an early temporal window, by triggering cell cycle exit. Because Ap neurons are generated late in the thoracic 5-6 lineage, this prevents generation of Ap cluster cells in the abdomen. Thoracically, Pbx/Meis and anterior Hox genes integrate with late temporal genes to specify Ap clusters, via activation of a specific feed-forward loop. In brain segments, ‘Ap cluster cells’ are present but lack both proper Hox and temporal coding. Only by simultaneously altering Hox and temporal gene activity in all segments can Ap clusters be generated throughout the neuroaxis. This study provides the first detailed analysis of an identified neuroblast lineage along the entire neuroaxis, and provides novel insight into how Hox/Pbx/Meis anteroposterior cues are integrated with temporal cues. It reveals a surprisingly restricted yet multifaceted function of the anteroposterior cues with respect to lineage control and cell fate specification.

Keywords
anteroposterior patterning, temporal transitions, Hox, Pbx/Meis, cell specification
National Category
Developmental Biology
Identifiers
urn:nbn:se:liu:diva-51641 (URN)10.1371/journal.pbio.1000368 (DOI)000278759600005 ()
Note
Original Publication: Daniel Karlsson, Magnus Baumgardt and Stefan Thor, Segment-specific Neuronal Sub-type Specification by the Integration of Anteroposterior and Temporal Cues, 2010, PLoS biology, (8), 5. http://dx.doi.org/10.1371/journal.pbio.1000368 Licensee: Public Library of Science http://www.plos.org/ Available from: 2009-11-11 Created: 2009-11-11 Last updated: 2017-12-12Bibliographically approved
Benito-Sipos, J., Estacio, A., Moris, M., Baumgardt, M., Thor, S. & Diaz-Benjumea , F. J. (2009). Analysis of the specification of the Leucokininergic cell fate. In: The 12th European Drosophila Neurobiology Conference 6-10 September 2008 Wuerzburg, Germany: in: Journal of Neurogenetics, Volume 23 Supplement 1 2009 (pp. S16-S16). , 23
Open this publication in new window or tab >>Analysis of the specification of the Leucokininergic cell fate
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2009 (English)In: The 12th European Drosophila Neurobiology Conference 6-10 September 2008 Wuerzburg, Germany: in: Journal of Neurogenetics, Volume 23 Supplement 1 2009, 2009, Vol. 23, p. S16-S16Conference paper, Published paper (Refereed)
Identifiers
urn:nbn:se:liu:diva-17150 (URN)
Available from: 2009-03-07 Created: 2009-03-07 Last updated: 2016-11-30
Baumgardt, M., Karlsson, D., Terriente, J., J Diaz-Benjumea, F. & Thor , S. (2009). From stem cell to unique neuron: Temporal transitions in an identified CNS progenitor cell by feedforward combinatorial coding. In: The 12th European Drosophila Neurobiology Conference 6-10 September 2008 Wuerzburg, Germany: in: Journal of Neurogenetics, Volume 23 Supplement 1 2009 (pp. S14-S15). , 23
Open this publication in new window or tab >>From stem cell to unique neuron: Temporal transitions in an identified CNS progenitor cell by feedforward combinatorial coding
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2009 (English)In: The 12th European Drosophila Neurobiology Conference 6-10 September 2008 Wuerzburg, Germany: in: Journal of Neurogenetics, Volume 23 Supplement 1 2009, 2009, Vol. 23, p. S14-S15Conference paper, Published paper (Refereed)
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-17149 (URN)
Available from: 2009-03-07 Created: 2009-03-07 Last updated: 2016-11-30
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