liu.seSearch for publications in DiVA
Change search
Link to record
Permanent link

Direct link
BETA
Lundqvist Setterud, Helen
Alternative names
Publications (10 of 13) Show all publications
Hård af Segerstad, H., Setterud, H. & Salerud, G. (2008). An alternative supervision model of Master thesis. In: : . Paper presented at International Consortium for Educational Development (ICED), 12-15 June 2008, Salt Lake City, Utah, USA.
Open this publication in new window or tab >>An alternative supervision model of Master thesis
2008 (English)Conference paper, Oral presentation with published abstract (Other academic)
Keywords
Supervision, master thesis
National Category
Medical Engineering
Identifiers
urn:nbn:se:liu:diva-108589 (URN)
Conference
International Consortium for Educational Development (ICED), 12-15 June 2008, Salt Lake City, Utah, USA
Available from: 2014-06-30 Created: 2014-06-30 Last updated: 2014-08-29
Bjuremark, A., Setterud, H. & Franzén, C. (2008). Att examinera kvalitet i kurser och program. Linköping: Linköping Univeristy Electronic Press
Open this publication in new window or tab >>Att examinera kvalitet i kurser och program
2008 (Swedish)Report (Other academic)
Alternative title[sv]
Ingår i rapporten: Variation på temat examination : En rapport från grundutbildningsdag och rundabordssamtal vid LiU 2007
Abstract [sv]

En central fråga under rundabordssamtalen var om det fanns några tips på examinationsförfaranden som skulle vara särskilt stimulerande för lärandet. Det praktiska råden var dock få och istället lyftes lärarens roll och kompetens fram som vägvisare för den kunskap som studenterna förväntades tillägna sig.Vid sidan av ‘nytta’ visade sig även kriterier ‘framtidens krav’ vara centralt

Place, publisher, year, edition, pages
Linköping: Linköping Univeristy Electronic Press, 2008. p. 3
Series
CUL-rapporter, ISSN 1652-9278 ; 2008:13
Keywords
Kursexamination, programexamination
National Category
Social Sciences
Identifiers
urn:nbn:se:liu:diva-44847 (URN)77828 (Local ID)978-91-7393-719-1 (ISBN)77828 (Archive number)77828 (OAI)
Available from: 2009-10-10 Created: 2009-10-10 Last updated: 2011-03-09Bibliographically approved
Hård af Segerstad, H., Setterud, H. & Salerud, G. (2008). Master students and supervisors’ conceptions and experiences of an alternative model of supervision. In: : . Paper presented at AARE 2008, International Education Research Conference, 30 November - 4 December 2008, Brisbane, Australia.
Open this publication in new window or tab >>Master students and supervisors’ conceptions and experiences of an alternative model of supervision
2008 (English)Conference paper, Oral presentation with published abstract (Other academic)
Keywords
Master thesis, supervision
National Category
Medical Engineering
Identifiers
urn:nbn:se:liu:diva-108590 (URN)
Conference
AARE 2008, International Education Research Conference, 30 November - 4 December 2008, Brisbane, Australia
Available from: 2014-06-30 Created: 2014-06-30 Last updated: 2014-08-29
Särndahl, E., Bergström, I., Brodin Patcha, V., Nijm, J., Setterud, H. & Jonasson, L. (2007). Activation state of neutrophils in patients with stable coronary artery disease. In: 76th Congress of the European Atherosclerosis Society,2007.
Open this publication in new window or tab >>Activation state of neutrophils in patients with stable coronary artery disease
Show others...
2007 (English)In: 76th Congress of the European Atherosclerosis Society,2007, 2007Conference paper, Published paper (Other academic)
Abstract [en]

   

National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-40760 (URN)54061 (Local ID)54061 (Archive number)54061 (OAI)
Available from: 2009-10-10 Created: 2009-10-10
Särndahl, E., Bergström, I., Brodin, V. P., Nijm, J., Lundqvist Setterud, H. & Jonasson, L. (2007). Neutrophil activation status in stable coronary artery disease.. PLoS ONE, 2(10), e1056-
Open this publication in new window or tab >>Neutrophil activation status in stable coronary artery disease.
Show others...
2007 (English)In: PLoS ONE, ISSN 1932-6203, Vol. 2, no 10, p. e1056-Article in journal (Refereed) Published
Abstract [en]

Background: During the last years, neutrophils have emerged as important players in atherogenesis. They are highly activated in peripheral blood of patients with unstable angina. Moreover, a primed state of circulating neutrophils has been proposed in patients with stable angina. Our aim was to investigate the neutrophil activation status in patients with stable coronary artery disease (CAD) at conventional drug treatment.

Methodology and principal findings: Thirty patients with stable CAD and 30 healthy controls were included using a paired design. The neutrophil expression of CD18 and high-affinity state of CD11b was analysed by flow cytometry before and after stimulation with chemoattractants. Also, the production of reactive oxygen species (ROS) was determined by chemiluminescence. During basal conditions, the neutrophil expression of CD18 or high-affinity state of CD11b did not differ between patients and controls. Chemoattractants (Interleukin-8 and Leukotriene B(4)) did not increase either the expression or the amount of high-affinity CD11b/CD18-integrins in CAD patients compared to controls, and had no effect on the production of ROS. On the other hand, the ROS production in response to C3bi-opsonised yeast particles and the neutrophils' inherent capacity to produce ROS were both significantly decreased in patients.

Conclusion/Significance: We could not find any evidence that neutrophils in patients with stable CAD were primed, i.e. more prone to activation, compared to cells from healthy controls. According to our data, the circulating neutrophils in CAD patients rather showed an impaired activation status. It remains to be elucidated whether the neutrophil dysfunction in CAD is mainly a marker of chronic disease, an atherogenic factor or a consequence of the drug treatment.

National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-17246 (URN)10.1371/journal.pone.0001056 (DOI)17957240 (PubMedID)
Available from: 2009-03-12 Created: 2009-03-12 Last updated: 2010-01-14
Nilsdotter-Augustinsson, Å., Claesson, C., Lindgren, P.-E., Lundqvist Gustafsson, H. & Öhman, L. (2005). Adherence of Staphylococcus epidermidis to extracellular matrix proteins and effects of fibrinogen-bound bacteria on oxidase activity and apoptosis in neutrophils. Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), 113(5), 361-373
Open this publication in new window or tab >>Adherence of Staphylococcus epidermidis to extracellular matrix proteins and effects of fibrinogen-bound bacteria on oxidase activity and apoptosis in neutrophils
Show others...
2005 (English)In: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), ISSN 0903-4641, E-ISSN 1600-0463, Vol. 113, no 5, p. 361-373Article in journal (Refereed) Published
Abstract [en]

Staphylococcus epidermidis often causes foreign-body infections such as those associated with hip prostheses, but the underlying pathogenic mechanisms are not fully understood. We performed spectrophotometry to study the ability of S. epidermidis to bind to immobilised fibrinogen, fibronectin, vitronectin, and collagen. The strains were isolated from infected hip prostheses or from normal flora and the well-known protein-binding strain Staphylococcus aureus Cowan was used as positive control. We also analysed the interaction between neutrophils and a fibrinogen-bound prosthesis-derived strain of S. epidermidisby measuring chemiluminescence to determine the neutrophil oxidative response and binding of annexin V to indicate neutrophil apoptosis. We found that binding of S. epidermidis to extracellular matrix proteins varied under different growth conditions, and that prosthesis isolates adhered better to vitronectin than did strains from normal flora. The oxidative response caused by fibrinogen-bound S. epidermidis was not above the background level, which was in marked contrast to the distinct response induced by fibrinogen-associated S. aureus Cowan. Furthermore, fibrinogen-adhering S. epidermidis retarded neutrophil apoptosis. We conclude that surface-bound S. epidermidis induces only a weak inflammatory response, which in combination with the ability of the adherent bacteria to retard neutrophil apoptosis may contribute to low-grade inflammation and loosening of prostheses.

National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-28719 (URN)10.1111/j.1600-0463.2005.apm_113508.x (DOI)13888 (Local ID)13888 (Archive number)13888 (OAI)
Available from: 2009-10-09 Created: 2009-10-09 Last updated: 2017-12-13Bibliographically approved
Larsson, J., Persson, C., Tengvall, P. & Lundqvist Gustafsson, H. (2004). Anti-inflammatory effects of a titanium-peroxy gel: role of oxygen metabolites and apoptosis. Journal of Biomedical Materials Research, 68(3), 448-457
Open this publication in new window or tab >>Anti-inflammatory effects of a titanium-peroxy gel: role of oxygen metabolites and apoptosis
2004 (English)In: Journal of Biomedical Materials Research, ISSN 0021-9304, E-ISSN 1097-4636, Vol. 68, no 3, p. 448-457Article in journal (Refereed) Published
Abstract [en]

Polymorphonuclear neutrophils (PMN) are among the first inflammatory cells to arrive at an implant interface, where they encounter with the foreign material and may produce reactive oxygen species (ROS). During the interaction between titanium and ROS, titanium-peroxy (Ti-peroxy) compounds may be formed. We used a Ti-peroxy gel, made from titanium and hydrogen peroxide, to study the effects of Ti-peroxy compounds on PMN. In the absence of serum, the Ti-peroxy gel decreased the oxidative response of PMN to yeast and PMA and reduced PMN apoptosis without inducing necrosis. These effects could not be ascribed to the release of hydrogen peroxide from the Ti-peroxy gel, because a steady-state hydrogen peroxide producing system failed to mimic the effects of the gel. The effects were similarly unaffected when PMN were preincubated with β2-integrin antibodies, questioning the involvement of adhesion molecules. Nevertheless, when a filter was used to separate the Ti-peroxy gel from the cells, the gel effect on PMN life span was abolished, pointing to a contact-dependent mechanism. In the presence of serum, the Ti-peroxy gel had no effect on the PMN oxidative response and life span, but appeared rather inert. In summary, this study demonstrates that the Ti-peroxy gel has potentially anti-inflammatory properties through a combined peroxide and physical contact effect, supporting the notion that interactions between titanium and inflammatory cells are responsible for the good performance of titanium in vivo.

National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-23785 (URN)10.1002/jbm.a.20078 (DOI)3302 (Local ID)3302 (Archive number)3302 (OAI)
Available from: 2009-10-07 Created: 2009-10-07 Last updated: 2017-12-13Bibliographically approved
Nilsdotter-Augustinsson, Å., Wilsson, Å., Larsson, J., Stendahl, O., Öhman, L. & Lundqvist Gustafsson, H. (2004). Staphylococcus aureus, but not Staphylococcus epidermidis, modulates the oxidative response and induces apoptosis in human neutrophils. Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), 112(2), 109-118
Open this publication in new window or tab >>Staphylococcus aureus, but not Staphylococcus epidermidis, modulates the oxidative response and induces apoptosis in human neutrophils
Show others...
2004 (English)In: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), ISSN 0903-4641, E-ISSN 1600-0463, Vol. 112, no 2, p. 109-118Article in journal (Refereed) Published
Abstract [en]

S. epidermidis is the most common isolate in foreign body infections. The aim of this study was to understand why S. epidermidis causes silent biomaterial infections. In view of the divergent inflammatory responses S. epidermidis and S. aureus cause in patients, we analyzed how they differ when interacting with human neutrophils. Neutrophils interacting with S. epidermidis strains isolated either from granulation tissue covering infected hip prostheses or from normal skin flora were tested by measuring the oxidative response as chemiluminescence and apoptosis as annexin V binding. Different S. aureus strains were tested in parallel. All S. epidermidis tested were unable to modulate the oxidative reaction in response to formyl-methionyl-leucyl-phenylalanine (fMLP) and did not provoke, but rather inhibited, apoptosis. In contrast, some S. aureus strains enhanced the oxidative reaction, and this priming capacity was linked to p38-mitogen-activated-protein-kinase (p38-MAPK) activation and induction of apoptosis. Our results may explain why S. epidermidis is a weak inducer of inflammation compared to S. aureus, and therefore responsible for the indolent and chronic course of S. epidermidis biomaterial infections.

National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-23732 (URN)10.1111/j.1600-0463.2004.apm1120205.x (DOI)3239 (Local ID)3239 (Archive number)3239 (OAI)
Available from: 2009-10-07 Created: 2009-10-07 Last updated: 2017-12-13Bibliographically approved
Lundqvist-Gustafsson, H., Norrman, S., Nilsson, J. & Wilsson, Å. (2001). Involvement of p38-mitogen-activated protein kinase in staphylococcus aureus-induced neutrophil apoptosis. Journal of Leukocyte Biology, 70(4), 642-648
Open this publication in new window or tab >>Involvement of p38-mitogen-activated protein kinase in staphylococcus aureus-induced neutrophil apoptosis
2001 (English)In: Journal of Leukocyte Biology, ISSN 0741-5400, E-ISSN 1938-3673, Vol. 70, no 4, p. 642-648Article in journal (Refereed) Published
Abstract [en]

Apoptosis occurred in human neutrophils within an hour of exposure to viable serumopsonized Staphylococcus aureus, as indicated by appearance of cells with condensed nuclei, fragmented DNA, and increased phosphatidylserine exposure. In contrast, serum-opsomized, heat-killed S. aureus did not induce apoptosis. This discrepancy could not be explained by differences in bacterial uptake or total NADPH-oxidase activity. Suppressing phagocytosis by pretreating the neutrophils with cytochalasin b or by using nonopsonized bacteria did not prevent apoptosis. A supernatant from bacteria grown for 2 h in nutrient broth had a strong proapoptotic influence that was abrogated by heat treatment. Exposure to viable S. aureus or supernatant also led to activation of p38-mitogen-activated protein kinase in the neutrophils. Inhibition of this kinase with SB203580 reduced the apoptosis-inducing capacity of both bacteria and supernatant. We conclude that S. aureus activates p38-mitogen-activated protein kinase in neutrophils and induces apoptosis, probably mediated by a bacteria-derived soluble factor(s).

National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-27857 (URN)12617 (Local ID)12617 (Archive number)12617 (OAI)
Available from: 2009-10-08 Created: 2009-10-08 Last updated: 2017-12-13Bibliographically approved
Lundqvist-Gustafsson, H., Gustafsson, M. & Dahlgren, C. (2000). Dynamic Ca2+ changes in neutrophil phagosomes. A source for intracellular Ca2+ during phagolysosome formation?. Cell Calcium, 27(6), 353-362
Open this publication in new window or tab >>Dynamic Ca2+ changes in neutrophil phagosomes. A source for intracellular Ca2+ during phagolysosome formation?
2000 (English)In: Cell Calcium, ISSN 0143-4160, E-ISSN 1532-1991, Vol. 27, no 6, p. 353-362Article in journal (Refereed) Published
Abstract [en]

An increase in cytosolic Ca2+ concentration periphagosomally is critical for phagolysosomal formation and neutrophil elimination of microbes. The Ca2+ increase could be achieved through release of Ca2+ from mobilized intracellular stores. Alternatively, Ca2+ that passively enter the phagosome during phagocytosis could be provided by the phagosome. Intraphagosomal Ca2+ changes in single human neutrophils was measured during phagocytosis of serum opsonized Fura-2-conjugated zymosan particles, using a digital image processing system for microspectrofluorometry. A decrease in phagosomal Ca2+ down to nanomolar concentrations was seen within minutes following phagosomal closure. Blockage of plasma membrane Ca2+ channels by econazole abolished this decrease. The fluorescence properties of Fura-2 zymosan were retained after phagocytosis and stable to pH changes, reactive oxygen species, and proteolytic enzymes. We suggest that Ca2+ ions present in the phagosome enter the cell cytosol through Ca2+ channels in the phagosomal membrane, achieving a localized Ca2+ rise that is important for phagosome processing.

National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-27858 (URN)10.1054/ceca.2000.0130 (DOI)12618 (Local ID)12618 (Archive number)12618 (OAI)
Available from: 2009-10-08 Created: 2009-10-08 Last updated: 2017-12-13
Organisations

Search in DiVA

Show all publications