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Tagesson, Christer
Alternative names
Publications (10 of 41) Show all publications
Ghafouri, B., Carlsson, A., Holmberg, S., Thelin, A. & Tagesson, C. (2016). Biomarkers of lsystemic inflammation in farmers with musculoskeletal disorders; a plasma proteomic study. BMC Musculoskeletal Disorders, 17(206)
Open this publication in new window or tab >>Biomarkers of lsystemic inflammation in farmers with musculoskeletal disorders; a plasma proteomic study
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2016 (English)In: BMC Musculoskeletal Disorders, ISSN 1471-2474, E-ISSN 1471-2474, Vol. 17, no 206Article in journal (Refereed) Published
Abstract [en]

Background: Farmers have an increased risk for musculoskeletal disorders (MSD) such as osteoarthritis of the hip, low back pain, and neck and upper limb complaints. The underlying mechanisms are not fully understood. Workrelated exposures and inflammatory responses might be involved. Our objective was to identify plasma proteins that differentiated farmers with MSD from rural referents. Methods: Plasma samples from 13 farmers with MSD and rural referents were included in the investigation. Gel based proteomics was used for protein analysis and proteins that differed significantly between the groups were identified by mass spectrometry. Results: In total, 15 proteins differed significantly between the groups. The levels of leucine-rich alpha-2-glycoprotein, haptoglobin, complement factor B, serotransferrin, one isoform of kininogen, one isoform of alpha-1-antitrypsin, and two isoforms of hemopexin were higher in farmers with MSD than in referents. On the other hand, the levels of alpha-2-HS-glycoprotein, alpha-1B-glycoprotein, vitamin D-binding protein, apolipoprotein A1, antithrombin, one isoform of kininogen, and one isoform of alpha-1-antitrypsin were lower in farmers than in referents. Many of the identified proteins are known to be involved in inflammation. Conclusions: Farmers with MSD had altered plasma levels of protein biomarkers compared to the referents, indicating that farmers with MSD may be subject to a more systemic inflammation. It is possible that the identified differences of proteins may give clues to the biochemical changes occurring during the development and progression of MSD in farmers, and that one or several of these protein biomarkers might eventually be used to identify and prevent work-related MSD.

Place, publisher, year, edition, pages
BIOMED CENTRAL LTD, 2016
Keywords
Musculoskeletal disorder; Farmers; Proteomic; Systemic inflammation; Occupational medicine
National Category
Basic Medicine
Identifiers
urn:nbn:se:liu:diva-128936 (URN)10.1186/s12891-016-1059-y (DOI)000375761500001 ()27160764 (PubMedID)
Note

Funding Agencies|Swedish Farmers Foundation for Agricultural Research [H0935072]; Swedish Rheumatism Association [R-420491]

Available from: 2016-06-09 Created: 2016-06-07 Last updated: 2018-01-10
Ghafouri, B., Persson, H. L. & Tagesson, C. (2013). Intriguing bronchoalveolar lavage proteome in a case of pulmonary langerhans cell histiocytosis. The American journal of case reports, 14, 129-133
Open this publication in new window or tab >>Intriguing bronchoalveolar lavage proteome in a case of pulmonary langerhans cell histiocytosis
2013 (English)In: The American journal of case reports, ISSN 1941-5923, Vol. 14, p. 129-133Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: Pulmonary Langerhans cell histiocytosis (PLCH) is a rare interstitial lung disease associated with tobacco smoke exposure. New insights into its pathogenesis and how it differs from that of chronic obstructive pulmonary disease (COPD) may be provided by proteomic studies on bronchoalveolar lavage fluid (BALF).

CASE REPORT: We present the BALF proteome in a biopsy-proven case of PLCH and compare it with typical proteomes of COPD and of the healthy lung. The BALF proteins were separated by two-dimensional gel electrophoresis (2-DE) and the protein patterns were analyzed with a computerized 2-DE imaging system. As compared to the healthy subject and the COPD case, the PLCH case showed a strikingly different 2-DE pattern. There was much more IgG (heavy chain) and orosomucoid, and less α1-antitrypsin, surfactant protein-A, haptoglobin, cystatin-S, Clara cell protein 10, transthyretin and gelsolin. Moreover, no apolipoprotein-A1, pro-apolipoprotein-A1, amyloid P, calgranulin A, or calgranulin B was detected at all.

CONCLUSIONS: This case of PLCH presents with an extreme BALF proteome lacking significant amounts of protective and anti-inflammatory proteins. Thus, the intriguing BALF proteome opens up new lines of research into the pathophysiology of PLCH and how its pathogenesis differs from that in COPD.

Keywords
BALF, COPD, Inflammation, Lung, tobacco smoke
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-103740 (URN)10.12659/AJCR.889037 (DOI)23875058 (PubMedID)
Available from: 2014-01-24 Created: 2014-01-24 Last updated: 2014-02-11Bibliographically approved
Wieslander, G., Fabjan, N., Vogrincic, M., Kreft, I., Janson, C., Spetz-Nystrom, U., . . . Norback, D. (2011). Eating Buckwheat Cookies Is Associated with the Reduction in Serum Levels of Myeloperoxidase and Cholesterol: A Double Blind Crossover Study in Day-Care Centre Staffs. Tohoku journal of experimental medicine, 225(2), 123-130
Open this publication in new window or tab >>Eating Buckwheat Cookies Is Associated with the Reduction in Serum Levels of Myeloperoxidase and Cholesterol: A Double Blind Crossover Study in Day-Care Centre Staffs
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2011 (English)In: Tohoku journal of experimental medicine, ISSN 0040-8727, E-ISSN 1349-3329, Vol. 225, no 2, p. 123-130Article in journal (Refereed) Published
Abstract [en]

Buckwheat food is a good source of antioxidants, e.g. rutin, and other beneficial substances. Here we investigated the effects of the intake of common buckwheat (low rutin content) and tartary buckwheat cookies (high rutin content) on selected clinical markers. A double blind crossover study was performed among female day-care centre staffs (N = 62) from five day-care centres. Participants were randomly divided into two groups. The first group initially consumed four common buckwheat cookies per day (16.5 mg rutin equivalents/day) for two weeks, while the second group consumed four tartary buckwheat cookies per day (359.7 mg rutin equivalents/day). Then the groups switched their type of cookies and consumed them for another two weeks. We monitored selected clinical markers related to cardiovascular disease and lower airway inflammation, lung function, and subjective breathing difficulties in the staffs. Intake of tartary buckwheat cookies reduced the serum level of myeloperoxidase (MPO) by a factor 0.84 (p = 0.02). When grouping the two types of buckwheat cookies together, there was a reduction of total serum cholesterol (p andlt; 0.001) and HDL-cholesterol (p andlt; 0.001) during the study period, with improved lung vital capacity (p andlt; 0.001). The degree of reduction in total and HDL cholesterol levels was similar in staffs with low and high body mass index (cut off 25). In conclusion, intake of tartary buckwheat cookies with high level of the antioxidant rutin may reduce levels of MPO, an indicator of inflammation. Moreover, intake of both types of buckwheat cookies may lower cholesterol levels.

Place, publisher, year, edition, pages
Tohoku University Medical Press, 2011
Keywords
antioxidant experiment, buckwheat, cholesterol, inflammation, lung function
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-71794 (URN)10.1620/tjem.225.123 (DOI)000295498500009 ()
Available from: 2011-11-04 Created: 2011-11-04 Last updated: 2017-12-08
Fornander, L., Ghafouri, B., Kihlström, E., Åkerlind, B., Schön, T., Tagesson, C. & Lindahl, M. (2011). Innate immunity proteins and a new truncated form of SPLUNC1 in nasopharyngeal aspirates from infants with respiratory syncytial virus infection. PROTEOMICS CLINICAL APPLICATIONS, 5(9-10), 513-522
Open this publication in new window or tab >>Innate immunity proteins and a new truncated form of SPLUNC1 in nasopharyngeal aspirates from infants with respiratory syncytial virus infection
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2011 (English)In: PROTEOMICS CLINICAL APPLICATIONS, ISSN 1862-8346, Vol. 5, no 9-10, p. 513-522Article in journal (Refereed) Published
Abstract [en]

Purpose: Respiratory syncytial virus (RSV) is the most common cause of severe respiratory tract infection in infants. The aim was to identify host defence components in nasopharyngeal aspirate (NPA) from infants with RSV infection and to study the expression of the novel 25 kDa innate immunity protein SPLUNC1. less thanbrgreater than less thanbrgreater thanExperimental design: NPAs from infants were analyzed with 2-DE and MS in a pilot study. The levels of SPLUNC1 were analyzed with immunoblotting in 47 NPAs, admitted for RSV diagnosis. less thanbrgreater than less thanbrgreater thanResults: Totally, 35 proteins were identified in NPA, including several innate immunity proteins such as group X phospholipase A(2), different S100 proteins and SPLUNC1. In addition, a new truncated 15 kDa form of SPLUNC1 was identified that was detected in about 50% of the aspirates admitted for RSV diagnosis. RSV-positive boys had significantly less 25 kDa SPLUNC1 than RSV-negative boys while there were no significant differences among girls. less thanbrgreater than less thanbrgreater thanConclusions and clinical relevance: Several important innate immunity proteins were identified in NPA. Notably, a new truncated form of the newly suggested anti-bacterial protein SPLUNC1 was found. It is possible that a decrease in SPLUNC1 in the upper airways may increase the risk for severe pneumonia in boys.

Place, publisher, year, edition, pages
Wiley-VCH Verlag Berlin, 2011
Keywords
MS, Nasopharynx, PLUNC, Respiratory syncytial virus, Two-dimensional gel electrophoresis
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-72142 (URN)10.1002/prca.201100016 (DOI)000296418400005 ()
Note
Funding Agencies|The Research Council of South East Sweden|FORSS-36761- 8505|Available from: 2011-11-18 Created: 2011-11-18 Last updated: 2015-04-23
Karlsson, H., Lindbom, J., Ghafouri, B., Lindahl, M., Tagesson, C., Gustafsson, M. & Ljungman, A. G. (2011). Wear Particles from Studded Tires and Granite Pavement Induce Pro-inflammatory Alterations in Human Monocyte-Derived Macrophages: A Proteomic Study.. Chemical Research in Toxicology, 24, 45-53
Open this publication in new window or tab >>Wear Particles from Studded Tires and Granite Pavement Induce Pro-inflammatory Alterations in Human Monocyte-Derived Macrophages: A Proteomic Study.
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2011 (English)In: Chemical Research in Toxicology, ISSN 0893-228X, E-ISSN 1520-5010, Vol. 24, p. 45-53Article in journal (Refereed) Published
Abstract [en]

Airborne particulate matter is considered to be one of the environmental contributors to the mortality in cancer, respiratory, and cardiovascular diseases. For future preventive actions, it is of major concern to investigate the toxicity of defined groups of airborne particles and to clarify their pathways in biological tissues. To expand the knowledge beyond general inflammatory markers, this study examined the toxicoproteomic effects on human monocyte derived macrophages after exposure to wear particles generated from the interface of studded tires and a granite-containing pavement. As comparison, the effect of endotoxin was also investigated. The macrophage proteome was separated using two-dimensional gel electrophoresis. Detected proteins were quantified, and selected proteins were identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry. Among analyzed proteins, seven were significantly decreased and three were increased by exposure to wear particles as compared to unexposed control cells. Endotoxin exposure resulted in significant changes in the expression of six proteins: four decreased and two increased. For example, macrophage capping protein was significantly increased after wear particle exposure only, whereas calgizzarin and galectin-3 were increased by both wear particle and endotoxin exposure. Overall, proteins associated with inflammatory response were increased and proteins involved in cellular functions such as redox balance, anti-inflammatory response, and glycolysis were decreased. Investigating the effects of characterized wear particles on human macrophages with a toxicoproteomic approach has shown to be useful in the search for more detailed information about specific pathways and possible biological markers.

Place, publisher, year, edition, pages
American Chemical Society, 2011
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-64206 (URN)10.1021/tx100281f (DOI)000286130100006 ()21117676 (PubMedID)
Available from: 2011-01-14 Created: 2011-01-14 Last updated: 2017-12-11Bibliographically approved
Karlsson, H., Mortstedt, H., Lindqvist, H., Tagesson, C. & Lindahl, M. (2009). Protein profiling of low-density lipoprotein from obese subjects. PROTEOMICS CLINICAL APPLICATIONS, 3(6), 663-671
Open this publication in new window or tab >>Protein profiling of low-density lipoprotein from obese subjects
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2009 (English)In: PROTEOMICS CLINICAL APPLICATIONS, ISSN 1862-8346, Vol. 3, no 6, p. 663-671Article in journal (Refereed) Published
Abstract [en]

Although obesity and high levels of low-density lipoprotein (LDL) are well-known risk factors for cardiovascular disease, the precise role(s) of different LDL constituents in obesity has not been explored. In the present study, we compared the LDL proteome of healthy control adults (body mass index less than 25) and obese subjects (body mass index greater than 30). LDL was isolated by density-gradient ultracentrifugation and proteins were separated with 2-D PAGE, quantified, and identified by peptide mass fingerprinting using MALDI-TOF MS. A new LDL-associated protein was identified as transthyretin and found to be significantly more abundant in LDL from the obese subjects. In addition, LDL from the obese subjects contained relatively more alpha(1)-antitrypsin, apo J, apo C-II, than LDL from controls, and also more of an acidic isoform (pI/Mr; 5.2/23 100) of apo A-I. On the other hand, the relative amounts of apo A-IV and the major isoform of apo A-I (pI/Mr; 5.3/23 100) were significantly less in LDL from the obese subjects. Apo E was less and non-sialylated apo C-III more abundant in LDL from obese men than control men, while there were no such differences between LDL from obese and control women. These findings illustrate that obesity is not only associated with increased LDL-cholesterol levels but also with alterations in the LDL protein composition. The presence of transthyretin in LDL from obese subjects may reflect over-nutrition and affect the lipid metabolism in obesity.

Keywords
2-DE; Low-density lipoprotein; Obesity; Proteomics; Transthyretin
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-20158 (URN)10.1002/prca.200800138 (DOI)
Available from: 2009-09-01 Created: 2009-08-31 Last updated: 2010-01-09
Jayawardena, U., Tollemark, L., Tagesson, C. & Leanderson, P. (2009). Pyrogenic effect of respirable road dust particles. In: Lee Kenny, Fintan Hurley (Ed.), Inhaled Particles X. Paper presented at 10th International Symposium on Inhaled Particles,September 2008, Sheffield UK (pp. 012015). Institute of Physics Publishing (IOPP), 151
Open this publication in new window or tab >>Pyrogenic effect of respirable road dust particles
2009 (English)In: Inhaled Particles X / [ed] Lee Kenny, Fintan Hurley, Institute of Physics Publishing (IOPP), 2009, Vol. 151, p. 012015-Conference paper, Published paper (Refereed)
Abstract [en]

Because pyrogenic (fever-inducing) compounds on ambient particles may play an important role for particle toxicity, simple methods to measure pyrogens on particles are needed. Here we have used a modified in vitro pyrogen test (IPT) to study the release of interleukin 1β (IL-1β) in whole human blood exposed to respirable road-dust particles (RRDP). Road dusts were collected from the roadside at six different streets in three Swedish cities and particles with a diameter less than 10 μm (RRDP) were prepared by a water sedimentation procedure followed by lyophilisation. RRDP (200 μl of 1 - 106 ng/ml) were mixed with 50 μl whole blood and incubated at 37 °C overnight before IL-1β was analysed with chemiluminescence ELISA in 384-well plates. Endotoxin (lipopolysaccharide from Salmonella minnesota), zymosan B and Curdlan (P-1,3-glucan) were used as positive controls. All RRDP samples had a pyrogenic effect and the most active sample produced 1.6 times more IL-1β than the least active. This formation was of the same magnitude as in samples with 10 ng LPS/ml and was larger than that evoked by zymosan B and Curdlan (by mass basis). The method was sensitive enough to determine formation of IL-1β in mixtures with 10 ng RRDP/ml or 0.01 ng LPS/ml. The endotoxin inhibitor, polymyxin B (10 μg/ml), strongly reduced the RRDP-induced formation of IL-1β at 1μg RRDP/ml (around 80 % inhibition), but had only marginal or no effects at higher RRDP-concentrations (10 and 100 μg /ml). In summary, all RRDP tested had a clear pyrogen effect in this in vitro model. Endotoxin on the particles but also other factors contributed to the pyrogenic effect. As opposed to the limulus amebocyte lysate (LAL) assay (which measures endotoxin alone), IPT measures a broad range of pyrogens that may be present on particulate matter. The IPT method thus affords a simple, sensitive and quantitative determination of the total pyrogenic potential of ambient particles.

Place, publisher, year, edition, pages
Institute of Physics Publishing (IOPP), 2009
Series
Journal of Physics: Conference Series, ISSN 1742-6588 ; 151:1
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-18729 (URN)10.1088/1742-6596/151/1/012015 (DOI)000274607800117 ()
Conference
10th International Symposium on Inhaled Particles,September 2008, Sheffield UK
Available from: 2009-06-03 Created: 2009-06-03 Last updated: 2012-06-07
Ghafouri, B., Karlsson, H., Mörtstedt, H., Lewander, A., Tagesson, C. & Lindahl, M. (2007). Note: 2,5-Dihydroxybenzoic acid instead of α-cyano-4-hydroxycinnamic acid as matrix in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for analyses of in-gel digests of silver-stained proteins: in Analytical Biochemistry(ISSN 0003-2697), vol 371, issue 1, pp 121-123 [Letter to the editor]. Analytical Biochemistry, 371(1), 121-123
Open this publication in new window or tab >>Note: 2,5-Dihydroxybenzoic acid instead of α-cyano-4-hydroxycinnamic acid as matrix in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for analyses of in-gel digests of silver-stained proteins: in Analytical Biochemistry(ISSN 0003-2697), vol 371, issue 1, pp 121-123
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2007 (English)In: Analytical Biochemistry, ISSN 0003-2697, E-ISSN 1096-0309, Vol. 371, no 1, p. 121-123Article in journal, Letter (Other academic) Published
Abstract [en]

[No abstract available]

Place, publisher, year, edition, pages
Elsevier, 2007
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-39563 (URN)10.1016/j.ab.2007.07.002 (DOI)49770 (Local ID)49770 (Archive number)49770 (OAI)
Available from: 2009-10-10 Created: 2009-10-10 Last updated: 2017-12-13Bibliographically approved
Ghafouri, B., Karlsson, H., Lewander, A., Tagesson, C. & Lindahl, M. (2007). Peptide mass fingerprint data from silver stained proteins can be improved by using 2,5-dihydroxybenzoic acid instead of α-cyano-4-hydroxycinnamic acid as matrix in MALDI-TOF MS.
Open this publication in new window or tab >>Peptide mass fingerprint data from silver stained proteins can be improved by using 2,5-dihydroxybenzoic acid instead of α-cyano-4-hydroxycinnamic acid as matrix in MALDI-TOF MS
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2007 (English)Article in journal (Refereed) Submitted
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-14352 (URN)
Available from: 2007-03-15 Created: 2007-03-15
Hammar, M., Hultman, P. & Tagesson, C. (2007). Strålning, cancer och forskarutbildning. Läkartidningen, 104
Open this publication in new window or tab >>Strålning, cancer och forskarutbildning
2007 (Swedish)In: Läkartidningen, ISSN 0023-7205, E-ISSN 1652-7518, Vol. 104Article in journal (Other academic) Published
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-42050 (URN)59973 (Local ID)59973 (Archive number)59973 (OAI)
Available from: 2009-10-10 Created: 2009-10-10 Last updated: 2017-12-13
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