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Sundberg, Carina
Publications (10 of 15) Show all publications
Ziels, R. M., Svensson, B. H., Sundberg, C., Larsson, M., Karlsson, A. & Shakeri Yekta, S. (2018). Microbial rRNA gene expression and co-occurrence profiles associate with biokinetics and elemental composition in full-scale anaerobic digesters. Microbial Biotechnology, 11(4), 694-709
Open this publication in new window or tab >>Microbial rRNA gene expression and co-occurrence profiles associate with biokinetics and elemental composition in full-scale anaerobic digesters
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2018 (English)In: Microbial Biotechnology, ISSN 1751-7907, E-ISSN 1751-7915, Vol. 11, no 4, p. 694-709Article in journal (Refereed) Published
Abstract [en]

This study examined whether the abundance and expression of microbial 16S rRNA genes were associated with elemental concentrations and substrate conversion biokinetics in 20 full-scale anaerobic digesters, including seven municipal sewage sludge (SS) digesters and 13 industrial codigesters. SS digester contents had higher methane production rates from acetate, propionate and phenyl acetate compared to industrial codigesters. SS digesters and industrial codigesters were distinctly clustered based on their elemental concentrations, with higher concentrations of NH3-N, Cl, K and Na observed in codigesters. Amplicon sequencing of 16S rRNA genes and reverse-transcribed 16S rRNA revealed divergent grouping of microbial communities between mesophilic SS digesters, mesophilic codigesters and thermophilic digesters. Higher intradigester distances between Archaea 16S rRNA and rRNA gene profiles were observed in mesophilic codigesters, which also had the lowest acetate utilization biokinetics. Constrained ordination showed that microbial rRNA and rRNA gene profiles were significantly associated with maximum methane production rates from acetate, propionate, oleate and phenyl acetate, as well as concentrations of NH3-N, Fe, S, Mo and Ni. A co-occurrence network of rRNA gene expression confirmed the three main clusters of anaerobic digester communities based on active populations. Syntrophic and methanogenic taxa were highly represented within the subnetworks, indicating that obligate energy-sharing partnerships play critical roles in stabilizing the digester microbiome. Overall, these results provide new evidence showing that different feed substrates associate with different micronutrient compositions in anaerobic digesters, which in turn may influence microbial abundance, activity and function.

Place, publisher, year, edition, pages
Wiley-Blackwell Publishing Inc., 2018
National Category
Microbiology
Identifiers
urn:nbn:se:liu:diva-149709 (URN)10.1111/1751-7915.13264 (DOI)000435946800011 ()29633555 (PubMedID)2-s2.0-85045737255 (Scopus ID)
Note

Funding Agencies|Swedish Energy Agency; Biogas Research Centre at Linkoping University, Sweden; NSF [DGE-1256082]

Available from: 2018-07-24 Created: 2018-07-24 Last updated: 2018-08-14Bibliographically approved
Moestedt, J., Nordell, E., Shakeri Yekta, S., Lundgren, J., Marti, M., Sundberg, C., . . . Björn, A. (2016). Effects of trace element addition on process stability during anaerobic co-digestion of OFMSW and slaughterhouse waste. Waste Management, 47(Pt A), 11-20
Open this publication in new window or tab >>Effects of trace element addition on process stability during anaerobic co-digestion of OFMSW and slaughterhouse waste
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2016 (English)In: Waste Management, ISSN 0956-053X, E-ISSN 1879-2456, Vol. 47, no Pt A, p. 11-20Article in journal (Refereed) Published
Abstract [en]

This study used semi-continuous laboratory scale biogas reactors to simulate the effects of trace-element addition in different combinations, while degrading the organic fraction of municipal solid waste and slaughterhouse waste. The results show that the combined addition of Fe, Co and Ni was superior to the addition of only Fe, Fe and Co or Fe and Ni. However, the addition of only Fe resulted in a more stable process than the combined addition of Fe and Co, perhaps indicating a too efficient acidogenesis and/or homoacetogenesis in relation to a Ni-deprived methanogenic population. The results were observed in terms of higher biogas production (+9%), biogas production rates (+35%) and reduced VFA concentration for combined addition compared to only Fe and Ni. The higher stability was supported by observations of differences in viscosity, intraday WA-and biogas kinetics as well as by the 16S rRNA gene and 16S rRNA of the methanogens.(c) 2015 Elsevier Ltd. All rights reserved.

Place, publisher, year, edition, pages
PERGAMON-ELSEVIER SCIENCE LTD, 2016
Keywords
Trace elements; Anaerobic digestion; OFMSW; Methanogenic population; Viscosity
National Category
Renewable Bioenergy Research Environmental Sciences related to Agriculture and Land-use
Identifiers
urn:nbn:se:liu:diva-124642 (URN)10.1016/j.wasman.2015.03.007 (DOI)000367857900003 ()25827257 (PubMedID)
Note

Funding Agencies|Swedish Energy Agency; Linkoping University; Tekniska verken i Linkoping AB; Scandinavian Biogas Fuels AB; NSR AB; Kemira OYJ

Available from: 2016-02-08 Created: 2016-02-08 Last updated: 2018-01-10
Moestedt, J., Nordell, E., Lundgren, J., Genero Marti, M., Sundberg, C., Ejlertsson, J., . . . Björn, A. (2014). Effects of trace element addition on process stability during anaerobic co-digestion of OFMSW and slaughterhouse waste. In: : . Paper presented at Fifth International Symposium on Energy from Biomass and Waste, San Servolo, Venice, Italy, 17-20 November, 2014.
Open this publication in new window or tab >>Effects of trace element addition on process stability during anaerobic co-digestion of OFMSW and slaughterhouse waste
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2014 (English)Conference paper, Published paper (Refereed)
National Category
Natural Sciences
Identifiers
urn:nbn:se:liu:diva-114904 (URN)
Conference
Fifth International Symposium on Energy from Biomass and Waste, San Servolo, Venice, Italy, 17-20 November, 2014
Funder
Swedish Energy Agency
Available from: 2015-03-05 Created: 2015-03-05 Last updated: 2017-05-15
Bergfur, J. & Sundberg, C. (2014). Leaf-litter-associated fungi and bacteria along temporal and environmental gradients in boreal streams. Aquatic Microbial Ecology, 73(3), 225-234
Open this publication in new window or tab >>Leaf-litter-associated fungi and bacteria along temporal and environmental gradients in boreal streams
2014 (English)In: Aquatic Microbial Ecology, ISSN 0948-3055, E-ISSN 1616-1564, Vol. 73, no 3, p. 225-234Article in journal (Refereed) Published
Abstract [en]

Aquatic fungi and bacteria have long been recognized as key drivers in ecosystem processes such as leaf litter decomposition. We examined fungal and bacterial communities on decaying alder Alnus glutinosa (L.) Gaertner leaves along a gradient of increasing agricultural land use and associated nutrient enrichment in 9 boreal streams during 4 separate seasons (fall 2003, spring 2005, fall 2005, and spring 2006). Denaturing gradient gel electrophoresis (DGGE) and quantitative polymerase chain reactions (qPCR) showed that agricultural land use had significant effects on both bacterial and fungal communities, and on the ratios of fungi to total microbes associated with decomposing leaf litter. Furthermore, landscape factors and fluvial geomorphology appeared to influence the community composition of fungi and bacteria. Seasonal effects were found for fungal community structure only, indicating a higher temperature sensitivity of fungi compared to bacteria.

Place, publisher, year, edition, pages
Inter Research, 2014
Keywords
Sweden; Leaf litter; DGGE; qPCR; Aquatic fungi; Aquatic bacteria; Season; Land use
National Category
Earth and Related Environmental Sciences
Identifiers
urn:nbn:se:liu:diva-114265 (URN)10.3354/ame01721 (DOI)000347763200004 ()
Note

Funding Agencies|Langmannska kulturstiftelsen; European Commission (STAR project, 5th framework programme) [EVK1-CT-2001-00089]; European Commission (Euro-limpacs project, 6th framework programme) [GOCE-CT-2003-505540]

Available from: 2015-02-16 Created: 2015-02-16 Last updated: 2017-12-04
Robroek, B. J. M., Jasper Wubs, E., Generó, M. M., Zajac, K., Palsgaard Andersen, J., Andersson, A., . . . Verhoeyen, J. T. A. (2014). Microclimatological consequences for plant and microbial composition in Sphagnum-dominated peatlands. Boreal environment research, 19(3), 195-208
Open this publication in new window or tab >>Microclimatological consequences for plant and microbial composition in Sphagnum-dominated peatlands
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2014 (English)In: Boreal environment research, ISSN 1239-6095, E-ISSN 1797-2469, Vol. 19, no 3, p. 195-208Article in journal (Refereed) Published
Abstract [en]

In three Scandinavian peatlands we studied to what extent plant and microbial community compositions are governed by local-scale microhabitat, with a special interest in the effect of aspect (i.e. exposition of slopes). Despite differences in solar irradiance between the south- and north-facing slopes, maximum temperature was elevated in the south-facing slopes at the most northern site only. Pore-water nutrient concentrations were not affected by aspect, yet dissolved organic carbon concentrations were higher in the south-facing microhabitats. This was likely caused by higher vascular plant biomass. Plant and microbial community composition clearly differed among sites. In all three sites, microhabitat (i.e. prevailing water-table depth) affected the plant and microbial community compositions. Aspect, however, did not affect community composition, even though microclimate significantly differed between the south- and the north-facing aspects at the northernmost site. Our results highlight the complex link between plant community composition, microbial community and environmental conditions, which deserves much more attention than currently in order to fully understand the effects of climate change on peatland ecosystem function.

Place, publisher, year, edition, pages
Helsinki, Finland: Finnish Environment Institute, 2014
National Category
Earth and Related Environmental Sciences
Identifiers
urn:nbn:se:liu:diva-109258 (URN)000338392100004 ()2-s2.0-84902329593 (Scopus ID)
Available from: 2014-08-12 Created: 2014-08-11 Last updated: 2017-12-05Bibliographically approved
Sundberg, C., Al-Soud, W. A., Larsson, M., Alm, E., Shakeri Yekta, S., Svensson, B., . . . Karlsson, A. (2013). 454 pyrosequencing analyses of bacterial and archaeal richness in 21 full-scale biogas digesters. FEMS Microbiology Ecology, 85(3), 612-626
Open this publication in new window or tab >>454 pyrosequencing analyses of bacterial and archaeal richness in 21 full-scale biogas digesters
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2013 (English)In: FEMS Microbiology Ecology, ISSN 0168-6496, E-ISSN 1574-6941, Vol. 85, no 3, p. 612-626Article in journal (Refereed) Published
Abstract [en]

The microbial community of 21 full-scale biogas reactors was examined using 454 pyrosequencing of 16S rRNA gene sequences. These reactors included seven (six mesophilic and one thermophilic) digesting sewage sludge (SS) and 14 (ten mesophilic and four thermophilic) codigesting (CD) various combinations of wastes from slaughterhouses, restaurants, households, etc. The pyrosequencing generated more than 160 000 sequences representing 11 phyla, 23 classes, and 95 genera of Bacteria and Archaea. The bacterial community was always both more abundant and more diverse than the archaeal community. At the phylum level, the foremost populations in the SS reactors included Actinobacteria, Proteobacteria, Chloroflexi, Spirochetes, and Euryarchaeota, while Firmicutes was the most prevalent in the CD reactors. The main bacterial class in all reactors was Clostridia. Acetoclastic methanogens were detected in the SS, but not in the CD reactors. Their absence suggests that methane formation from acetate takes place mainly via syntrophic acetate oxidation in the CD reactors. A principal component analysis of the communities at genus level revealed three clusters: SS reactors, mesophilic CD reactors (including one thermophilic CD and one SS), and thermophilic CD reactors. Thus, the microbial composition was mainly governed by the substrate differences and the process temperature.

Place, publisher, year, edition, pages
Wiley-Blackwell, 2013
Keywords
microbial community, anaerobic digestion, sewage sludge, codigestion
National Category
Social Sciences
Identifiers
urn:nbn:se:liu:diva-97233 (URN)10.1111/1574-6941.12148 (DOI)000323200400017 ()
Note

Funding Agencies|Swedish Energy Agency||

Available from: 2013-09-06 Created: 2013-09-05 Last updated: 2017-12-06
Gustavsson, J., Shakeri Yekta, S., Sundberg, C., Karlsson, A., Ejlertsson, J., Skyllberg, U. & Svensson, B. (2013). Bioavailability of cobalt and nickel during anaerobic digestion of sulfur-rich stillage for biogas formation. Applied Energy, 112, 473-477
Open this publication in new window or tab >>Bioavailability of cobalt and nickel during anaerobic digestion of sulfur-rich stillage for biogas formation
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2013 (English)In: Applied Energy, ISSN 0306-2619, E-ISSN 1872-9118, Vol. 112, p. 473-477Article in journal (Refereed) Published
Abstract [en]

Addition of Co and Ni often improves the production of biogas during digestion of organic matter, i.e. increasing CH4-production, process stability and substrate utilization which often opens for higher organic loading rates (OLRs). The effect of Co and Ni addition was evaluated by measuring methane production, volatile solids reduction, pH and concentration of volatile fatty acids (VFAs). A series of six lab-scale semi-continuously fed biogas tank reactors were used for this purpose. The chemical forms and potential bioavailability of Co and Ni were examined by sequential extraction, acid volatile sulfide extraction (AVS) and simultaneously extracted metals. Furthermore, the sulfur speciation in solid phase was examined by sulfur X-ray absorption near edge structure spectroscopy. The effect of Co and Ni deficiency on the microbial community composition was analyzed using quantitative polymerase chain reaction and 454-pyrosequencing. The results showed that amendment with Co and Ni was necessary to maintain biogas process stability and resulted in increased CH4-production and substrate utilization efficiency. 10-20% of the total Co concentration was in dissolved form and should be regarded as easily accessible by the microorganisms. In contrast, Ni was entirely associated with organic matter/sulfides (mainly AVS) and regarded as very difficult to take up. Still Ni had stimulatory effects suggesting mechanisms such as dissolution of NiS to be involved in the regulation of Ni availability for the microorganisms. The microbial community structure varied in relation to the occurrence of Ni and Co. The acetate-utilizing Methanosarcinales dominated during stable process performance, i.e. when both Co and Ni were supplied, while hydrogenotrophic Methanomicrobiales increased together with VFA concentrations under Co or Ni deficiency. The increase was more pronounced at Co limitation. This study demonstrates that there are good possibilities to improve the performance of biogas processes digesting sulfur-rich substrates by supplementation of Co and Ni.

Place, publisher, year, edition, pages
Elsevier, 2013
Keywords
Biogas production; Trace element; Bioavailability; Speciation; Cobalt; Nickel
National Category
Social Sciences
Identifiers
urn:nbn:se:liu:diva-104128 (URN)10.1016/j.apenergy.2013.02.009 (DOI)000329377800048 ()
Available from: 2014-02-07 Created: 2014-02-07 Last updated: 2017-12-06
Karlsson, A., Einarsson, P., Schnurer, A., Sundberg, C., Ejlertsson, J. & Svensson, B. (2012). Impact of trace element addition on degradation efficiency of volatile fatty acids, oleic acid and phenyl acetate and on microbial populations in a biogas digester. Journal of Bioscience and Bioengineering, 114(4), 446-452
Open this publication in new window or tab >>Impact of trace element addition on degradation efficiency of volatile fatty acids, oleic acid and phenyl acetate and on microbial populations in a biogas digester
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2012 (English)In: Journal of Bioscience and Bioengineering, ISSN 1389-1723, E-ISSN 1347-4421, Vol. 114, no 4, p. 446-452Article in journal (Refereed) Published
Abstract [en]

The effect of trace element addition on anaerobic digestion of food industry- and household waste was studied using two semi-continuous lab-scale reactors, one (R30+) was supplied with Fe, Co and Ni, while the other (R30) acted as a control. Tracer analysis illustrated that methane production from acetate proceeded through syntrophic acetate oxidation (SAO) in both digesters. The effect of the trace elements was also evaluated in batch assays to determine the capacity of the microorganisms of the two digesters to degrade acetate, phenyl acetate, oleic acid or propionate, butyrate and valerate provided as a cocktail. The trace elements addition improved the performance of the process giving higher methane yields during start-up and early operation and lower levels of mainly acetate and propionate in the R30+ reactor. The batch assay showed that material from R30+ gave effects on methane production from all substrates tested. Phenyl acetate was observed to inhibit methane formation in the R30 but not in the R30+ assay. A real-time PCR analysis targeting methanogens on the order level as well as three SAO bacteria showed an increase in Methanosarcinales in the R30+ reactor over time, even though SAO continuously was the dominating pathway for methane production. Possibly, this increase explains the low VFA-levels and higher degradation rates observed in the R30+ batch incubations. These results show that the added trace elements affected the ability of the microflora to degrade VFAs as well as oleic acid and phenyl acetate in a community, where acetate utilization is dominated by SAO.

Place, publisher, year, edition, pages
Elsevier, 2012
Keywords
Trace element; Volatile fatty acids; Long chain fatty acids; Syntrophic acetate oxidation; Real-time PCR
National Category
Social Sciences
Identifiers
urn:nbn:se:liu:diva-87229 (URN)10.1016/j.jbiosc.2012.05.010 (DOI)000311595400015 ()
Available from: 2013-01-17 Created: 2013-01-14 Last updated: 2017-12-06
Sundberg, C., Sundblad-Tonderski, K. & Lindgren, P.-E. (2011). Ammonia oxidation and the corresponding bacterial communities in two overland flow areas treating landfill leachate or wastewater. In: Tommy S W Wong (Ed.), Overland Flow and Surface Runoff: (pp. 346). Nova Science Publishers, Inc.
Open this publication in new window or tab >>Ammonia oxidation and the corresponding bacterial communities in two overland flow areas treating landfill leachate or wastewater
2011 (English)In: Overland Flow and Surface Runoff / [ed] Tommy S W Wong, Nova Science Publishers, Inc., 2011, p. 346-Chapter in book (Refereed)
Abstract [en]

A high diversity of ammonium oxidising bacteria (AOB) has been observed in overland flow areas (OFA) treating ammonia-rich landfill leachate. The current section aimed to explore if treatment OFAs in general supports more diverse AOB communities than conventional treatment systems, or if it is a result of effluent composition. The potential ammonium oxidation and the AOB community composition were studied during three seasons in an OFA where one part received wastewater and the other landfill leachate. The AOB communities were investigated using group-specific PCR primers targeting the 16S rRNA gene, and analysed by DGGE and nucleotide sequencing. The potential ammonia oxidation, studied by short-time slurry incubation, was higher in the landfill OFA than in the wastewater area and highest in the litter layer. Higher activity correlated with the appearance of Nitrosomonas sp. belonging to cluster 7. Both overland flow areas supported a more diverse AOB community than in common wastewater treatment plants. Fifteen different AOB sequences were detected, but only three were observed in both OFAs, pointing to the impact of the effluent quality and/or the hydraulic load. The wastewater OFA, which received a higher load of effluents with 5-10 times lower ammonia concentrations, was dominated by AOB populations that are usually found in less favourable conditions.

Place, publisher, year, edition, pages
Nova Science Publishers, Inc., 2011
Keywords
Runoff -- Mathematical models, Streamflow -- Mathematical models, Frictional resistance (Hydrodynamics), ´Water Pollution, Nitrifying bacteria, SCIENCE / Earth Sciences / Hydrology, SCIENCE / Earth Sciences / Hydrology
National Category
Natural Sciences
Identifiers
urn:nbn:se:liu:diva-17922 (URN)978-1-61122-868-7 (ISBN)
Available from: 2009-04-24 Created: 2009-04-24 Last updated: 2018-03-13Bibliographically approved
Hellman, J., Ek, A., Sundberg, C., Johansson, M., Svensson, B. & Karlsson, M. (2010). Mechanisms of increased methane production through re-circulation of magnetic biomass carriers in an experimental continuously stirred tank reactor. Paper presented at 12th World Congress on Anaerobic Digestion (AD12). Oct 31st - Nov 4th, 2010. Guadalajara, Mexico. IWA Publishing
Open this publication in new window or tab >>Mechanisms of increased methane production through re-circulation of magnetic biomass carriers in an experimental continuously stirred tank reactor
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2010 (English)Conference paper, Published paper (Refereed)
Abstract [en]

Magnetite particles were used in a semi-continuous process as magnetic biomass carriers to separate and re-introduce microorganisms in a CSTR reactor. In comparison to a control reactor the methane content during the semi-continuous process was elevated when magnetite particles were used. The difference was most apparent during the fermentative step directly after feeding and upon direct spiking with volatile fatty acids. Total DNA quantification of the separated magnetite particles revealed high association of microorganisms. Furthermore, quantitative real-time PCR analysis of the associated microbial consortia indicated that the hydrogenotrophic Methanobacteriales was overrepresented at the particle surface. Thus, the increased methane production could be coupled to both the crowding and shorter interspecies distances between the groups involved in anaerobic digestion, as well as a preferential adsorption of hydrogenotrophs. By bringing the hydrogenotrophs closer to the primary fermentative bacteria and increasing their relative number the produced hydrogen during acidogenesis is more effectively utilized and more carbon dioxide is converted to methane. Furthermore, by the same cause, the rate of acetogenesis increased as the hydrogenotrophs more effectively could consume the hydrogen produced and thereby keep the hydrogen partial pressure low.

Place, publisher, year, edition, pages
IWA Publishing, 2010
Keywords
anaerobic digestion; bio carrier; biofilm; magnetite; syntrophy
National Category
Natural Sciences
Identifiers
urn:nbn:se:liu:diva-74090 (URN)
Conference
12th World Congress on Anaerobic Digestion (AD12). Oct 31st - Nov 4th, 2010. Guadalajara, Mexico
Available from: 2012-01-19 Created: 2012-01-18 Last updated: 2012-10-01Bibliographically approved
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