Open this publication in new window or tab >>Scottish Meningococcus and Pneumococcus Reference Laboratory, Stobhill Hospital and Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow.
Scottish Meningococcus and Pneumococcus Reference Laboratory, Stobhill Hospital, Glasgow.
Meningococcal Reference Unit, Health Protection Agency, Manchester Royal Infirmary, Manchester, United Kingdom.
Institute for Hygiene and Microbiology, National Reference Center for Meningococci, University of Würzburg, Germany.
Meningococcal Reference Unit, Health Protection Agency, Manchester Royal Infirmary, Manchester, United Kingdom.
Meningococcal Reference Unit, Health Protection Agency, Manchester Royal Infirmary, Manchester, United Kingdom.
National Reference Centre for Meningococci, Austrian Agency for Health and Food Safety, Graz, Austria.
National Reference Laboratory for Meningococcal Infections, National Institute of Public Health, Prague, Czech Republic.
National Meningococcal Reference Laboratory, NIPH, Athens, Greece.
WHO Collaborating Center for Reference and Research on Meningococci, Division of Infectious Disease Control, Norwegian Institute of Public Health, Oslo, Norway.
National Reference Laboratory for Meningococcal Infections, National Institute of Public Health, Prague, Czech Republic.
Meningococcal Reference Unit, Health Protection Agency, Manchester Royal Infirmary, Manchester, United Kingdom.
National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, University Hospital, Örebro, Sweden.
Irish Meningococcal and Meningitis Reference Laboratory, The Children's University Hospital, Dublin, Ireland.
National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, University Hospital, Örebro, Sweden.
Centre Muraz, Bobo Dioulasso, Burkina Faso.
National Meningococcal Reference Laboratory, NIPH, Athens, Greece.
Institute for Hygiene and Microbiology, National Reference Center for Meningococci, University of Würzburg, Germany.
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2005 (English)In: Journal of Clinical Microbiology, ISSN 0095-1137, E-ISSN 1098-660X, Vol. 43, no 1, p. 144-149Article in journal (Refereed) Published
Abstract [en]
Twenty clinical samples (18 cerebrospinal fluid samples and 2 articular fluid samples) were sent to 11 meningococcus reference centers located in 11 different countries. Ten of these laboratories are participating in the EU-MenNet program (a European Union-funded program) and are members of the European Monitoring Group on Meningococci. The remaining laboratory was located in Burkina Faso. Neisseria meningitidis was sought by detecting several meningococcus-specific genes (crgA, ctrA, 16S rRNA, and porA). The PCR-based nonculture method for the detection of N. meningitidis gave similar results between participants with a mean sensitivity and specificity of 89.7 and 92.7%, respectively. Most of the laboratories also performed genogrouping assays (siaD and mynB/sacC). The performance of genogrouping was more variable between laboratories, with a mean sensitivity of 72.7%. Genogroup B gave the best correlation between participants, as all laboratories routinely perform this PCR. The results for genogroups A and W135 were less similar between the eight participating laboratories that performed these PCRs.
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-33470 (URN)10.1128/JCM.43.1.144-149.2005 (DOI)19493 (Local ID)19493 (Archive number)19493 (OAI)
2009-10-092009-10-092017-12-13Bibliographically approved