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Hedin, Linnea
Publications (2 of 2) Show all publications
Lloyd Spetz, A., Pearce, R., Hedin, L., Khranovskyy, V., Söderlind, F., Käll, P.-O., . . . Uvdal, K. (2009). New transducer material concepts for biosensors and surface functionalization. In: Ulrich Schmid, Carles Cané, Herbert Shea (Ed.), Smart Sensors, Actuators,and MEMS IV: . Paper presented at Smart Sensors, Actuators and MEMS IV, Dresden, Germany, 4–6 May 2009 (pp. 736206). Bellingham, WA United States: SPIE - International Society for Optical Engineering, 7362
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2009 (English)In: Smart Sensors, Actuators,and MEMS IV / [ed] Ulrich Schmid, Carles Cané, Herbert Shea, Bellingham, WA United States: SPIE - International Society for Optical Engineering, 2009, Vol. 7362, p. 736206-Conference paper, Published paper (Refereed)
Abstract [en]

Wide bandgap materials like SiC, ZnO, AlN form a strong platform as transducers for biosensors realized as e.g. ISFET (ion selective field effect transistor) devices or resonators. We have taken two main steps towards a multifunctional biosensor transducer. First we have successfully functionalized ZnO and SiC surfaces with e.g. APTES. For example ZnO is interesting since it may be functionalized with biomolecules without any oxidation of the surface and several sensing principles are possible. Second, ISFET devises with a porous metal gate as a semi-reference electrode are being developed. Nitric oxide, NO, is a gas which participates in the metabolism. Resistivity changes in Ga doped ZnO was demonstrated as promising for NO sensing also in humid atmosphere, in order to simulate breath.

Place, publisher, year, edition, pages
Bellingham, WA United States: SPIE - International Society for Optical Engineering, 2009
Series
Proceedings of SPIE - International Society for Optical Engineering, ISSN 0277-786X ; Vol. 7362
National Category
Natural Sciences
Identifiers
urn:nbn:se:liu:diva-21212 (URN)10.1117/12.821662 (DOI)000299794400006 ()9780819476364 (ISBN)
Conference
Smart Sensors, Actuators and MEMS IV, Dresden, Germany, 4–6 May 2009
Note

Original Publication: Anita Lloyd Spetz, Ruth Pearce, Linnea Hedin, Volodymyr Khranovskyy, Fredrik Söderlind, Per-Olov Käll, Rositsa Yakimova and Kajsa Uvdal, New transducer material concepts for biosensors and surface functionalization, Proceedings of SPIE - The International Society for Optical Engineering, ed Ulrich Schmid , 7362, 736206, 2009. http://dx.doi.org/10.1117/12.821662 Copyright 2009 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

Available from: 2009-09-30 Created: 2009-09-30 Last updated: 2015-05-29Bibliographically approved
Nyström, S., Kågedal, K., Jonsson, M., Hedin, L., Svensson, S. & Hammarström, P.Properties of defined recombinant oligomeric forms of Aβ1‐42.
Open this publication in new window or tab >>Properties of defined recombinant oligomeric forms of Aβ1‐42
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(English)Manuscript (preprint) (Other academic)
Abstract [en]

Oligomers of Aβ1-42 have been identified in human Alzheimer´s disease (AD) patients and in mouse models of AD. These species have attracted intense interest as possible neurological pathogens in AD. In our hands, expression of recombinant human Aβ1-42 in Escherichia coli followed by purification in the presence of cupric ions (CuCl2) afforded recovery of high quantities (>5 mg/L of culture) of well defined trimeric, hexameric, nonameric and dodecameric Aβ1-42. Strong denaturing conditions such as 6 M GuHCI, 8 M urea or boiling in 6.5 M urea supplemented with 2.5 % SDS all failed to separate the oligomers into smaller building blocks implicating that the oligomers are composed of covalently cross-linked Aβ1-42 monomers. Purification in the absence of cupric ions resulted in monomeric Aβ1-42. The Aβ1-42 oligomers were toxic and induced apoptosis when administered to neuroblastoma cells in culture. The described method producing oligomeric Aβ1-42 from a recombinant expression system paves the way for mechanistic studies, structural analysis, drug screening and opens up for vaccine development.

National Category
Natural Sciences
Identifiers
urn:nbn:se:liu:diva-53175 (URN)
Available from: 2010-01-18 Created: 2010-01-18 Last updated: 2019-11-08
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