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Nayeri, Fariba
Publications (10 of 36) Show all publications
Ljunggren, S., Bengtsson, T., Karlsson, H., Starkhammar Johansson, C., Palm, E., Nayeri, F., . . . Lönn, J. (2019). Modified lipoproteins in periodontitis: a link to cardiovascular disease?. Bioscience Reports, 39(3), Article ID BSR20181665.
Open this publication in new window or tab >>Modified lipoproteins in periodontitis: a link to cardiovascular disease?
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2019 (English)In: Bioscience Reports, ISSN 0144-8463, E-ISSN 1573-4935, Vol. 39, no 3, article id BSR20181665Article in journal (Refereed) Published
Abstract [en]

There is a strong association between periodontal disease and atherosclerotic cardiovascular disorders. A key event in the development of atherosclerosis is accumulation of modified lipoproteins within the arterial wall. We hypothesise that patients with periodontitis have an altered lipoprotein profile towards an atherogenic form. Therefore, the present study aims at identifying modifications of plasma lipoproteins in periodontitis. Lipoproteins from ten female patients with periodontitis and gender- and age-matched healthy controls were isolated by density-gradient ultracentrifugation. Proteins were separated by 2D gel-electrophoresis and identified by map-matching or by nano-LC followed by MS. Apolipoprotein (Apo) A-I (ApoA-I) methionine oxidation, Oxyblot, total antioxidant capacity and a multiplex of 71 inflammation-related plasma proteins were assessed. Reduced levels of apoJ, phospholipid transfer protein, apoF, complement C3, paraoxonase 3 and increased levels of alpha-1-antichymotrypsin, apoA-II, apoC-III were found in high-density lipoprotein (HDL) from the patients. In low-density lipoprotein (LDL)/very LDL (VLDL), the levels of apoL-1 and platelet-activating factor acetylhydrolase (PAF-AH) as well as apo-B fragments were increased. Methionine oxidation of apoA-I was increased in HDL and showed a relationship with periodontal parameters. alpha-1 antitrypsin and alpha-2-HS glycoprotein were oxidised in LDL/VLDL and antioxidant capacity was increased in the patient group. A total of 17 inflammation-related proteins were important for group separation with the highest discriminating proteins identified as IL-21, Fractalkine, IL-17F, IL-7, IL-1RA and IL-2. Patients with periodontitis have an altered plasma lipoprotein profile, defined by altered protein levels as well as post-translational and other structural modifications towards an atherogenic form, which supports a role of modified plasma lipoproteins as central in the link between periodontal and cardiovascular disease (CVD).

Place, publisher, year, edition, pages
Portland Press, 2019
National Category
Cardiac and Cardiovascular Systems
Identifiers
urn:nbn:se:liu:diva-157252 (URN)10.1042/BSR20181665 (DOI)000465453700016 ()30842338 (PubMedID)2-s2.0-85063936955 (Scopus ID)
Note

Funding Agencies|Swedish Knowledge Foundation [Dnr20150037]; Foundation Langmanska Kulturfonden; Magnus Bergwalls Foundation

Available from: 2019-06-04 Created: 2019-06-04 Last updated: 2019-06-10Bibliographically approved
Ramezani, A., Nägga, K., Hansson, O., Lönn, J., Sjöwall, J., Katoozian, F., . . . Nayeri, F. (2015). Hepatocyte growth factor in cerebrospinal fluid differentiates community-acquired or nosocomial septic meningitis from other causes of pleocytosis. Fluids and Barriers of the CNS, 12(1)
Open this publication in new window or tab >>Hepatocyte growth factor in cerebrospinal fluid differentiates community-acquired or nosocomial septic meningitis from other causes of pleocytosis
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2015 (English)In: Fluids and Barriers of the CNS, ISSN 2045-8118, E-ISSN 2045-8118, Vol. 12, no 1Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: Due to anatomical restrictions, the inflammatory response to intracerebral bacterial infections exposes swollen brain tissues to pressure and ischemia, resulting in life-threatening damage. Rapid diagnosis and immediate empirical antibiotic therapy is highly important. However, diagnosing meningitis in patients after neurosurgery is complicated, due to brain tissue damage and changes in cerebrospinal fluid (CSF) caused by surgery. Hepatocyte growth factor (HGF) is a local, acute-phase protein with healing properties. Previous studies on community-acquired septic meningitis reported high levels of intrathecally produced HGF. The present study focused on nosocomial meningitis in assessing the levels of HGF in the CSF.

METHODS: HGF concentrations (ELISA) and HGF binding to receptors; c-Met receptor and heparan sulfate proteoglycan were determined in CSF samples (surface plasmon resonance). CSF samples from patients with community-acquired or nosocomial meningitis (217 samples from 135 patients) were compared to those from controls without signs of cerebral nervous system involvement (N = 36) and patients with Alzheimer's disease (N = 20).

RESULTS: Compared to samples from patients that had undergone neurosurgery and had other infectious diseases, CSF samples from patients with nosocomial meningitis had significantly higher HGF concentrations (p < 0.001) and binding affinity to c-Met (p < 0.001) and HSPG (p = 0.043) receptors. The sensitivity and specificity to identify nosocomial septic meningitis were 69.7 and 93.4 %, respectively. The HGF concentration and binding affinity to HGF receptors were significantly higher in CSF from patients with community-acquired septic meningitis compared to patients with aseptic (viral and subacute) meningitis as well as controls (p < 0.001). The sensitivity and specificity to identify community-acquired septic meningitis were 95.4 and 95.7 %, respectively.

DISCUSSION: In febrile nosocomial infections that occurred post neurosurgery, HGF assessment could substantially improve the differentiation of meningitis from other infections and therefore might be a tool for rapid diagnosis, limiting injuries and guiding antibiotic therapy.

National Category
Infectious Medicine Pharmaceutical Sciences
Identifiers
urn:nbn:se:liu:diva-122242 (URN)10.1186/s12987-015-0020-z (DOI)26408034 (PubMedID)
Available from: 2015-10-26 Created: 2015-10-26 Last updated: 2019-02-11
Williams, G. S., Naiene, J., Gayflor, J., Malibiche, T., Zoogley, B., Frank, W. G. & Nayeri, F. (2015). Twenty-one days of isolation: A prospective observational cohort study of an Ebola-exposed hot zone community in Liberia. Journal of Infection, 71(2), 150-7
Open this publication in new window or tab >>Twenty-one days of isolation: A prospective observational cohort study of an Ebola-exposed hot zone community in Liberia
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2015 (English)In: Journal of Infection, ISSN 0163-4453, E-ISSN 1532-2742, Vol. 71, no 2, p. 150-7Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: As West Africa continues to suffer from a deadly Ebola epidemic, the national health sectors struggle to minimize the damages and stop the spread of disease.

METHODS: A cohort of inhabitants of a small village and an Ebola hot zone in Sinoe County of Liberia was followed on a day-by-day basis to search for new cases and to minimize the spread of Ebola to the other community members or to other regions. Technical, clinical, and humanistic aspects of the response are discussed in this report.

RESULTS: Of the 22 confirmed Ebola cases in Sinoe County since the beginning of outbreak (June 16, 2014), 7 cases were inhabitants of Polay Town, a small village 5.5 miles east of Greenville, the Sinoe County capital. After the last wave of outbreak at the beginning of December, enhanced response activity provided essential coordination and mobilized the resources to stop the epidemic. Despite unprotected contacts in crowded houses, no new cases were detected among the contact families, or in the surrounding houses or communities.

CONCLUSIONS: Strong national mobilization in a decentralized but harmonized system at the community level has been of great value in controlling the epidemic in Liberia. The major interventions include epidemiological surveillance, public information dissemination, effective communication, case management, and infection control.

National Category
Infectious Medicine
Identifiers
urn:nbn:se:liu:diva-120614 (URN)10.1016/j.jinf.2015.05.003 (DOI)000365137700002 ()25982026 (PubMedID)
Available from: 2015-08-18 Created: 2015-08-18 Last updated: 2017-12-04
Sorour, A. E., Lönn, J., Nakka, S. S., Nayeri, T. & Nayeri, F. (2014). Evaluation of hepatocyte growth factor as a local acute phase response marker in the bowel: The clinical impact of a rapid diagnostic test for immediate identification of acute bowel inflammation. Cytokine, 71(1), 8-15
Open this publication in new window or tab >>Evaluation of hepatocyte growth factor as a local acute phase response marker in the bowel: The clinical impact of a rapid diagnostic test for immediate identification of acute bowel inflammation
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2014 (English)In: Cytokine, ISSN 1043-4666, E-ISSN 1096-0023, Vol. 71, no 1, p. 8-15Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: There are no rapid tests that can distinguish contagious gastroenteritis, which requires isolation at its onset, from exacerbation of chronic inflammatory bowel disease (IBD) or bowel engagement in the course of systemic inflammatory response syndrome (SIRS). Hepatocyte growth factor (HGF) is an acute phase cytokine that is produced at the site of injury. It has high affinity to sulfated glycan, and this binding affinity is lost during chronic inflammation. The fecal pH strongly impacts the prognosis for severe bowel disease. We developed a strip test to evaluate HGF as a local acute phase response marker in the bowel. This test assessed the binding affinity of HGF to sulfated glycans in fecal samples and determined fecal pH as an indicator of illness severity.

METHODS: Fresh feces from patients with diarrhea (n=513) were collected and tested blindly, and information about patient illness course and outcome was collected. Patients were classified based on the focus of inflammation and the cause of the symptoms. Objectively verified diagnoses of infectious gastroenteritis (n=131) and IBD onset/exacerbation and bowel cancer (n=44) were used to estimate the performance of the test strip. ELISA was performed on 101 freeze-thawed feces samples to determine the fecal HGF levels.

RESULTS: The test rapidly distinguished infectious gastroenteritis from non-infectious inflammatory causes of diarrhea (sensitivity, 87.96%; specificity, 90.9%; positive predictive value, 96.6%; negative predictive value, 71.4%; accuracy, 89.1%). Fecal pH (p<0.0001) and mortality within 28days of sampling (p<0.04) was higher in patients with sepsis/SIRS and diarrhea. The concentration of HGF was higher in strip test-positive stool samples (p<0.01).

CONCLUSIONS: HGF is a good local acute phase response marker of acute bowel inflammation. Test-strip determination of the binding affinity of fecal HGF to sulfated glycan was a rapid, equipment-free way to assess patients with diarrhea and to guide the diagnostic and therapeutic approaches on admission.

Place, publisher, year, edition, pages
Elsevier, 2014
Keywords
HGF; Inflammatory bowel disease; Local acute phase response; Rapid test; Transmittable diarrhea
National Category
Clinical Medicine Basic Medicine
Identifiers
urn:nbn:se:liu:diva-110994 (URN)10.1016/j.cyto.2014.07.255 (DOI)000347584600002 ()25174881 (PubMedID)
Available from: 2014-10-02 Created: 2014-10-02 Last updated: 2018-01-11Bibliographically approved
Abednazari, H., Brudin, L., Almroth, G., Nilsson, I. & Nayeri, F. (2014). Hepatocyte growth factor is a reliable marker for efficient anti-bacterial therapy within the first day of treatment. Advances in Bioscience and Biotechnology, 5(10), 823-830
Open this publication in new window or tab >>Hepatocyte growth factor is a reliable marker for efficient anti-bacterial therapy within the first day of treatment
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2014 (English)In: Advances in Bioscience and Biotechnology, ISSN 2156-8456, E-ISSN 2156-8502, Vol. 5, no 10, p. 823-830Article in journal (Refereed) Published
Abstract [en]

Rapid diagnosis and choice of appropriate antibiotic treatment might be life-saving in serious infectious diseases. Still the available markers that can evaluate and monitor the diagnosis and treatment are few. Hepatocyte growth factor (HGF) has been studied as a potent regenerative factor produced and released during injuries such as infectious diseases. Monitoring of HGF levels might predict therapy results better than C-reactive protein (CRP) within the first day of treatment in pneumonia. For further investigation of previous observations we aimed to study HGF as a first-day marker in over-representing infectious diseases in comparison to procalcitonin (PCT), CRP and body temperature. Fifty-one patients with community acquired infectious diseases were included consequently at admittance and the serum samples were collected before and within 18 - 24 hours of treatment. HGF levels decreased significantly in case of efficient antibiotic therapy and HGF was shown to be better than PCT, CRP and body temperature to evaluate treatment. In patients with pneumonia, monitoring of HGF was most reasonable. HGF might be used as a therapeutic marker within the first day of empiric antibiotic treatment during infection.

Place, publisher, year, edition, pages
Scientific Research Publishing, 2014
Keywords
Hepatocyte Growth Factor, C-Reactive Protein, Procalcitonin, Temperature, Antibacterial Therapy
National Category
Clinical Medicine
Identifiers
urn:nbn:se:liu:diva-110998 (URN)10.4236/abb.2014.510096 (DOI)
Available from: 2014-10-02 Created: 2014-10-02 Last updated: 2017-12-05Bibliographically approved
Lönn, J., Starkhammar Johansson, C., Nakka, S., Palm, E., Bengtsson, T., Nayeri, F. & Ravald, N. (2014). High Concentration but Low Activity of Hepatocyte Growth Factor in Periodontitis. Journal of Periodontology, 85(1), 113-122
Open this publication in new window or tab >>High Concentration but Low Activity of Hepatocyte Growth Factor in Periodontitis
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2014 (English)In: Journal of Periodontology, ISSN 0022-3492, E-ISSN 1943-3670, Vol. 85, no 1, p. 113-122Article in journal (Refereed) Published
Abstract [en]

Background: High levels of hepatocyte growth factor (HGF), a healing factor with regenerative and cytoprotective effects, are associated with inflammatory diseases, including periodontitis. HGF biologic activity requires binding to its receptors, the proto-oncogene c-Met and heparan sulfate proteoglycan (HSPG). This study investigates HGF expression and its relationship to subgingival microbiota in medically healthy individuals with and without periodontitis.

Methods: Saliva, gingival crevicular fluid (GCF), and blood samples from 30 patients with severe periodontitis and 30 healthy controls were analyzed for HGF concentration using enzyme-linked immunosorbent assay and binding affinity for HSPG and c-Met using surface plasmon resonance. The regenerative effects of saliva from three patients and controls were analyzed in an in vitro model of cell injury. Subgingival plaques were analyzed for the presence of 18 bacterial species.

Results: Patients with periodontitis showed higher HGF concentrations in saliva, GCF, and serum (P <0.001); however, the binding affinities for HSPG and c-Met were reduced in GCF and saliva (P <0.002). In contrast to the controls, saliva from patients showed no significant regenerative effect over time on gingival epithelial cells. Compared with controls, patients had a higher prevalence of periodontally related bacteria.

Conclusions: Higher circulatory HGF levels indicate a systemic effect of periodontitis. However, the HGF biologic activity at local inflammation sites was reduced, and this effect was associated with the amount of periodontal bacteria. Loss of function of healing factors may be an important mechanism in degenerative processes in periodontally susceptible individuals.

Place, publisher, year, edition, pages
American Academy of Periodontology, 2014
Keywords
Gingival crevicular fluid, hepatocyte growth factor, microbiology, periodontal diseases, saliva, serum
National Category
Clinical Medicine Dentistry Cell and Molecular Biology
Identifiers
urn:nbn:se:liu:diva-103611 (URN)10.1902/jop.2013.130003 (DOI)000331139400016 ()
Available from: 2014-01-21 Created: 2014-01-21 Last updated: 2018-01-11Bibliographically approved
Sowdamini Nakka, S., Johansson, J., Shahzad, F., Hanning, A. & Nayeri, F. (2013). A methachromatic-based experimental model for identification of bowel as the focus of an acute inflammation. Open Journal of Gastroenterology, 3(1), 42-48
Open this publication in new window or tab >>A methachromatic-based experimental model for identification of bowel as the focus of an acute inflammation
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2013 (English)In: Open Journal of Gastroenterology, ISSN 2163-9450, E-ISSN 2163-9469, Vol. 3, no 1, p. 42-48Article in journal (Refereed) Published
Abstract [en]

Diarrhea is the most common symptom of acute inflammation in gastrointestinal tract and the patients are isolated in order to inhibit transmission and to conduct investigations. Yet there is no standard test to distinguish gastrointestinal infection from more generalized diseases at admittance which might cause delay in therapy. Hepatocyte growth factor (HGF) is produced upon injury by mesenchymal cells. On the contrary to chronic inflammation, HGF produced in the course of acute inflammation is biologically active and shows binding affinity to heparan sulphate proteoglycan (HSPG) and dextran sulphate (DS). Based on this phenomenon, an agarose gel containing DS was prepared and immobilized on loops to investigate the feces samples for the presence or absence of growth factors such as HGF with affinity to DS. The study is conducted as a clinical evaluation of an experimental model to distinguish acute infectious gastroenteritis from other causes of diarrhea. 656 fecal samples gathered consequently from patients seeking for bowel disturbances and healthy were tested by the test and the medical reports were investigated. Upon interaction with DS, methylene blue changes color to pink. This phenomenon was inhibited by HGF and converted by addition of anti-HGF antibodies to the samples. The test distinguished acute infectious gastroenteritis with high sensitivity and specificity (96% and 92% respectively) from other causes of diarrhea. We introduce a metachromatic experimental model that might distinguish acute inflammation in alimentary tract from other causes of diarrhea. This model might be used in developing rapid diagnostic tests.

Place, publisher, year, edition, pages
Scientific Research Publishing, 2013
Keywords
Gastroenteritis; Hepatocyte Growth Factor; HSPG; Metachromasy; Rapid Test
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-98306 (URN)10.4236/ojgas.2013.31007 (DOI)
Available from: 2013-10-08 Created: 2013-10-08 Last updated: 2017-12-06Bibliographically approved
Almroth, G., Lonn, J., Uhlin, F., Nayeri, F., Brudin, L., Andersson, B. & Hahn-Zoric, M. (2013). Fibroblast Growth Factor 23, Hepatocyte Growth Factor, Interleukin-6, High-Sensitivity C-Reactive Protein and Soluble Urokinase Plasminogen Activator Receptor. Inflammation Markers in Chronic Haemodialysis Patients?. Scandinavian Journal of Immunology, 78(3), 285-290
Open this publication in new window or tab >>Fibroblast Growth Factor 23, Hepatocyte Growth Factor, Interleukin-6, High-Sensitivity C-Reactive Protein and Soluble Urokinase Plasminogen Activator Receptor. Inflammation Markers in Chronic Haemodialysis Patients?
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2013 (English)In: Scandinavian Journal of Immunology, ISSN 0300-9475, E-ISSN 1365-3083, Vol. 78, no 3, p. 285-290Article in journal (Refereed) Published
Abstract [en]

Sera from 84 haemodialysis (HD) patients and 68 healthy blood donors were analysed with commercially available ELISA techniques for fibroblast growth factor 23 (FGF-23), hepatocyte growth factor (HGF), interleukin-6 (Il-6), high-sensitivity C-reactive protein (hs-CRP) and soluble urokinase plasminogen activator receptor (suPAR), to find a possible correlation of FGF-23 and HGF with the earlier recognized inflammatory markers Il-6 and hs-CRP or suPAR. All patients studied had significantly elevated levels of FGF-23, HGF, hs-CRP and suPAR as compared to the controls. Il-6 and hs-CRP correlated for patients (R=0.6) as well as for patients and controls altogether. Ln (natural logarithm) of HGF correlated weakly with Ln Il-6 and Ln CRP (R 0.28-0.37). Ln FGF-23 correlated only with Ln HGF (r=-0.25) in controls. Ln HGF correlated with ln suPAR (r=0.6) in both patients and controls. Although elevated as compared to controls, we found no correlation of FGF-23 with the recognized inflammatory markers Il-6, hs-CRP, nor HGF or the new marker suPAR in HD patients. Ln HGF correlated with Ln Il-6, Ln CRP and Ln suPAR. Although probably involved in vessel disease, FGF-23 and HGF may play other roles than acting in inflammatory vessel disease in HD patients. Further studies are necessary to evaluate the role of these immunological markers in chronic haemodialysis patients with atherosclerosis.

Place, publisher, year, edition, pages
Wiley-Blackwell, 2013
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-97434 (URN)10.1111/sji.12082 (DOI)000323377100008 ()
Note

Funding Agencies|Medical Research Council of Southeast Sweden (FORSS)||

Available from: 2013-09-12 Created: 2013-09-12 Last updated: 2017-12-06
Almroth, G., Lönn, J., Uhlin, F., Nayeri, F., Brudin, L., Andersson, B. & Hahn-Zoric, M. (2013). Tillväxtfaktorer och inflammationsmarkörer vid kronisk njursvikt. In: Njurmedicinskt vårmöte Jönköping 12-14 maj 2013: . Paper presented at Njurmedicinskt vårmöte, Jönköping, 12-14 maj 2013.
Open this publication in new window or tab >>Tillväxtfaktorer och inflammationsmarkörer vid kronisk njursvikt
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2013 (Swedish)In: Njurmedicinskt vårmöte Jönköping 12-14 maj 2013, 2013Conference paper, Published paper (Refereed)
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-102331 (URN)
Conference
Njurmedicinskt vårmöte, Jönköping, 12-14 maj 2013
Available from: 2013-12-05 Created: 2013-12-05 Last updated: 2014-01-15
Lönn, J., Almroth, G., Brudin, L. & Nayeri, F. (2012). An antithrombin III product containing biologically active hepatocyte growth factor may be beneficial in depp ulcer infections. Cytokine, 60(2), 478-486
Open this publication in new window or tab >>An antithrombin III product containing biologically active hepatocyte growth factor may be beneficial in depp ulcer infections
2012 (English)In: Cytokine, ISSN 1043-4666, E-ISSN 1096-0023, Vol. 60, no 2, p. 478-486Article in journal (Refereed) Published
Abstract [en]

BACKGROUND:

Widely studied for the past 20 years, hepatocyte growth factor (HGF) has been identified as a regenerative marker and an important factor in the development and healing of injuries. Antithrombin III (AT III) is a protein in the blood stream with anti-thrombotic and anti-inflammatory properties and has been used as an adjuvant treatment along with antibiotics in severe sepsis.

OBJECTIVE:

To study the content and properties of HGF in plasma-derived AT III products, and the regenerative effect in severe deep ulcer infections.

METHODS:

Commercial AT III products were analyzed for the presence and biological activity of HGF. One AT III product containing biologically active HGF was used to treat 18 cases of critical, deep ulcer infections scheduled for major invasive intervention. The patients were followed up for 6-60 months.

RESULTS:

The AT III products contained HGF with different biological activity. No adverse reactions were observed after local administration of AT III during the study or follow-up period. In 16 of 18 cases no surgical intervention was needed within the first 6 month of inclusion.

CONCLUSION:

AT III products containing biologically active HGF may contribute to regeneration and healing in severe deep ulcer infections which do not respond adequately to different combinations of antibiotics alone.

Place, publisher, year, edition, pages
Elsevier, 2012
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-86279 (URN)10.1016/j.cyto.2012.05.023 (DOI)000310494300024 ()
Available from: 2012-12-12 Created: 2012-12-12 Last updated: 2017-12-07
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