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Serrander, Lena
Publications (10 of 13) Show all publications
Sundbom, P., Hübbert, L. & Serrander, L. (2017). Progressive multifocal leukoencephalopathy after heart transplantation: 4 years of clinically stable infection on low-dose immunosuppressive therapy. Oxford Medical Case Reports, 2017(2), 15-17
Open this publication in new window or tab >>Progressive multifocal leukoencephalopathy after heart transplantation: 4 years of clinically stable infection on low-dose immunosuppressive therapy
2017 (English)In: Oxford Medical Case Reports, E-ISSN 2053-8855, Vol. 2017, no 2, p. 15-17Article in journal (Refereed) Published
Abstract [en]

Progressive multifocal leukoencephalopathy (PML), caused by reactivation of JC-virus is a relatively rare complication seen in patients with compromised immune system. There are no evidence-based treatment available and prognosis is poor. Withdrawal of immunosuppressant can result in further neurological deterioration and for patients with solid organ transplantations, fatal graft rejection. We report a 52-year-old women that presented with seizures within 1 month after heart transplantation. Initial diagnosis was vascular disease. After clinical deterioration 10 months after transplantation, further examinations led to the diagnosis. Minimizing tacrolimus, to a concentration of 2 ng/ml, and extensive physical therapy has improved the physical capacity of the patient. The patient has now been clinically stable for 4 years and extended survival for 5 years. This case adds to the limited adult cases of PML within the population of heart transplant recipients and the need for increased awareness to minimize diagnosis delay.

Place, publisher, year, edition, pages
Oxford Academic, 2017
National Category
Surgery Infectious Medicine
Identifiers
urn:nbn:se:liu:diva-145288 (URN)10.1093/omcr/omx003 (DOI)28473916 (PubMedID)
Available from: 2018-02-23 Created: 2018-02-23 Last updated: 2018-07-30Bibliographically approved
Crisci, E., Ellegård, R., Nyström, S., Rondahl, E., Serrander, L., Bergström, T., . . . Larsson, M. (2016). Complement opsonization promotes HSV-2 infection of human dendritic cells. Journal of Virology, 90(10), 4939-4950
Open this publication in new window or tab >>Complement opsonization promotes HSV-2 infection of human dendritic cells
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2016 (English)In: Journal of Virology, ISSN 0022-538X, E-ISSN 1098-5514, Vol. 90, no 10, p. 4939-4950Article in journal (Refereed) Published
Abstract [en]

Herpes virus type 2 (HSV2) is one of the most common sexually transmitted infections globally with a very high prevalence in many countries. During HSV2 infection viral particles become coated with complement proteins and antibodies, both existent in the genital fluids, which could influence the activation of the immune responses. In genital mucosa, the primary target cells for HSV2 infection are epithelial cells, but resident immune cells such as dendritic cells (DCs) are also infected. The DCs are the activators of the ensuing immune responses directed against HSV2, and the aim of this study was to examine the effects opsonization of HSV2, either with complement alone or with complement and antibodies, had on the infection of immature DCs and their ability to mount inflammatory and antiviral responses. Complement opsonization of HSV2 enhanced both the direct infection of immature DCs and their production of new infectious viral particles. The enhanced infection required activation of the complement cascade and functional complement receptor 3. Furthermore, HSV2 infection of DCs required endocytosis of viral particles and their delivery into an acid endosomal compartment. The presence of complement in combination with HSV1 or HSV2 specific antibodies more or less abolished the HSV2 infection of DCs.Our results clearly demonstrate the importance of studying HSV2 infection under conditions that ensue in vivo, i.e. when the virions are covered in complement fragments and complement fragments and antibodies, as this will shape the infection and the subsequent immune response and needs to be further elucidated.

IMPORTANCE: During HSV2 infection viral particles should become coated with complement proteins and antibodies, both existent in the genital fluids, which could influence the activation of the immune responses. The dendritic cells are the activators of the immune responses directed against HSV2, and the aim of this study was to examine the effects of complement alone or complement and antibodies, on the HSV2 infection of dendritic cells and their ability to mount inflammatory and antiviral responses.Our results demonstrate that the presence of antibodies and complement in the genital environment can influence HSV2 infection under in vitro conditions that reflect the in vivo situation. We believe that our findings are highly relevant for the understanding of HSV2 pathogenesis.

Place, publisher, year, edition, pages
American society of microbiology, 2016
Keywords
HSV2 infection, dendritic cells, complement, antibodies
National Category
Infectious Medicine Microbiology in the medical area
Identifiers
urn:nbn:se:liu:diva-126480 (URN)10.1128/JVI.00224-16 (DOI)000375126100009 ()26937039 (PubMedID)
Note

Funding agencies: Swedish Research Council [AI52731]; Swedish Physicians Against AIDS Research Foundation; Swedish International Development Cooperation Agency; SIDA SARC; VINNMER for Vinnova; Linkoping University Hospital Research Fund; Swedish Society of Medicine; Swedis

Available from: 2016-03-29 Created: 2016-03-29 Last updated: 2018-03-23
Åkerlund, A., Sundqvist, M., Hanberger, H., Åhrén, C., Serrander, L. & Giske, C. G. (2015). Svarstiderna kan kortas vid mikrobiologisk diagnostik av sepsis: Bättre öppettider på laboratorier och aktiv rådgivning ger snabbare terapi. Läkartidningen, 112(7), Article ID C73S.
Open this publication in new window or tab >>Svarstiderna kan kortas vid mikrobiologisk diagnostik av sepsis: Bättre öppettider på laboratorier och aktiv rådgivning ger snabbare terapi
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2015 (Swedish)In: Läkartidningen, ISSN 0023-7205, E-ISSN 1652-7518, Vol. 112, no 7, article id C73SArticle in journal (Refereed) Published
Abstract [sv]

Snabbt insatt adekvat antibiotikabehandling är livräddande vid allvarliga bakteriella infektioner. 

Snabb mikrobiologisk diagnostik krävs i och med ökande antibiotikaresistens och kommer att ge medicinska vinster.

En enkät till landets mikrobiologiska laboratorier visar på stora skillnader avseende tillgänglighet, snabbhet och kommunikation med svarsmottagande enhet vad gäller positiva blododlingar.

För snabbare svar krävs att mikrobiologiska laboratorier erbjuder mer generösa öppettider, effektivare transportsystem och patientnära blododlingsinkubatorer samt tidig och aktiv rådgivning till behandlande läkare.

n/a

National Category
Infectious Medicine
Identifiers
urn:nbn:se:liu:diva-126420 (URN)
Available from: 2016-03-24 Created: 2016-03-24 Last updated: 2017-11-30
Larsson, M. C., Karlsson, E., Woksepp, H., Frolander, K., Mårtensson, A., Rashed, F., . . . Serrander, L. (2014). Rapid identification of pneumococci, enterococci, beta-haemolytic streptococci and S. aureus from positive blood cultures enabling early reports. BMC Infectious Diseases, 14(146)
Open this publication in new window or tab >>Rapid identification of pneumococci, enterococci, beta-haemolytic streptococci and S. aureus from positive blood cultures enabling early reports
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2014 (English)In: BMC Infectious Diseases, ISSN 1471-2334, E-ISSN 1471-2334, Vol. 14, no 146Article in journal (Refereed) Published
Abstract [en]

BACKGROUND:

The aim of this study was to evaluate diagnostic tests in order to introduce a diagnostic strategy to identify the most common gram-positive bacteria (pneumococci, enterococci, β-haemolytic streptococci and S. aureus) found in blood cultures within 6 hours after signalling growth.

METHODS:

The tube coagulase test was optimized and several latex agglutination tests were compared and evaluated before a validation period of 11 months was performed on consecutive positive blood culture patient samples from Kalmar County Hospital, Sweden.

RESULTS:

During the validation period 150 (91%) of a total of 166 gram-positive cocci (119 in clusters, 45 in chains or pairs and 2 undefined morphology) were correctly identified as S. aureus, CoNS, Pneumococci, Enterococci or group A streptococci (GAS), group B streptococci (GBS), group G streptococci (GGS) within 6 hours with a minimal increase in work-load and costs. The remaining samples (9%) were correctly identified during the next day. No samples were incorrectly grouped with this diagnostic strategy and no patient came to risk by early reporting.

CONCLUSION:

A simple strategy gives reliable and cost-effective reporting of >90% of the most common gram-positive cocci within 6 hours after a blood cultures become positive. The high specificity of the tests used makes preliminary reports reliable. The reports can be used to indicate the focus of infection and not the least, support faster administration of proper antimicrobial treatment for patients with serious bacterial infections.

Place, publisher, year, edition, pages
BioMed Central, 2014
Keywords
Sepsis; Microbiology; Rapid diagnostics; Bacteraemia; Agglutinations; Gram-positive
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-106290 (URN)10.1186/1471-2334-14-146 (DOI)000333600200002 ()24645982 (PubMedID)
Available from: 2014-05-06 Created: 2014-05-05 Last updated: 2019-02-11Bibliographically approved
Rondahl, E., Gruber, M., Joelsson, S., Sundqvist, M., Åkerlind, B., Cardell, K., . . . Serrander, L. (2014). The risk of HCV RNA contamination in serology screening instruments with a fixed needle for sample transfer.. Journal of Clinical Virology, 60(2), 172-173
Open this publication in new window or tab >>The risk of HCV RNA contamination in serology screening instruments with a fixed needle for sample transfer.
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2014 (English)In: Journal of Clinical Virology, ISSN 1386-6532, E-ISSN 1873-5967, Vol. 60, no 2, p. 172-173Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: Hepatitis C diagnostics involve antibody screening and confirmation of current infection by detection of HCV RNA positivity. In screening instruments with fixed pipetting needle, there is a risk of sample carry-over contamination.

OBJECTIVES: The aim of this study was to evaluate the risk of such contamination in a proposed clinical setting.

STUDY DESIGN: In the present study, known HCV RNA positive (n=149) and negative (n=149) samples were analysed by anti-HCV Abbott in an Architect instrument in an alternating fashion in order to test for contamination.

RESULTS: In subsequent retesting of the previously HCV RNA-negative samples, six samples (4%) were positive by the Cobas Taqman assay with a maximum level of 33IU/mL. The results show that there is a risk for transfer of HCV in the Architect instrument but they also show that the levels of HCV RNA observed are low.

CONCLUSIONS: We conclude that complementary HCV RNA testing on samples identified as anti-HCV positive by screening can be recommended because the complementary results are reliable in the majority of cases when either HCV RNA is negative or HCV RNA is positive with a level >1000IU/mL. In a minority of cases, with low HCV RNA after anti-HCV antibody screening, cross-contamination should be suspected and a new sample requested for HCV RNA testing. This strategy would reduce the need for obtaining a new sample from the vast majority of patients with a newly discovered HCV antibody positivity.

National Category
Microbiology in the medical area
Identifiers
urn:nbn:se:liu:diva-107297 (URN)10.1016/j.jcv.2014.03.011 (DOI)000335738000016 ()24735614 (PubMedID)
Available from: 2014-06-10 Created: 2014-06-10 Last updated: 2018-01-11
Nguyen, M. V., Lardy, B., Rousset, F., Hazane-Puch, F., Zhang, L., Trocme, C., . . . Morel, F. (2013). Quinone compounds regulate the level of ROS production by the NADPH oxidase Nox4. Biochemical Pharmacology, 85(11), 1644-1654
Open this publication in new window or tab >>Quinone compounds regulate the level of ROS production by the NADPH oxidase Nox4
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2013 (English)In: Biochemical Pharmacology, ISSN 0006-2952, E-ISSN 1356-1839, Vol. 85, no 11, p. 1644-1654Article in journal (Refereed) Published
Abstract [en]

NADPH oxidase Nox4 is expressed in a wide range of tissues and plays a role in cellular signaling by providing reactive oxygen species (ROS) as intracellular messengers. Nox4 oxidase activity is thought to be constitutive and regulated at the transcriptional level; however, we challenge this point of view and suggest that specific quinone derivatives could modulate this activity. In fact, we demonstrated a significant stimulation of Nox4 activity by 4 quinone derivatives (AA-861, tBuBHQ tBuBQ and duroquinone) observed in 3 different cellular models, HEK293E, T-REx (TM), and chondrocyte cell lines. Our results indicate that the effect is specific toward Nox4 versus Nox2. Furthermore, we showed that NAD(P)H:quinone oxidoreductase (NQO1) may participate in this stimulation. Interestingly, Nox4 activity is also stimulated by reducing agents that possibly act by reducing the disulfide bridge (Cys226, Cys270) located in the extracellular E-loop of Nox4. Such model of Nox4 activity regulation could provide new insight into the understanding of the molecular mechanism of the electron transfer through the enzyme, i.e., its potential redox regulation, and could also define new therapeutic targets in diseases in which quinones and Nox4 are implicated.

Place, publisher, year, edition, pages
Elsevier, 2013
Keywords
NADPH oxidase Nox4, NAD(P)H:quinone oxidoreductase NQO1, Quinones, Redox regulation of Nox, Reactive oxygen species (ROS)
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-95506 (URN)10.1016/j.bcp.2013.03.023 (DOI)000319491900010 ()
Note

Funding Agencies|Ministere de lEnseignement superieur de la recherche et la technologie, Paris, France||CNRS Institute||Association pour la Recherche contre le Cancer (ARC), Paris, France||Region Rhone-Alpes||programme ARCUS, France/Chine||CGD research Trust, UK||Groupement des Entreprises Francaises de la Lutte contre le Cancer, delegation de Grenoble||UFR de Medecine, Universite Joseph Fourier, Grenoble||Direction Regionale de la Recherche Clinique, Center Hospitalier Universitaire, Grenoble||

Available from: 2013-07-05 Created: 2013-07-05 Last updated: 2017-12-06Bibliographically approved
Jonsson, N., Wahlström, K., Svensson, L., Serrander, L. & Lindberg, A. M. (2012). Aichi virus infection in elderly people in Sweden. Archives of Virology, 157(7), 1365-1369
Open this publication in new window or tab >>Aichi virus infection in elderly people in Sweden
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2012 (English)In: Archives of Virology, ISSN 0304-8608, E-ISSN 1432-8798, Vol. 157, no 7, p. 1365-1369Article in journal (Refereed) Published
Abstract [en]

Aichi virus (AiV), genus Kobuvirus, family Picornaviridae, is associated with gastroenteritis in humans. Previous studies have shown high seroprevalence but low incidence (0.9-4.1%) in clinical samples. We report here the first detection of AiV in Sweden. Two hundred twenty-one specimens from hospitalized patients with diarrhea, who were negative for other enteric viruses, were included in the study. AiV were detected in three specimens, all from elderly patients. Phylogenetic analysis revealed that the three Swedish isolates belonged to genotype A and were genetically closest to European and Asian strains of AiV.

Place, publisher, year, edition, pages
Springer Verlag (Germany), 2012
National Category
Engineering and Technology
Identifiers
urn:nbn:se:liu:diva-79683 (URN)10.1007/s00705-012-1296-9 (DOI)000305795300017 ()
Available from: 2012-08-14 Created: 2012-08-13 Last updated: 2017-12-07
Sundén, B., Larsson, M., Falkeborn, T., Paues, J., Forsum, U., Lindh, M., . . . Serrander, L. (2011). Real-time PCR detection of Human Herpesvirus 1-5 in patients lacking clinical signs of a viral CNS infection. BMC Infectious Diseases, 11(220)
Open this publication in new window or tab >>Real-time PCR detection of Human Herpesvirus 1-5 in patients lacking clinical signs of a viral CNS infection
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2011 (English)In: BMC Infectious Diseases, ISSN 1471-2334, E-ISSN 1471-2334, Vol. 11, no 220Article in journal (Refereed) Published
Abstract [en]

BACKGROUND:

Infections of the central nervous system (CNS) with herpes- or enterovirus can be self-limiting and benign, but occasionally result in severe and fatal disease. The polymerase chain reaction (PCR) has revolutionized the diagnostics of viral pathogens, and by multiple displacement amplification (MDA) prior to real-time PCR the sensitivity might be further enhanced. The aim of this study was to investigate if herpes- or enterovirus can be detected in cerebrospinal fluid (CSF) from patients without symptoms.

METHODS:

Cerebrospinal fluid (CSF) samples from 373 patients lacking typical symptoms of viral CNS infection were analysed by real-time PCR targeting herpesviruses or enteroviruses with or without prior MDA.

RESULTS:

In total, virus was detected in 17 patients (4%). Epstein-Barr virus (EBV) was most commonly detected, in general from patients with other conditions (e.g. infections, cerebral hemorrhage). MDA satisfactorily amplified viral DNA in the absence of human nucleic acids, but showed poor amplification capacity for viral DNA in CSF samples, and did not increase the sensitivity for herpes virus-detection with our methodology.

CONCLUSIONS:

Viral pathogens are rarely detected in CSF from patients without signs of CNS infection, supporting the view that real-time PCR is a highly specific method to detect symptomatic CNS-infection caused by these viruses. However, EBV may be subclinically reactivated due to other pathological conditions in the CNS.

Place, publisher, year, edition, pages
BioMed Central, 2011
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-71558 (URN)10.1186/1471-2334-11-220 (DOI)000295288300001 ()21849074 (PubMedID)
Note

Funding Agencies|Linkoping University|ALF: LIO-17791 |

Available from: 2011-10-21 Created: 2011-10-21 Last updated: 2018-03-26
Lindmark, M., Karlsson, A., Serrander, L., Francois, P., Lew, D., Rasmusson, B., . . . Nüße, O. (2002). Synaptotagmin II could confer Ca2+ sensitivity to phagocytosis in human neutrophils. Biochimica et Biophysica Acta. Molecular Cell Research, 1590(1-3), 159-166
Open this publication in new window or tab >>Synaptotagmin II could confer Ca2+ sensitivity to phagocytosis in human neutrophils
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2002 (English)In: Biochimica et Biophysica Acta. Molecular Cell Research, ISSN 0167-4889, E-ISSN 1879-2596, Vol. 1590, no 1-3, p. 159-166Article in journal (Refereed) Published
Abstract [en]

Phagolysosome fusion and granule exocytosis in neutrophils are calcium-dependent processes. The calcium requirements vary between granule types, suggesting the presence of different calcium sensors. The synaptotagmins, a family of calcium-binding proteins, previously shown to participate in vesicle fusion and vesicle recycling in excitable cells, are putative calcium-sensors of exocytosis in excitable cells. In this study, we show that synaptotagmin II is present in human neutrophils and may participate in phagocytic and in exocytotic processes. In protein extracts from human neutrophils, we identified synaptotagmin II by Western blot as an 80 kDa protein. Subcellular fractionation revealed that synaptotagmin II was associated with the specific granules. In fMLP-stimulated cells, synaptotagmin II translocated to the plasma membrane. This correlated with the upregulation of complement receptor 3 (CR 3), reflecting the translocation of specific granules to the cell surface. Synaptotagmin II also translocated to the phagosome after complement-mediated phagocytosis in the presence of calcium. LAMP-1 translocated in parallel but probably was located to another subcellular compartment than synaptotagmin II. Under calcium-reduced conditions, neither synaptotagmin II nor LAMP-1 translocated to the phagosome. We therefore suggest a role for synaptotagmin II as calcium-sensor during phagocytosis and secretion in neutrophils.

National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-26522 (URN)10.1016/S0167-4889(02)00209-4 (DOI)11081 (Local ID)11081 (Archive number)11081 (OAI)
Available from: 2009-10-08 Created: 2009-10-08 Last updated: 2017-12-13
Larsson, J., Serrander, L., Stendahl, O. & Lundqvist-Gustafsson, H. (2000). Involvement of the ß2-integrin CD18 in apoptosis signal transduction in human neutrophils. Inflammation Research, 49(9), 452-459
Open this publication in new window or tab >>Involvement of the ß2-integrin CD18 in apoptosis signal transduction in human neutrophils
2000 (English)In: Inflammation Research, ISSN 1023-3830, E-ISSN 1420-908X, Vol. 49, no 9, p. 452-459Article in journal (Refereed) Published
Abstract [en]

Objective and design: To examine the hypothesis that an accelerated rate of neutrophil apoptosis occurs following β2-integrin activation, and further investigate the signal transduction pathways involved.

Material: Human polymorphonuclear neutrophils.

Treatment: Neutrophils were challenged with pansorbins coated with antibodies towards the β2-integrin subunit CD18 in a proportion of 1:100 with or without the inhibitors diphenylene iodonium (10 M), cytochalasin B (5 μg/ml), genistein (10 nM), herbimycin A (10 M) and Z-VAD-FMK (10 μM).

Methods: Measurement of phosphatidylserine exposure and DNA fragmentation in flow cytometry and assessment of H2O2-production through spectrofluorometry. The results were analysed using Mann Whitney U test and Kruskal Wallis.

Results: Pansorbins coated with antibodies to CD18 induce apoptosis in neutrophils (p < 0.01), and activate the production of reactive oxygen species (p < 0.01). Pre-treatment with the inhibitors have no effect on anti-CD18 induced apoptosis.

Conclusion: Anti-CD18 pansorbins induce apoptosis in neutrophils through an alternative pathway not involving reactive oxygen species and independent of tyrosine phosphorylation, cytoskeletal reorganisation and caspases.

National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-26104 (URN)10.1007/s000110050616 (DOI)10563 (Local ID)10563 (Archive number)10563 (OAI)
Available from: 2009-10-08 Created: 2009-10-08 Last updated: 2017-12-13Bibliographically approved
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