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Gati, Istvan
Publications (8 of 8) Show all publications
Danielsson, O., Lindvall, B., Gati, I. & Ernerudh, J. (2013). Classification and Diagnostic Investigation in Inflammatory Myopathies: A Study of 99 Patients. Journal of Rheumatology, 40(7), 1173-1182
Open this publication in new window or tab >>Classification and Diagnostic Investigation in Inflammatory Myopathies: A Study of 99 Patients
2013 (English)In: Journal of Rheumatology, ISSN 0315-162X, E-ISSN 1499-2752, Vol. 40, no 7, p. 1173-1182Article in journal (Refereed) Published
Abstract [en]

Objective. Insights into the pathogenesis of inflammatory myopathies have led to new diagnostic methods. The aims of our study were (1) to evaluate the consequences of using the classification of Amato/European Neuromuscular Centre Workshop (ENMC), compared to that of Bohan and Peter; and (2) to evaluate any diagnostic benefit in using an extended pathological investigation. less thanbrgreater than less thanbrgreater thanMethods. From a consecutive retrospective database, we evaluated 99 patients for classification. Patients with inclusion body myositis (IBM) were classified according to Griggs, et al. In addition to routine stainings and immunohistochemistry, a multilevel serial sectioning procedure was performed on paraffin-embedded material, to identify scarce pathological findings. less thanbrgreater than less thanbrgreater thanResults. Classification according to Bohan and Peter could be performed for 83 of the 99 patients, whereas only 60 patients met the Amato/ENMC criteria, the latter resulting in the following diagnostic groups: IBM (n = 18), nonspecific myositis (n = 14), polymyositis (n = 12), dermatomyositis (n = 10), dermatomyositis sine dermatitis (n = 5), and immune-mediated necrotizing myopathy (n = 1). Most of the Amato/ENMC diagnostic groups harbored patients from several of the Bohan and Peter groups, which included a substantial group lacking proximal muscle weakness. The serial sectioning procedure was essential for classification of 9 patients (15%), and led to a more specific diagnosis for 13 patients (22%) according to Amato/ENMC. less thanbrgreater than less thanbrgreater thanConclusion. The classification of Amato/ENMC was more restrictive, forming groups based on clinical criteria and specified myopathological findings, which clearly differed from the groups of the Bohan and Peter classification. An extended pathological investigation increased the diagnostic yield of a muscle biopsy and highlights the quantity and specificity of certain pathological findings.

Place, publisher, year, edition, pages
Journal of Rheumatology, 2013
Keywords
INFLAMMATORY MYOPATHIES, IDIOPATHIC INFLAMMATORY MYOPATHIES, POLYMYOSITIS, DERMATOMYOSITIS, INCLUSION BODY MYOSITIS
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-96992 (URN)10.3899/jrheum.120804 (DOI)000321993800023 ()
Note

Funding Agencies|University Hospital Linkoping||County Council of Ostergotland||

Available from: 2013-09-02 Created: 2013-09-02 Last updated: 2017-12-06
Dezsi, L., Danielsson, O., Gati, I., Timea Varga, E. & Vecsei, L. (2013). Inclusion body myositis - a rarely recognized disorder. Ideggyogyaszati Szemle - Clinical Neuroscience, 66(3-4), 89-101
Open this publication in new window or tab >>Inclusion body myositis - a rarely recognized disorder
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2013 (Hungarian)In: Ideggyogyaszati Szemle - Clinical Neuroscience, ISSN 0019-1442, Vol. 66, no 3-4, p. 89-101Article, review/survey (Refereed) Published
Abstract [en]

Inclusion body myositis is the most common disabling inflammatory myopathy in the elderly. It is more frequent in men and after the age of 50 years. Inflammatory and degenerative features coexist. There is a T-cell mediated autoimmunity driven by in situ clonally expanded cytotoxic CD8-positive T-cells invading non-necrotic muscle fibres expressing MHC-I antigen. The hallmarks of degeneration are the deposition of protein aggregates and the formation of vesicles. The course of the disease is slow and the diagnosis is usually set after several years. The muscle weakness and wasting is assymetric, affecting predominantly distal muscles of the upper extremity and proximal muscles of the legs. The signs and clinical course can be characteristic, but the diagnosis is established by muscle biopsy. less thanbrgreater than less thanbrgreater thanThere is currently no evidence based effective treatment for sIBM. Prednisone, azathioprine, methotrexate, cyclosporine and IFN-beta failed. Oxandrolon did not improve symptoms. Treatment with intravenous immunglobuline (IVIG) induced in some patients a transient improvement of swallowing and of muscle strenght, but the overall study results were negative. less thanbrgreater than less thanbrgreater thanA T-cell depleting monoclonal antibody (alemtuzumab), in a small uncontrolled study slowed down disease progression for a six-month period. Repeated muscle biopsies showed the reduction of T-cells in the muscle and the suppression of some degeneration associated molecules. An effective therapeutic mean should act on both aspects of the pathomechanism, on the inflammatory and the degenerative processes as well.

Place, publisher, year, edition, pages
Budapest, Hungary: Literatura Medica Kiado, 2013
Keywords
sIBM, inflammation, degenerative process, amyloid precursor protein, endoplasmic reticulum, immunosuppressive treatment
National Category
Physiology
Identifiers
urn:nbn:se:liu:diva-92707 (URN)000317633400002 ()
Available from: 2013-05-16 Created: 2013-05-16 Last updated: 2018-01-11Bibliographically approved
Gati, I., Merkli, H., Pal, E. & Danielsson, O. (2012). External Ophthalmoplegia Associated With Hashimotos Thyroiditis and Recovered on Corticosteroid Treatment. The American journal of surgery, 344(2), 151-152
Open this publication in new window or tab >>External Ophthalmoplegia Associated With Hashimotos Thyroiditis and Recovered on Corticosteroid Treatment
2012 (English)In: The American journal of surgery, ISSN 0002-9629, E-ISSN 1538-2990, Vol. 344, no 2, p. 151-152Article in journal (Refereed) Published
Abstract [en]

Five-year follow-up of a young male patient is presented. Total external ophthalmoplegia developed 1 week after an upper respiratory tract infection. After 3 years of the course, hyperthyreosis and clinical signs of thyroid-associated ophthalmopathy occurred. Hashimotos thyroiditis and ultrastructural signs of mitochondrial damage of striated muscle were found by histological investigations. The paresis of the external ocular muscles recovered after long-term corticosteroid treatment. On the basis of clinical symptoms and histological results, the authors supposed that an immunological reaction had caused mitochondrial damage in the striated muscles, which also resulted in thyroiditis. This case history points that autoimmune mechanism more frequently might participate in the pathogenesis of chronic external ophthalmoplegia, and the symptoms might precede organ-specific or perhaps systemic autoimmune disorders.

Place, publisher, year, edition, pages
Lippincott, Williams and Wilkins, 2012
Keywords
External ophthalmoplegia, Hashimotos thyroiditis, Corticosteroid treatment
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-81506 (URN)10.1097/MAJ.0b013e31824d4d93 (DOI)000306966300015 ()
Available from: 2012-09-18 Created: 2012-09-18 Last updated: 2017-12-07
Gati, I., Danielsson, O., Gunnarsson, C., Vrethem, M., Häggqvist, B., Fredriksson, B.-A. & Landtblom, A.-M. (2012). Letter: Bent Spine Syndrome: A Phenotype of Dysferlinopathy or a Symptomatic DYSF Gene Mutation Carrier [Letter to the editor]. European Neurology, 67(5), 300-302
Open this publication in new window or tab >>Letter: Bent Spine Syndrome: A Phenotype of Dysferlinopathy or a Symptomatic DYSF Gene Mutation Carrier
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2012 (English)In: European Neurology, ISSN 0014-3022, E-ISSN 1421-9913, Vol. 67, no 5, p. 300-302Article in journal, Letter (Other academic) Published
Abstract [en]

n/a

Place, publisher, year, edition, pages
Karger, 2012
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-77740 (URN)10.1159/000336265 (DOI)000303444900009 ()
Available from: 2012-05-28 Created: 2012-05-28 Last updated: 2017-12-07
Danielsson, O., Gati, I., Lindvall, B. & Ernerudh, J. (2011). CLASSIFICATION AND VALUE OF EXTENDED PATHOLOGICAL WORK UP OF 99 CONSECUTIVE PATIENTS WITH MORPHOLOGICAL FINDINGS OF INFLAMMATORY MYOPATHY in EUROPEAN JOURNAL OF NEUROLOGY, vol 18, issue SI, pp 49-49. In: EUROPEAN JOURNAL OF NEUROLOGY: (pp. 49-49). Wiley-Blackwell, 18(SI)
Open this publication in new window or tab >>CLASSIFICATION AND VALUE OF EXTENDED PATHOLOGICAL WORK UP OF 99 CONSECUTIVE PATIENTS WITH MORPHOLOGICAL FINDINGS OF INFLAMMATORY MYOPATHY in EUROPEAN JOURNAL OF NEUROLOGY, vol 18, issue SI, pp 49-49
2011 (English)In: EUROPEAN JOURNAL OF NEUROLOGY, Wiley-Blackwell , 2011, Vol. 18, no SI, p. 49-49Conference paper, Published paper (Refereed)
Abstract [en]

n/a

Place, publisher, year, edition, pages
Wiley-Blackwell, 2011
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-71077 (URN)000294806600103 ()
Available from: 2011-09-30 Created: 2011-09-30 Last updated: 2013-11-07
Gati, I., Danielsson, O., Vrethem, M., Lindehammar, H., Lindvall, B., Häggqvist, B., . . . Landtblom, A.-M. (2011). SENSORY ATAXIC NEUROPATHY WITH DYSARTHRIA/DYSPHAGIA AND OPHTHALMOPLEGIA (SANDO) - CASE HISTORIES in EUROPEAN JOURNAL OF NEUROLOGY, vol 18, issue SI, pp 282-282. In: EUROPEAN JOURNAL OF NEUROLOGY (pp. 282-282). Wiley-Blackwell, 18(SI)
Open this publication in new window or tab >>SENSORY ATAXIC NEUROPATHY WITH DYSARTHRIA/DYSPHAGIA AND OPHTHALMOPLEGIA (SANDO) - CASE HISTORIES in EUROPEAN JOURNAL OF NEUROLOGY, vol 18, issue SI, pp 282-282
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2011 (English)In: EUROPEAN JOURNAL OF NEUROLOGY, Wiley-Blackwell , 2011, Vol. 18, no SI, p. 282-282Conference paper, Published paper (Refereed)
Abstract [en]

n/a

Place, publisher, year, edition, pages
Wiley-Blackwell, 2011
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-71080 (URN)000294806600516 ()
Available from: 2011-09-30 Created: 2011-09-30 Last updated: 2012-04-03
Gati, I., Danielsson, O., Betmark, T., Ernerudh, J., Öllinger, K. & Dizdar (Dizdar Segrell), N. (2010). Culturing of diagnostic muscle biopsies as spheroid-like structures: a pilot study of morphology and viability. Neurological Research, 32(6), 650-655
Open this publication in new window or tab >>Culturing of diagnostic muscle biopsies as spheroid-like structures: a pilot study of morphology and viability
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2010 (English)In: Neurological Research, ISSN 0161-6412, E-ISSN 1743-1328, Vol. 32, no 6, p. 650-655Article in journal (Refereed) Published
Abstract [en]

Objective: The aim of this study was to establish three-dimensional cultures originating from muscle biopsies and evaluate the viability and morphology. Method: Muscle biopsies from patients with suspected neuromuscular disorders were obtained and established as primary muscle tissue cultures. Tissue pieces, 1-2 mm of diameters, were placed in culture medium and subjected to sporadic stirring to prevent attachment and outgrowth as monolayer cells. Morphology and ability to attach to the surface were investigated by light microscopy. Viability was evaluated by Tc-99m-tetrofosmin uptake. After 1 month, histology was evaluated by light microscopy and immunocytochemistry. The findings of a healthy muscle and a dystrophic muscle were compared. Results: Initially, the tissue pieces were unshaped but formed spheroid-like structures during the culture period. For dystrophic muscle, attachment capacity to the surface was initially potent and decreased during the culture period, whereas control muscle showed weak attachment from the start that increased during the culture period. The uptake of Tc-99m-tetrofosmin increased in control muscle, while it decreased in dystrophic muscle, during the culture period. The histological investigation demonstrated larger destruction of myofiber, weaker satellite cell activation and reduced myofiber regeneration in the dystrophic muscle as compared to the control muscle. Conclusion: The cellular components of the muscle tissue can survive and proliferate as spheroid-like primary cultures. The cellular composition resembles the in vivo condition, which allows studies of degeneration of the original fibers, and activation and proliferation of the satellite cells. The culture system may provide better understanding of the degeneration and regeneration processes in different muscle disorders and allow investigations of pharmacological interventions.

Place, publisher, year, edition, pages
Forefront Publishing Group, 2010
Keywords
Muscle biopsy; primary tissue culture; spheroid-like structure
National Category
Engineering and Technology
Identifiers
urn:nbn:se:liu:diva-58289 (URN)10.1179/016164109X12464612122579 (DOI)000279001300013 ()
Available from: 2010-08-10 Created: 2010-08-09 Last updated: 2018-01-12
Gati, I., Danielsson, O., Betmark, T., Ernerudh, J., Öllinger, K. & Dizdar Segrell, N. (2007). Effects of inhibitors of the arachidonic acid cascade on primary muscle culture from a Duchenne muscular dystrophy patient. Prostaglandins, Leukotrienes and Essential Fatty Acids, 77(3-4), 217-223
Open this publication in new window or tab >>Effects of inhibitors of the arachidonic acid cascade on primary muscle culture from a Duchenne muscular dystrophy patient
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2007 (English)In: Prostaglandins, Leukotrienes and Essential Fatty Acids, ISSN 0952-3278, E-ISSN 1532-2823, Vol. 77, no 3-4, p. 217-223Article in journal (Refereed) Published
Abstract [en]

The aim of this study was to elucidate the mechanisms of action for potential targets of therapeutic intervention related to the arachidonic acid cascade in muscular dystrophy. Primary cultures from a Duchenne patient were used to study the expression of dystrophin-1, utrophin, desmin, neonatal myosin heavy chain (MHCn) and Bcl-2 during inhibition of phospholipase A2 (PLA2), cyclooxygenase (COX) and lipoxygenase (LOX). Hypo-osmotic treatment was applied in order to trigger Ca2+ influx and PLA2 activity. Inhibition of PLA2 and LOX with prednisolone and nordihydroguaiaretic acid (NDGA) caused a semi-quantitative increase of utrophin and Bcl-2-, and a dose-dependent, quantitative increase of desmin expression, an effect that was augmented by hypo-osmotic treatment. Our results indicate that LOX inhibitors, similarly to corticosteroids, can be beneficial in the treatment of muscular dystrophies. © 2007 Elsevier Ltd. All rights reserved.

National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-40845 (URN)10.1016/j.plefa.2007.10.002 (DOI)54297 (Local ID)54297 (Archive number)54297 (OAI)
Available from: 2009-10-10 Created: 2009-10-10 Last updated: 2018-01-12
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