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de Muinck, Ebo
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Publications (10 of 26) Show all publications
Sherrell, P., Cieślar-Pobuda, A., Silverå Ejneby, M., Sammalisto, L., Gelmi, A., de Muinck, E., . . . Rafat, M. (2017). Rational Design of a Conductive Collagen Heart Patch. Macromolecular Bioscience, 17(7), Article ID 1600446.
Open this publication in new window or tab >>Rational Design of a Conductive Collagen Heart Patch
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2017 (English)In: Macromolecular Bioscience, ISSN 1616-5187, E-ISSN 1616-5195, Vol. 17, no 7, article id 1600446Article in journal (Refereed) Published
Abstract [en]

Cardiovascular diseases, including myocardial infarction, are the cause of significant morbidity and mortality globally. Tissue engineering is a key emerging treatment method for supporting and repairing the cardiac scar tissue caused by myocardial infarction. Creating cell supportive scaffolds that can be directly implanted on a myocardial infarct is an attractive solution. Hydrogels made of collagen are highly biocompatible materials that can be molded into a range of shapes suitable for cardiac patch applications. The addition of mechanically reinforcing materials, carbon nanotubes, at subtoxic levels allows for the collagen hydrogels to be strengthened, up to a toughness of 30 J m-1 and a two to threefold improvement in Youngs' modulus, thus improving their viability as cardiac patch materials. The addition of carbon nanotubes is shown to be both nontoxic to stem cells, and when using single-walled carbon nanotubes, supportive of live, beating cardiac cells, providing a pathway for the further development of a cardiac patch.

Place, publisher, year, edition, pages
Wiley-VCH Verlagsgesellschaft, 2017
Keywords
Carbon nanotube, Collagen, Hydrogel, Myocardial infarction, Stem cell
National Category
Medical Engineering
Identifiers
urn:nbn:se:liu:diva-136817 (URN)10.1002/mabi.201600446 (DOI)000405566300004 ()28322510 (PubMedID)2-s2.0-85016390421 (Scopus ID)
Note

Funding agencies: Linkoping Initiative in Life Science Technologies (LIST); Central ALF Matching Grant from Landstinget i Ostergotland [LIO-344071]; European Research Agency [304209]; GeCONiI [POIG.02.03.01-24-099/13]

Available from: 2017-04-27 Created: 2017-04-27 Last updated: 2018-04-09Bibliographically approved
Gelmi, A., Cieslar-Pobuda, A., de Muinck, E., Los, M. J., Rafat, M. & Jager, E. (2016). Direct Mechanical Stimulation of Stem Cells: A Beating Electromechanically Active Scaffold for Cardiac Tissue Engineering. Advanced Healthcare Materials, 5(12), 1471-1480
Open this publication in new window or tab >>Direct Mechanical Stimulation of Stem Cells: A Beating Electromechanically Active Scaffold for Cardiac Tissue Engineering
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2016 (English)In: Advanced Healthcare Materials, ISSN 2192-2640, E-ISSN 2192-2659, Vol. 5, no 12, p. 1471-1480Article in journal (Refereed) Published
Abstract [en]

The combination of stem cell therapy with a supportive scaffold is a promising approach to improving cardiac tissue engineering. Stem cell therapy can be used to repair nonfunctioning heart tissue and achieve myocardial regeneration, and scaffold materials can be utilized in order to successfully deliver and support stem cells in vivo. Current research describes passive scaffold materials; here an electroactive scaffold that provides electrical, mechanical, and topographical cues to induced human pluripotent stem cells (iPS) is presented. The poly(lactic-co-glycolic acid) fiber scaffold coated with conductive polymer polypyrrole (PPy) is capable of delivering direct electrical and mechanical stimulation to the iPS. The electroactive scaffolds demonstrate no cytotoxic effects on the iPS as well as an increased expression of cardiac markers for both stimulated and unstimulated protocols. This study demonstrates the first application of PPy as a supportive electroactive material for iPS and the first development of a fiber scaffold capable of dynamic mechanical actuation.

Place, publisher, year, edition, pages
Wiley-Blackwell Publishing Inc., 2016
Keywords
actuators; conductive polymers; scaffolds; stem cells; tissue engineering
National Category
Biophysics
Identifiers
urn:nbn:se:liu:diva-130427 (URN)10.1002/adhm.201600307 (DOI)000379550400010 ()27126086 (PubMedID)
Note

Funding Agencies|Linkoping University, Integrative Regenerative Medicine (IGEN) Center; Swedish Research Council [VR-2014-3079]; COST-Action [MP1003]; Knut och Alice Wallenberg Commemorative Fund; GeCONiI [POIG.02.03.01-24-099/13]; European Research Agency

Available from: 2016-08-07 Created: 2016-08-05 Last updated: 2018-10-11Bibliographically approved
Good, E., Länne, T., Wilhelm, E., Perk, J., Jaarsma, T. & de Muinck, E. (2016). High-grade carotid artery stenosis: A forgotten area in cardiovascular risk management. European Journal of Preventive Cardiology, 23(13), 1453-1460
Open this publication in new window or tab >>High-grade carotid artery stenosis: A forgotten area in cardiovascular risk management
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2016 (English)In: European Journal of Preventive Cardiology, ISSN 2047-4873, E-ISSN 2047-4881, Vol. 23, no 13, p. 1453-1460Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: Patients with high-grade (≥70%) carotid artery stenosis (CAS) rank in the highest risk category for future cardiovascular (CV) events, but the quality of cardiovascular risk management in this patient group is unknown.

DESIGN: Cross-sectional retrospective study.

METHODS: Data were collected for all patients diagnosed with high-grade CAS in Östergötland county, Sweden between 1 January 2009 and 31 July 2012 regarding the quality of cardiovascular risk management, co-morbidity and outcomes during the 2-year follow-up period after a diagnosis of CAS with a carotid ultrasound scan. Patients were included regardless of whether they underwent carotid endarterectomy (CEA).

RESULTS: A total of 393 patients with CAS were included in the study; 133 (33.8%) underwent CEA and 260 (66.2%) were assigned to a conservative management (CM) group. In both groups of patients the prescription of platelet inhibitors, statins and antihypertensive drugs increased significantly (p < 0.001) after diagnosis. However treatment targets were not met in the majority of patients and the low-density lipoprotein level was on target in only 13.5% of patients. During follow-up, low-density lipoprotein levels were not measured in 19.8% of patients who underwent CEA and 44.2% of patients in the CM group (p < 0.001); HbA1c was not measured in 24.4% of patients with diabetes in the CEA group and in 18.8% of patients in the CM group (p = 0.560). There was no documentation of counselling on diet, exercise, smoking cessation or adherence to medication. The combined clinical event rate (all-cause mortality, cardiovascular mortality and non-fatal cardiovascular events) was high in both groups (CEA 36.8% and CM 36.9%; p = 1.00) with no difference in the occurrence of ipsilateral ischaemic stroke.

CONCLUSIONS: The clinical event rate was high in patients with high-grade CAS and the management of cardiovascular risk was deficient in all aspects.

Place, publisher, year, edition, pages
Sage Publications, 2016
Keywords
High-grade carotid stenosis, atherosclerosis, cardiovascular disease, cardiovascular risk management, secondary prevention
National Category
Clinical Medicine
Identifiers
urn:nbn:se:liu:diva-129961 (URN)10.1177/2047487316632629 (DOI)000382655100013 ()26879568 (PubMedID)
Note

Funding agencies. Linkoping University Hospital [LIO-417951]

Available from: 2016-07-02 Created: 2016-07-02 Last updated: 2019-06-27Bibliographically approved
Gustafsson, H., Hallbeck, M., Lindgren, M., Kolbun, N., Jonson, M., Engström, M., . . . Zachrisson, H. (2015). Visualization of oxidative stress in ex vivo biopsies using electron paramagnetic resonance imaging. Magnetic Resonance in Medicine, 73(4), 1682-1691
Open this publication in new window or tab >>Visualization of oxidative stress in ex vivo biopsies using electron paramagnetic resonance imaging
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2015 (English)In: Magnetic Resonance in Medicine, ISSN 0740-3194, E-ISSN 1522-2594, Vol. 73, no 4, p. 1682-1691Article in journal (Refereed) Published
Abstract [en]

PURPOSE: The purpose of this study was to develop an X-Band electron paramagnetic resonance imaging protocol for visualization of oxidative stress in biopsies.

METHODS: The developed electron paramagnetic resonance imaging protocol was based on spin trapping with the cyclic hydroxylamine spin probe 1-hydroxy-3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine and X-Band EPR imaging. Computer software was developed for deconvolution and back-projection of the EPR image. A phantom containing radicals of known spatial characteristic was used for evaluation of the developed protocol. As a demonstration of the technique electron paramagnetic resonance imaging of oxidative stress was performed in six sections of atherosclerotic plaques. Histopathological analyses were performed on adjoining sections.

RESULTS: The developed computer software for deconvolution and back-projection of the EPR images could accurately reproduce the shape of a phantom of known spatial distribution of radicals. The developed protocol could successfully be used to image oxidative stress in six sections of the three ex vivo atherosclerotic plaques.

CONCLUSIONS: We have shown that oxidative stress can be imaged using a combination of spin trapping with the cyclic hydroxylamine spin probe cyclic hydroxylamine spin probe 1-hydroxy-3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine and X-Band EPR imaging. A thorough and systematic evaluation on different types of biopsies must be performed in the future to validate the proposed technique. Magn Reson Med, 2014.

National Category
Clinical Medicine
Identifiers
urn:nbn:se:liu:diva-113407 (URN)10.1002/mrm.25267 (DOI)000351685900035 ()24753234 (PubMedID)
Available from: 2015-01-19 Created: 2015-01-19 Last updated: 2017-12-05
Moreno, R., Romu, T., Dahlqvist Leinhard, O., Borga, M. & de Muinck, E. (2013). Effects of denoising in the estimation of T2* from images acquired through Dixon imaging. In: : . Paper presented at ISMRM 21st Annual Meeting & Exhibition, 20-26 April 2013, Salt Lake City, USA.
Open this publication in new window or tab >>Effects of denoising in the estimation of T2* from images acquired through Dixon imaging
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2013 (English)Conference paper, Poster (with or without abstract) (Other academic)
Keywords
MRI, Dixon imaging, Fat and water decomposition, T2*, image denoising, non-stationary Gaussian noise, non-local means
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-92791 (URN)
Conference
ISMRM 21st Annual Meeting & Exhibition, 20-26 April 2013, Salt Lake City, USA
Available from: 2013-05-22 Created: 2013-05-22 Last updated: 2019-06-14Bibliographically approved
Koppal, S., Moreno, R., Dyverfeldt, P., Warntjes, M. & de Muinck, E. (2013). Optimal validering av MR-bildtagning av aterosklerotiska plack genom användning av multi-modal MR och 3D histologi. In: : . Paper presented at Röntgenveckan 2013, 2-6 september 2013, Uppsala.
Open this publication in new window or tab >>Optimal validering av MR-bildtagning av aterosklerotiska plack genom användning av multi-modal MR och 3D histologi
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2013 (Swedish)Conference paper, Poster (with or without abstract) (Other academic)
Abstract [sv]

BAKGRUND: Magnetkamera (MR) kan identifiera aterosklerotiska plack som löper risk att brista och därmed orsaka stroke eller hjärtinfarkt. Metoden är dock bristfälligt validerad på grund av den osäkerhet som uppstår då 2D histologiska snitt ska registreras med 3D MR-bilder.

SYFTE: Att optimera validering av MR-bildtagning av aterosklerotiska plack genom användning av multi-modal MR och 3D histologi.

MATERIAL och METOD: Patienter som skulle opereras för att avlägsna aterosklerotiska plack från arteria karotis genomgick dedikerad plack-MR där följande parametrar undersöktes: plackets fettinnehåll, blödning inuti placket och maximal intensitet av turbulent blodflöde. Undersökningarna gjordes med en Philips 3T MR-kamera: (a) 4-punkt Dixon 3D gradient-eko, (b) T1-viktad spin-eko, (c) 4D fas-kontrast. Upplösningen var 0.6x0.6x0.7mm, 0.35x0.35x3mm respektive 1.14x1.25x1.14mm x 25ms. Vatten-, fett- and R2*-bilder (blödning) beräknades utifrån Dixon-sekvensen.Efter operation bäddades placken in i paraffin och enface-bilder togs varje 50µm i Z-riktning. Bilderna registrerades i ImageJ/Fiji och användes för att bygga en 3D-volym av placket. Vid varje 200µm togs snitt för biologiska markörer och histologiska färgningar. De färgade snitten registrerades med motsvarande enface-bilder. Detta resulterade i 3D-volymer med en upplösning på 1.02x1.02x200µm. Den histologiska 3D-volymen registrerades manuellt med uppsamplade och co-registrerade MR-bilder.

RESULTAT: T1-viktade bilder var bäst för registrering av plack inom varje snitt. Registrering av kärlets lumen optimerades genom en kombination av 4D fas-kontrast, det första Dixon-ekot och vatten-bilder. Registrering av fett och R2* från MR-bilder med fett och blödning från 3D histologi uppvisade god överensstämmelse.

SLUTSATS: Optimal validering av MR-bilder av aterosklerotiska plack kan åstadkommas genom att kombinera olika anatomiska landmärken från multimodala MR-bilder av plack och 3D-histologi. Genom att använda 3D-histologi korrigerar man för registreringsproblem som är relaterade till ”out-of-plane” vinklingar av vävnadssnitt och krympning och deformering till följd av histologiskt bearbetning av placket. Den detaljerade biologiska informationen från 3D-histologi kan förväntas förstärka fynden från in vivo MR-bilder.

National Category
Cardiac and Cardiovascular Systems Radiology, Nuclear Medicine and Medical Imaging
Identifiers
urn:nbn:se:liu:diva-97714 (URN)
Conference
Röntgenveckan 2013, 2-6 september 2013, Uppsala
Funder
Swedish Heart Lung Foundation, 20100460
Available from: 2013-09-20 Created: 2013-09-20 Last updated: 2013-09-26Bibliographically approved
Moreno, R., Koppal, S. & de Muinck, E. (2013). Robust Estimation of Distance Between Sets of Points. Pattern Recognition Letters, 34(16), 2192-2198
Open this publication in new window or tab >>Robust Estimation of Distance Between Sets of Points
2013 (English)In: Pattern Recognition Letters, ISSN 0167-8655, E-ISSN 1872-7344, Vol. 34, no 16, p. 2192-2198Article in journal (Refereed) Published
Abstract [en]

This paper proposes a new methodology for computing Hausdorff distances between sets of points in a robust way. In a first step, robust nearest neighbor distance distributions between the two sets of points are obtained by considering reliability measures in the computations through a Monte Carlo scheme. In a second step, the computed distributions are operated using random variables algebra in order to obtain probability distributions of the average, minimum or maximum distances. In the last step, different statistics are computed from these distributions. A statistical test of significance, the nearest neighbor index, in addition to the newly proposed divergence and clustering indices are used to compare the computed measurements with respect to values obtained by chance. Results on synthetic and real data show that the proposed method is more robust than the standard Hausdorff distance. In addition, unlike previously proposed methods based on thresholding, it is appropriate for problems that can be modeled through point processes.

Place, publisher, year, edition, pages
Elsevier, 2013
Keywords
Spatial statistics; Distance estimation; Hausdorff distance; Nearest neighbor distance distribution
National Category
Cardiac and Cardiovascular Systems
Identifiers
urn:nbn:se:liu:diva-97282 (URN)10.1016/j.patrec.2013.08.012 (DOI)000333104500019 ()
Funder
Swedish Heart Lung Foundation, 20100460
Available from: 2013-09-06 Created: 2013-09-06 Last updated: 2017-12-06Bibliographically approved
Dilip Deb, K., Griffith, M., De Muinck, E. & Rafat, M. (2012). Nanotechnology in stem cells research: advances and applications. Frontiers in Bioscience, 17, 1747-1760
Open this publication in new window or tab >>Nanotechnology in stem cells research: advances and applications
2012 (English)In: Frontiers in Bioscience, ISSN 1093-9946, E-ISSN 1093-4715, Vol. 17, p. 1747-1760Article in journal (Refereed) Published
Abstract [en]

Human beings suffer from a myriad of disorders caused by biochemical or biophysical alteration of physiological systems leading to organ failure. For a number of these conditions, stem cells and their enormous reparative potential may be the last hope for restoring function to these failing organ or tissue systems. To harness the potential of stem cells for biotherapeutic applications, we need to work at the size scale of molecules and processes that govern stem cells fate. Nanotechnology provides us with such capacity. Therefore, effective amalgamation of nanotechnology and stem cells - medical nanoscience or nanomedicine - offers immense benefits to the human race. The aim of this paper is to discuss the role and importance of nanotechnology in stem cell research by focusing on several important areas such as stem cell visualization and imaging, genetic modifications and reprogramming by gene delivery systems, creating stem cell niche, and similar therapeutic applications.

Place, publisher, year, edition, pages
Frontiers in Bioscience, 2012
Keywords
Nanotechnology, Stem cells, Carbon nanotubes, Nanoparticles, Electron microscopy, Nanodiagnostics, Viral vectors, Review
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-75730 (URN)10.2741/4016 (DOI)000300054400011 ()
Available from: 2012-03-09 Created: 2012-03-09 Last updated: 2017-12-07Bibliographically approved
Eisenbrey, J. R., Sridharan, A., deMuinck, E. D., Doyley, M. M. & Forsberg, F. (2012). Parametric Subharmonic Imaging Using a Commercial Intravascular Ultrasound Scanner: An In Vivo Feasibility Study. Journal of ultrasound in medicine, 31(3), 361-371
Open this publication in new window or tab >>Parametric Subharmonic Imaging Using a Commercial Intravascular Ultrasound Scanner: An In Vivo Feasibility Study
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2012 (English)In: Journal of ultrasound in medicine, ISSN 0278-4297, E-ISSN 1550-9613, Vol. 31, no 3, p. 361-371Article in journal (Refereed) Published
Abstract [en]

Objectives— The feasibility of visualizing atherosclerotic plaque using parametric subharmonic intravascular ultrasound (IVUS) was investigated in vivo.                    

Methods— Atherosclerosis was induced in the aorta of 2 rabbits. Following injection of Definity (Lantheus Medical Imaging, North Billerica, MA), radiofrequency IVUS signals were acquired at 40 MHz with a Galaxy IVUS scanner (Boston Scientific/Scimed, Natick, MA).        Subharmonic imaging (SHI; receiving at 20 MHz) was performed offline by applying an 8-order equalization filter. Contrast-to-tissue ratios (CTRs) were computed for the vessel relative to the plaque area over 4 time points. Contrast-to-tissue ratios were also calculated for the plaque-tissue and vessel-tissue from 4 tissue regions of interest at 4 time points. Finally, parametric images showing the cumulative maximum intensity (CMI), time to peak, perfusion (PER), and time-integrated intensity (TII) were generated for the fundamental and subharmonic data sets, and CTR measurements were repeated.                    

Results— Injection of the contrast agent resulted in improved delineation between plaque and the vessel lumen. Subharmonic imaging resulted in noticeable tissue suppression, although the intensity from the contrast agent was reduced. No improvement in the plaque to vessel lumen CTR was observed between the subharmonic and fundamental IVUS (2.1 ± 3.64 versus 2.2 ± 4.20; P = .5). However, the CTR for plaque-tissue was improved (11.8 ± 7.32 versus 9.9 ± 7.06; P < .0001) for SHI relative to fundamental imaging. Cumulative-maximum-intensity and TII maps of both fundamental and subharmonic data provided increased CTRs relative to nonparametric data sets (P < .002). Additionally, the CMI, PER, and TII of SHI IVUS showed significantly improved vessel-plaque CTRs relative to the fundamental (P < .04).                    

Conclusions— Parametric SHI IVUS of atherosclerotic plaque is feasible and improves the visualization of the plaque.

Place, publisher, year, edition, pages
American Institute for Ultrasound in Medicine, 2012
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-86767 (URN)
Available from: 2013-01-03 Created: 2013-01-03 Last updated: 2017-06-28
Gustafsson, H., Berg, K., Lindgren, M., Engström, M., De Muinck, E. & Zachrisson, H. (2011). Fe(3+) Heterogeneity in Ex Vivo Carotid Atherosclerotic Plaques. Paper presented at 18th Annual Meeting of the Society-for-Free-Radical-Biology-and-Medicine (SFRBM), Atlanta, GA, USA, Nov. 16-20, 2011. Free Radical Biology & Medicine, 51(Suppl. 1), S40-S40
Open this publication in new window or tab >>Fe(3+) Heterogeneity in Ex Vivo Carotid Atherosclerotic Plaques
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2011 (English)In: Free Radical Biology & Medicine, ISSN 0891-5849, E-ISSN 1873-4596, Vol. 51, no Suppl. 1, p. S40-S40Article in journal, Meeting abstract (Other academic) Published
Abstract [en]

n/a

Place, publisher, year, edition, pages
Elsevier, 2011
National Category
Biological Sciences
Identifiers
urn:nbn:se:liu:diva-72815 (URN)10.1016/j.freeradbiomed.2011.10.097 (DOI)000297036600090 ()
Conference
18th Annual Meeting of the Society-for-Free-Radical-Biology-and-Medicine (SFRBM), Atlanta, GA, USA, Nov. 16-20, 2011
Available from: 2011-12-09 Created: 2011-12-08 Last updated: 2017-12-08Bibliographically approved
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