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Bhai Mehta, Ratnesh
Alternative names
Publications (5 of 5) Show all publications
Atikuzzaman, M., Bhai Mehta, R., Fogelholm, J., Wright, D. & Rodriguez-Martinez, H. (2015). Mating induces the expression of immune- and pH-regulatory genes in the utero-vaginal junction containing mucosal sperm-storage tubuli of hens. Reproduction, 150(6), 473-483
Open this publication in new window or tab >>Mating induces the expression of immune- and pH-regulatory genes in the utero-vaginal junction containing mucosal sperm-storage tubuli of hens
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2015 (English)In: Reproduction, Vol. 150, no 6, p. 473-483Article in journal (Refereed) Published
Abstract [en]

The female chicken, as with other species with internal fertilization, can tolerate the presence of spermatozoa within specialized sperm-storage tubuli (SST) located in the mucosa of the utero-vaginal junction (UVJ) for days or weeks, without eliciting an immune response. To determine if the oviduct alters its gene expression in response to sperm entry, segments from the oviduct (UVJ, uterus, isthmus, magnum and infundibulum) of mated and unmated (control) hens, derived from an advanced inter-cross line between Red Junglefowl and White Leghorn, were explored 24 h after mating using cDNA microarray analysis. Mating shifted the expression of fifteen genes in the UVJ (53.33% immune-modulatory and 20.00% pH-regulatory) and seven genes in the uterus, none of the genes in the latter segment overlapping the former (with the differentially expressed genes themselves being less related to immune-modulatory function). The other oviductal segments did not show any significant changes. These findings suggest sperm deposition causes a shift in expression in the UVJ (containing mucosal SST) and the uterus for genes involved in immune-modulatory and pH-regulatory functions, both relevant for sperm survival in the hen's oviduct.

Place, publisher, year, edition, pages
Bioscientifica, 2015
National Category
Genetics
Identifiers
urn:nbn:se:liu:diva-122573 (URN)10.1530/REP-15-0253 (DOI)000365344400004 ()26370241 (PubMedID)
Note

Funding agencies: Research Council FORMAS, Stockholm [221-2011-512]; FORMAS [221-2012-667]; VR [621-2011-4802]

Available from: 2015-11-09 Created: 2015-11-09 Last updated: 2017-02-20
Svensson, J., Bhai Mehta, R., Lindau, R., Mirrasekhian, E., Rodriguez-Martinez, H., Berg, G., . . . Ernerudh, J. (2015). The Human Fetal Placenta Promotes Tolerance against the Semiallogeneic Fetus by Inducing Regulatory T Cells and Homeostatic M2 Macrophages. Journal of Immunology, 194(4), 1534-1544
Open this publication in new window or tab >>The Human Fetal Placenta Promotes Tolerance against the Semiallogeneic Fetus by Inducing Regulatory T Cells and Homeostatic M2 Macrophages
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2015 (English)In: Journal of Immunology, ISSN 0022-1767, E-ISSN 1550-6606, Vol. 194, no 4, p. 1534-1544Article in journal (Refereed) Published
Abstract [en]

A successful pregnancy requires that the maternal immune system is instructed to a state of tolerance to avoid rejection of the semiallogeneic fetal-placental unit. Although increasing evidence supports that decidual (uterine) macrophages and regulatory T cells (Tregs) are key regulators of fetal tolerance, it is not known how these tolerogenic leukocytes are induced. In this article, we show that the human fetal placenta itself, mainly through trophoblast cells, is able to induce homeostatic M2 macrophages and Tregs. Placental-derived M-CSF and IL-10 induced macrophages that shared the CD14(+)CD163(+)CD206(+)CD209(+) phenotype of decidual macrophages and produced IL-10 and CCL18 but not IL-12 or IL-23. Placental tissue also induced the expansion of CD25(high)CD127(low)Foxp3(+) Tregs in parallel with increased IL-10 production, whereas production of IFN-gamma (Th1), IL-13 (Th2), and IL-17 (Th17) was not induced. Tregs expressed the suppressive markers CTLA-4 and CD39, were functionally suppressive, and were induced, in part, by IL-10, TGF-beta, and TRAIL. Placental-derived factors also limited excessive Th cell activation, as shown by decreased HLA-DR expression and reduced secretion of Th1-, Th2-, and Th17-associated cytokines. Thus, our data indicate that the fetal placenta has a central role in promoting the homeostatic environment necessary for successful pregnancy. These findings have implications for immune-mediated pregnancy complications, as well as for our general understanding of tissue-induced tolerance.

Place, publisher, year, edition, pages
American Association of Immunologists, 2015
National Category
Clinical Medicine Immunology in the medical area
Identifiers
urn:nbn:se:liu:diva-115319 (URN)10.4049/jimmunol.1401536 (DOI)000349462000017 ()25560409 (PubMedID)
Note

Funding Agencies|Medical Research Council [K2013-61X-22310-01-4]

Available from: 2015-03-13 Created: 2015-03-13 Last updated: 2018-01-11
Forsberg, A., Straka, E., Johansson, E., Mehta, R., Berg, G., Jenmalm, M. C., . . . Ernerudh, J. (2011). Plasticity and flexibility of T cells in human pregnancy in JOURNAL OF REPRODUCTIVE IMMUNOLOGY, vol 90, issue 2, pp 149-149. In: JOURNAL OF REPRODUCTIVE IMMUNOLOGY (pp. 149-149). Elsevier, 90(2)
Open this publication in new window or tab >>Plasticity and flexibility of T cells in human pregnancy in JOURNAL OF REPRODUCTIVE IMMUNOLOGY, vol 90, issue 2, pp 149-149
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2011 (English)In: JOURNAL OF REPRODUCTIVE IMMUNOLOGY, Elsevier , 2011, Vol. 90, no 2, p. 149-149Conference paper, Published paper (Refereed)
Abstract [en]

Introduction:Pregnancy challenges the immune system. Thus, tolerance to the semi-allogenic fetus must be supported while the mother and fetus still must be protected against infectious agents. Pregnancy is associated with a Th2 deviated immune system, away from a harmful Th1 associated immunity, although this may be a simplified view. Regulatory T cells (Tregs) are enriched in the uterus, but occur at normal frequency in the circulation. It has become increasingly evident that Tregs and T helper cells are not stably committed lineages but are plastic, showing close relationships between subsets. We hypothesize that an increased T cell flexibility in pregnancy can help to explain the paradox of simultaneous tolerance and strong antimicrobial responses. Our aim was to investigate whether the plasticity concept is applicable for the Treg subset, and if it involves the entire T helper population.

Material and methods: Isolated Tregs (CD4dimCD25high) and control cells (CD4+CD25−) from second trimester pregnant (n = 14) and non-pregnant women (n = 14) were stimulated for 24 h with plate-bound anti-CD3/anti-CD28. Signature gene and protein expression of each T cell subset was measured using transcription factor expression by real time-PCR and multiplex bead array of cell culture supernatants, respectively. The whole PBMC fraction is also used in ongoing experiments and either stimulated with plate-bound anti-CD3/anti-CD28 or with the Th1, Th2 and Th17 deviating microbial agents PPD (Th1), TT (Th2) and C. albicans hyphae (Th17). After culturing, the cells are stained for intracellular transcription factors associated with Th1, Th2, Th17 and Treg immunity.

Results: Stimulated Tregs from pregnant compared to non-pregnant women showed significantly higher levels of markers for Treg cells (Foxp3 mRNA), Th2 cells (GATA-3 mRNA and IL4 protein) and a tendency to increase in markers of Th17 (RORC mRNA and IL-17 protein), whereas Th1 markers (Tbet mRNA and IFN-γ) showed no difference between pregnant and non-pregnant women. Further, ongoing studies may reveal if the entire T helper population shows a higher degree of responsiveness during pregnancy.

Conclusions: Our results imply an increased plasticity of the Treg population during pregnancy, suggesting that Treg cells are able to switch to a Th2/Th17-like phenotype, depending on current demands of tolerance or infectious threats.

Place, publisher, year, edition, pages
Elsevier, 2011
Keywords
Pregnancy, T cell plasticity, Tolerance, Infectious agents
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-70335 (URN)10.1016/j.jri.2011.06.034 (DOI)000293873500032 ()
Available from: 2011-09-02 Created: 2011-09-02 Last updated: 2011-09-06
Ernerudh, J., Forsberg, A., Straka, E., Johansson, E., Bhai Mehta, R., Svensson, J., . . . Jenmalm, M. (2011). T helper cells and T helper cell plasticity in pregnancy in JOURNAL OF REPRODUCTIVE IMMUNOLOGY, vol 90, issue 2, pp 131-131. In: JOURNAL OF REPRODUCTIVE IMMUNOLOGY (pp. 131-131). Elsevier, 90(2)
Open this publication in new window or tab >>T helper cells and T helper cell plasticity in pregnancy in JOURNAL OF REPRODUCTIVE IMMUNOLOGY, vol 90, issue 2, pp 131-131
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2011 (English)In: JOURNAL OF REPRODUCTIVE IMMUNOLOGY, Elsevier , 2011, Vol. 90, no 2, p. 131-131Conference paper, Published paper (Refereed)
Abstract [en]

n/a

Place, publisher, year, edition, pages
Elsevier, 2011
Keywords
Pregnancy, T helper cell, Tolerance
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-70334 (URN)10.1016/j.jri.2011.06.003 (DOI)000293873500002 ()
Available from: 2011-09-02 Created: 2011-09-02 Last updated: 2012-03-25
Bhai Mehta, R., Mirrasekhian, E., Svensson, J., Freland, S., Berg, G., Sharma, S., . . . Ernerudh, J. (2011). Trophoblast cells in immune regulation: modulation of macrophage polarization and production of IL-35 in JOURNAL OF REPRODUCTIVE IMMUNOLOGY, vol 90, issue 2, pp 165-165. In: JOURNAL OF REPRODUCTIVE IMMUNOLOGY (pp. 165-165). Elsevier, 90(2)
Open this publication in new window or tab >>Trophoblast cells in immune regulation: modulation of macrophage polarization and production of IL-35 in JOURNAL OF REPRODUCTIVE IMMUNOLOGY, vol 90, issue 2, pp 165-165
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2011 (English)In: JOURNAL OF REPRODUCTIVE IMMUNOLOGY, Elsevier , 2011, Vol. 90, no 2, p. 165-165Conference paper, Published paper (Refereed)
Abstract [en]

n/a

Place, publisher, year, edition, pages
Elsevier, 2011
Keywords
Trophoblast, Macrophage, Polarization, IL-35
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-70340 (URN)10.1016/j.jri.2011.06.061 (DOI)000293873500057 ()
Available from: 2011-09-02 Created: 2011-09-02 Last updated: 2011-09-02
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