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Polisetti, Naresh
Publications (5 of 5) Show all publications
Wickham, A. M., Islam, M. M., Mondal, D., Phopase, J., Sadhu, V., Tamás, É., . . . Griffith, M. (2014). Polycaprolactone–thiophene-conjugated carbon nanotube meshes as scaffolds for cardiac progenitor cells. Journal of Biomedical Materials Research. Part B - Applied biomaterials, 102(7), 1553-1561
Open this publication in new window or tab >>Polycaprolactone–thiophene-conjugated carbon nanotube meshes as scaffolds for cardiac progenitor cells
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2014 (English)In: Journal of Biomedical Materials Research. Part B - Applied biomaterials, ISSN 1552-4973, E-ISSN 1552-4981, Vol. 102, no 7, p. 1553-1561Article in journal (Refereed) Published
Abstract [en]

The myocardium is unable to regenerate itself after infarct, resulting in scarring and thinning of the heart wall. Our objective was to develop a patch to buttress and bypass the scarred area, while allowing regeneration by incorporated cardiac stem/progenitor cells (CPCs). Polycaprolactone (PCL) was fabricated as both sheets by solvent casting, and fibrous meshes by electrospinning, as potential patches, to determine the role of topology in proliferation and phenotypic changes to the CPCs. Thiophene-conjugated carbon nanotubes (T-CNTs) were incorporated to enhance the mechanical strength. We showed that freshly isolated CPCs from murine hearts neither attached nor spread on the PCL sheets, both with and without T-CNT. As electrospun meshes, however, both PCL and PCL/T-CNT supported CPC adhesion, proliferation, and differentiation. The incorporation of T-CNT into PCL resulted in a significant increase in mechanical strength but no morphological changes to the meshes. In turn, proliferation, but not differentiation, of CPCs into cardiomyocytes was enhanced in T-CNT containing meshes. We have shown that changing the topology of PCL, a known hydrophobic material, dramatically altered its properties, in this case, allowing CPCs to survive and differentiate. With further development, PCL/T-CNT meshes or similar patches may become a viable strategy to aid restoration of the postmyocardial infarction myocardium.

Place, publisher, year, edition, pages
John Wiley & Sons, 2014
Keywords
topology, carbon nanotubes, polycaprolactone, cardiac progenitor cells, electrospun meshes
National Category
Clinical Medicine Basic Medicine Physical Sciences
Identifiers
urn:nbn:se:liu:diva-111488 (URN)10.1002/jbm.b.33136 (DOI)000342963000020 ()24664884 (PubMedID)
Available from: 2014-10-19 Created: 2014-10-19 Last updated: 2018-01-11Bibliographically approved
Polisetti, N., McLaughlin, C. R., Vemuganti, G. K. & Griffith, M. (2013). Biomaterials-Enabled Regenerative Medicine in Corneal Applications (2ed.). In: Steinhoff, Gustav (Ed.), Regenerative Medicine: From Protocol to Patient (pp. 557-580). Springer Netherlands
Open this publication in new window or tab >>Biomaterials-Enabled Regenerative Medicine in Corneal Applications
2013 (English)In: Regenerative Medicine: From Protocol to Patient / [ed] Steinhoff, Gustav, Springer Netherlands, 2013, 2, p. 557-580Chapter in book (Other academic)
Abstract [en]

This book details the latest scientific and clinical knowledge in regenerative medicine. Coverage includes biology of tissue regeneration, stem cell science and technology, tissue engineering, biomaterials and nanotechnology, and regulation and ethics.

Place, publisher, year, edition, pages
Springer Netherlands, 2013 Edition: 2
Keywords
Regenerative Medicine, Regeneration, Stem Cell Research, Stem Cells physiology, Regenerative medicine
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-98703 (URN)10.1007/978-94-007-5690-8_22 (DOI)978-94-007-5689-2 (ISBN)978-94-007-5690-8 (ISBN)
Available from: 2013-10-11 Created: 2013-10-11 Last updated: 2014-11-11Bibliographically approved
Qian, H., Badaloni, A., Chiara, F., Stjernberg, J., Polisetti, N., Nihlberg, K., . . . Sigvardsson, M. (2013). Molecular Characterization of Prospectively Isolated Multipotent Mesenchymal Progenitors Provides New Insight into the Cellular Identity of Mesenchymal Stem Cells in Mouse Bone Marrow. Molecular and Cellular Biology, 33(4), 661-677
Open this publication in new window or tab >>Molecular Characterization of Prospectively Isolated Multipotent Mesenchymal Progenitors Provides New Insight into the Cellular Identity of Mesenchymal Stem Cells in Mouse Bone Marrow
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2013 (English)In: Molecular and Cellular Biology, ISSN 0270-7306, E-ISSN 1098-5549, Vol. 33, no 4, p. 661-677Article in journal (Refereed) Published
Abstract [en]

Despite great progress in the identification of mesenchymal stem cells (MSCs) from bone marrow (BM), our knowledge of their in vivo cellular identity remains limited. We report here that cells expressing the transcription factor Ebf2 in adult BM display characteristics of MSCs. The Ebf2(+) cells are highly clonal and physiologically quiescent. In vivo lineage-tracing experiments, single cell clone transplantations, and in vitro differentiation assays revealed their self-renewal and multilineage differentiation capacity. Gene expression analysis of the freshly sorted Ebf2(+) cells demonstrated the expression of genes previously reported to be associated with MSCs and the coexpression of multiple lineage-associated genes at the single-cell level. Thus, Ebf2 expression is not restricted to committed osteoblast progenitor cells but rather marks a multipotent mesenchymal progenitor cell population in adult mouse BM. These cells do not appear to completely overlap the previously reported MSC populations. These findings provide new insights into the in vivo cellular identity and molecular properties of BM mesenchymal stem and progenitor cells.

Place, publisher, year, edition, pages
American Society for Microbiology, 2013
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-92619 (URN)10.1128/MCB.01287-12 (DOI)000317267000002 ()
Note

Funding Agencies|Swedish Research Council|K2008-77PK-20879-01-22009-2675|Cancer-fonden|CAN 2009/1589CAN 2012-2015|AFA Insurance Regenerative Medicine Program||Faculty of Medicine at Linkoping University||

Available from: 2013-05-16 Created: 2013-05-14 Last updated: 2019-02-11
Griffith, M., Polisetti, N., Kuffova, L., Gallar, J., Forrester, J., Vemuganti, G. K. & Armin Fuchsluger, T. (2012). Regenerative Approaches as Alternatives to Donor Allografting for Restoration of Corneal Function. The Ocular Surface, 10(3), 170-183
Open this publication in new window or tab >>Regenerative Approaches as Alternatives to Donor Allografting for Restoration of Corneal Function
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2012 (English)In: The Ocular Surface, ISSN 1542-0124, Vol. 10, no 3, p. 170-183Article in journal (Refereed) Published
Abstract [en]

A range of alternatives to human donor tissue for corneal transplantation are being developed to address the shortfall of good quality tissues as well as the clinical conditions for which allografting is contraindicated. Classical keratoprostheses, commonly referred to as artificial corneas, are being used clinically to replace minimal corneal function. However, they are used only as last resorts, as they are associated with significant complications, such as extrusion/rejection, glaucoma, and retinal detachment. The past few years have seen significant developments in technologies designed to replace part or the full thickness of damaged or diseased corneas with materials that encourage regeneration to different extents. This review describes selected examples of these corneal substitutes, which range from cell-based regenerative strategies to keratoprostheses with regenerative capabilities via tissue-engineered scaffolds pre-seeded with stem cells. It is unlikely that one corneal substitute will be best for all indications, but taken together, the various approaches may soon be able to supplement the supply of human donor corneas for transplantation or allow restoration of diseased or damaged corneas that cannot be treated by currently available techniques.

Place, publisher, year, edition, pages
Elsevier, 2012
Keywords
biomaterial scaffolds, corneal transplantation, keratoprostheses, stem cells, regeneration
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-81835 (URN)10.1016/j.jtos.2012.04.004 (DOI)000307262200005 ()
Note

Funding Agencies|Swedish Research Council, County Council of Ostergotland, Sweden||Canadian Stem Cell Network||NSERC Canada|SAF2011-22500|Spanish Ministerio de Economia y Competitividad||

Available from: 2012-09-25 Created: 2012-09-24 Last updated: 2013-12-17
Alarcon, E. I., Udekwu, K., Skog, M., Pacioni, N., Stamplecoskie, K. G., Gonzalez-Bejar, M., . . . Scaiano, J. C. (2012). The biocompatibility and antibacterial properties of collagen-stabilized, photochemically prepared silver nanoparticles. Biomaterials, 33(19), 4947-4956
Open this publication in new window or tab >>The biocompatibility and antibacterial properties of collagen-stabilized, photochemically prepared silver nanoparticles
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2012 (English)In: Biomaterials, ISSN 0142-9612, E-ISSN 1878-5905, Vol. 33, no 19, p. 4947-4956Article in journal (Refereed) Published
Abstract [en]

Spherical 3.5 nm diameter silver nanoparticles (AgNP) stabilized in type I collagen (AgNP@collagen) were prepared in minutes (5-15 min) at room temperature by a photochemical method initiated by UVA irradiation of a water-soluble non-toxic benzoin. This biocomposite was examined to evaluate its biocompatibility and its anti-bacterial properties and showed remarkable properties. Thus, while keratinocytes and fibroblasts were not affected by AgNP@collagen, it was bactericidal against Bacillus megaterium and E. coli but only bacteriostatic against S. epidermidis. In particular, the bactericidal properties displayed by AgNP@collagen were proven to be due to AgNP in AgNP@collagen, rather than to released silver ions, since equimolar concentrations of Ag are about four times less active than AgNP@collagen based on total Ag content. This new biocomposite was stable over a remarkable range of NaCl, phosphate, and 2-(N-morpholino)ethanesulfonic acid concentrations and for over one month at 4 degrees C. Circular dichroism studies show that the conformation of collagen in AgNP@collagen remains intact. Finally, we have compared the properties of AgNP@collagen with a similar biocomposite prepared using alpha-poly-L-Lysine and also with citrate stabilized AgNP; neither of these materials showed comparable biocompatibility, stability, or anti-bacterial activity.

Place, publisher, year, edition, pages
Elsevier, 2012
Keywords
Silver nano particles, Type-I collagen, Poly-L-Lysine, Cell toxicity, Antimicrobial activity
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-78260 (URN)10.1016/j.biomaterials.2012.03.033 (DOI)000303966100018 ()
Note
Funding Agencies|Natural Sciences and Engineering Research Council (NSERC, Canada)||Available from: 2012-06-08 Created: 2012-06-08 Last updated: 2017-12-07
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