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Shavanova, K., Bakakina, Y., Burkova, I., Shtepliuk, I., Viter, R., Ubelis, A., . . . Khranovskyy, V. (2016). Application of 2D Non-Graphene Materials and 2D Oxide Nanostructures for Biosensing Technology. Sensors, 16(2)
Open this publication in new window or tab >>Application of 2D Non-Graphene Materials and 2D Oxide Nanostructures for Biosensing Technology
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2016 (English)In: Sensors, ISSN 1424-8220, E-ISSN 1424-8220, Vol. 16, no 2Article, review/survey (Refereed) Published
Abstract [en]

The discovery of graphene and its unique properties has inspired researchers to try to invent other two-dimensional (2D) materials. After considerable research effort, a distinct "beyond graphene" domain has been established, comprising the library of non-graphene 2D materials. It is significant that some 2D non-graphene materials possess solid advantages over their predecessor, such as having a direct band gap, and therefore are highly promising for a number of applications. These applications are not limited to nano- and opto-electronics, but have a strong potential in biosensing technologies, as one example. However, since most of the 2D non-graphene materials have been newly discovered, most of the research efforts are concentrated on material synthesis and the investigation of the properties of the material. Applications of 2D non-graphene materials are still at the embryonic stage, and the integration of 2D non-graphene materials into devices is scarcely reported. However, in recent years, numerous reports have blossomed about 2D material-based biosensors, evidencing the growing potential of 2D non-graphene materials for biosensing applications. This review highlights the recent progress in research on the potential of using 2D non-graphene materials and similar oxide nanostructures for different types of biosensors (optical and electrochemical). A wide range of biological targets, such as glucose, dopamine, cortisol, DNA, IgG, bisphenol, ascorbic acid, cytochrome and estradiol, has been reported to be successfully detected by biosensors with transducers made of 2D non-graphene materials.

Place, publisher, year, edition, pages
MDPI AG, 2016
Keywords
transition metal dichalcogenides; transducers; beyond graphene; biosensors; two-dimensional materials; two-dimensional oxides; transition metal oxides
National Category
Chemical Sciences
Identifiers
urn:nbn:se:liu:diva-126850 (URN)10.3390/s16020223 (DOI)000371787800102 ()26861346 (PubMedID)
Note

Funding Agencies|EC FP-7 International Research Staff Exchange Scheme (IRSES) Grant [318520]; Linkoping Linnaeus Initiative for Novel Functional Materials (LiLi-NFM); European Union [604391]; Swedish Research Council (VR) Marie Sklodowska Curie International Career Grant [2015-00679]

Available from: 2016-04-05 Created: 2016-04-05 Last updated: 2017-11-30
Zhybak, M., Beni, V., Vagin, M., Dempsey, E., Turner, A. & Korpan, Y. (2016). Creatinine and urea biosensors based on a novel ammonium ion-selective copper-polyaniline nano-composite. Biosensors & bioelectronics, 77, 505-511
Open this publication in new window or tab >>Creatinine and urea biosensors based on a novel ammonium ion-selective copper-polyaniline nano-composite
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2016 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 77, p. 505-511Article in journal (Refereed) Published
Abstract [en]

The use of a novel ammonium ion-specific copper-polyaniline nano-composite as transducer for hydrolase-based biosensors is proposed. In this work, a combination of creatinine deaminase and urease has been chosen as a model system to demonstrate the construction of urea and creatinine biosensors to illustrate the principle. Immobilisation of enzymes was shown to be a crucial step in the development of the biosensors; the use of glycerol and lactitol as stabilisers resulted in a significant improvement, especially in the case of the creatinine, of the operational stability of the biosensors (from few hours to at least 3 days). The developed biosensors exhibited high selectivity towards creatinine and urea. The sensitivity was found to be 85±3.4 mA M−1 cm−2 for the creatinine biosensor and 112±3.36 mA M−1 cm−2 for the urea biosensor, with apparent Michaelis–Menten constants (KM,app), obtained from the creatinine and urea calibration curves, of 0.163 mM for creatinine deaminase and 0.139 mM for urease, respectively. The biosensors responded linearly over the concentration range 1–125 µM, with a limit of detection of 0.5 µM and a response time of 15 s.

The performance of the biosensors in a real sample matrix, serum, was evaluated and a good correlation with standard spectrophotometric clinical laboratory techniques was found.

Place, publisher, year, edition, pages
Elsevier, 2016
Keywords
Creatinine; Urea; Amperometric ammonium detection; Copper polyanilinecomposite
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:liu:diva-123681 (URN)10.1016/j.bios.2015.10.009 (DOI)000366766900073 ()26457736 (PubMedID)
Projects
SMARTCABCERSENS
Funder
EU, FP7, Seventh Framework Programme, PIRSES-GA-2012-318053
Note

Funding agencies: SMARTCANCERSENS

Available from: 2016-01-08 Created: 2016-01-08 Last updated: 2017-12-01Bibliographically approved
Bagheryan, Z., Raoof, J.-B., Golabi, M., Turner, A. & Beni, V. (2016). Diazonium-based impedimetric aptasensor for the rapid label-free detection of Salmonella typhimurium in food sample. Biosensors & bioelectronics, 80, 566-573
Open this publication in new window or tab >>Diazonium-based impedimetric aptasensor for the rapid label-free detection of Salmonella typhimurium in food sample
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2016 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 80, p. 566-573Article in journal (Refereed) Published
Abstract [en]

Fast and accurate detection of microorganisms is of key importance in clinical analysis and in food and water quality monitoring. Salmonella typhimurium is responsible for about a third of all cases of food borne diseases and consequently, its fast detection is of great importance for ensuring the safety of foodstuffs. We report the development of a label-free impedimetric aptamer-based biosensor for S. typhimurium detection. The aptamer biosensor was fabricated by grafting a diazonium-supporting layer onto screen printed carbon electrodes (SPEs), via electrochemical or chemical approaches, followed by chemical immobilisation of aminated-aptamer. FTIR-ATR, contact angle and electrochemical measurements were used to monitor the fabrication process. Results showed that electrochemical immobilisation of the diazonium-grafting layer allowed the formation of a denser aptamer layer, which resulted in higher sensitivity. The developed aptamer-biosensor responded linearly, on a logarithm scale, over the concentration range 1 x 10(1) to 1 x 10(8) CFU mL(-1), with a limit of quantification (LOQ) of 1 x 10(1) CFU mL(-1) and a limit of detection (LOD) of 6 CFU mL(-1). Selectivity studies showed that the aptamer biosensor could discriminate S. typhimurium from 6 other model bacteria strains. Finally, recovery studies demonstrated its suitability for the detection of S. typhimurium in spiked (1 x 10(2), 1 x 10(4) and 1 x 10(6) CFU mL(-1)) apple juice samples. (C) 2016 Elsevier B.V. All rights reserved.

Place, publisher, year, edition, pages
ELSEVIER ADVANCED TECHNOLOGY, 2016
Keywords
Diazonium grafting; Aptamer; S. typhimurium; Label-free detection; Electrochemical impedance spectroscopy; Food analysis
National Category
Biological Sciences
Identifiers
urn:nbn:se:liu:diva-127249 (URN)10.1016/j.bios.2016.02.024 (DOI)000372558500079 ()26894987 (PubMedID)
Note

Funding Agencies|Vetenskapsradet (Pathoscreen project; Swedish Research Link) [D0675001]; Ministry of Science Research and Technology of Iran

Available from: 2016-04-20 Created: 2016-04-19 Last updated: 2018-03-26Bibliographically approved
Zhybak, M. T., Vagin, M. Y., Beni, V., Liu, X., Dempsey, E., Turner, A. P. F. & Korpan, Y. I. (2016). Direct detection of ammonium ion by means of oxygen electrocatalysis at a copper-polyaniline composite on a screen-printed electrode.. Microchimica Acta, 183(6), 1981-1987
Open this publication in new window or tab >>Direct detection of ammonium ion by means of oxygen electrocatalysis at a copper-polyaniline composite on a screen-printed electrode.
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2016 (English)In: Microchimica Acta, ISSN 0026-3672, E-ISSN 1436-5073, Vol. 183, no 6, p. 1981-1987Article in journal (Refereed) Published
Abstract [en]

A novel electrocatalytic material for oxygen reduction, based on polyaniline in combinationwith copper, was developed and utilised for the direct voltammetric quantification of ammonium ions. Consecutive electrode modification by electrodeposited copper, a Nafion membrane and electropolymerised polyaniline resulted in an electrocatalytic composite material which the retained conductivity at neutral pH. Ammonia complex formation with Cu (I) caused the appearance of oxygen electrocatalysis, which was observed as an increase in cathodic current. This Faradaic phenomenon offered the advantage of direct voltammetric detection and was utilised for ammonium electroanalysis. The developed quantification protocol was applied for ammonium assay in human serum and compared with the routine approach for clinical analysis.

Keywords
Conductive polymer, Nanocomposite, Amperometry, Electrochemical impedance spectroscopy, SEM, EDX, XPS, Serum analysis
National Category
Other Basic Medicine
Identifiers
urn:nbn:se:liu:diva-128192 (URN)10.1007/s00604-016-1834-3 (DOI)000376600300021 ()
Funder
EU, FP7, Seventh Framework Programme, PIRSES
Available from: 2016-05-20 Created: 2016-05-20 Last updated: 2018-03-23
Sheikhzadeh, E., Chamsaz, M., Turner, A., Jager, E. & Beni, V. (2016). Label-free impedimetric biosensor for Salmonella Typhimurium detection based on poly [pyrrole-co-3-carboxyl-pyrrole] copolymer supported aptamer. Biosensors & bioelectronics, 80, 194-200
Open this publication in new window or tab >>Label-free impedimetric biosensor for Salmonella Typhimurium detection based on poly [pyrrole-co-3-carboxyl-pyrrole] copolymer supported aptamer
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2016 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 80, p. 194-200Article in journal (Refereed) Published
Abstract [en]

The Gram-negative bacterium, Salmonella Typhimurium (S. Typhimurium) is a food borne pathogen responsible for numerous hospitalisations and deaths all over the world. Conventional detection methods for pathogens are time consuming and labour-intensive. Hence, there is considerable interest in faster and simpler detection methods. Polypyrrole-based polymers, due to their intrinsic chemical and electrical properties, have been demonstrated to be valuable candidates for the fabrication of chemo/biosensors and functional surfaces. Similarly aptamers have been shown to be good alternatives to antibodies in the development of affinity biosensors. In this study, we report on the combination of Poly [pyrrole-co-3-carboxyl-pyrrole] copolymer and aptamer for the development of a label-less electrochemical biosensor suitable for the detection of S. Typhimurium. Impedimetric measurements were facilitated by the effect of the aptamer/target interaction on the intrinsic conjugation of the poly [pyrrole-co-3-carboxyl-pyrrole] copolymer and subsequently on its electrical properties. The aptasensor detected S. Typhimurium in the concentration range 10(2)-10(8) CFU mL(-1) with high selectivity over other model pathogens and with a limit of quantification (LOQ) of 100 CFU mL(-1) and a limit of detection (LOD) of 3 CFU mL(-1). The suitability of the aptasensor for real sample detection was demonstrated via recovery studies performed in spiked apple juice samples. We envisage this to be a viable approach for the inexpensive and rapid detection of pathogens in food, and possibly in other environmental samples. (C) 2016 Elsevier B.V. All rights reserved.

Place, publisher, year, edition, pages
ELSEVIER ADVANCED TECHNOLOGY, 2016
Keywords
S. Typhimurium; Aptamer; Electrochemical impedance spectroscopy; Poly [pyrrole-co-3-carboxyl-pyrrole]; Label-free detection
National Category
Biological Sciences
Identifiers
urn:nbn:se:liu:diva-127248 (URN)10.1016/j.bios.2016.01.057 (DOI)000372558500029 ()26836649 (PubMedID)
Note

Funding Agencies|Ministry of Science Research and Technology of Iran; Research Council of Ferdowsi University of Mashhad [3/29828]; Linkoping University, Vetenskapsradet [D0675001]; Swedish Research Council [VR-2014-3079]

Available from: 2016-04-22 Created: 2016-04-19 Last updated: 2018-03-21
Mak, W. C., Beni, V. & Turner, A. (2016). Lateral-flow technology: from visual to instrumental.. TrAC. Trends in analytical chemistry, 79(SI), 297-305
Open this publication in new window or tab >>Lateral-flow technology: from visual to instrumental.
2016 (English)In: TrAC. Trends in analytical chemistry, ISSN 0165-9936, E-ISSN 1879-3142, Vol. 79, no SI, p. 297-305Article in journal (Refereed) Published
Abstract [en]

Lateral-flow tests were first launched commercially in 1984, as a simple urine-based pregnancy test for home use. The simplicity of the visual readout delivered by the basic lateral-flow format proved to be a very popular. However, the recent apparently unstoppable trend towards portable and wearable technology is driving the lateral-flow strip towards an industrial interface that will enable it to interface with big data and expert systems, and where ready transmission of data is essential. In this review, we chart the inevitable evolution of the visually-read lateral-flow strip to more advanced instrumented versions and consider the future of this very flexible approach to delivering simple affinity assays. We examine recent labelling strategies, the relative merits of optical and electrochemical transducers and explore the evolution of recognition elements that are now being incorporated into these systems.

Place, publisher, year, edition, pages
Elsevier, 2016
Keywords
Lateral-flow immunoassay; immunochromatography; paper-based diagnostics; visually-read assays; mobile phone diagnostics; cell phone assays; electrochemical immunoassay; gold nanoparticles; printed electronics; nucleic acid tests
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:liu:diva-123687 (URN)10.1016/j.trac.2015.10.017 (DOI)000376703900030 ()
Available from: 2016-01-08 Created: 2016-01-08 Last updated: 2017-12-01
Kor, K., Turner, A., Zarei, K., Atabati, M., Beni, V. & Mak, W. C. (2016). Structurally responsive oligonucleotide-based single-probe lateral-flow test for detection of miRNA-21 mimics. Analytical and Bioanalytical Chemistry, 408(5), 1475-1485
Open this publication in new window or tab >>Structurally responsive oligonucleotide-based single-probe lateral-flow test for detection of miRNA-21 mimics
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2016 (English)In: Analytical and Bioanalytical Chemistry, ISSN 1618-2642, E-ISSN 1618-2650, Vol. 408, no 5, p. 1475-1485Article in journal (Refereed) Published
Abstract [en]

A single-probe strip test for the rapid and sensitive detection of miRNA-21 mimics is reported herein. Highly specific structurally responsive bi-functional, thiol and biotin, DNA/LNA oligonucleotide probes (molecular beacons-MB) were designed and conjugated with gold nanoparticles (AuNPs) (i.e. biotin-MB-AuNPs). The proposed design had the ability to modulate the accessibility of the biotin group as a function of the presence of a miRNA target allowing the interaction of the boilable with the streptavidin test zone only in the presence of the miRNA-21 mimics. For quantitative evaluation, images of the strip tests were recorded using a flatbed scanner (Epson Perfection V370 Photo). The colour intensities of the test zones of the strip tests were analysed with the ImageJ software (Scion Corp., USA) and quantified as a function of pixel intensity. The response of the strip test was linear over the range 0.5 to 20 nM miRNA-21 (limit of detection of 115 pM) and showed good reproducibility (intra and inter CVs below 8 %); furthermore, the assay was shown to be highly selective, discriminating other interference miRNAs mimics (e.g. miRNA-221 and miRNA-205). Finally, the proposed strip test was used for detection of miRNA-21 mimics in spiked serum samples, demonstrating its potential for point-of-care clinical applications. Main advantages of the single-probe strip test design are its versatility, simplicity and robustness, which can be easily extended to other miRNA targets by tuning the sequence of the single probe. Furthermore, the use of the structurally responsive single probe is particularly relevant in the case of short-length targets, such as miRNA, whereas a conventional sandwich approach might require a careful control of assay conditions such as hybridization temperature and salt concentration

Place, publisher, year, edition, pages
Springer Berlin/Heidelberg, 2016
Keywords
miRNA-21, molecular beacon (MB), structurally responsive, lateral-flow strip tests
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:liu:diva-123675 (URN)10.1007/s00216-015-9250-9 (DOI)000368815200018 ()26700447 (PubMedID)
Note

Funding agencies: Ministry of Science, Research and Technology of Iran [214140]

Available from: 2016-01-07 Created: 2016-01-07 Last updated: 2017-12-01Bibliographically approved
Torrens, M., Ortiz, M., Turner, A., Beni, V. & OSullivan, C. K. (2015). Amperometric detection of Francisella tularensis genomic sequence on Zn-mediated diazonium modified substrates. Electrochemistry communications, 53
Open this publication in new window or tab >>Amperometric detection of Francisella tularensis genomic sequence on Zn-mediated diazonium modified substrates
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2015 (English)In: Electrochemistry communications, ISSN 1388-2481, E-ISSN 1873-1902, Vol. 53Article in journal (Refereed) Published
Abstract [en]

A simple, rapid and potentiostat-free method for grafting of close to monolayers of diazonium salts to anchor DNA probes for application in highly sensitive electrochemical genosensing is presented. Zn-mediated grafting is demonstrated to produce a highly organised surface facilitating enhanced sensitivity and a notably reduced detection limit as compared to electrochemical grafting. (C) 2015 Elsevier B.V. All rights reserved.

Place, publisher, year, edition, pages
Elsevier, 2015
Keywords
Diazonium salt; Surface grafting; Genosensor; Amperometry
National Category
Biological Sciences
Identifiers
urn:nbn:se:liu:diva-118250 (URN)10.1016/j.elecom.2015.02.003 (DOI)000353852100002 ()
Note

Funding Agencies|Commission of the European Communities (RTD programme) [2010-SEC-261810]; Generalitat de Catalunya

Available from: 2015-05-22 Created: 2015-05-22 Last updated: 2017-12-04
Kor, K., Turner, A., Zarei, K., Atabati, M., Beni, V. & Mak, M. (2015). Combining structure responsive probes and lateral-flow test; a simple and rapid approach miRNA-21 detection. In: 2nd International Congress on Biosensors – BiosensorTR2015, 10-12 June 2015, Izmir, Turkey.: . Paper presented at 2nd International Congress on Biosensors – BiosensorTR2015, 10-12 June 2015, Izmir, Turkey..
Open this publication in new window or tab >>Combining structure responsive probes and lateral-flow test; a simple and rapid approach miRNA-21 detection
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2015 (English)In: 2nd International Congress on Biosensors – BiosensorTR2015, 10-12 June 2015, Izmir, Turkey., 2015Conference paper, Poster (with or without abstract) (Refereed)
National Category
Biological Sciences
Identifiers
urn:nbn:se:liu:diva-118103 (URN)
Conference
2nd International Congress on Biosensors – BiosensorTR2015, 10-12 June 2015, Izmir, Turkey.
Available from: 2015-05-21 Created: 2015-05-21 Last updated: 2015-05-28
Sodzel, D., Khranovskyy, V., Beni, V., Turner, A. P., Viter, R., Eriksson, M. O., . . . Yakimova, R. (2015). Control of hydrogen peroxide and glucose via UV and Visible Photoluminescence of ZnO nanoparticles.. Microchimica Acta, 182(9-10), 1819-1826
Open this publication in new window or tab >>Control of hydrogen peroxide and glucose via UV and Visible Photoluminescence of ZnO nanoparticles.
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2015 (English)In: Microchimica Acta, ISSN 0026-3672, E-ISSN 1436-5073, Vol. 182, no 9-10, p. 1819-1826Article in journal (Refereed) Published
Abstract [en]

We report on an indirect optical method for the determination of glucose via the detection of hydrogen peroxide (H2O2) that is generated during the glucose oxidase (GOx) catalyzed oxidation of glucose. It is based on the finding that the ultraviolet (~374 nm) and visible (~525 nm) photoluminescence of pristine zinc oxide (ZnO) nanoparticles strongly depends on the concentration of H2O2 in water solution. Photoluminescence is quenched by up to 90 % at a 100 mM level of H2O2. The sensor constructed by immobilizing GOx on ZnO nanoparticles enabled glucose to be continuously monitored in the 10 mM to 130 mM concentration range, and the limit of detection is 10 mM. This enzymatic sensing scheme is supposed to be applicable to monitoring glucose in the food, beverage and fermentation industries. It has a wide scope in that it may be extended to numerous other substrate or enzyme activity assays based on the formation of H2O2, and of assays based on the consumption of H2O2 by peroxidases.

Place, publisher, year, edition, pages
Springer Netherlands, 2015
National Category
Materials Chemistry
Identifiers
urn:nbn:se:liu:diva-118305 (URN)10.1007/s00604-015-1493-9 (DOI)000356449700030 ()
Available from: 2015-05-26 Created: 2015-05-26 Last updated: 2017-12-04
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0001-6889-0351

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