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Phopase, Jaywant
Publications (10 of 15) Show all publications
Phopase, J. (2019). Comment on "Short peptide analogs as alternatives to collagen in pro-regenerative corneal implants" by Jangamreddy JR et al. [Letter to the editor]. Acta Biomaterialia, 97, 691-691
Open this publication in new window or tab >>Comment on "Short peptide analogs as alternatives to collagen in pro-regenerative corneal implants" by Jangamreddy JR et al.
2019 (English)In: Acta Biomaterialia, ISSN 1742-7061, E-ISSN 1878-7568, Vol. 97, p. 691-691Article in journal, Letter (Other academic) Published
Abstract [en]

n/a

Place, publisher, year, edition, pages
Elsevier, 2019
National Category
Biomaterials Science
Identifiers
urn:nbn:se:liu:diva-162349 (URN)10.1016/j.actbio.2019.06.004 (DOI)000495470300055 ()31176844 (PubMedID)2-s2.0-85073164566 (Scopus ID)
Available from: 2019-11-28 Created: 2019-11-28 Last updated: 2020-03-31Bibliographically approved
Che, C., Vagin, M., Ail, U., Gueskine, V., Phopase, J., Brooke, R., . . . Crispin, X. (2019). Twinning Lignosulfonate with a Conducting Polymer via Counter-Ion Exchange for Large-Scale Electrical Storage. Advanced Sustainable Systems, 3(9), Article ID 1900039.
Open this publication in new window or tab >>Twinning Lignosulfonate with a Conducting Polymer via Counter-Ion Exchange for Large-Scale Electrical Storage
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2019 (English)In: Advanced Sustainable Systems, ISSN 2366-7486, Vol. 3, no 9, article id 1900039Article in journal (Refereed) Published
Abstract [en]

Abstract Lignosulfonate (LS) is a large-scale surplus product of the forest and paper industries, and has primarily been utilized as a low-cost plasticizer in making concrete for the construction industry. LS is an anionic redox-active polyelectrolyte and is a promising candidate to boost the charge capacity of the positive electrode (positrode) in redox-supercapacitors. Here, the physical-chemical investigation of how this biopolymer incorporates into the conducting polymer PEDOT matrix, of the positrode, by means of counter-ion exchange is reported. Upon successful incorporation, an optimal access to redox moieties is achieved, which provides a 63% increase of the resulting stored electrical charge by reversible redox interconversion. The effects of pH, ionic strength, and concentrations, of included components, on the polymer?polymer interactions are optimized to exploit the biopolymer-associated redox currents. Further, the explored LS-conducting polymer incorporation strategy, via aqueous synthesis, is evaluated in an up-scaling effort toward large-scale electrical energy storage technology. By using an up-scaled production protocol, integration of the biopolymer within the conducting polymer matrix by counter-ion exchange is confirmed and the PEDOT-LS synthesized through optimized strategy reaches an improved charge capacity of 44.6 mAh g?1.

Place, publisher, year, edition, pages
John Wiley & Sons, 2019
Keywords
charge storage, conducting polymers, ion-exchange, lignin
National Category
Electrical Engineering, Electronic Engineering, Information Engineering
Identifiers
urn:nbn:se:liu:diva-161646 (URN)10.1002/adsu.201900039 (DOI)000486210400005 ()2-s2.0-85072220289 (Scopus ID)
Available from: 2019-11-05 Created: 2019-11-05 Last updated: 2019-11-11Bibliographically approved
Alarcon, E. I., Vulesevic, B., Argawal, A., Ross, A., Bejjani, P., Podrebarac, J., . . . Griffith, M. (2016). Coloured cornea replacements with anti-infective properties: expanding the safe use of silver nanoparticles in regenerative medicine.. Nanoscale, 8(12), 6484-6489
Open this publication in new window or tab >>Coloured cornea replacements with anti-infective properties: expanding the safe use of silver nanoparticles in regenerative medicine.
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2016 (English)In: Nanoscale, ISSN 2040-3364, E-ISSN 2040-3372, Vol. 8, no 12, p. 6484-6489Article in journal (Refereed) Published
Abstract [en]

Despite the broad anti-microbial and anti-inflammatory properties of silver nanoparticles (AgNPs), their use in bioengineered corneal replacements or bandage contact lenses has been hindered due to their intense yellow coloration. In this communication, we report the development of a new strategy to pre-stabilize and incorporate AgNPs with different colours into collagen matrices for fabrication of corneal implants and lenses, and assessed their in vitro and in vivo activity.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2016
National Category
Biomaterials Science
Identifiers
urn:nbn:se:liu:diva-126612 (URN)10.1039/c6nr01339b (DOI)000372851500033 ()26949000 (PubMedID)
Note

Funding agencies: Natural Sciences and Engineering Research Council [342107, RGPIN-2015-06325]; Swedish Research Council [521-2012-5706, 621-2012-4286]; University of Ottawa; Alexander Graham Bell/Canada Graduate (CGS-M/NSERC) Scholarship; Ontario Graduate Scholarships (OG

Available from: 2016-03-31 Created: 2016-03-31 Last updated: 2017-11-30Bibliographically approved
Ravichandran, R., Islam, M. M., Alarcon, E. I., Samanta, A., Wang, S., Lundström, P., . . . Phopase, J. (2016). Functionalised type-I collagen as a hydrogel building block for bio-orthogonal tissue engineering applications. Journal of materials chemistry. B, 4(2), 318-326
Open this publication in new window or tab >>Functionalised type-I collagen as a hydrogel building block for bio-orthogonal tissue engineering applications
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2016 (English)In: Journal of materials chemistry. B, ISSN 2050-750X, E-ISSN 2050-7518, Vol. 4, no 2, p. 318-326Article in journal (Refereed) Published
Abstract [en]

In this study, we derivatized type I collagen without altering its triple helical conformation to allow for facile hydrogel formation via the Michael addition of thiols to methacrylates without the addition of other crosslinking agents. This method provides the flexibility needed for the fabrication of injectable hydrogels or pre-fabricated implantable scaffolds, using the same components by tuning the modulus from Pa to kPa. Enzymatic degradability of the hydrogels can also be easily fine-tuned by variation of the ratio and the type of the crosslinking component. The structural morphology reveals a lamellar structure mimicking native collagen fibrils. The versatility of this material is demonstrated by its use as a pre-fabricated substrate for culturing human corneal epithelial cells and as an injectable hydrogel for 3-D encapsulation of cardiac progenitor cells.

Place, publisher, year, edition, pages
ROYAL SOC CHEMISTRY, 2016
National Category
Physical Sciences Chemical Sciences Clinical Medicine
Identifiers
urn:nbn:se:liu:diva-124491 (URN)10.1039/c5tb02035b (DOI)000367335200016 ()
Note

Funding Agencies|Swedish Research Council [621-2012-4286, 521-2012-5706]; NSERC; UOHI

Available from: 2016-02-02 Created: 2016-02-01 Last updated: 2017-11-30
Poblete, H., Agarwal, A., Thomas, S. S., Bohne, C., Ravichandran, R., Phopase, J., . . . Alarcon, E. I. (2016). New Insights into Peptide-Silver Nanoparticle Interaction: Deciphering the Role of Cysteine and Lysine in the Peptide Sequence. Langmuir, 32(1), 265-273
Open this publication in new window or tab >>New Insights into Peptide-Silver Nanoparticle Interaction: Deciphering the Role of Cysteine and Lysine in the Peptide Sequence
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2016 (English)In: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 32, no 1, p. 265-273Article in journal (Refereed) Published
Abstract [en]

We studied the interaction of four new pentapeptides with spherical silver nanoparticles. Our findings indicate that the combination of the thiol in Cys and amines in Lys/Arg residues is critical to providing stable protection for the silver surface. Molecular simulation reveals the atomic scale interactions that underlie the observed stabilizing effect of these peptides, while yielding qualitative agreement with experiment for ranking the affinity of the four pentapeptides for the silver surface.

Place, publisher, year, edition, pages
AMER CHEMICAL SOC, 2016
National Category
Physical Sciences
Identifiers
urn:nbn:se:liu:diva-125155 (URN)10.1021/acs.langmuir.5b03601 (DOI)000368321700031 ()26675437 (PubMedID)
Note

Funding Agencies|NSERC Discovery (EIA); University of Ottawa Heart Institute (EIA); Kansas Bioscience Authority funds; Kansas State University Global Food Systems Innovation Program [383GFS]; NSF [CNS-1006860, EPS-1006860, EPS-0919443]; National Science Foundation [ACI-1053575]; NSERC Discovery Grant [RGPIN-121389-2012]; Swedish Research Council [621-2012-4286]; [FONDECYT 3140288]

Available from: 2016-02-15 Created: 2016-02-15 Last updated: 2017-11-30
Ron-Doitch, S., Sawodny, B., Kuehbacher, A., Nordling David, M. M., Samanta, A., Phopase, J., . . . Rupp, S. (2016). Reduced cytotoxicity and enhanced bioactivity of cationic antimicrobial peptides liposomes in cell cultures and 3D epidermis model against HSV. Journal of Controlled Release, 229, 163-171
Open this publication in new window or tab >>Reduced cytotoxicity and enhanced bioactivity of cationic antimicrobial peptides liposomes in cell cultures and 3D epidermis model against HSV
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2016 (English)In: Journal of Controlled Release, ISSN 0168-3659, E-ISSN 1873-4995, Vol. 229, p. 163-171Article in journal (Refereed) Published
Abstract [en]

Cationic antimicrobial peptides (AMPs) are part of the innate immunity, and act against a wide variety of pathogenic microorganisms by perturbation of the microorganisms plasma membrane. Although attractive for clinical applications, these agents suffer from limited stability and activity in vivo, as well as non-specific interaction with host biological membranes, leading to cytotoxic adverse effects. We hypothesized that encapsulation of AMPs within liposomes could result in reduced cytotoxicity, and with enhanced stability as well as bioactivity against herpes simplex virus 1 (HSV-1). We formulated nano-sized liposomal formulations of LL-37 and indolicidin, and their physicochemical properties, cellular uptake, in vitro cytotoxicity and antiviral efficacy have been determined. Lower cytotoxicity of LL-37 liposomes was found in comparison to indolicidin liposomes attributed to the superior physicochemical properties, and to the different degree of interaction with the liposomal membrane. The disc-like shaped LL-37 liposomes (106.8 +/- 10.1 nm, shelf-life stability of N1 year) were taken up more rapidly and to a significantly higher extent than the free peptide by human keratinocyte cell line (HaCaT), remained intact within the cells, followed by release of the active peptide within the cytoplasm and migration of the vesicles lipids to the plasma membrane. LL-37 liposomes were found significantly less toxic than both the free agent and liposomal indolicidin. In the new 3D epidermis model (immortalized primary keratinocytes) liposomal LL-37 treatment (N20 mu M), but not free LL-37, efficiently protected the epidermis, inhibiting HSV-1 infection. This positive antiviral effect was obtained with no cytotoxicity even at very high concentrations (400 mu M). Thus, the antiviral activity of encapsulated LL-37 was significantly improved, expanding its therapeutic window. Liposomal LL-37 appears to be a promising delivery system for HSV therapy. (C) 2016 Elsevier B.V. All rights reserved.

Place, publisher, year, edition, pages
ELSEVIER SCIENCE BV, 2016
Keywords
Drug delivery system; Liposomes; Antimicrobial peptides; LL-37; Indolicidin; Peptide delivery; Disc-like liposomes; HSV-1; 3D epidermis model
National Category
Physical Sciences Clinical Medicine
Identifiers
urn:nbn:se:liu:diva-128724 (URN)10.1016/j.jconrel.2016.03.025 (DOI)000375218900015 ()27012977 (PubMedID)
Note

Funding Agencies|Fraunhofer Institute (Germany) - Hebrew University of Jerusalem (Israel); Sir Zelman Cowen Universities Fund

Available from: 2016-06-01 Created: 2016-05-30 Last updated: 2017-05-01
Islam, M. M., Ravichandran, R., Olsen, D., Kozak Ljunggren, M., Fagerholm, P., Lee, C.-J., . . . Phopase, J. (2016). Self-assembled collagen-like-peptide implants as alternatives to human donor corneal transplantation. RSC Advances, 6(61), 55745-55749
Open this publication in new window or tab >>Self-assembled collagen-like-peptide implants as alternatives to human donor corneal transplantation
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2016 (English)In: RSC Advances, ISSN 2046-2069, E-ISSN 2046-2069, Vol. 6, no 61, p. 55745-55749Article in journal (Refereed) Published
Abstract [en]

Extracellular matrix proteins like collagen promote regeneration as implants in clinical studies. However, collagens are large and unwieldy proteins, making small functional peptide analogs potentially ideal substitutes. Self-assembling collagen-like-peptides conjugated with PEG-maleimide were assembled into hydrogels. When tested pre-clinically as corneal implants in mini-pigs, they promoted cell and nerve regeneration, forming neo-corneas structurally and functionally similar to natural corneas.

Place, publisher, year, edition, pages
ROYAL SOC CHEMISTRY, 2016
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:liu:diva-130324 (URN)10.1039/c6ra08895c (DOI)000378275400008 ()
Note

Funding Agencies|Vinnova Indo-Sweden grant [2013-04645]; Integrative Regenerative Medicine Centre, Linkoping University (LiU); Region Ostergotland; Swedish Research Council grant [621-2012-4286]

Available from: 2016-07-29 Created: 2016-07-28 Last updated: 2017-11-28
Ravichandran, R., Griffith, M. & Phopase, J. (2014). Applications of self-assembling peptide scaffolds in regenerative medicine: the way to the clinic. Journal of materials chemistry. B, 2(48), 8466-8478
Open this publication in new window or tab >>Applications of self-assembling peptide scaffolds in regenerative medicine: the way to the clinic
2014 (English)In: Journal of materials chemistry. B, ISSN 2050-750X, E-ISSN 2050-7518, Vol. 2, no 48, p. 8466-8478Article in journal (Refereed) Published
Abstract [en]

Peptides that self-assemble into well-defined nanofibrous networks provide a prominent alternative to traditional biomaterials for fabricating scaffolds for use in regenerative medicine and other biomedical applications. Such scaffolds can be generated by decorating a peptide backbone with other bioactives such as cell specific adhesion peptides, growth factors and enzyme cleavable sequences. They can be designed to mimic the three-dimensional (3D) structural features of native ECM and can therefore also provide insight into the ECM-cell interactions needed for development of scaffolds that can serve as regeneration templates for specific target tissues or organs. This review highlights the potential application of self-assembling peptides in regenerative medicine.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2014
National Category
Physical Sciences Clinical Medicine
Identifiers
urn:nbn:se:liu:diva-113073 (URN)10.1039/c4tb01095g (DOI)000345529400002 ()
Note

Funding Agencies|Swedish Research Council, Sweden [2012-42315-94008-81]

Available from: 2015-01-09 Created: 2015-01-08 Last updated: 2017-12-05
Lee, C.-J., Buznyk, O., Kuffova, L., Rajendran, V., Forrester, J. V., Phopase, J., . . . Griffith, M. (2014). Cathelicidin LL-37 and HSV-1 Corneal Infection: Peptide Versus Gene Therapy. Translational Vision Science & Technology, 3(3), 1-14
Open this publication in new window or tab >>Cathelicidin LL-37 and HSV-1 Corneal Infection: Peptide Versus Gene Therapy
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2014 (English)In: Translational Vision Science & Technology, ISSN 2164-2591, Vol. 3, no 3, p. 1-14Article in journal (Refereed) Published
Abstract [en]

Purpose: To evaluate the potential utility of collagen-based corneal implants with anti?Herpes Simplex Virus (HSV)-1 activity achieved through sustained release of LL-37, from incorporated nanoparticles, as compared with cell-based delivery from model human corneal epithelial cells (HCECs) transfected to produce endogenous LL-37. Methods: We tested the ability of collagen-phosphorylcholine implants to tolerate the adverse microenvironment of herpetic murine corneas. Then, we investigated the efficacy of LL-37 peptides delivered through nanoparticles incorporated within the corneal implants to block HSV-1 viral activity. In addition, LL-37 complementary DNA (cDNA) was transferred into HCECs to confer viral resistance, and their response to HSV-1 infection was examined. Results: Our implants remained in herpetic murine corneas 7 days longer than allografts. LL-37 released from the implants blocked HSV-1 infection of HCECs by interfering with viral binding. However, in pre-infected HCECs, LL-37 delayed but could not prevent viral spreading nor clear viruses from the infected cells. HCECs transfected with the LL-37 expressed and secreted the peptide. Secreted LL-37 inhibited viral binding in vitro but was insufficient to protect cells completely from HSV-1 infection. Nevertheless, secreted LL-37 reduced both the incidence of plaque formation and plaque size. Conclusion: LL-37 released from composite nanoparticle-hydrogel corneal implants and HCEC-produced peptide, both showed anti?HSV-1 activity by blocking binding. However, while both slowed down virus spread, neither was able on its own to completely inhibit the viruses. Translational Relevance: LL-37 releasing hydrogels may have potential utility as corneal substitutes for grafting in HSV-1 infected corneas, possibly in combination with LL-37 producing therapeutic cells.

Place, publisher, year, edition, pages
Association for Research in Vision and Ophthalmology, 2014
Keywords
cornea, HSV-1, antiviral peptides; nanoparticles, gene transfer
National Category
Ophthalmology
Identifiers
urn:nbn:se:liu:diva-111486 (URN)10.1167/tvst.3.3.4 (DOI)000209813600004 ()24932432 (PubMedID)
Available from: 2014-10-19 Created: 2014-10-19 Last updated: 2019-02-11Bibliographically approved
Vignoni, M., de Alwis Weerasekera, H., Simpson, M. J., Phopase, J., Mah, T.-F., Griffith, M., . . . Scaiano, J. (2014). LL37 peptide@silver nanoparticles: combining the best of the two worlds for skin infection control. Nanoscale, 6(11), 5725-5728
Open this publication in new window or tab >>LL37 peptide@silver nanoparticles: combining the best of the two worlds for skin infection control
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2014 (English)In: Nanoscale, ISSN 2040-3364, E-ISSN 2040-3372, Vol. 6, no 11, p. 5725-5728Article in journal (Refereed) Published
Abstract [en]

Capping silver nanoparticles with LL37 peptide eradicates the anti-proliferative effect of silver on primary skin cells, but retains the bactericidal properties of silver nanoparticles with activities comparable to silver nitrate or silver sulfadiazine. In addition, LL37 capped silver nanoparticles have anti-biofilm formation activity.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2014
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-108794 (URN)10.1039/c4nr01284d (DOI)000336883000027 ()24789474 (PubMedID)
Available from: 2014-07-07 Created: 2014-07-06 Last updated: 2017-12-05Bibliographically approved
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