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Phopase, Jaywant
Publications (10 of 13) Show all publications
Alarcon, E. I., Vulesevic, B., Argawal, A., Ross, A., Bejjani, P., Podrebarac, J., . . . Griffith, M. (2016). Coloured cornea replacements with anti-infective properties: expanding the safe use of silver nanoparticles in regenerative medicine.. Nanoscale, 8(12), 6484-6489
Open this publication in new window or tab >>Coloured cornea replacements with anti-infective properties: expanding the safe use of silver nanoparticles in regenerative medicine.
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2016 (English)In: Nanoscale, ISSN 2040-3364, E-ISSN 2040-3372, Vol. 8, no 12, p. 6484-6489Article in journal (Refereed) Published
Abstract [en]

Despite the broad anti-microbial and anti-inflammatory properties of silver nanoparticles (AgNPs), their use in bioengineered corneal replacements or bandage contact lenses has been hindered due to their intense yellow coloration. In this communication, we report the development of a new strategy to pre-stabilize and incorporate AgNPs with different colours into collagen matrices for fabrication of corneal implants and lenses, and assessed their in vitro and in vivo activity.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2016
National Category
Biomaterials Science
Identifiers
urn:nbn:se:liu:diva-126612 (URN)10.1039/c6nr01339b (DOI)000372851500033 ()26949000 (PubMedID)
Note

Funding agencies: Natural Sciences and Engineering Research Council [342107, RGPIN-2015-06325]; Swedish Research Council [521-2012-5706, 621-2012-4286]; University of Ottawa; Alexander Graham Bell/Canada Graduate (CGS-M/NSERC) Scholarship; Ontario Graduate Scholarships (OG

Available from: 2016-03-31 Created: 2016-03-31 Last updated: 2017-11-30Bibliographically approved
Ravichandran, R., Islam, M. M., Alarcon, E. I., Samanta, A., Wang, S., Lundström, P., . . . Phopase, J. (2016). Functionalised type-I collagen as a hydrogel building block for bio-orthogonal tissue engineering applications. Journal of materials chemistry. B, 4(2), 318-326
Open this publication in new window or tab >>Functionalised type-I collagen as a hydrogel building block for bio-orthogonal tissue engineering applications
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2016 (English)In: Journal of materials chemistry. B, ISSN 2050-750X, E-ISSN 2050-7518, Vol. 4, no 2, p. 318-326Article in journal (Refereed) Published
Abstract [en]

In this study, we derivatized type I collagen without altering its triple helical conformation to allow for facile hydrogel formation via the Michael addition of thiols to methacrylates without the addition of other crosslinking agents. This method provides the flexibility needed for the fabrication of injectable hydrogels or pre-fabricated implantable scaffolds, using the same components by tuning the modulus from Pa to kPa. Enzymatic degradability of the hydrogels can also be easily fine-tuned by variation of the ratio and the type of the crosslinking component. The structural morphology reveals a lamellar structure mimicking native collagen fibrils. The versatility of this material is demonstrated by its use as a pre-fabricated substrate for culturing human corneal epithelial cells and as an injectable hydrogel for 3-D encapsulation of cardiac progenitor cells.

Place, publisher, year, edition, pages
ROYAL SOC CHEMISTRY, 2016
National Category
Physical Sciences Chemical Sciences Clinical Medicine
Identifiers
urn:nbn:se:liu:diva-124491 (URN)10.1039/c5tb02035b (DOI)000367335200016 ()
Note

Funding Agencies|Swedish Research Council [621-2012-4286, 521-2012-5706]; NSERC; UOHI

Available from: 2016-02-02 Created: 2016-02-01 Last updated: 2017-11-30
Poblete, H., Agarwal, A., Thomas, S. S., Bohne, C., Ravichandran, R., Phopase, J., . . . Alarcon, E. I. (2016). New Insights into Peptide-Silver Nanoparticle Interaction: Deciphering the Role of Cysteine and Lysine in the Peptide Sequence. Langmuir, 32(1), 265-273
Open this publication in new window or tab >>New Insights into Peptide-Silver Nanoparticle Interaction: Deciphering the Role of Cysteine and Lysine in the Peptide Sequence
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2016 (English)In: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 32, no 1, p. 265-273Article in journal (Refereed) Published
Abstract [en]

We studied the interaction of four new pentapeptides with spherical silver nanoparticles. Our findings indicate that the combination of the thiol in Cys and amines in Lys/Arg residues is critical to providing stable protection for the silver surface. Molecular simulation reveals the atomic scale interactions that underlie the observed stabilizing effect of these peptides, while yielding qualitative agreement with experiment for ranking the affinity of the four pentapeptides for the silver surface.

Place, publisher, year, edition, pages
AMER CHEMICAL SOC, 2016
National Category
Physical Sciences
Identifiers
urn:nbn:se:liu:diva-125155 (URN)10.1021/acs.langmuir.5b03601 (DOI)000368321700031 ()26675437 (PubMedID)
Note

Funding Agencies|NSERC Discovery (EIA); University of Ottawa Heart Institute (EIA); Kansas Bioscience Authority funds; Kansas State University Global Food Systems Innovation Program [383GFS]; NSF [CNS-1006860, EPS-1006860, EPS-0919443]; National Science Foundation [ACI-1053575]; NSERC Discovery Grant [RGPIN-121389-2012]; Swedish Research Council [621-2012-4286]; [FONDECYT 3140288]

Available from: 2016-02-15 Created: 2016-02-15 Last updated: 2017-11-30
Ron-Doitch, S., Sawodny, B., Kuehbacher, A., Nordling David, M. M., Samanta, A., Phopase, J., . . . Rupp, S. (2016). Reduced cytotoxicity and enhanced bioactivity of cationic antimicrobial peptides liposomes in cell cultures and 3D epidermis model against HSV. Journal of Controlled Release, 229, 163-171
Open this publication in new window or tab >>Reduced cytotoxicity and enhanced bioactivity of cationic antimicrobial peptides liposomes in cell cultures and 3D epidermis model against HSV
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2016 (English)In: Journal of Controlled Release, ISSN 0168-3659, E-ISSN 1873-4995, Vol. 229, p. 163-171Article in journal (Refereed) Published
Abstract [en]

Cationic antimicrobial peptides (AMPs) are part of the innate immunity, and act against a wide variety of pathogenic microorganisms by perturbation of the microorganisms plasma membrane. Although attractive for clinical applications, these agents suffer from limited stability and activity in vivo, as well as non-specific interaction with host biological membranes, leading to cytotoxic adverse effects. We hypothesized that encapsulation of AMPs within liposomes could result in reduced cytotoxicity, and with enhanced stability as well as bioactivity against herpes simplex virus 1 (HSV-1). We formulated nano-sized liposomal formulations of LL-37 and indolicidin, and their physicochemical properties, cellular uptake, in vitro cytotoxicity and antiviral efficacy have been determined. Lower cytotoxicity of LL-37 liposomes was found in comparison to indolicidin liposomes attributed to the superior physicochemical properties, and to the different degree of interaction with the liposomal membrane. The disc-like shaped LL-37 liposomes (106.8 +/- 10.1 nm, shelf-life stability of N1 year) were taken up more rapidly and to a significantly higher extent than the free peptide by human keratinocyte cell line (HaCaT), remained intact within the cells, followed by release of the active peptide within the cytoplasm and migration of the vesicles lipids to the plasma membrane. LL-37 liposomes were found significantly less toxic than both the free agent and liposomal indolicidin. In the new 3D epidermis model (immortalized primary keratinocytes) liposomal LL-37 treatment (N20 mu M), but not free LL-37, efficiently protected the epidermis, inhibiting HSV-1 infection. This positive antiviral effect was obtained with no cytotoxicity even at very high concentrations (400 mu M). Thus, the antiviral activity of encapsulated LL-37 was significantly improved, expanding its therapeutic window. Liposomal LL-37 appears to be a promising delivery system for HSV therapy. (C) 2016 Elsevier B.V. All rights reserved.

Place, publisher, year, edition, pages
ELSEVIER SCIENCE BV, 2016
Keywords
Drug delivery system; Liposomes; Antimicrobial peptides; LL-37; Indolicidin; Peptide delivery; Disc-like liposomes; HSV-1; 3D epidermis model
National Category
Physical Sciences Clinical Medicine
Identifiers
urn:nbn:se:liu:diva-128724 (URN)10.1016/j.jconrel.2016.03.025 (DOI)000375218900015 ()27012977 (PubMedID)
Note

Funding Agencies|Fraunhofer Institute (Germany) - Hebrew University of Jerusalem (Israel); Sir Zelman Cowen Universities Fund

Available from: 2016-06-01 Created: 2016-05-30 Last updated: 2017-05-01
Islam, M. M., Ravichandran, R., Olsen, D., Kozak Ljunggren, M., Fagerholm, P., Lee, C.-J., . . . Phopase, J. (2016). Self-assembled collagen-like-peptide implants as alternatives to human donor corneal transplantation. RSC Advances, 6(61), 55745-55749
Open this publication in new window or tab >>Self-assembled collagen-like-peptide implants as alternatives to human donor corneal transplantation
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2016 (English)In: RSC Advances, ISSN 2046-2069, E-ISSN 2046-2069, Vol. 6, no 61, p. 55745-55749Article in journal (Refereed) Published
Abstract [en]

Extracellular matrix proteins like collagen promote regeneration as implants in clinical studies. However, collagens are large and unwieldy proteins, making small functional peptide analogs potentially ideal substitutes. Self-assembling collagen-like-peptides conjugated with PEG-maleimide were assembled into hydrogels. When tested pre-clinically as corneal implants in mini-pigs, they promoted cell and nerve regeneration, forming neo-corneas structurally and functionally similar to natural corneas.

Place, publisher, year, edition, pages
ROYAL SOC CHEMISTRY, 2016
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:liu:diva-130324 (URN)10.1039/c6ra08895c (DOI)000378275400008 ()
Note

Funding Agencies|Vinnova Indo-Sweden grant [2013-04645]; Integrative Regenerative Medicine Centre, Linkoping University (LiU); Region Ostergotland; Swedish Research Council grant [621-2012-4286]

Available from: 2016-07-29 Created: 2016-07-28 Last updated: 2017-11-28
Ravichandran, R., Griffith, M. & Phopase, J. (2014). Applications of self-assembling peptide scaffolds in regenerative medicine: the way to the clinic. Journal of materials chemistry. B, 2(48), 8466-8478
Open this publication in new window or tab >>Applications of self-assembling peptide scaffolds in regenerative medicine: the way to the clinic
2014 (English)In: Journal of materials chemistry. B, ISSN 2050-750X, E-ISSN 2050-7518, Vol. 2, no 48, p. 8466-8478Article in journal (Refereed) Published
Abstract [en]

Peptides that self-assemble into well-defined nanofibrous networks provide a prominent alternative to traditional biomaterials for fabricating scaffolds for use in regenerative medicine and other biomedical applications. Such scaffolds can be generated by decorating a peptide backbone with other bioactives such as cell specific adhesion peptides, growth factors and enzyme cleavable sequences. They can be designed to mimic the three-dimensional (3D) structural features of native ECM and can therefore also provide insight into the ECM-cell interactions needed for development of scaffolds that can serve as regeneration templates for specific target tissues or organs. This review highlights the potential application of self-assembling peptides in regenerative medicine.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2014
National Category
Physical Sciences Clinical Medicine
Identifiers
urn:nbn:se:liu:diva-113073 (URN)10.1039/c4tb01095g (DOI)000345529400002 ()
Note

Funding Agencies|Swedish Research Council, Sweden [2012-42315-94008-81]

Available from: 2015-01-09 Created: 2015-01-08 Last updated: 2017-12-05
Lee, C.-J., Buznyk, O., Kuffova, L., Rajendran, V., Forrester, J. V., Phopase, J., . . . Griffith, M. (2014). Cathelicidin LL-37 and HSV-1 Corneal Infection: Peptide Versus Gene Therapy. Translational Vision Science & Technology, 3(3), 1-14
Open this publication in new window or tab >>Cathelicidin LL-37 and HSV-1 Corneal Infection: Peptide Versus Gene Therapy
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2014 (English)In: Translational Vision Science & Technology, ISSN 2164-2591, Vol. 3, no 3, p. 1-14Article in journal (Refereed) Published
Abstract [en]

Purpose: To evaluate the potential utility of collagen-based corneal implants with anti?Herpes Simplex Virus (HSV)-1 activity achieved through sustained release of LL-37, from incorporated nanoparticles, as compared with cell-based delivery from model human corneal epithelial cells (HCECs) transfected to produce endogenous LL-37. Methods: We tested the ability of collagen-phosphorylcholine implants to tolerate the adverse microenvironment of herpetic murine corneas. Then, we investigated the efficacy of LL-37 peptides delivered through nanoparticles incorporated within the corneal implants to block HSV-1 viral activity. In addition, LL-37 complementary DNA (cDNA) was transferred into HCECs to confer viral resistance, and their response to HSV-1 infection was examined. Results: Our implants remained in herpetic murine corneas 7 days longer than allografts. LL-37 released from the implants blocked HSV-1 infection of HCECs by interfering with viral binding. However, in pre-infected HCECs, LL-37 delayed but could not prevent viral spreading nor clear viruses from the infected cells. HCECs transfected with the LL-37 expressed and secreted the peptide. Secreted LL-37 inhibited viral binding in vitro but was insufficient to protect cells completely from HSV-1 infection. Nevertheless, secreted LL-37 reduced both the incidence of plaque formation and plaque size. Conclusion: LL-37 released from composite nanoparticle-hydrogel corneal implants and HCEC-produced peptide, both showed anti?HSV-1 activity by blocking binding. However, while both slowed down virus spread, neither was able on its own to completely inhibit the viruses. Translational Relevance: LL-37 releasing hydrogels may have potential utility as corneal substitutes for grafting in HSV-1 infected corneas, possibly in combination with LL-37 producing therapeutic cells.

Place, publisher, year, edition, pages
Association for Research in Vision and Ophthalmology, 2014
Keywords
cornea, HSV-1, antiviral peptides; nanoparticles, gene transfer
National Category
Ophthalmology
Identifiers
urn:nbn:se:liu:diva-111486 (URN)10.1167/tvst.3.3.4 (DOI)000209813600004 ()24932432 (PubMedID)
Available from: 2014-10-19 Created: 2014-10-19 Last updated: 2019-02-11Bibliographically approved
Vignoni, M., de Alwis Weerasekera, H., Simpson, M. J., Phopase, J., Mah, T.-F., Griffith, M., . . . Scaiano, J. (2014). LL37 peptide@silver nanoparticles: combining the best of the two worlds for skin infection control. Nanoscale, 6(11), 5725-5728
Open this publication in new window or tab >>LL37 peptide@silver nanoparticles: combining the best of the two worlds for skin infection control
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2014 (English)In: Nanoscale, ISSN 2040-3364, E-ISSN 2040-3372, Vol. 6, no 11, p. 5725-5728Article in journal (Refereed) Published
Abstract [en]

Capping silver nanoparticles with LL37 peptide eradicates the anti-proliferative effect of silver on primary skin cells, but retains the bactericidal properties of silver nanoparticles with activities comparable to silver nitrate or silver sulfadiazine. In addition, LL37 capped silver nanoparticles have anti-biofilm formation activity.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2014
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-108794 (URN)10.1039/c4nr01284d (DOI)000336883000027 ()24789474 (PubMedID)
Available from: 2014-07-07 Created: 2014-07-06 Last updated: 2017-12-05Bibliographically approved
Rabong, C., Schuster, C., Liptaj, T., Pronayova, N., Delchev, V. B., Jordis, U. & Phopase, J. (2014). NXO beta structure mimicry: an ultrashort turn/hairpin mimic that folds in water. RSC Advances, 4(41), 21351-21360
Open this publication in new window or tab >>NXO beta structure mimicry: an ultrashort turn/hairpin mimic that folds in water
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2014 (English)In: RSC Advances, ISSN 2046-2069, E-ISSN 2046-2069, Vol. 4, no 41, p. 21351-21360Article in journal (Refereed) Published
Abstract [en]

We report the first application of NXO-pseudopeptides for beta-turn mimicry. Incorporating the proline-derived NProO peptidomimetic building block, a minimal tetrapeptide beta-hairpin mimic has been designed, synthesized and its solution structure elucidated. Emulating a natural proline-glycine beta-turn, evidence from NMR, molecular modeling and CD suggests the formation of two rapidly interconverting hairpin folds in water, methanol and dimethyl-sulfoxide at room temperature, displaying the proline nitrogen amide bond in either cis or trans arrangement. The NProO-modified hairpin features peptidic backbone dihedrals phi, Psi characteristic of natural proline-containing turns composed of alpha-amino acids only. Taken together, the observed folding behavior and inherently high designability render the NProO motif a building block for beta-structure elaboration in aqueous medium.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2014
National Category
Engineering and Technology
Identifiers
urn:nbn:se:liu:diva-108819 (URN)10.1039/c4ra01210k (DOI)000336837100026 ()
Available from: 2014-07-07 Created: 2014-07-06 Last updated: 2017-12-05
Wickham, A. M., Islam, M. M., Mondal, D., Phopase, J., Sadhu, V., Tamás, É., . . . Griffith, M. (2014). Polycaprolactone–thiophene-conjugated carbon nanotube meshes as scaffolds for cardiac progenitor cells. Journal of Biomedical Materials Research. Part B - Applied biomaterials, 102(7), 1553-1561
Open this publication in new window or tab >>Polycaprolactone–thiophene-conjugated carbon nanotube meshes as scaffolds for cardiac progenitor cells
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2014 (English)In: Journal of Biomedical Materials Research. Part B - Applied biomaterials, ISSN 1552-4973, E-ISSN 1552-4981, Vol. 102, no 7, p. 1553-1561Article in journal (Refereed) Published
Abstract [en]

The myocardium is unable to regenerate itself after infarct, resulting in scarring and thinning of the heart wall. Our objective was to develop a patch to buttress and bypass the scarred area, while allowing regeneration by incorporated cardiac stem/progenitor cells (CPCs). Polycaprolactone (PCL) was fabricated as both sheets by solvent casting, and fibrous meshes by electrospinning, as potential patches, to determine the role of topology in proliferation and phenotypic changes to the CPCs. Thiophene-conjugated carbon nanotubes (T-CNTs) were incorporated to enhance the mechanical strength. We showed that freshly isolated CPCs from murine hearts neither attached nor spread on the PCL sheets, both with and without T-CNT. As electrospun meshes, however, both PCL and PCL/T-CNT supported CPC adhesion, proliferation, and differentiation. The incorporation of T-CNT into PCL resulted in a significant increase in mechanical strength but no morphological changes to the meshes. In turn, proliferation, but not differentiation, of CPCs into cardiomyocytes was enhanced in T-CNT containing meshes. We have shown that changing the topology of PCL, a known hydrophobic material, dramatically altered its properties, in this case, allowing CPCs to survive and differentiate. With further development, PCL/T-CNT meshes or similar patches may become a viable strategy to aid restoration of the postmyocardial infarction myocardium.

Place, publisher, year, edition, pages
John Wiley & Sons, 2014
Keywords
topology, carbon nanotubes, polycaprolactone, cardiac progenitor cells, electrospun meshes
National Category
Clinical Medicine Basic Medicine Physical Sciences
Identifiers
urn:nbn:se:liu:diva-111488 (URN)10.1002/jbm.b.33136 (DOI)000342963000020 ()24664884 (PubMedID)
Available from: 2014-10-19 Created: 2014-10-19 Last updated: 2018-01-11Bibliographically approved
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