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Islam, Mohammad Mirazul
Alternative names
Publications (10 of 12) Show all publications
Patra, H. K., Azharuddin, M., Islam, M. M., Papapavlou, G., Deb, S., Osterrieth, J., . . . Slater, N. K. H. (2019). Rational Nanotoolbox with Theranostic Potential for Medicated Pro-Regenerative Corneal Implants. Advanced Functional Materials, Article ID 1903760.
Open this publication in new window or tab >>Rational Nanotoolbox with Theranostic Potential for Medicated Pro-Regenerative Corneal Implants
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2019 (English)In: Advanced Functional Materials, ISSN 1616-301X, E-ISSN 1616-3028, article id 1903760Article in journal (Refereed) Epub ahead of print
Abstract [en]

Cornea diseases are a leading cause of blindness and the disease burden is exacerbated by the increasing shortage around the world for cadaveric donor corneas. Despite the advances in the field of regenerative medicine, successful transplantation of laboratory‐made artificial corneas is not fully realized in clinical practice. The causes of failure of such artificial corneal implants are multifactorial and include latent infections from viruses and other microbes, enzyme overexpression, implant degradation, extrusion or delayed epithelial regeneration. Therefore, there is an urgent unmet need for developing customized corneal implants to suit the host environment and counter the effects of inflammation or infection, which are able to track early signs of implant failure in situ. This work reports a nanotoolbox comprising tools for protection from infection, promotion of regeneration, and noninvasive monitoring of the in situ corneal environment. These nanosystems can be incorporated within pro‐regenerative biosynthetic implants, transforming them into theranostic devices, which are able to respond to biological changes following implantation.

Place, publisher, year, edition, pages
John Wiley & Sons, 2019
Keywords
herpes simplex virus type 1 (HSV-1), magnetic resonance imaging (MRI), premedicated cornea implants, pro-regeneration, theranostics
National Category
Microbiology in the medical area
Identifiers
urn:nbn:se:liu:diva-159097 (URN)10.1002/adfm.201903760 (DOI)000476281800001 ()2-s2.0-85069940064 (Scopus ID)
Note

Funding agencies: EU H2020 Marie Sklodowska-Curie Individual Fellowship [706694]; MIIC Strategic Postdoc Grant; MIIC Seed Grant at Linkoping University (LiU), Sweden

Available from: 2019-07-24 Created: 2019-07-24 Last updated: 2019-11-13Bibliographically approved
Islam, M. M., Buznyk, O., Reddy, J. C., Pasyechnikova, N., Alarcon, E. I., Hayes, S., . . . Griffith, M. (2018). Biomaterials-enabled cornea regeneration in patients at high risk for rejection of donor tissue transplantation. NPJ Regenerative medicine, 3, Article ID 2.
Open this publication in new window or tab >>Biomaterials-enabled cornea regeneration in patients at high risk for rejection of donor tissue transplantation
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2018 (English)In: NPJ Regenerative medicine, ISSN 2057-3995, Vol. 3, article id 2Article in journal (Refereed) Published
Abstract [en]

The severe worldwide shortage of donor organs, and severe pathologies placing patients at high risk for rejecting conventional cornea transplantation, have left many corneal blind patients untreated. Following successful pre-clinical evaluation in mini-pigs, we tested a biomaterials-enabled pro-regeneration strategy to restore corneal integrity in an open-label observational study of six patients. Cell-free corneal implants comprising recombinant human collagen and phosphorylcholine were grafted by anterior lamellar keratoplasty into corneas of unilaterally blind patients diagnosed at high-risk for rejecting donor allografts. They were followed-up for a mean of 24 months. Patients with acute disease (ulceration) were relieved of pain and discomfort within 1-2 weeks post-operation. Patients with scarred or ulcerated corneas from severe infection showed better vision improvement, followed by corneas with burns. Corneas with immune or degenerative conditions transplanted for symptom relief only showed no vision improvement overall. However, grafting promoted nerve regeneration as observed by improved touch sensitivity to near normal levels in all patients tested, even for those with little/no sensitivity before treatment. Overall, three out of six patients showed significant vision improvement. Others were sufficiently stabilized to allow follow-on surgery to restore vision. Grafting outcomes in mini-pig corneas were superior to those in human subjects, emphasizing that animal models are only predictive for patients with non-severely pathological corneas; however, for establishing parameters such as stable corneal tissue and nerve regeneration, our pig model is satisfactory. While further testing is merited, we have nevertheless shown that cell-free implants are potentially safe, efficacious options for treating high-risk patients.

National Category
Surgery
Identifiers
urn:nbn:se:liu:diva-152526 (URN)10.1038/s41536-017-0038-8 (DOI)29423280 (PubMedID)
Available from: 2019-03-28 Created: 2019-03-28 Last updated: 2019-03-28
Islam, M. M., Ravichandran, R., Olsen, D., Kozak Ljunggren, M., Fagerholm, P., Lee, C.-J., . . . Phopase, J. (2016). Self-assembled collagen-like-peptide implants as alternatives to human donor corneal transplantation. RSC Advances, 6(61), 55745-55749
Open this publication in new window or tab >>Self-assembled collagen-like-peptide implants as alternatives to human donor corneal transplantation
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2016 (English)In: RSC Advances, ISSN 2046-2069, E-ISSN 2046-2069, Vol. 6, no 61, p. 55745-55749Article in journal (Refereed) Published
Abstract [en]

Extracellular matrix proteins like collagen promote regeneration as implants in clinical studies. However, collagens are large and unwieldy proteins, making small functional peptide analogs potentially ideal substitutes. Self-assembling collagen-like-peptides conjugated with PEG-maleimide were assembled into hydrogels. When tested pre-clinically as corneal implants in mini-pigs, they promoted cell and nerve regeneration, forming neo-corneas structurally and functionally similar to natural corneas.

Place, publisher, year, edition, pages
ROYAL SOC CHEMISTRY, 2016
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:liu:diva-130324 (URN)10.1039/c6ra08895c (DOI)000378275400008 ()
Note

Funding Agencies|Vinnova Indo-Sweden grant [2013-04645]; Integrative Regenerative Medicine Centre, Linkoping University (LiU); Region Ostergotland; Swedish Research Council grant [621-2012-4286]

Available from: 2016-07-29 Created: 2016-07-28 Last updated: 2017-11-28
Buznyk, O., Pasyechnikova, N., Islam, M. M., Iakymenko, S., Fagerholm, P. & Griffith, M. (2015). Bioengineered Corneas Grafted as Alternatives to Human Donor Corneas in Three High-Risk Patients. Clinical and Translational Science, 8(5), 558-562
Open this publication in new window or tab >>Bioengineered Corneas Grafted as Alternatives to Human Donor Corneas in Three High-Risk Patients
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2015 (English)In: Clinical and Translational Science, ISSN 1752-8054, E-ISSN 1752-8062, Vol. 8, no 5, p. 558-562Article in journal (Refereed) Published
Abstract [en]

Corneas with severe pathologies have a high risk of rejection when conventionally grafted with human donor tissues. In this early observational study, we grafted bioengineered corneal implants made from recombinant human collagen and synthetic phosphorylcholine polymer into three patients for whom donor cornea transplantation carried a high risk of transplant failure. These patients suffered from corneal ulcers and recurrent erosions preoperatively. The implants provided relief from pain and discomfort, restored corneal integrity by promoting endogenous regeneration of corneal tissues, and improved vision in two of three patients. Such implants could in the future be alternatives to donor corneas for high-risk patients, and therefore, merits further testing in a clinical trial.

Place, publisher, year, edition, pages
WILEY-BLACKWELL, 2015
Keywords
collagen; epithelium; grafting; patients; remodeling; transplantation
National Category
Other Basic Medicine
Identifiers
urn:nbn:se:liu:diva-122789 (URN)10.1111/cts.12293 (DOI)000363652100024 ()25996570 (PubMedID)
Note

Funding Agencies|Swedish Research Council (EU Nanomedicine project "I-CARE") [521-2012-5706]; Integrative Regenerative Medicine Centre, Linkoping, Sweden; Swedish Institute fellowship

Available from: 2015-11-23 Created: 2015-11-23 Last updated: 2018-01-10
Islam, M. M., Cėpla, V., He, C., Edin, J., Rakickas, T., Kobuch, K., . . . Griffith, M. (2015). Functional fabrication of recombinant human collagen–phosphorylcholine hydrogels for regenerative medicine applications. Acta Biomaterialia, 12, 70-80
Open this publication in new window or tab >>Functional fabrication of recombinant human collagen–phosphorylcholine hydrogels for regenerative medicine applications
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2015 (English)In: Acta Biomaterialia, ISSN 1742-7061, E-ISSN 1878-7568, Vol. 12, p. 70-80Article in journal (Refereed) Published
Abstract [en]

The implant-host interface is a critical element in guiding tissue or organ regeneration. We previously developed hydrogels comprising interpenetrating networks of recombinant human collagen type III and 2-methacryloyloxyethyl phosphorylcholine (RHCIII-MPC) as substitutes of the corneal extracellular matrix that promote endogenous regeneration of corneal tissue. To render them functional for clinical application, we have now optimized their composition and thereby enhanced their mechanical properties. We have demonstrated that such optimized RHCIII-MPC hydrogels are suitable for precision femtosecond laser cutting to produce complementing implants and host surgical beds for subsequent tissue welding. This avoids the tissue damage and inflammation associated with manual surgical techniques, thereby leading to more efficient healing. Although we previously demonstrated in clinical testing that RHCIII-based implants stimulated cornea regeneration in patients, the rate of epithelial cell coverage of the implants needs improvement, e.g. modification of the implant surface. We now show that our 500 μm thick RHCIII-MPC constructs comprising over 85% water, are suitable for microcontact printing with fibronectin. The resulting fibronectin micropatterns promote cell adhesion, as compared to the bare RHCIII-MPC hydrogel. Interestingly, a pattern of 30 μm wide fibronectin stripes enhanced cell attachment and showed highest mitotic rates, an effect that potentially can be utilized for faster integration of the implant. We have therefore shown that laboratory-produced mimics of naturally occurring collagen and phospholipids can be fabricated into robust hydrogels that can be laser profiled and patterned to enhance their potential function as artificial substitutes of donor human corneas.

Place, publisher, year, edition, pages
Elsevier, 2015
Keywords
Hydrogel, Cornea, Collagen, Fibronectin, Laser ablation, Surface modification
National Category
Basic Medicine Clinical Medicine
Identifiers
urn:nbn:se:liu:diva-111782 (URN)10.1016/j.actbio.2014.10.035 (DOI)000348686100008 ()
Note

We thank Dr. Chyan-Jang Lee for establishing the GFP-HCEC cell line used for this study, and Ms. Kimberley Merrett for assistance in characterization of the hydrogels. We also thank Dr. Sadhana Kulkani and David Priest, University of Ottawa Eye Institue, for assistance with the laser cutting study; and Dr. Joanne M. Hackett (currently at Cambridge University Health Partners) for assistance with preliminary cell culture/biocompatibility studies during optimization of the RHCIII-MPC hydrogels. We thank Johannes Junger and Michael Baumann, MLase AG, for help with the UV crosslinking, and the Medical Devices Bureau, Health Canada, for use of the SEM system. We gratefully acknowledge funding from an NSERC-CIHR Canada Collaborative Health Research Project grant (M.G.) and subsequent funding for an EU Nanomedicine ERAnet project "I-CARE" to M.G., R.V. and MLase AG, through the Swedish Research Council, Research Council of Lithuania and VDI Germany, respectively.

Available from: 2014-11-03 Created: 2014-11-03 Last updated: 2018-01-11Bibliographically approved
Alarcon, E. I., Udekwu, K. I., Noel, C. W., Gagnon, L.-P. B. -., Taylor, P. K., Vulesevic, B., . . . Griffith, M. (2015). Safety and efficacy of composite collagen-silver nanoparticle hydrogels as tissue engineering scaffolds. Nanoscale, 7(44), 18789-18798
Open this publication in new window or tab >>Safety and efficacy of composite collagen-silver nanoparticle hydrogels as tissue engineering scaffolds
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2015 (English)In: Nanoscale, ISSN 2040-3364, E-ISSN 2040-3372, Vol. 7, no 44, p. 18789-18798Article in journal (Refereed) Published
Abstract [en]

The increasing number of multidrug resistant bacteria has revitalized interest in seeking alternative sources for controlling bacterial infection. Silver nanoparticles (AgNPs), are amongst the most promising candidates due to their wide microbial spectrum of action. In this work, we report on the safety and efficacy of the incorporation of collagen coated AgNPs into collagen hydrogels for tissue engineering. The resulting hybrid materials at [AgNPs] less than0.4 mu M retained the mechanical properties and biocompatibility for primary human skin fibroblasts and keratinocytes of collagen hydrogels; they also displayed remarkable anti-infective properties against S. aureus, S. epidermidis, E. coli and P. aeruginosa at considerably lower concentrations than silver nitrate. Further, subcutaneous implants of materials containing 0.2 mu M AgNPs in mice showed a reduction in the levels of IL-6 and other inflammation markers (CCL24, sTNFR-2, and TIMP1). Finally, an analysis of silver contents in implanted mice showed that silver accumulation primarily occurred within the tissue surrounding the implant.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2015
National Category
Physical Sciences Clinical Medicine
Identifiers
urn:nbn:se:liu:diva-123536 (URN)10.1039/c5nr03826j (DOI)000364824000048 ()26507748 (PubMedID)
Note

Funding Agencies|Natural Sciences and Engineering Research Council of Canada; NSERC/CIHR Canada; Swedish Research Council [621-2010-5189]; AFA Forsakring; University of Ottawa Heart Institute Startup grant

Available from: 2015-12-21 Created: 2015-12-21 Last updated: 2017-12-01Bibliographically approved
Lee, C.-J., Buznyk, O., Kuffova, L., Rajendran, V., Forrester, J. V., Phopase, J., . . . Griffith, M. (2014). Cathelicidin LL-37 and HSV-1 Corneal Infection: Peptide Versus Gene Therapy. Translational Vision Science & Technology, 3(3), 1-14
Open this publication in new window or tab >>Cathelicidin LL-37 and HSV-1 Corneal Infection: Peptide Versus Gene Therapy
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2014 (English)In: Translational Vision Science & Technology, ISSN 2164-2591, Vol. 3, no 3, p. 1-14Article in journal (Refereed) Published
Abstract [en]

Purpose: To evaluate the potential utility of collagen-based corneal implants with anti?Herpes Simplex Virus (HSV)-1 activity achieved through sustained release of LL-37, from incorporated nanoparticles, as compared with cell-based delivery from model human corneal epithelial cells (HCECs) transfected to produce endogenous LL-37. Methods: We tested the ability of collagen-phosphorylcholine implants to tolerate the adverse microenvironment of herpetic murine corneas. Then, we investigated the efficacy of LL-37 peptides delivered through nanoparticles incorporated within the corneal implants to block HSV-1 viral activity. In addition, LL-37 complementary DNA (cDNA) was transferred into HCECs to confer viral resistance, and their response to HSV-1 infection was examined. Results: Our implants remained in herpetic murine corneas 7 days longer than allografts. LL-37 released from the implants blocked HSV-1 infection of HCECs by interfering with viral binding. However, in pre-infected HCECs, LL-37 delayed but could not prevent viral spreading nor clear viruses from the infected cells. HCECs transfected with the LL-37 expressed and secreted the peptide. Secreted LL-37 inhibited viral binding in vitro but was insufficient to protect cells completely from HSV-1 infection. Nevertheless, secreted LL-37 reduced both the incidence of plaque formation and plaque size. Conclusion: LL-37 released from composite nanoparticle-hydrogel corneal implants and HCEC-produced peptide, both showed anti?HSV-1 activity by blocking binding. However, while both slowed down virus spread, neither was able on its own to completely inhibit the viruses. Translational Relevance: LL-37 releasing hydrogels may have potential utility as corneal substitutes for grafting in HSV-1 infected corneas, possibly in combination with LL-37 producing therapeutic cells.

Place, publisher, year, edition, pages
Association for Research in Vision and Ophthalmology, 2014
Keywords
cornea, HSV-1, antiviral peptides; nanoparticles, gene transfer
National Category
Ophthalmology
Identifiers
urn:nbn:se:liu:diva-111486 (URN)10.1167/tvst.3.3.4 (DOI)000209813600004 ()24932432 (PubMedID)
Available from: 2014-10-19 Created: 2014-10-19 Last updated: 2019-02-11Bibliographically approved
Wickham, A. M., Islam, M. M., Mondal, D., Phopase, J., Sadhu, V., Tamás, É., . . . Griffith, M. (2014). Polycaprolactone–thiophene-conjugated carbon nanotube meshes as scaffolds for cardiac progenitor cells. Journal of Biomedical Materials Research. Part B - Applied biomaterials, 102(7), 1553-1561
Open this publication in new window or tab >>Polycaprolactone–thiophene-conjugated carbon nanotube meshes as scaffolds for cardiac progenitor cells
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2014 (English)In: Journal of Biomedical Materials Research. Part B - Applied biomaterials, ISSN 1552-4973, E-ISSN 1552-4981, Vol. 102, no 7, p. 1553-1561Article in journal (Refereed) Published
Abstract [en]

The myocardium is unable to regenerate itself after infarct, resulting in scarring and thinning of the heart wall. Our objective was to develop a patch to buttress and bypass the scarred area, while allowing regeneration by incorporated cardiac stem/progenitor cells (CPCs). Polycaprolactone (PCL) was fabricated as both sheets by solvent casting, and fibrous meshes by electrospinning, as potential patches, to determine the role of topology in proliferation and phenotypic changes to the CPCs. Thiophene-conjugated carbon nanotubes (T-CNTs) were incorporated to enhance the mechanical strength. We showed that freshly isolated CPCs from murine hearts neither attached nor spread on the PCL sheets, both with and without T-CNT. As electrospun meshes, however, both PCL and PCL/T-CNT supported CPC adhesion, proliferation, and differentiation. The incorporation of T-CNT into PCL resulted in a significant increase in mechanical strength but no morphological changes to the meshes. In turn, proliferation, but not differentiation, of CPCs into cardiomyocytes was enhanced in T-CNT containing meshes. We have shown that changing the topology of PCL, a known hydrophobic material, dramatically altered its properties, in this case, allowing CPCs to survive and differentiate. With further development, PCL/T-CNT meshes or similar patches may become a viable strategy to aid restoration of the postmyocardial infarction myocardium.

Place, publisher, year, edition, pages
John Wiley & Sons, 2014
Keywords
topology, carbon nanotubes, polycaprolactone, cardiac progenitor cells, electrospun meshes
National Category
Clinical Medicine Basic Medicine Physical Sciences
Identifiers
urn:nbn:se:liu:diva-111488 (URN)10.1002/jbm.b.33136 (DOI)000342963000020 ()24664884 (PubMedID)
Available from: 2014-10-19 Created: 2014-10-19 Last updated: 2018-01-11Bibliographically approved
Koh, L. B., Islam, M. M., Mitra, D., Noel, C., Merett, K., Odorcic, S., . . . Griffith, M. (2013). Epoxy Cross-Linked Collagen and Collagen-Laminin Peptide Hydrogels as Corneal Substitutes. Journal of Functional Biomaterials, 4(3), 162-177
Open this publication in new window or tab >>Epoxy Cross-Linked Collagen and Collagen-Laminin Peptide Hydrogels as Corneal Substitutes
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2013 (English)In: Journal of Functional Biomaterials, ISSN 2079-4983, E-ISSN 2079-4983, Vol. 4, no 3, p. 162-177Article in journal (Refereed) Published
Abstract [en]

A bi-functional epoxy-based cross-linker, 1,4-Butanediol diglycidyl ether (BDDGE), was investigated in the fabrication of collagen based corneal substitutes. Two synthetic strategies were explored in the preparation of the cross-linked collagen scaffolds. The lysine residues of Type 1 porcine collagen were directly cross-linked using l,4-Butanediol diglycidyl ether (BDDGE) under basic conditions at pH 11. Alternatively, under conventional methodology, using both BDDGE and 1-Ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS) as cross-linkers, hydrogels were fabricated under acidic conditions. In this latter strategy, Cu(BF4)2·XH2O was used to catalyze the formation of secondary amine bonds. To date, we have demonstrated that both methods of chemical cross-linking improved the elasticity and tensile strength of the collagen implants. Differential scanning calorimetry and biocompatibility studies indicate comparable, and in some cases, enhanced properties compared to that of the EDC/NHS controls. In vitro studies showed that human corneal epithelial cells and neuronal progenitor cell lines proliferated on these hydrogels. In addition, improvement of cell proliferation on the surfaces of the materials was observed when neurite promoting laminin epitope, IKVAV, and adhesion peptide, YIGSR, were incorporated. However, the elasticity decreased with peptide incorporation and will require further optimization. Nevertheless, we have shown that epoxy cross-linkers should be further explored in the fabrication of collagen-based hydrogels, as alternatives to or in conjunction with carbodiimide cross-linkers.

Place, publisher, year, edition, pages
Basel, Switzerland: MDPI AG, 2013
Keywords
biomimetic materials; cross-linking; collagen; cornea; tissue engineering
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-98700 (URN)10.3390/jfb4030162 (DOI)
Available from: 2013-10-11 Created: 2013-10-11 Last updated: 2017-12-06Bibliographically approved
Islam, M. M., Griffith, M. & Merrett, K. (2013). Fabrication of a human recombinant collagen-based corneal substitute using carbodiimide chemistry. Methods in Molecular Biology, 1014, 157-164
Open this publication in new window or tab >>Fabrication of a human recombinant collagen-based corneal substitute using carbodiimide chemistry
2013 (English)In: Methods in Molecular Biology, ISSN 1064-3745, E-ISSN 1940-6029, Vol. 1014, p. 157-164Article in journal (Refereed) Published
Abstract [en]

Human recombinant collagen can be cross-linked with a variety of chemical cross-linking agents. Cross-linking methods can be tuned to confer collagen-based scaffolds with specific physical properties, improved antigenicity and thermal stability without impeding the ability of the material to integrate into the surrounding tissue and to promote regeneration. Here, we describe a method to cross-link human recombinant collagen using a water soluble carbodiimide. Carbodiimides are referred to as zero-length cross-linking agents as they are not incorporated into the final cross-link and thus pose minimal risk with respect to cytotoxicity. The resulting collagen-based scaffold possesses properties comparable to that of the human cornea and is thus suitable for use as a corneal substitute.

Place, publisher, year, edition, pages
Humana Press, 2013
Keywords
Biosynthetic corneal implant, Collagen, Carbodiimide, Collagen cross-linking, Collagen-based scaffold
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-98694 (URN)10.1007/978-1-62703-432-6_10 (DOI)23690011 (PubMedID)978-1-62703-431-9 (ISBN)
Note

ISBN: 978-1-62703-431-9

Available from: 2013-10-11 Created: 2013-10-11 Last updated: 2017-12-06Bibliographically approved
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