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Gelmi, Amy
Publications (7 of 7) Show all publications
Gelmi, A., Zhang, J., Cieslar-Pobuda, A., Ljunggren, M., Los, M., Rafat, M. & Jager, E. (2015). Electroactive polymer scaffolds for cardiac tissue engineering. In: Bar-Cohen (Ed.), Proc. SPIE 9430, Electroactive Polymer Actuators and Devices (EAPAD) 2015: . Paper presented at Electroactive Polymer Actuators and Devices (EAPAD) 2015 (pp. 94301T-1-94301T-7). SPIE - International Society for Optical Engineering, 9430
Open this publication in new window or tab >>Electroactive polymer scaffolds for cardiac tissue engineering
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2015 (English)In: Proc. SPIE 9430, Electroactive Polymer Actuators and Devices (EAPAD) 2015 / [ed] Bar-Cohen, SPIE - International Society for Optical Engineering, 2015, Vol. 9430, p. 94301T-1-94301T-7Conference paper, Published paper (Refereed)
Abstract [en]

By-pass surgery and heart transplantation are traditionally used to restore the heart’s functionality after a myocardial Infarction (MI or heart attack) that results in scar tissue formation and impaired cardiac function. However, both procedures are associated with serious post-surgical complications. Therefore, new strategies to help re-establish heart functionality are necessary. Tissue engineering and stem cell therapy are the promising approaches that are being explored for the treatment of MI. The stem cell niche is extremely important for the proliferation and differentiation of stem cells and tissue regeneration. For the introduction of stem cells into the host tissue an artificial carrier such as a scaffold is preferred as direct injection of stem cells has resulted in fast stem cell death. Such scaffold will provide the proper microenvironment that can be altered electronically to provide temporal stimulation to the cells. We have developed an electroactive polymer (EAP) scaffold for cardiac tissue engineering. The EAP scaffold mimics the extracellular matrix and provides a 3D microenvironment that can be easily tuned during fabrication, such as controllable fibre dimensions, alignment, and coating. In addition, the scaffold can provide electrical and electromechanical stimulation to the stem cells which are important external stimuli to stem cell differentiation. We tested the initial biocompatibility of these scaffolds using cardiac progenitor cells (CPCs), and continued onto more sensitive induced pluripotent stem cells (iPS). We present the fabrication and characterisation of these electroactive fibres as well as the response of increasingly sensitive cell types to the scaffolds.

Place, publisher, year, edition, pages
SPIE - International Society for Optical Engineering, 2015
Series
Proceedings of SPIE, ISSN 0277-786X ; 9430
National Category
Medical Materials
Identifiers
urn:nbn:se:liu:diva-118260 (URN)10.1117/12.2084165 (DOI)000355580900052 ()
Conference
Electroactive Polymer Actuators and Devices (EAPAD) 2015
Available from: 2015-05-22 Created: 2015-05-22 Last updated: 2020-09-08Bibliographically approved
Gelmi, A., Rafat, M. & Jager, E. (2014). Actuating electroactive scaffolds for cardiac tissue regeneration. In: : . Paper presented at EuroEAP 2014. Fourth international conference on Electromechanically Active Polymer (EAP) transducers and artificial muscles.
Open this publication in new window or tab >>Actuating electroactive scaffolds for cardiac tissue regeneration
2014 (English)Conference paper, Poster (with or without abstract) (Refereed)
National Category
Textile, Rubber and Polymeric Materials
Identifiers
urn:nbn:se:liu:diva-128242 (URN)
Conference
EuroEAP 2014. Fourth international conference on Electromechanically Active Polymer (EAP) transducers and artificial muscles
Available from: 2016-05-23 Created: 2016-05-23 Last updated: 2018-10-11
Gelmi, A., Kozak Ljunggren, M., Rafat, M. & Jager, E. (2014). Bioelectronic nanofibre scaffolds for tissue engineering and whole-cell biosensors. In: : . Paper presented at 24th Anniversary World Congress on Biosensors – Biosensors 2014. 24th Anniversary World Congress on Biosensors – Biosensors 2014, 27-30 May 2014, Melbourne, Australia..
Open this publication in new window or tab >>Bioelectronic nanofibre scaffolds for tissue engineering and whole-cell biosensors
2014 (English)Conference paper, Oral presentation only (Refereed)
National Category
Medical Engineering
Identifiers
urn:nbn:se:liu:diva-128245 (URN)
Conference
24th Anniversary World Congress on Biosensors – Biosensors 2014. 24th Anniversary World Congress on Biosensors – Biosensors 2014, 27-30 May 2014, Melbourne, Australia.
Available from: 2016-05-23 Created: 2016-05-23 Last updated: 2018-10-11
Gelmi, A., Kozak Ljunggren, M., Rafat, M. & Jager, E. (2014). Influence of conductive polymer doping on the viability of cardiac progenitor cells. Journal of materials chemistry. B, 2(24), 3860-3867
Open this publication in new window or tab >>Influence of conductive polymer doping on the viability of cardiac progenitor cells
2014 (English)In: Journal of materials chemistry. B, ISSN 2050-750X, E-ISSN 2050-7518, Vol. 2, no 24, p. 3860-3867Article in journal (Refereed) Published
Abstract [en]

Cardiac tissue engineering via the use of stem cells is the future for repairing impaired heart function that results from a myocardial infarction. Developing an optimised platform to support the stem cells is vital to realising this, and through utilising new smart materials such as conductive polymers we can provide a multi-pronged approach to supporting and stimulating the stem cells via engineered surface properties, electrical, and electromechanical stimulation. Here we present a fundamental study on the viability of cardiac progenitor cells on conductive polymer surfaces, focusing on the impact of surface properties such as roughness, surface energy, and surface chemistry with variation of the polymer dopant molecules. The conductive polymer materials were shown to provide a viable support for both endothelial and cardiac progenitor cells, while the surface energy and roughness were observed to influence viability for both progenitor cell types. Characterising the interaction between the cardiac progenitor cells and the conductive polymer surface is a critical step towards optimising these materials for cardiac tissue regeneration, and this study will advance the limited knowledge on biomaterial surface interactions with cardiac cells.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2014
National Category
Engineering and Technology
Identifiers
urn:nbn:se:liu:diva-108817 (URN)10.1039/c4tb00142g (DOI)000336853400015 ()
Available from: 2014-07-07 Created: 2014-07-06 Last updated: 2017-12-05Bibliographically approved
Gelmi, A., Kozak Ljunggren, M., Rafat, M. & Jager, E. (2014). Smart Electroactive Scaffolds for Cardiac Tissue Regeneration. In: : . Paper presented at NanoMed, International Conference on Nanotechnology in Medicine.
Open this publication in new window or tab >>Smart Electroactive Scaffolds for Cardiac Tissue Regeneration
2014 (English)Conference paper, Oral presentation only (Refereed)
National Category
Medical Engineering
Identifiers
urn:nbn:se:liu:diva-128243 (URN)
Conference
NanoMed, International Conference on Nanotechnology in Medicine
Available from: 2016-05-23 Created: 2016-05-23 Last updated: 2018-10-11
Gelmi, A., Higgins, M., Wallace, G., Rafat, M. & Jager, E. (2013). Electroactive Biomaterial Solutions for Tissue Engineering. In: : . Paper presented at Workshop Global Challenges - Opportunities for Nanotechnology, 15-18 April 2013, Venice, Italy.
Open this publication in new window or tab >>Electroactive Biomaterial Solutions for Tissue Engineering
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2013 (English)Conference paper, Poster (with or without abstract) (Other academic)
National Category
Biomaterials Science
Identifiers
urn:nbn:se:liu:diva-103838 (URN)
Conference
Workshop Global Challenges - Opportunities for Nanotechnology, 15-18 April 2013, Venice, Italy
Available from: 2014-01-29 Created: 2014-01-29 Last updated: 2014-10-08Bibliographically approved
Gelmi, A., Ljunggren, M., Rafat, M. & Jager, E. (2013). Electroactive scaffolds for cardiac tissue regeneration. In: : . Paper presented at EuroEAP 2013. Third international conference on Electromechanically Active Polymer (EAP) transducers and artificial muscles, June 25-26, Dübendorf (Zürich), Switzerland.
Open this publication in new window or tab >>Electroactive scaffolds for cardiac tissue regeneration
2013 (English)Conference paper, Poster (with or without abstract) (Other academic)
Abstract [en]

Myocardial Infarction (MI), commonly known as a heart attack, is the interruption of blood supply to a part of the heart, causing heart cells to die. In order to restore function by-pass surgery or ultimately heart transplantation is needed. However, due to the shortage of organ donors and complications associated with immune suppressive treatments, development of new strategies to help regenerate the injured heart is necessary. Stem cell therapy can be used to repair necrotic heart tissue and achieve myocardial regeneration. This research is focused on developing implantable electroactive fiber scaffolds that will increase the differentiation ratio of mesenchymal stem cells into cardiomyocytes and thus increase the formation of novel cardiac tissue to repair or replace the damaged cardiac tissue after MI. Composite nanofibrous scaffold of poly(dl-lactide-co-glycolide) (PLGA) have been coated with biodoped polypyrrole to create an electroactive fiber scaffold, with controllable fiber dimensions and alignment. The electrical properties of the polymers are an integral factor in creating these 'intelligent' 3-D materials; not only does the inherent conductivity provide a platform for electrical stimulation, but the ionic actuation of the polymer can also provide mechanical stimulation to the seeded cells. The biocompatibility of the polymer, PLGA scaffolds, and coated PLGA scaffolds has been investigated using primary cardiovascular progenitor cells.

National Category
Biomaterials Science
Identifiers
urn:nbn:se:liu:diva-100530 (URN)
Conference
EuroEAP 2013. Third international conference on Electromechanically Active Polymer (EAP) transducers and artificial muscles, June 25-26, Dübendorf (Zürich), Switzerland
Available from: 2013-11-08 Created: 2013-11-08 Last updated: 2014-10-08Bibliographically approved
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