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Ljunggren, Monika
Alternative names
Publications (10 of 11) Show all publications
Puckert, C., Gelmi, A., Kozak Ljunggren, M., Rafat, M. & Jager, E. (2016). Optimisation of conductive polymer biomaterials for cardiac progenitor cells. RSC Advances, 6(67), 62270-62277
Open this publication in new window or tab >>Optimisation of conductive polymer biomaterials for cardiac progenitor cells
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2016 (English)In: RSC Advances, ISSN 2046-2069, E-ISSN 2046-2069, Vol. 6, no 67, p. 62270-62277Article in journal (Refereed) Published
Abstract [en]

The characterisation of biomaterials for cardiac tissue engineering applications is vital for the development of effective treatments for the repair of cardiac function. New smart materials developed from conductive polymers can provide dynamic benefits in supporting and stimulating stem cells via controlled surface properties, electrical and electromechanical stimulation. In this study we investigate the control of surface properties of conductive polymers through a systematic approach to variable synthesis parameters, and how the resulting surface properties influence the viability of cardiac progenitor cells. A thorough analysis investigating the effect of electropolymerisation parameters, such as current density and growth, and reagent variation on physical properties provides a fundamental understanding of how to optimise conductive polymer biomaterials for cardiac progenitor cells.

Place, publisher, year, edition, pages
ROYAL SOC CHEMISTRY, 2016
National Category
Polymer Chemistry
Identifiers
urn:nbn:se:liu:diva-130451 (URN)10.1039/c6ra11682e (DOI)000379678200028 ()
Note

Funding Agencies|Linkoping University; IGEN (post-doc grant); COST-Action [MP1003]; Knut och Alice Wallenberg Commemorative Fund; European Research Agency

Available from: 2016-08-06 Created: 2016-08-05 Last updated: 2018-10-11
Islam, M. M., Ravichandran, R., Olsen, D., Kozak Ljunggren, M., Fagerholm, P., Lee, C.-J., . . . Phopase, J. (2016). Self-assembled collagen-like-peptide implants as alternatives to human donor corneal transplantation. RSC Advances, 6(61), 55745-55749
Open this publication in new window or tab >>Self-assembled collagen-like-peptide implants as alternatives to human donor corneal transplantation
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2016 (English)In: RSC Advances, ISSN 2046-2069, E-ISSN 2046-2069, Vol. 6, no 61, p. 55745-55749Article in journal (Refereed) Published
Abstract [en]

Extracellular matrix proteins like collagen promote regeneration as implants in clinical studies. However, collagens are large and unwieldy proteins, making small functional peptide analogs potentially ideal substitutes. Self-assembling collagen-like-peptides conjugated with PEG-maleimide were assembled into hydrogels. When tested pre-clinically as corneal implants in mini-pigs, they promoted cell and nerve regeneration, forming neo-corneas structurally and functionally similar to natural corneas.

Place, publisher, year, edition, pages
ROYAL SOC CHEMISTRY, 2016
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:liu:diva-130324 (URN)10.1039/c6ra08895c (DOI)000378275400008 ()
Note

Funding Agencies|Vinnova Indo-Sweden grant [2013-04645]; Integrative Regenerative Medicine Centre, Linkoping University (LiU); Region Ostergotland; Swedish Research Council grant [621-2012-4286]

Available from: 2016-07-29 Created: 2016-07-28 Last updated: 2017-11-28
Gelmi, A., Zhang, J., Cieslar-Pobuda, A., Ljunggren, M., Los, M., Rafat, M. & Jager, E. (2015). Electroactive polymer scaffolds for cardiac tissue engineering. In: Bar-Cohen (Ed.), Proc. SPIE 9430, Electroactive Polymer Actuators and Devices (EAPAD) 2015: . Paper presented at Electroactive Polymer Actuators and Devices (EAPAD) 2015 (pp. 94301T-1-94301T-7). SPIE - International Society for Optical Engineering, 9430
Open this publication in new window or tab >>Electroactive polymer scaffolds for cardiac tissue engineering
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2015 (English)In: Proc. SPIE 9430, Electroactive Polymer Actuators and Devices (EAPAD) 2015 / [ed] Bar-Cohen, SPIE - International Society for Optical Engineering, 2015, Vol. 9430, p. 94301T-1-94301T-7Conference paper, Published paper (Refereed)
Abstract [en]

By-pass surgery and heart transplantation are traditionally used to restore the heart’s functionality after a myocardial Infarction (MI or heart attack) that results in scar tissue formation and impaired cardiac function. However, both procedures are associated with serious post-surgical complications. Therefore, new strategies to help re-establish heart functionality are necessary. Tissue engineering and stem cell therapy are the promising approaches that are being explored for the treatment of MI. The stem cell niche is extremely important for the proliferation and differentiation of stem cells and tissue regeneration. For the introduction of stem cells into the host tissue an artificial carrier such as a scaffold is preferred as direct injection of stem cells has resulted in fast stem cell death. Such scaffold will provide the proper microenvironment that can be altered electronically to provide temporal stimulation to the cells. We have developed an electroactive polymer (EAP) scaffold for cardiac tissue engineering. The EAP scaffold mimics the extracellular matrix and provides a 3D microenvironment that can be easily tuned during fabrication, such as controllable fibre dimensions, alignment, and coating. In addition, the scaffold can provide electrical and electromechanical stimulation to the stem cells which are important external stimuli to stem cell differentiation. We tested the initial biocompatibility of these scaffolds using cardiac progenitor cells (CPCs), and continued onto more sensitive induced pluripotent stem cells (iPS). We present the fabrication and characterisation of these electroactive fibres as well as the response of increasingly sensitive cell types to the scaffolds.

Place, publisher, year, edition, pages
SPIE - International Society for Optical Engineering, 2015
Series
Proceedings of SPIE, ISSN 0277-786X ; 9430
National Category
Medical Materials
Identifiers
urn:nbn:se:liu:diva-118260 (URN)10.1117/12.2084165 (DOI)000355580900052 ()
Conference
Electroactive Polymer Actuators and Devices (EAPAD) 2015
Available from: 2015-05-22 Created: 2015-05-22 Last updated: 2018-10-11Bibliographically approved
Henningsson, A. J., Wilhelmsson, P., Gyllemark, P., Kozak Ljunggren, M., Matussek, A., Nyman, D., . . . Forsberg, P. (2015). Low risk of seroconversion or clinical disease in humans after a bite by an Anaplasma phagocytophilum-infected tick. Ticks and Tick-borne Diseases, 6(6), 787-792
Open this publication in new window or tab >>Low risk of seroconversion or clinical disease in humans after a bite by an Anaplasma phagocytophilum-infected tick
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2015 (English)In: Ticks and Tick-borne Diseases, ISSN 1877-959X, E-ISSN 1877-9603, Vol. 6, no 6, p. 787-792Article in journal (Refereed) Published
Abstract [en]

The risk of contracting human granulocytic anaplasmosis (HGA) after a tick bite is mainly unknown. In this study we investigated the clinical and serological response in 30 humans bitten by ticks positive for Anaplasma phagocytophilum (Group A), 30 humans bitten by Borrelia burgdorferi sensu lato (s.l.)-positive ticks (Group B), and 30 humans bitten by ticks negative for both A. phagocytophilum and B. burgdorferi s.l. (Group C). Ticks, blood samples and questionnaires were collected from tick-bitten humans at 34 primary healthcare centres in Sweden and in the Åland Islands, Finland, at the time of the tick bite and after three months. A total of 2553 ticks detached from humans in 2007-2009 were analyzed by polymerase chain reaction, and 31 (1.2%) were positive for A. phagocytophilum, 556 (21.8%) were positive for B. burgdorferi s.l., and eight (0.3%) were co-infected by A. phagocytophilum and B. burgdorferi s.l. The overall prevalence of Anaplasma IgG antibodies in the included participants (n=90) was 17%, and there was no significant difference between the groups A-C. Only one of the participants (in Group C) showed a four-fold increase of IgG antibodies against A. phagocytophilum at the three-month follow-up, but reported no symptoms. The frequency of reported symptoms did not differ between groups A-C, and was unrelated to the findings of A. phagocytophilum and B. burgdorferi s.l. in the detached ticks. We conclude that the risk for HGA or asymptomatic seroconversion after a tick bite in Sweden or in the Åland Islands is low, even if the tick is infected by A. phagocytophilum.

Place, publisher, year, edition, pages
Elsevier, 2015
National Category
Microbiology in the medical area
Identifiers
urn:nbn:se:liu:diva-122245 (URN)10.1016/j.ttbdis.2015.07.005 (DOI)000362143800015 ()26187418 (PubMedID)
Note

Funding text: Swedish Research Council; Medical Research Council of Southeast Sweden (FORSS); Futurum Academy of Healthcare; Jonkoping County Council; Interreg IV A Programme ScandTick; Division of Medical Services, Ryhov County Hospital, Jonkoping

Available from: 2015-10-26 Created: 2015-10-26 Last updated: 2018-01-10
Kozak Ljunggren, M. & Griffith, M. (2014). Animal models ad in vitro alternatives in regenerative medicine: Focus on biomaterials development. In: Mayuri S. Prasad & Paolo Di Nardo (Ed.), Innovative Strategies in Tissue Engineering: (pp. 37-51). Aalborg, Denmark: River Publishers
Open this publication in new window or tab >>Animal models ad in vitro alternatives in regenerative medicine: Focus on biomaterials development
2014 (English)In: Innovative Strategies in Tissue Engineering / [ed] Mayuri S. Prasad & Paolo Di Nardo, Aalborg, Denmark: River Publishers, 2014, p. 37-51Chapter in book (Other academic)
Place, publisher, year, edition, pages
Aalborg, Denmark: River Publishers, 2014
Series
The River Publishers Series in Research and Business Chronicles: Biotechnology and Medicine
National Category
Basic Medicine
Identifiers
urn:nbn:se:liu:diva-111489 (URN)9788793237094 (ISBN)
Available from: 2014-10-19 Created: 2014-10-19 Last updated: 2018-01-11
Gelmi, A., Kozak Ljunggren, M., Rafat, M. & Jager, E. (2014). Bioelectronic nanofibre scaffolds for tissue engineering and whole-cell biosensors. In: : . Paper presented at 24th Anniversary World Congress on Biosensors – Biosensors 2014. 24th Anniversary World Congress on Biosensors – Biosensors 2014, 27-30 May 2014, Melbourne, Australia..
Open this publication in new window or tab >>Bioelectronic nanofibre scaffolds for tissue engineering and whole-cell biosensors
2014 (English)Conference paper, Oral presentation only (Refereed)
National Category
Medical Engineering
Identifiers
urn:nbn:se:liu:diva-128245 (URN)
Conference
24th Anniversary World Congress on Biosensors – Biosensors 2014. 24th Anniversary World Congress on Biosensors – Biosensors 2014, 27-30 May 2014, Melbourne, Australia.
Available from: 2016-05-23 Created: 2016-05-23 Last updated: 2018-10-11
Kozak Ljunggren, M., Elizondo, R. A., Edin, J., Olsen, D., Merrett, K., Lee, C.-J., . . . Griffith, M. (2014). Effect of Surgical Technique on Corneal Implant Performance. Translational Vision Science & Technology, 3(2), 1-13
Open this publication in new window or tab >>Effect of Surgical Technique on Corneal Implant Performance
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2014 (English)In: Translational Vision Science & Technology, ISSN 2164-2591, Vol. 3, no 2, p. 1-13Article in journal (Refereed) Published
Abstract [en]

Purpose: Our aim was to determine the effect of a surgical technique on biomaterial implant performance, specifically graft retention.

 

Methods: Twelve mini pigs were implanted with cell-free, 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS) cross-linked recombinant human collagen type III (RHCIII) hydrogels as substitutes for donor corneal allografts using overlying sutures with or without human amniotic membrane (HAM) versus interrupted sutures with HAM. The effects of the retention method were compared as well as the effects of collagen concentration (13.7% to 15% RHCIII).

Results: All implanted corneas showed initial haze that cleared with time, resulting in corneas with optical clarity matching those of untreated controls. Biochemical analysis showed that by 12 months post operation, the initial RHCIII implants had been completely remodeled, as type I collagen, was the major collagenous protein detected, whereas no RHCIII could be detected. Histological analysis showed all implanted corneas exhibited regeneration of epithelial and stromal layers as well as nerves, along with touch sensitivity and tear production. Most neovascularization was seen in corneas stabilized by interrupted sutures.

Conclusions: This showed that the surgical technique used does have a significant effect on the overall performance of corneal implants, overlying sutures caused less vascularization than interrupted sutures.

Translational Relevance: Understanding the significance of the suturing technique can aid the selection of the most appropriate procedure when implanting artificial corneal substitutes. The same degree of regeneration, despite a higher collagen content indicates that future material development can progress toward stronger, more resistant implants.

Place, publisher, year, edition, pages
Association for Research in Vision and Ophthalmology, 2014
Keywords
biosynthetic cornea; corneal regeneration; biomaterials; recombinant human collagen; corneal transplantation
National Category
Medical and Health Sciences Biomaterials Science Ophthalmology
Identifiers
urn:nbn:se:liu:diva-108585 (URN)10.1167/tvst.3.2.6 (DOI)24749003 (PubMedID)
Available from: 2014-06-30 Created: 2014-06-30 Last updated: 2015-08-10Bibliographically approved
Gelmi, A., Kozak Ljunggren, M., Rafat, M. & Jager, E. (2014). Influence of conductive polymer doping on the viability of cardiac progenitor cells. Journal of materials chemistry. B, 2(24), 3860-3867
Open this publication in new window or tab >>Influence of conductive polymer doping on the viability of cardiac progenitor cells
2014 (English)In: Journal of materials chemistry. B, ISSN 2050-750X, E-ISSN 2050-7518, Vol. 2, no 24, p. 3860-3867Article in journal (Refereed) Published
Abstract [en]

Cardiac tissue engineering via the use of stem cells is the future for repairing impaired heart function that results from a myocardial infarction. Developing an optimised platform to support the stem cells is vital to realising this, and through utilising new smart materials such as conductive polymers we can provide a multi-pronged approach to supporting and stimulating the stem cells via engineered surface properties, electrical, and electromechanical stimulation. Here we present a fundamental study on the viability of cardiac progenitor cells on conductive polymer surfaces, focusing on the impact of surface properties such as roughness, surface energy, and surface chemistry with variation of the polymer dopant molecules. The conductive polymer materials were shown to provide a viable support for both endothelial and cardiac progenitor cells, while the surface energy and roughness were observed to influence viability for both progenitor cell types. Characterising the interaction between the cardiac progenitor cells and the conductive polymer surface is a critical step towards optimising these materials for cardiac tissue regeneration, and this study will advance the limited knowledge on biomaterial surface interactions with cardiac cells.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2014
National Category
Engineering and Technology
Identifiers
urn:nbn:se:liu:diva-108817 (URN)10.1039/c4tb00142g (DOI)000336853400015 ()
Available from: 2014-07-07 Created: 2014-07-06 Last updated: 2017-12-05Bibliographically approved
Gelmi, A., Kozak Ljunggren, M., Rafat, M. & Jager, E. (2014). Smart Electroactive Scaffolds for Cardiac Tissue Regeneration. In: : . Paper presented at NanoMed, International Conference on Nanotechnology in Medicine.
Open this publication in new window or tab >>Smart Electroactive Scaffolds for Cardiac Tissue Regeneration
2014 (English)Conference paper, Oral presentation only (Refereed)
National Category
Medical Engineering
Identifiers
urn:nbn:se:liu:diva-128243 (URN)
Conference
NanoMed, International Conference on Nanotechnology in Medicine
Available from: 2016-05-23 Created: 2016-05-23 Last updated: 2018-10-11
Gelmi, A., Ljunggren, M., Rafat, M. & Jager, E. (2013). Electroactive scaffolds for cardiac tissue regeneration. In: : . Paper presented at EuroEAP 2013. Third international conference on Electromechanically Active Polymer (EAP) transducers and artificial muscles, June 25-26, Dübendorf (Zürich), Switzerland.
Open this publication in new window or tab >>Electroactive scaffolds for cardiac tissue regeneration
2013 (English)Conference paper, Poster (with or without abstract) (Other academic)
Abstract [en]

Myocardial Infarction (MI), commonly known as a heart attack, is the interruption of blood supply to a part of the heart, causing heart cells to die. In order to restore function by-pass surgery or ultimately heart transplantation is needed. However, due to the shortage of organ donors and complications associated with immune suppressive treatments, development of new strategies to help regenerate the injured heart is necessary. Stem cell therapy can be used to repair necrotic heart tissue and achieve myocardial regeneration. This research is focused on developing implantable electroactive fiber scaffolds that will increase the differentiation ratio of mesenchymal stem cells into cardiomyocytes and thus increase the formation of novel cardiac tissue to repair or replace the damaged cardiac tissue after MI. Composite nanofibrous scaffold of poly(dl-lactide-co-glycolide) (PLGA) have been coated with biodoped polypyrrole to create an electroactive fiber scaffold, with controllable fiber dimensions and alignment. The electrical properties of the polymers are an integral factor in creating these 'intelligent' 3-D materials; not only does the inherent conductivity provide a platform for electrical stimulation, but the ionic actuation of the polymer can also provide mechanical stimulation to the seeded cells. The biocompatibility of the polymer, PLGA scaffolds, and coated PLGA scaffolds has been investigated using primary cardiovascular progenitor cells.

National Category
Biomaterials Science
Identifiers
urn:nbn:se:liu:diva-100530 (URN)
Conference
EuroEAP 2013. Third international conference on Electromechanically Active Polymer (EAP) transducers and artificial muscles, June 25-26, Dübendorf (Zürich), Switzerland
Available from: 2013-11-08 Created: 2013-11-08 Last updated: 2014-10-08Bibliographically approved
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