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Atikuzzaman, MohammadORCID iD iconorcid.org/0000-0001-8425-7505
Publications (5 of 5) Show all publications
Alvarez-Rodriguez, M., Atikuzzaman, M., Venhoranta, H., Wright, D. & Rodriguez-Martinez, H. (2019). Expression of Immune Regulatory Genes in the Porcine Internal Genital Tract Is Differentially Triggered by Spermatozoa and Seminal Plasma. International Journal of Molecular Sciences, 20(3), Article ID 513.
Open this publication in new window or tab >>Expression of Immune Regulatory Genes in the Porcine Internal Genital Tract Is Differentially Triggered by Spermatozoa and Seminal Plasma
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2019 (English)In: International Journal of Molecular Sciences, ISSN 1422-0067, E-ISSN 1422-0067, Vol. 20, no 3, article id 513Article in journal (Refereed) Published
Abstract [en]

Mating or cervical deposition of spermatozoa or seminal plasma (SP) modifies the expression of genes affecting local immune defense processes at the oviductal sperm reservoir in animals with internal fertilization, frequently by down-regulation. Such responses may occur alongside sperm transport to or even beyond the reservoir. Here, immune-related gene expression was explored with cDNA microarrays on porcine cervix-to-infundibulum tissues, pre-/peri-ovulation. Samples were collected 24 h post-mating or cervical deposition of sperm-peak spermatozoa or SP (from the sperm-peak fraction or the whole ejaculate). All treatments of this interventional study affected gene expression. The concerted action of spermatozoa and SP down-regulated chemokine and cytokine (P00031), interferon-gamma signaling (P00035), and JAK/STAT (P00038) pathways in segments up to the sperm reservoir (utero-tubal junction (UTJ)/isthmus). Spermatozoa in the vanguard sperm-peak fraction (P1-AI), uniquely displayed an up-regulatory effect on these pathways in the ampulla and infundibulum. Sperm-free SP, on the other hand, did not lead to major effects on gene expression, despite the clinical notion that SP mitigates reactivity by the female immune system after mating or artificial insemination.

Place, publisher, year, edition, pages
MDPI, 2019
Keywords
transcriptomics; microarray; bioinformatics; spermatozoa; seminal plasma; immune-regulation; female internal genitalia; pig
National Category
Medical Genetics
Identifiers
urn:nbn:se:liu:diva-156214 (URN)10.3390/ijms20030513 (DOI)000462412500057 ()30691059 (PubMedID)2-s2.0-85060587196 (Scopus ID)
Note

Funding Agencies|Research Council FORMAS, Stockholm [2017-00946]; Swedish Research Council (Vetenskapsradet, VR) [2015-05919]

Available from: 2019-04-09 Created: 2019-04-09 Last updated: 2019-10-18Bibliographically approved
Rodriguez-Martinez, H., Tienthai, P., Atikuzzaman, M., Vicente Carrillo, A., Rubér, M. & Alvarez-Rodriguez, M. (2016). The ubiquitous hyaluronan: Functionally implicated in the oviduct?. Theriogenology, 86(1), 182-186
Open this publication in new window or tab >>The ubiquitous hyaluronan: Functionally implicated in the oviduct?
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2016 (English)In: Theriogenology, ISSN 0093-691X, E-ISSN 1879-3231, Vol. 86, no 1, p. 182-186Article, review/survey (Refereed) Published
Abstract [en]

Hyaluronan (hyaluronic acid) is a simple, nonantigenic, nonsulfated glycosaminoglycan present everywhere in the extracellular compartments of the body. Noteworthy, it is highly conserved phylogenetically, from sauropsida to mammals; and plays a plethora of roles from embryonic/fetal development to adult physiological and pathological events, including tumor development. In reproduction, hyaluronan has proven related to initial events as sperm survival, buildup of the sperm reservoir in the oviduct, regulation of sperm capacitation, and prefertilization to later participate in embryo, fetal, and placental development. Synthesis, binding (via the CD44 membrane receptor), and degradation of hyaluronan occur in male and female genital organs, the oviduct being no exception. This review discusses our current knowledge on roles of this ubiquitous glycosaminoglycan on the survival of immunologically foreign spermatozoa in the pig oviduct, a relevant event for fertility. During preovulatory storage in the functional tubal sperm reservoir, spermatozoa are entrapped in a mucus-like tubal fluid. This fluid contains fluctuating levels of hyaluronan, which is synthesized by the lining epithelium by hyaluronan synthase 3. Both hyaluronan and its CD44 receptor are particularly evident in the deep mucosal furrows of the sperm reservoir, in which most spermatozoa are embedded in; kept alive, uncapacitated but also undetected by the immune system of the female. Hyaluronan is also present in the seminal plasma, and evidence points toward an involvement of hyaluronan and its receptor in the local (tubal and possibly uterine) production of antiinflammatory cytokines, such as interleulcin-10, pertaining maternal immune tolerance of these foreign cells. (C) 2015 Elsevier Inc. All rights reserved.

Place, publisher, year, edition, pages
ELSEVIER SCIENCE INC, 2016
Keywords
Hyaluronan; Sperm oviduct reservoir; Sperm survival; Capacitation; Immunity; Pig
National Category
Obstetrics, Gynecology and Reproductive Medicine
Identifiers
urn:nbn:se:liu:diva-130123 (URN)10.1016/j.theriogenology.2015.11.025 (DOI)000377643700020 ()26768539 (PubMedID)
Note

Funding Agencies|Swedish Research Council VR (Vetenskapsradet) [521-2011-6353]; Swedish Research Council Formas, Stockholm, Sweden [221-2011-512]

Available from: 2016-07-12 Created: 2016-07-11 Last updated: 2017-11-28
Atikuzzaman, M., Bhai Mehta, R., Fogelholm, J., Wright, D. & Rodriguez-Martinez, H. (2015). Mating induces the expression of immune- and pH-regulatory genes in the utero-vaginal junction containing mucosal sperm-storage tubuli of hens. Reproduction, 150(6), 473-483
Open this publication in new window or tab >>Mating induces the expression of immune- and pH-regulatory genes in the utero-vaginal junction containing mucosal sperm-storage tubuli of hens
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2015 (English)In: Reproduction, Vol. 150, no 6, p. 473-483Article in journal (Refereed) Published
Abstract [en]

The female chicken, as with other species with internal fertilization, can tolerate the presence of spermatozoa within specialized sperm-storage tubuli (SST) located in the mucosa of the utero-vaginal junction (UVJ) for days or weeks, without eliciting an immune response. To determine if the oviduct alters its gene expression in response to sperm entry, segments from the oviduct (UVJ, uterus, isthmus, magnum and infundibulum) of mated and unmated (control) hens, derived from an advanced inter-cross line between Red Junglefowl and White Leghorn, were explored 24 h after mating using cDNA microarray analysis. Mating shifted the expression of fifteen genes in the UVJ (53.33% immune-modulatory and 20.00% pH-regulatory) and seven genes in the uterus, none of the genes in the latter segment overlapping the former (with the differentially expressed genes themselves being less related to immune-modulatory function). The other oviductal segments did not show any significant changes. These findings suggest sperm deposition causes a shift in expression in the UVJ (containing mucosal SST) and the uterus for genes involved in immune-modulatory and pH-regulatory functions, both relevant for sperm survival in the hen's oviduct.

Place, publisher, year, edition, pages
Bioscientifica, 2015
National Category
Genetics
Identifiers
urn:nbn:se:liu:diva-122573 (URN)10.1530/REP-15-0253 (DOI)000365344400004 ()26370241 (PubMedID)
Note

Funding agencies: Research Council FORMAS, Stockholm [221-2011-512]; FORMAS [221-2012-667]; VR [621-2011-4802]

Available from: 2015-11-09 Created: 2015-11-09 Last updated: 2017-02-20
Barranco, I., Roca, J., Tvarijonaviciute, A., Rubér, M., Vicente Carrillo, A., Atikuzzaman, M., . . . Rodriguez-Martinez, H. (2015). Measurement of Activity and Concentration of Paraoxonase 1 (PON-1) in Seminal Plasma and Identification of PON-2 in the Sperm of Boar Ejaculates. Molecular Reproduction and Development, 82(1), 58-65
Open this publication in new window or tab >>Measurement of Activity and Concentration of Paraoxonase 1 (PON-1) in Seminal Plasma and Identification of PON-2 in the Sperm of Boar Ejaculates
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2015 (English)In: Molecular Reproduction and Development, ISSN 1040-452X, E-ISSN 1098-2795, Vol. 82, no 1, p. 58-65Article in journal (Refereed) Published
Abstract [en]

This study revealed and characterised the presence of the antioxidant enzymes paraoxonase (PON) type 1 (PON-1, extracellular) and type 2 (PON-2, intracellular) in boar semen. To evaluate PON-1, an entire ejaculate from each of ten boars was collected and the seminal plasma was harvested after double centrifugation (1,500g for 10min). Seminal plasma was analysed for concentration as well as enzymatic activity of PON-1 and total cholesterol levels. Seminal-plasma PON-1 concentration ranged from 0.961 to 1.670ng/ml while its enzymatic activity ranged from 0.056 to 0.400 IU/ml, which represent individual variance. Seminal-plasma PON-1 concentration and enzymatic activity were negatively correlated (r=-0.763; Pless than0.01). The activity of seminal-plasma PON-1 negatively correlated with ejaculate volume (r=-0.726, Pless than0.05), but positively correlated with sperm concentration (r=0.654, Pless than0.05). Total seminal-plasma cholesterol concentration positively correlated with PON-1 activity (r=0.773; Pless than0.01), but negatively correlated with PON-1 concentration (r=-0.709; Pless than0.05). The presence of intracellular PON-2 was determined via immunocytochemistry in spermatozoa derived from artificial insemination. PON-2 localised to the post-acrosomal area of the sperm head and principal piece of the tail in membrane-intact spermatozoa. In summary, PON is present in boar semen, with PON-1 at low levels in seminal plasma and PON-2 within the spermatozoa. Further studies are needed to characterise the relationship between antioxidant PONs with sperm and other seminal-plasma parameters. Mol. Reprod. Dev. 82: 58-65, 2015. (c) 2014 Wiley Periodicals, Inc.

Place, publisher, year, edition, pages
Wiley-Blackwell, 2015
National Category
Medical Bioscience
Identifiers
urn:nbn:se:liu:diva-114595 (URN)10.1002/mrd.22444 (DOI)000348656700007 ()25487823 (PubMedID)
Note

Funding Agencies|Ministry of Economy and Competitiveness of Spain; European Regional Development Fund [AGL2012-39903]; Swedish Research Council Formas [2011-512]; Ministry of Education, Culture and Sport of Spain [FPU12/05008]

Available from: 2015-02-27 Created: 2015-02-26 Last updated: 2017-12-04
Barranco, I., Rubér, M., Perez-Patino, C., Atikuzzaman, M., Martinez, E. A., Roca, J. & Rodriguez-Martinez, H. (2015). The Seminal Plasma of the Boar is Rich in Cytokines, with Significant Individual and Intra-Ejaculate Variation. American Journal of Reproductive Immunology, 74(6), 523-532
Open this publication in new window or tab >>The Seminal Plasma of the Boar is Rich in Cytokines, with Significant Individual and Intra-Ejaculate Variation
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2015 (English)In: American Journal of Reproductive Immunology, ISSN 1046-7408, E-ISSN 1600-0897, Vol. 74, no 6, p. 523-532Article in journal (Refereed) Published
Abstract [en]

Problem The boar, as human, sequentially ejaculates sperm-rich and sperm-poor fractions. Seminal plasma (SP) spermadhesins (PSP-I/PSP-II) induce a primary endometrial inflammatory response in female sows, similar to that elicited by semen deposition in other species, including human. However, the SP is also known to mitigate such response, making it transient to allow for embryo entry to a cleansed endometrium. Although cytokine involvement has been claimed, the exploration of cytokines in different SP fractions is scarce. This study determines Th1, Th2, Th17 and Th3 cytokine profiles in specific ejaculate SP fractions from boars of proven fertility. Methods SP samples from the sperm-rich fraction (SRF) and the sperm-poor post-SRF fraction (post-SRF) of manually collected ejaculates from eight boars (four ejaculates per boar) were analysed by commercial multiplex bead assay kits (Milliplex MAP, Millipore, USA) for interferon-gamma, interferon gamma-induced protein 10, macrophage-derived chemokine, growth-regulated oncogene, granulocyte-macrophage colony-stimulating factor, monocyte chemo-attractant protein-1, interleukins (IL)-6, IL-8, IL-10, IL-15, IL-17 and transforming growth factor (TGF)-beta 1-beta 3. Results Cytokine concentrations differed between the ejaculate fractions among boars, being highest in the post-SRF. Conclusion Boar SP is rich in Th1, Th2, Th17 and Th3 cytokines, with lowest concentrations in the sperm-peak-containing fraction, indicating its main immune influence might reside in the larger, protein-rich sperm-poor post-SRF.

Place, publisher, year, edition, pages
WILEY-BLACKWELL, 2015
Keywords
Ejaculate fractions; immunomodulatory molecules; pig; seminal plasma peptides
National Category
Clinical Medicine
Identifiers
urn:nbn:se:liu:diva-124497 (URN)10.1111/aji.12432 (DOI)000367669300006 ()26412440 (PubMedID)
Note

Funding Agencies|MINECO Madrid (Spain) [AGL2012-39903]; FEDER funds (EU); Formas (Stockholm, Sweden); MECD (Madrid, Spain); Seneca Foundation (Murcia, Spain)

Available from: 2016-02-02 Created: 2016-02-01 Last updated: 2017-11-30
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ORCID iD: ORCID iD iconorcid.org/0000-0001-8425-7505

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