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Carlsson, Anders K
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Publications (4 of 4) Show all publications
Wåhlén, K., Olausson, P., Carlsson, A. K., Ghafouri, N., Gerdle, B. & Ghafouri, B. (2017). Systemic alterations in plasma proteins from women with chronic widespread pain compared to healthy controls: a proteomic study. Journal of Pain Research, 10, 797-809
Open this publication in new window or tab >>Systemic alterations in plasma proteins from women with chronic widespread pain compared to healthy controls: a proteomic study
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2017 (English)In: Journal of Pain Research, ISSN 1178-7090, E-ISSN 1178-7090, Vol. 10, p. 797-809Article in journal (Refereed) Published
Abstract [en]

Chronic widespread pain (CWP) is a complex pain condition that is difficult to treat. The prevalence of CWP approximates similar to 10% of the general population, with higher prevalence in women. Lack of understanding of molecular mechanisms has been a challenge for diagnosis and treatment of chronic pain. The aim of this study was to explore the systemic protein changes in CWP compared to those in healthy controls (CON). By applying 2-dimensional gel electrophoresis, we analyzed the protein pattern of plasma samples from women with CWP (n=16) and healthy women (n=23). The proteomic data were analyzed using multivariate statistical models, and altered proteins were identified using mass spectrometry. The proteome analysis was further validated by gel-free Western blot. Multivariate statistical data analysis of quantified proteins revealed 22 altered proteins in women with CWP, compared to CON group. Many of the identified proteins are previously known to be involved in different parts of the complement system and metabolic and inflammatory processes, e.g., complement factor B, vitamin D-binding protein, ceruloplasmin, transthyretin and alpha-2-HS-glycoprotein. These results indicate that important systemic protein differences exist between women with CWP and healthy women. Further, this study illustrates the potential use of proteomics to detect biomarkers that may provide new insights into the molecular mechanism(s) of chronic pain. However, further larger investigations are required in order to confirm these findings before it will be possible to identify proteins as potential pain biomarkers for clinical use.

Place, publisher, year, edition, pages
DOVE MEDICAL PRESS LTD, 2017
Keywords
inflammation; biomarker; painomics; complement system; GC protein
National Category
Rheumatology and Autoimmunity
Identifiers
urn:nbn:se:liu:diva-136895 (URN)10.2147/JPR.S128597 (DOI)000398611900001 ()28435317 (PubMedID)
Note

Funding Agencies|Swedish Council for Working Life and Social Research [2010-0913]; Swedish Research Council [K2015-99X-21874-05-04]; Medical Research Council of Southeast Sweden [159031]; AFA Insurance [Dnr-140341]; Region Ostergotland research fund [LIO445161, SC-2013-00395-36]; Ake Wiberg foundation

Available from: 2017-04-29 Created: 2017-04-29 Last updated: 2018-04-18
Ghafouri, B., Carlsson, A., Holmberg, S., Thelin, A. & Tagesson, C. (2016). Biomarkers of lsystemic inflammation in farmers with musculoskeletal disorders; a plasma proteomic study. BMC Musculoskeletal Disorders, 17(206)
Open this publication in new window or tab >>Biomarkers of lsystemic inflammation in farmers with musculoskeletal disorders; a plasma proteomic study
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2016 (English)In: BMC Musculoskeletal Disorders, ISSN 1471-2474, E-ISSN 1471-2474, Vol. 17, no 206Article in journal (Refereed) Published
Abstract [en]

Background: Farmers have an increased risk for musculoskeletal disorders (MSD) such as osteoarthritis of the hip, low back pain, and neck and upper limb complaints. The underlying mechanisms are not fully understood. Workrelated exposures and inflammatory responses might be involved. Our objective was to identify plasma proteins that differentiated farmers with MSD from rural referents. Methods: Plasma samples from 13 farmers with MSD and rural referents were included in the investigation. Gel based proteomics was used for protein analysis and proteins that differed significantly between the groups were identified by mass spectrometry. Results: In total, 15 proteins differed significantly between the groups. The levels of leucine-rich alpha-2-glycoprotein, haptoglobin, complement factor B, serotransferrin, one isoform of kininogen, one isoform of alpha-1-antitrypsin, and two isoforms of hemopexin were higher in farmers with MSD than in referents. On the other hand, the levels of alpha-2-HS-glycoprotein, alpha-1B-glycoprotein, vitamin D-binding protein, apolipoprotein A1, antithrombin, one isoform of kininogen, and one isoform of alpha-1-antitrypsin were lower in farmers than in referents. Many of the identified proteins are known to be involved in inflammation. Conclusions: Farmers with MSD had altered plasma levels of protein biomarkers compared to the referents, indicating that farmers with MSD may be subject to a more systemic inflammation. It is possible that the identified differences of proteins may give clues to the biochemical changes occurring during the development and progression of MSD in farmers, and that one or several of these protein biomarkers might eventually be used to identify and prevent work-related MSD.

Place, publisher, year, edition, pages
BIOMED CENTRAL LTD, 2016
Keywords
Musculoskeletal disorder; Farmers; Proteomic; Systemic inflammation; Occupational medicine
National Category
Basic Medicine
Identifiers
urn:nbn:se:liu:diva-128936 (URN)10.1186/s12891-016-1059-y (DOI)000375761500001 ()27160764 (PubMedID)
Note

Funding Agencies|Swedish Farmers Foundation for Agricultural Research [H0935072]; Swedish Rheumatism Association [R-420491]

Available from: 2016-06-09 Created: 2016-06-07 Last updated: 2018-01-10
Hadrévi, J., Turkina, M., Carlsson, A., Gerdle, B., Larsson, B., Hellström, F. & Ghafouri, B. (2016). Myosin light chain and calcium regulating protein differences in chronic musculoskeletal neck and shoulder pain. Journal of Integrated OMICS, 6(1), 1-8
Open this publication in new window or tab >>Myosin light chain and calcium regulating protein differences in chronic musculoskeletal neck and shoulder pain
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2016 (English)In: Journal of Integrated OMICS, ISSN 2182-0287, Vol. 6, no 1, p. 1-8Article in journal (Refereed) Published
Abstract [en]

Proteomic screening analysis has detected myosin light chain (MLC) as a protein implied to be involved in chronic musculoskeletal neck and shoulder pain. Several analyses of MLC proteins have stated a difference in phosphorylation being the determining factor for protein activation hence altered contrability of the muscle in i.e. senescence. In continuation of a previous publication, this study is an attempt to analyze the different MLC isoforms by mass spectrometry and immune-analyses in myalgic and healthy trapezius muscle. In the present study no differences in phosphorylation level between the corresponding individual proteins were detected using LC-MSMS and immunoblotting; instead we assigned different isoforms of regulatory MLCs. To further elucidate the contrability: calcium (Ca2+) regulatory proteins, sarco(endo)plasmic reticulum Ca2+ ATPase 1 (SERCA-1) and calsequestrine (CSQ) were analyzed by western blot. The analysis revealed a significantly increased abundance of SERCA-1 protein in the myalgic muscle and a significantly increased abundance of CSQ in healthy muscle. Myalgic muscle contraction patterns have in previous studies shown to differ from healthy muscle which may be connected to the Ca2+ availability in the muscle. Here we present the proteomic characterization of differences in Ca2+ regulating proteins and particularly regulatory MLCs in trapezius muscle of women with chronic musculoskeletal neck and shoulder pain.

Place, publisher, year, edition, pages
Proteomass Scientific Society, 2016
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:liu:diva-130539 (URN)10.5584/jiomics.v6i1.191 (DOI)
Available from: 2016-08-15 Created: 2016-08-15 Last updated: 2018-01-17
Bäckryd, E., Ghafouri, B., Carlsson, A. K., Olausson, P. & Gerdle, B. (2015). Multivariate proteomic analysis of the cerebrospinal fluid of patients with peripheral neuropathic pain and healthy controls: a hypothesis-generating pilot study. Journal of Pain Research, 8, 321-333
Open this publication in new window or tab >>Multivariate proteomic analysis of the cerebrospinal fluid of patients with peripheral neuropathic pain and healthy controls: a hypothesis-generating pilot study
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2015 (English)In: Journal of Pain Research, ISSN 1178-7090, E-ISSN 1178-7090, Vol. 8, p. 321-333Article in journal (Refereed) Published
Abstract [en]

Pain medicine lacks objective biomarkers to guide diagnosis and treatment. Combining two-dimensional gel proteomics with multivariate data analysis by projection, we exploratively analyzed the cerebrospinal fluid of eleven patients with severe peripheral neuropathic pain due to trauma and/or surgery refractory to conventional treatment and eleven healthy controls. Using orthogonal partial least squares discriminant analysis, we identified a panel of 36 proteins highly discriminating between the two groups. Due to a possible confounding effect of age, a new model with age as outcome variable was computed for patients (n=11), and four out of 36 protein spots were excluded due to a probable influence of age. Of the 32 remaining proteins, the following seven had the highest discriminatory power between the two groups: an isoform of angiotensinogen (upregulated in patients), two isoforms of alpha-1-antitrypsin (downregulated in patients), three isoforms of haptoglobin (upregulated in patients), and one isoform of pigment epithelium-derived factor (downregulated in patients). It has recently been hypothesized that the renin–angiotensin system may play a role in the pathophysiology of neuropathic pain, and a clinical trial of an angiotensin II receptor antagonist was recently published. It is noteworthy that when searching for neuropathic pain biomarkers with a purely explorative methodology, it was indeed a renin–angiotensin system protein that had the highest discriminatory power between patients and controls in the present study. The results from this hypothesis-generating pilot study have to be confirmed in larger, hypothesis-driven studies with age-matched controls, but the present study illustrates the fruitfulness of combining proteomics with multivariate data analysis in hypothesis-generating pain biomarker studies in humans.

Place, publisher, year, edition, pages
Dovepress, 2015
Keywords
Cerebrospinal fluid, multivariate data analysis, neuropathic pain, proteomics
National Category
Nursing Surgery
Identifiers
urn:nbn:se:liu:diva-121493 (URN)10.2147/JPR.S82970 (DOI)000364718500002 ()26170714 (PubMedID)
Note

Funding agencies: Swedish Research Council; Swedish Council for Working Life and Social Research [2010-0913]; County Council of Ostergotland (Sinnescentrum); Halsofonden foundation

Available from: 2015-09-22 Created: 2015-09-22 Last updated: 2017-12-05Bibliographically approved
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