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Alexandre, Campos
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Publications (2 of 2) Show all publications
Campos, A., Danielsson, G., Farinha, A. P., Kuruvilla, J., Warholm, P. & Cristobal, S. (2016). Shotgun proteomics to unravel marine mussel (Mytilus edulis) response to long-term exposure to low salinity and propranolol in a Baltic Sea microcosm. Journal of Proteomics, 137, 97-106
Open this publication in new window or tab >>Shotgun proteomics to unravel marine mussel (Mytilus edulis) response to long-term exposure to low salinity and propranolol in a Baltic Sea microcosm
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2016 (English)In: Journal of Proteomics, ISSN 1874-3919, E-ISSN 1876-7737, Vol. 137, p. 97-106Article in journal (Refereed) Published
Abstract [en]

Pharmaceuticals, among them the β-adrenoreceptor blocker propranolol, are an important group of environmental contaminants reported in European waters. Laboratory exposure to pharmaceuticals on marine species has been performed without considering the input of the ecosystem flow. To unravel the ecosystem response to long-term exposure to propranolol we have performed long-term exposure to propranolol and low salinity in microcosms. We applied shotgun proteomic analysis to gills of Mytilus edulis from those Baltic Sea microcosms and identified 2071 proteins with a proteogenomic strategy. The proteome profiling patterns from the 587 highly reproductive proteins among groups define salinity as a key factor in the mussel´s response to propranolol. Exposure at low salinity drives molecular mechanisms of adaptation based on a decrease in the abundance of several cytoskeletal proteins, signalling and intracellular membrane trafficking pathway combined with a response towards the maintenance of transcription and translation. The exposure to propranolol combined with low salinity modulates the expression of structural proteins including cilia functions and decrease the expression membrane protein transporters. This study reinforces the environment concerns of the impact of low salinity in combination with anthropogenic pollutants and anticipate critical physiological conditions for the survival of the blue mussel in the northern areas.

Place, publisher, year, edition, pages
Elsevier, 2016
Keywords
Mytilus edulis, shotgun proteomics, propranolol, low salinity, environmental monitoring, climate change
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:liu:diva-124213 (URN)10.1016/j.jprot.2016.01.010 (DOI)000374368800010 ()
Funder
Swedish Research Council
Note

Funding agencies: Swedish Research Council-Natural Science; VR-NT; Carl Trygger Foundation; Oscar and Lilli Lamms Minne Foundation; Angpanneforening Research Foundation; Magnus Bergsvall Foundation; IKERBASQUE; Basque Foundation for Science; VINNOVA; County Council of Oste

Available from: 2016-01-22 Created: 2016-01-22 Last updated: 2017-11-30Bibliographically approved
Alexandre, C., Apraiz, I., da Fonseca, R. R. & Cristobal, S. (2015). Shotgun analysis of the marine mussel Mytilus edulis hemolymph proteome and mapping the innate immunity elements.. Proteomics, 15(23-24), 4021-4029
Open this publication in new window or tab >>Shotgun analysis of the marine mussel Mytilus edulis hemolymph proteome and mapping the innate immunity elements.
2015 (English)In: Proteomics, ISSN 1615-9853, E-ISSN 1615-9861, Vol. 15, no 23-24, p. 4021-4029Article in journal (Refereed) Published
Abstract [en]

The marine mussel innate immunity provides protection to pathogen invasion and inflammation.In this regard, the mussel hemolymph takes a main role in the animal innate response.Despite the importance of this body fluid in determining the physiological condition of theanimal, little is known about the molecular mechanisms underlying the cellular and humoralresponses. In this work, we have applied aMS (nano-LC-MS/MS) strategy integrating genomicand transcriptomic data with the aim to: (i) identify the main protein functional groups thatcharacterize hemolymph and (ii) to map the elements of innate immunity in the marine musselMytilus edulis hemolymph proteome. After sample analysis and first protein identificationbased onMS/MS data comparison, proteins with unknown functions were annotated with blastusing public database (nrNCBI) information. Overall 595 hemolymph proteins were identifiedwith high confidence and annotated. These proteins encompass primary cellular metabolicprocesses: energy production and metabolism of biomolecules, as well as processes related tooxidative stress defence, xenobiotic detoxification, drug metabolism, and immune response.A group of proteins was identified with putative immune effector, receptor, and signalingfunctions in M. edulis. Data are available via ProteomeXchange with identifier PXD001951(http://proteomecentral.proteomexchange.org/dataset/PXD001951).

Place, publisher, year, edition, pages
Wiley-Blackwell, 2015
Keywords
Animal proteomics / Biomarker / Hemolymph / Innate immunity / Mytilus edulis / Shotgun proteomics
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:liu:diva-123354 (URN)10.1002/pmic.201500118 (DOI)000367728000010 ()26351202 (PubMedID)
Available from: 2015-12-12 Created: 2015-12-12 Last updated: 2017-12-01Bibliographically approved
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