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Abbey-Lee, Robin N.
Publications (4 of 4) Show all publications
Abbey-Lee, R. N., Kreshchenko, A., Fernandez Sala, X., Petkova, I. & Løvlie, H. (2019). Effects of monoamine manipulations on the personality and gene expression of three-spined sticklebacks. Journal of Experimental Biology, 222(20), Article ID jeb211888.
Open this publication in new window or tab >>Effects of monoamine manipulations on the personality and gene expression of three-spined sticklebacks
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2019 (English)In: Journal of Experimental Biology, ISSN 0022-0949, E-ISSN 1477-9145, Vol. 222, no 20, article id jeb211888Article in journal (Refereed) Published
Abstract [en]

Among-individual behavioral differences (i.e. animal personality) are commonly observed across taxa, although the underlying, causal mechanisms of such differences are poorly understood. Animal personality has been correlated with physiological functions as well as fitness-related traits. Variation in many aspects of monoamine systems, such as metabolite levels and gene polymorphisms, has been linked to behavioral variation. Therefore, here we experimentally investigated the potential role of monoamines in explaining individual variation in personality, using two common pharmaceuticals that respectively alter the levels of serotonin and dopamine in the brain: fluoxetine and ropinirole. We exposed three-spined sticklebacks, a species that shows animal personality, to either chemical alone or to a combination of the two chemicals, for 18 days. During the experiment, fish were assayed at four time points for the following personality traits: exploration, boldness, aggression and sociability. To quantify brain gene expression on short- and longer-term scales, fish were sampled at two time points. Our results show that monoamine manipulations influence fish behavior. Specifically, fish exposed to either fluoxetine or ropinirole were significantly bolder, and fish exposed to the two chemicals together tended to be bolder than control fish. Our monoamine manipulations did not alter the gene expression of monoamine or stress-associated neurotransmitter genes, but control, untreated fish showed covariation between gene expression and behavior. Specifically, exploration and boldness were predicted by genes in the dopaminergic, serotonergic and stress pathways, and sociability was predicted by genes in the dopaminergic and stress pathways. These results add further support to the links between monoaminergic systems and personality, and show that exposure to monoamines can causally alter animal personality.

Place, publisher, year, edition, pages
The Company of Biologists Ltd, 2019
Keywords
Animal behavior, Cocktail effects, Dopamine, Ecotoxicology, Fish, Serotonin
National Category
Other Biological Topics
Identifiers
urn:nbn:se:liu:diva-161075 (URN)10.1242/jeb.211888 (DOI)31619541 (PubMedID)2-s2.0-85073434317 (Scopus ID)
Available from: 2019-10-21 Created: 2019-10-21 Last updated: 2019-10-28Bibliographically approved
Abbey-Lee, R. N., Kreshchenko, A., Fernandez Sala, X., Petkova, I. & Løvlie, H. (2019). Effects of monoamine manipulations on the personality and gene expression of three-spined sticklebacks. Cambridge
Open this publication in new window or tab >>Effects of monoamine manipulations on the personality and gene expression of three-spined sticklebacks
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2019 (English)Data set
Abstract [en]

Among-individual behavioral differences (i.e. animal personality) are commonly observed across taxa, although the underlying, causal mechanisms of such differences are poorly understood. Animal personality has been implicated in correlations with physiological functions as well as affecting fitness-related traits. Variation in many aspects of monoamine systems, such as metabolite levels and gene polymorphisms, has been linked to behavioral variation. Therefore, here we investigated the potential role of monoamines in explaining individual variation in personality, using two common pharmaceuticals that respectively alter the levels of serotonin and dopamine in the brain: fluoxetine and ropinirole. We exposed three- spined sticklebacks, a species that shows animal personality, to either chemical alone or to a combination of the two chemicals, for 18 days. During the experiment, fish were assayed at four time points for the following personality traits: exploration, boldness, aggression and sociability. To quantify brain gene expression on short- and longer-term scales, fish were sampled at two time points. Our results show that monoamine manipulations influence fish behavior. Specifically, fish exposed to either fluoxetine or ropinirole were significantly bolder, and fish exposed to the two chemicals together tended to be bolder than control fish. Our monoamine manipulations did not alter the gene expression of monoamine or stress-associated neurotransmitter genes, but control, untreated fish showed covariation between gene expression and behavior. Specifically, exploration and boldness were predicted by genes in the dopaminergic, serotonergic and stress pathways, and sociability was predicted by genes in the dopaminergic and stress pathways. These results add further support to the links between monoaminergic systems and personality, and show that exposure to monoamines can causally alter animal personality.

Place, publisher, year
Cambridge: , 2019
Keywords
animal behavior, cocktail effects, dopamine, ecotoxicology, fish, serotonin
National Category
Behavioral Sciences Biology
Identifiers
urn:nbn:se:liu:diva-160555 (URN)
Available from: 2019-09-27 Created: 2019-09-27 Last updated: 2019-10-28Bibliographically approved
Abbey-Lee, R. N., Uhrig, E., Zidar, J., Favati, A., Almberg, J., Dahlbom, J., . . . Løvlie, H. (2018). The Influence of Rearing on Behavior, Brain Monoamines, and Gene Expression in Three-Spined Sticklebacks. Brain, behavior, and evolution, 91(4), 201-213
Open this publication in new window or tab >>The Influence of Rearing on Behavior, Brain Monoamines, and Gene Expression in Three-Spined Sticklebacks
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2018 (English)In: Brain, behavior, and evolution, ISSN 0006-8977, E-ISSN 1421-9743, Vol. 91, no 4, p. 201-213Article in journal (Refereed) Published
Abstract [en]

The causes of individual variation in behavior are often not well understood, and potential underlying mechanisms include both intrinsic and extrinsic factors, such as early environmental, physiological, and genetic differences. In an exploratory laboratory study, we raised three-spined sticklebacks <i>(Gasterosteus aculeatus)</i> under 4 different environmental conditions (simulated predator environment, complex environment, variable social environment, and control). We investigated how these manipulations related to behavior, brain physiology, and gene expression later in life, with focus on brain dopamine and serotonin levels, turnover rates, and gene expression. The different rearing environments influenced behavior and gene expression, but did not alter monoamine levels or metabolites. Specifically, compared to control fish, fish exposed to a simulated predator environment tended to be less aggressive, more exploratory, and more neophobic; and fish raised in both complex and variable social environments tended to be less neophobic. Exposure to a simulated predator environment tended to lower expression of dopamine receptor DRD4A, a complex environment increased expression of dopamine receptor DRD1B, while a variable social environment tended to increase serotonin receptor 5-HTR2B and serotonin transporter SLC6A4A expression. Despite both behavior and gene expression varying with early environment, there was no evidence that gene expression mediated the relationship between early environment and behavior. Our results confirm that environmental conditions early in life can affect phenotypic variation. However, the mechanistic pathway of the monoaminergic systems translating early environmental variation into observed behavioral responses was not detected.

Place, publisher, year, edition, pages
S. Karger, 2018
Keywords
Dopamine; FishNovel arena; Novel object; Personality; Serotonin
National Category
Biological Sciences
Identifiers
urn:nbn:se:liu:diva-150623 (URN)10.1159/000489942 (DOI)000443740100002 ()29961048 (PubMedID)2-s2.0-85049396090 (Scopus ID)
Note

Funding agencies: Langmanska Kulturfonden; Royal Physiographic Society of Lund; LiU program "Future research leaders"; Center for Systems Neurobiology; Lars Hierta Memorial Foundation

Available from: 2018-08-30 Created: 2018-08-30 Last updated: 2018-09-25Bibliographically approved
Abbey-Lee, R. N., Uhrig, E. J., Zidar, J., Favati, A., Almberg, J., Dahlblom, J., . . . Løvlie, H. (2018). The influence of rearing on behavior, brain monoamines and gene expression in three-spined sticklebacks. Basel: S. Karger
Open this publication in new window or tab >>The influence of rearing on behavior, brain monoamines and gene expression in three-spined sticklebacks
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2018 (English)Data set
Abstract [en]
  1. The causes of individual variation in behavior are often not well understood, and potential underlying mechanisms include both intrinsic and extrinsic factors, such as early environmental, physiological, and genetic differences.
  2. In an exploratory laboratory study, we raised three-spined sticklebacks (Gasterosteus aculeatus) under 4 different environmental conditions (simulated predator environment, complex environment, variable social environment, and control). We investigated how these manipulations related to behavior, brain physiology and gene expression later in life, with focus on brain dopamine and serotonin levels, turnover rates, and gene expression.
  3. The different rearing environments influenced behavior and gene expression, but did not alter monoamine levels or metabolites. Specifically, compared to control fish, fish exposed to a simulated predator environment tended to be less aggressive, more exploratory, and more neophobic; and fish raised in both complex and variable social environments tended to be less neophobic. Exposure to a simulated predator environment tended to lower expression of dopamine receptor DRD4A, a complex environment increased expression of dopamine receptor DRD1B, while a variable social environment tended to increase serotonin receptor 5-HTR2B and increased serotonin transporter SLC6A4A expression. Despite both behavior and gene expression varying with early environment, there was no evidence that gene expression mediated the relationship between early environment and behavior.
  4. Our results confirm that environmental conditions early in life can affect phenotypic variation. However, the mechanistic pathway of the monoaminergic systems translating early environmental variation into observed behavioral responses was not detected.
Place, publisher, year
Basel: S. Karger, 2018
National Category
Biological Sciences
Identifiers
urn:nbn:se:liu:diva-148013 (URN)10.1159/000489942 (DOI)
Available from: 2018-05-24 Created: 2018-05-24 Last updated: 2018-09-25Bibliographically approved
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