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Razmi, N. (2023). (Nano)materials for electrochemical sensing applications in different fields. (Doctoral dissertation). Linköping: Linköping University Electronic Press
Open this publication in new window or tab >>(Nano)materials for electrochemical sensing applications in different fields
2023 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Health care and the medical industry, environmental monitoring and food sector are just a few examples of human’s major concerns. Design and analytical applications of (bio)sensors and sensing (nano)materials in these fields which have drawn tremendous attention by scientific community, require multidisciplinary knowledge in materials, transducers, and measurement methodologies. Among different types of transducers, electrochemical transducers have gained a lot of interest due to their simplicity, ease of fabrication and integration, selectivity, and sensitivity. Application of sensing materials, in particular nanomaterials, provide several improvements for analytical sensing such as selectivity, sensitivity, robustness, and fast response. Therefore, different nanomaterials due to their excellent characterization are exploited as key instruments for the design of high-performant analytical assays in various fields.

Considering this established nanomaterial-based electrochemical sensing applications as alternatives to conventional methods in various fields, in this thesis, we synthesized and characterized some (nano)materials such as gold nanostars (AuNSs), iron oxide/zinc oxide (α-Fe2O3/ZnO) nanocomposite for sensing applications in environmental monitoring. We fabricated screen printed Ruthenium (IV) oxide (RuO2-Nf) pH electrodes and applied them for continuous pH monitoring in milk and during milk coagulation. Although the results indicated the applicability of the RuO2-Nf electrodes for continuous pH measurement in milk as a replacement of traditional glass electrode, they are not suitable for more viscous samples. Later we investigated the cleaning procedure for these pH electrodes, and we showed that soaking them in the solution of 5% pepsin in 0.1 M HCl restores the electrodes to their initial state for pH measurement in food samples. In another work we developed a molecular imprinted polymer-based biosensor for successful detection of prostate-specific antigen. At the end we investigated the effect of pH on the growth of pathogenic bacteria using electrical impedance spectroscopy.

Abstract [sv]

Hälso- och sjukvårdsbranschen, miljöövervakning och livsmedelssektorn är bara några exempel på mänsklighetens stora bekymmer. Design och analytiska tillämpningar av (bio)sensorer och sensormaterial inom dessa områden, som har lockat enorm uppmärksamhet från vetenskapssamhället, kräver tvärvetenskaplig kunskap inom material, omvandlare och mätmetodik. Bland olika typer av omvandlare har elektrokemiska (bio)sensorer väckt stort intresse på grund av sin enkelhet, lätthet att tillverka, selektivitet och känslighet. Användning av nya sensormaterial, särskilt nanomaterial, ger flera förbättringar för analytisk detektion, som exempelvis selektivitet, känslighet, robusthet och snabb respons. Därför utnyttjas olika nanomaterial på grund av deras utmärkta egenskaper som nyckelkomponenter för designen av högpresterande analytiska metoder inom olika områden.

Med tanke på att nanomaterialbaserade elektrokemiska sensorapplikationer är ett hett ämne har vi i denna avhandling syntetiserat och karaktäriserat vissa nanomaterial såsom guld nanostjärnor (AuNSs) och järnoxid/zinkoxid (α-Fe2O3/ZnO) nanokomposit för sensormaterial i miljöövervakning. Vi tillverkade tryckta pH-elektroder av rutenium (IV)oxid (RuO2-Nf) och använde dem för kontinuerlig pH-övervakning i mjölk, och under mjölkkoagulering. Även om resultaten indikerade att RuO2-Nf-elektroderna är användbara för kontinuerlig pH-mätning i mjölk som en ersättning för traditionella glaselektroder, är de inte lämpliga för mer viskösa prov. Senare undersökte vi rengöringsmetoden för dessa pH-elektroder och visade att blötläggning i en lösning av 5% pepsin i 0,1 M HCI återställer elektroderna till sitt ursprungliga tillstånd för pH-mätning i livsmedelsprover. I ett annat arbete utvecklade vi en molekylär imprintingpolymerbaserad biosensor för framgångsrik detektion av prostata-specifikt antigen. Till slut undersökte vi effekten av pH på tillväxten av patogena bakterier med hjälp av elektrisk impedansspektroskopi.

Place, publisher, year, edition, pages
Linköping: Linköping University Electronic Press, 2023. p. 47
Series
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 2336
Keywords
Electrochemical (bio)sensors, Nanomaterials, AuNSs, (α-Fe2O3/ZnO) nanocomposite, (RuO2-Nf) pH electrodes, MIP technology
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:liu:diva-199206 (URN)10.3384/9789180752985 (DOI)9789180752978 (ISBN)9789180752985 (ISBN)
Public defence
2023-10-25, K1, Kåkenhus, Campus Norrköping, Norrköping, 09:30 (English)
Opponent
Supervisors
Note

Funding agency: The European Commission through the Marie Skłodowska-Curie Actions program

Available from: 2023-11-17 Created: 2023-11-17 Last updated: 2024-05-08Bibliographically approved
Razmi, N., Hasanzadeh, M., Willander, M. & Nur, O. (2022). Electrochemical genosensor based on gold nanostars for the detection of Escherichia coli O157:H7 DNA. Analytical Methods, 14(16), 1562-1570
Open this publication in new window or tab >>Electrochemical genosensor based on gold nanostars for the detection of Escherichia coli O157:H7 DNA
2022 (English)In: Analytical Methods, ISSN 1759-9660, E-ISSN 1759-9679, Vol. 14, no 16, p. 1562-1570Article in journal (Refereed) Published
Abstract [en]

Escherichia coli O157:H7 (E. coli O157:H7) is an enterohemorrhagic E. coli (EHEC), which has been issued as a major threat to public health worldwide due to fatal contamination of water and food. Thus, its rapid and accurate detection has tremendous importance in environmental monitoring and human health. In this regard, we report a simple and sensitive electrochemical DNA biosensor by targeting Z3276 as a genetic marker in river water. The surface of the designed gold electrode was functionalized with gold nanostars and an aminated specific sensing probe of E. coli O157:H7 to fabricate the genosensor. Cyclic voltammetry (CV) and square wave voltammetry (SWV) techniques were applied for electrochemical characterization and detection. The synthesized gold nanostars were characterized using different characterization techniques. The fabricated DNA-based sensor exhibited a high selective ability for one, two, and three-base mismatched sequences. Regeneration, stability, selectivity, and kinetics of the bioassay were investigated. Under optimal conditions, the fabricated genosensor exhibited a linear response range of 10(-5) to 10(-17) mu M in the standard sample and 7.3 to 1 x 10(-17) mu M in water samples with a low limit of quantification of 0.01 zM in water samples. The detection strategy based on silver plated gold nanostars and DNA hybridization improved the sensitivity and specificity of the assay for E. coli O157:H7 detection in real water samples without filtration. The detection assay has the advantages of high selectivity, sensitivity, low amounts of reagents, short analysis time, commercialization, and potential application for the determination of other pathogenic bacteria.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2022
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:liu:diva-184389 (URN)10.1039/d2ay00056c (DOI)000776261100001 ()35357389 (PubMedID)
Note

Funding Agencies|European UnionEuropean Commission [H2020-MSCA-ITN-2018-813680]

Available from: 2022-04-22 Created: 2022-04-22 Last updated: 2023-05-04Bibliographically approved
Mehri, P., Pashazadeh-Panahi, P., Hasanzadeh, M. & Razmi, N. (2020). An innovative genosensor for the monitoring of Leishmania spp sequence using binding of pDNA to cDNA based on Cit-AgNPs. Heliyon, 6(8), Article ID e04638.
Open this publication in new window or tab >>An innovative genosensor for the monitoring of Leishmania spp sequence using binding of pDNA to cDNA based on Cit-AgNPs
2020 (English)In: Heliyon, E-ISSN 2405-8440, Vol. 6, no 8, article id e04638Article in journal (Refereed) Published
Abstract [en]

Leishmaniasis considered as the most crucial epidemic-prone diseases according to the World Health Organization. Early diagnoses and therapy of Leishmania infection is a great challenge since, it has no symptom and is resistance to drugs. Therefore, there is an urgent need for sensitive and precise detection of this pathogen. In this study, a new method was developed for optical biosensing of Leishmania spp sequence based on hybridization of Citrate capped Ag nanoparticles bonded to specific single stranded DNA probe of Leishmania spp. Aggregation of the Citrate capped Ag nanoparticles in the existence or lack of a cDNA sequence of Leishmania, cause eye catching and considerable significant alter in the UV–vis. The obtained low limit of quantification (LLOQ) of was achieved as 1ZM. Based on experimental results in optimum conditions, quick bioanalysis of Leishmania spp sequence was performed (2 min). So, this probe can be used for the clinical diagnosis of this pathogen and infection disease. © 2020 The Author(s) Analytical chemistry; Nanotechnology, Nanostructure; Affinity binding; Leishmaniasis; Spectrophotometer; Spectrofluorimetric; Biosensing © 2020 The Author(s)

Place, publisher, year, edition, pages
Elsevier, 2020
Keywords
Analytical chemistry; Nanotechnology; Nanostructure; Affinity binding; Leishmaniasis; Spectrophotometer; Spectrofluorimetric; Biosensing
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:liu:diva-168896 (URN)10.1016/j.heliyon.2020.e04638 (DOI)000568755600008 ()2-s2.0-85089476961 (Scopus ID)
Note

Funding agencies:  Tabriz University of Medical Sciences

Available from: 2020-09-02 Created: 2020-09-02 Last updated: 2020-12-15
Abbasy, L., Mohammadzadeh, A., Hasanzadeh, M. & Razmi, N. (2020). Development of a reliable bioanalytical method based on prostate specific antigen trapping on the cavity of molecular imprinted polymer towards sensing of PSA using binding affinity of PSA-MIP receptor: A novel biosensor. Journal of Pharmaceutical and Biomedical Analysis, 188, Article ID 113447.
Open this publication in new window or tab >>Development of a reliable bioanalytical method based on prostate specific antigen trapping on the cavity of molecular imprinted polymer towards sensing of PSA using binding affinity of PSA-MIP receptor: A novel biosensor
2020 (English)In: Journal of Pharmaceutical and Biomedical Analysis, ISSN 0731-7085, E-ISSN 1873-264X, Vol. 188, article id 113447Article in journal (Refereed) Published
Abstract [en]

In this study, electrically-conducting poly [Toluidine Blue (PTB)] was applied as artificial receptor. It was organized by molecular imprinting approaches and via electrochemical technique for the sensitive monitoring of prostate-specific antigen (PSA). The protein-imprinted PTB was electropolymerized in a pre-formed glutaraldehyde-cysteamine (GA-Cys A) matrix on the surface of gold electrode, which significantly boosted the stability against degradation of the Molecular Imprinted Polymer (MIP) on the surface of pre-modified gold electrode. Moreover, the MIP bio-receptor ability towards protein recognition was explored by some electrochemical techniques. The binding affinity of MIP system was considerably upper than that of non-imprinted polymer (NIP) system, indicating the success of the method in generating imprinted materials that was specifically use to PSA protein. The incubation of the MIP modified electrode in various concentration of PSA (from 1-60 μg/L) resulted in the increase of the Fe (CN)63-/4- redox peak current. The bio-device also showed linear response from 1-60 μg/L and LLOQ of 1 μg/L by using DPV technique, leading to PSA monitoring in clinical samples. The proposed MIP-based biosensor was satisfactorily applied to the determination of PSA in human plasma samples. Therefore, the developed bio-device provides a new approach for sensitive, simple, rapid, and cost-effective monitoring of 1 μg/L of PSA. Notably, this approach could appear as an appropriate candidate for point-of-care (POC) use in clinical and biomedical analyses.

Place, publisher, year, edition, pages
Elsevier, 2020
Keywords
Binding affinity, Biomacromolecules, Biomedical analysis, Biosensor, Encapsulation, Prostate cancer.
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:liu:diva-168893 (URN)10.1016/j.jpba.2020.113447 (DOI)000556785200040 ()32623317 (PubMedID)
Note

Funding agencies:  Tabriz University of Medical Sciences

Available from: 2020-09-02 Created: 2020-09-02 Last updated: 2023-11-17
Bahavarnia, F., Pashazadeh-Panahi, P., Hasanzadeh, M. & Razmi, N. (2020). DNA based biosensing of Acinetobacter baumannii using nanoparticles aggregation method. Heliyon, 6(7), Article ID e04474.
Open this publication in new window or tab >>DNA based biosensing of Acinetobacter baumannii using nanoparticles aggregation method
2020 (English)In: Heliyon, E-ISSN 2405-8440, Vol. 6, no 7, article id e04474Article in journal (Refereed) Published
Abstract [en]

Acinetobacter baumannii is the main cause of nosocomial infections in blood, urinary tract, wounds and in lungs leading to pneumonia. Apart from its strong predilection to be the cause of serious illnesses in intensive care units. Herein, we present a specific and sensitive approach for the monitoring of Acinetobacter baumannii genome based on citrate capped silver nanoparticles (Cit-AgNPs) using spectroscopic methods. In this study, (5' SH-TTG TGA ACT ATT TAC GTC AGC ATG C3') sequence was used as a probe DNA (pDNA) of Acinetobacter baumannii. Then, complementary DNA (cDNA) was used for hybridization. After the hybridization of pDNA with cDNA, target DNA (5' GCA TGC TGA CGT AAA TAGTTC ACA A 3') was recognized and detected using turn-on fluorescence bioassay. After the hybridization of pDNA with cDNA, the target DNA was successfully measured in optimum time of 2 min by spectrophotometric techniques. Moreover, the selectivity of designed bioassay was evaluated in the presence of two mismatch sequences and excellent differentiation was obtained. 1 Zepto-molar (zM) of low limit of quantification (LLOQ) was achieved by this genosensor. The present study paved the way for quick (2 min) and accurate detection of Acinetobacter baumannii, which can be a good alternative to the traditional methods. Current study proposed a novel and significant diagnostic test towards Acinetobacter baumannii detection based on silver nanoparticles aggregation which has the capability of being a good alternative to the traditional methods. Moreover, the proposed genosensor successfully could be applied for the detection of other pathogens.

Place, publisher, year, edition, pages
ELSEVIER, 2020
Keywords
Chemistry, Microbiology, Nanoparticles, Analytical chemistry, Sensor, Biosensor, Genosensor, Acinetobacter baumannii, DNA hybridization, Pathogenic bacteria
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:liu:diva-168897 (URN)10.1016/j.heliyon.2020.e04474 (DOI)000558743200014 ()32695920 (PubMedID)
Note

Funding Agency: Tabriz University of Medical Sciences 

Available from: 2020-09-02 Created: 2020-09-02 Last updated: 2020-12-15
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0002-1829-8138

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