liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Evaluation of novel fluorescent probes for in vivo Transthyretin amyloid using fibrils generated in vitro under varying conditions
Linköping University, Department of Physics, Chemistry and Biology. (Hammarström Lab)
2019 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Transthyretin (TTR) amyloidosis is a disease that appears in three variants. One variant affects the elderly population with heart failure, the other two variants are hereditary and caused by an amino acid substitution in the gene, resulting in polyneuropathy and/or heart issues depending on the amino acid substitution. However, in all three variants, other organs may also be affected with amyloid deposition in the disease course. Amyloid fibrils of TTR (ATTR) contains a mixture of full-length protein and fragments (50-127).

Luminescent conjugated oligothiophenes (LCO’s) are novel amyloid binding probes used to stain amyloid fibrils and these amyloid probes have the feature of characterizing the amyloid structure in terms of fluorescence spectra. Apart from LCO’s, a few other amyloid binding probes are used to stain recombinant amyloid transthyretin and native transthyretin for binding studies. The majority of generated TTR aggregates in vitro did not have the characteristic fluorescence spectra when bound to LCO’s and was observed as a clumped gel-like aggregate. The generation of recombinant TTR fibrils in vitro using the mutant TTR-T49M to obtain an aggregation prone fragment (50-127) after being treated with cyanogen bromide had a low yield of in vivo amyloid-like fibrils, but with characteristic LCO spectra. Carpal tunnel ATTR often precedes ATTR deposition in heart tissue. Amyloid transthyretin in carpal tunnel tissues was stained with LCO’s and used as a reference in the comparison against the in vitro generated recombinant amyloid transthyretin fibrils. This project also includes quantification of amyloid transthyretin in a few selected parts of the carpal tunnel tissue using ImageJ. In the long run this method could help in diagnosing TTR amyloidosis.

Place, publisher, year, edition, pages
2019. , p. 50
Keywords [en]
Transthyretin amyloidosis, Luminescent conjugated oligothiophene, Recombinant transthyretin fibrils, TTR-T49M, Cyanogen bromide, Fluorescence spectra
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:liu:diva-154611ISRN: LITH-IFM-A-EX--19/3598--SEOAI: oai:DiVA.org:liu-154611DiVA, id: diva2:1290828
Subject / course
Chemical Biology
Presentation
2019-02-01, Bikupan, Linköpings universitet, Linköping, 11:15 (English)
Supervisors
Examiners
Available from: 2019-02-21 Created: 2019-02-21 Last updated: 2019-02-21Bibliographically approved

Open Access in DiVA

fulltext(4610 kB)47 downloads
File information
File name FULLTEXT01.pdfFile size 4610 kBChecksum SHA-512
7baa36aa756a673cc1bfa554218cfe0e464a45ad3296a094a8944ca8815bc0fe36a31d4a9b26304c613057959ee9dd9adbe6364521e7004d8406d88250e40ede
Type fulltextMimetype application/pdf

Search in DiVA

By author/editor
Duong, Sun
By organisation
Department of Physics, Chemistry and Biology
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 47 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

urn-nbn

Altmetric score

urn-nbn
Total: 312 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf