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New Methods for Selecting Stallion Spermatozoa for Assisted Reproduction
AVANTEA.
AVANTEA.
AVANTEA.
Linköpings universitet, Hälsouniversitetet. Linköpings universitet, Institutionen för klinisk och experimentell medicin, Medicinsk genetik.ORCID-id: 0000-0002-5194-2124
Vise andre og tillknytning
2011 (engelsk)Inngår i: Journal of Equine Veterinary Science, ISSN 0737-0806, E-ISSN 1542-7412, Vol. 31, nr 9, s. 536-541Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Improved sperm selection techniques are needed to increase the efficiency of equine-assisted reproduction. Single layer centrifugation (SLC) of spermatozoa has been shown to improve the quality of stallion sperm samples. In this study, the functionality of selected stallion spermatozoa was tested by intracytoplasmic sperm injection of equine oocytes after selection by SLC through Androcoll-E or by discontinuous density gradient centrifugation (DGC) through Redigrad and Tyrodes medium with added albumin, lactate, and pyruvate. The mean cleavage rates of the injected oocytes from SLC- and DGC-selected spermatozoa were 67% and 66%, respectively, whereas the proportion of blastocysts developing from cleaved oocytes was 28% and 22%, respectively (P andgt; .05, not significant). An incidental finding was that there was a tendency for SLC-selected spermatozoa to have a higher percentage of spermatozoa with normal morphology than DGC (70% +/- 22% vs. 58% +/- 38%) and for more blastocysts to be obtained from subfertile ejaculates (21 [19.6%] vs. 15 [14.4%], respectively). In further experiments, stallion spermatozoa bound to hyaluronan, although binding may depend on the semen extender and sperm treatment as well as incubation time. In conclusion, SLC-selected stallion spermatozoa function normally when injected into oocytes. SLC may potentially be better than DGC at selecting spermatozoa from subfertile ejaculates, but this effect needs rigorous investigation with a much larger sample size. Use of the hyaluronan-binding assay for assessing the potential fertility of stallion spermatozoa may be useful but requires further evaluation.

sted, utgiver, år, opplag, sider
WB Saunders , 2011. Vol. 31, nr 9, s. 536-541
Emneord [en]
ICSI, Stallion spermatozoa, Hyaluronan binding, Androcoll-E, Single layer centrifugation
HSV kategori
Identifikatorer
URN: urn:nbn:se:liu:diva-71386DOI: 10.1016/j.jevs.2011.03.009ISI: 000295117300008OAI: oai:DiVA.org:liu-71386DiVA, id: diva2:448047
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|Tilgjengelig fra: 2011-10-14 Laget: 2011-10-14 Sist oppdatert: 2017-12-08

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Rodriguez-Martinez, Heriberto

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