liu.seSearch for publications in DiVA
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Tissue Harvesting Site and Culture Medium Affect Attachment, Growth, and Phenotype of Ex Vivo Expanded Oral Mucosal Epithelial Cells
Harvard Medical Sch, MA USA; University of Oslo, Norway; Oslo University Hospital, Norway.
Oslo University Hospital, Norway.
Harvard Medical Sch, MA USA; University of Coll Southeast Norway, Norway; Innlandet Hospital Trust, Norway.
Harvard Medical Sch, MA USA; Oslo University Hospital, Norway; University of Oslo, Norway.
Vise andre og tillknytning
2017 (engelsk)Inngår i: Scientific Reports, E-ISSN 2045-2322, Vol. 7, artikkel-id 674Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Transplantation of cultured oral mucosal epithelial cells (OMECs) is a promising treatment strategy for limbal stem cell deficiency. In order to improve the culture method, we investigated the effects of four culture media and tissue harvesting sites on explant attachment, growth, and phenotype of OMECs cultured from Sprague-Dawley rats. Neither choice of media or harvesting site impacted the ability of the explants to attach to the culture well. Dulbeccos modified Eagles medium/Hams F12 (DMEM) and Roswell Park Memorial Institute 1640 medium (RPMI) supported the largest cellular outgrowth. Fold outgrowth was superior from LL explants compared to explants from the buccal mucosa (BM), HP, and transition zone of the lower lip (TZ) after six-day culture. Putative stem cell markers were detected in cultures grown in DMEM and RPMI. In DMEM, cells from TZ showed higher colony-forming efficiency than LL, BM, and HP. In contrast to RPMI, DMEM both expressed the putative stem cell marker Bmi-1 and yielded cell colonies. Our data suggest that OMECs from LL and TZ cultured in DMEM give rise to undifferentiated cells with high growth capacity, and hence are the most promising for treatment of limbal stem cell deficiency.

sted, utgiver, år, opplag, sider
NATURE PUBLISHING GROUP , 2017. Vol. 7, artikkel-id 674
HSV kategori
Identifikatorer
URN: urn:nbn:se:liu:diva-136861DOI: 10.1038/s41598-017-00417-zISI: 000398545400018PubMedID: 28386069OAI: oai:DiVA.org:liu-136861DiVA, id: diva2:1092111
Tilgjengelig fra: 2017-04-30 Laget: 2017-04-30 Sist oppdatert: 2022-09-15

Open Access i DiVA

fulltext(3578 kB)261 nedlastinger
Filinformasjon
Fil FULLTEXT01.pdfFilstørrelse 3578 kBChecksum SHA-512
7500c8bdbfe09178e4beeacc686facaff97604ec041a22782ebf215c47481fbbc2b583e52e2bfa55a7cf9b4a944b4560dd8801f656abfa2b8bcf3f2d421a91eb
Type fulltextMimetype application/pdf

Andre lenker

Forlagets fulltekstPubMed

Søk i DiVA

Av forfatter/redaktør
Griffith, May
Av organisasjonen
I samme tidsskrift
Scientific Reports

Søk utenfor DiVA

GoogleGoogle Scholar
Totalt: 261 nedlastinger
Antall nedlastinger er summen av alle nedlastinger av alle fulltekster. Det kan for eksempel være tidligere versjoner som er ikke lenger tilgjengelige

doi
pubmed
urn-nbn

Altmetric

doi
pubmed
urn-nbn
Totalt: 175 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf