liu.seSearch for publications in DiVA
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Exogenous Individual Lecithin-Phospholipids (Phosphatidylcholine and Phosphatidylglycerol) Cannot Prevent the Oxidative Stress Imposed by Cryopreservation of Boar Sperm.
Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för barns och kvinnors hälsa. Linköpings universitet, Medicinska fakulteten.ORCID-id: 0000-0003-0120-354X
Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för kliniska vetenskaper. Linköpings universitet, Medicinska fakulteten.ORCID-id: 0000-0002-0256-1958
Linköpings universitet, Institutionen för klinisk och experimentell medicin, Avdelningen för barns och kvinnors hälsa. Linköpings universitet, Medicinska fakulteten.
2017 (engelsk)Inngår i: Journal of veterinary medicine and surgery, ISSN 2574-2868, Vol. 1, nr 1Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Objective: Despite the use of high proportions of the chemically undefined lipoprotein/phospholipid-rich egg-yolk in extenders, boar sperm are highly sensitive to cooling, which induces ROS generation and disrupts the plasma membrane.

Here, we studied whether replacement of hen egg-yolk by commercially defined lecithin phospholipids, derived from egg (LPGE: phosphatidyl glycerol, LPCE: phosphatidyl choline) or soybean (LPCS: phosphatidyl choline), could individually ameliorate such oxidative effects during cryopreservation of ejaculated (sperm rich fraction, SRF) or of cauda-epididymal sperm, retrieved post-mortem from the same males.

Methods: A conventional extender (lactose buffer, with 20% egg-yolk, 0.5% OEP and 3% glycerol) was used as control. Cryodamage was assessed as loss of sperm motility, membrane and acrosome intactness, early membrane destabilization changes, mitochondrial potential, superoxide and ROS production, to finally determine lipid peroxidation (LPO) using specific probes.

Results and conclusion: In general, the exogenous phospholipids assayed were unable of maintaining neither sperm motility nor viability post-thaw compared to controls, owing to increased ROS production and lipid peroxidation. In our study, mitochondrial superoxide production resulted in very high levels for all groups, whereas both ROS production and lipid peroxidation were reduced in the control group, containing emulsified hen egg yolk. Further studies using various dosage and combination of LPCS should be followed for their eventual protective effect.

Keywords: Cryodamage; Sperm; Boar; Mitochondrial activation; Mitochondrial superoxide; ROS production; Lipid peroxidation

sted, utgiver, år, opplag, sider
2017. Vol. 1, nr 1
HSV kategori
Identifikatorer
URN: urn:nbn:se:liu:diva-140728OAI: oai:DiVA.org:liu-140728DiVA, id: diva2:1139835
Tilgjengelig fra: 2017-09-08 Laget: 2017-09-08 Sist oppdatert: 2017-09-18

Open Access i DiVA

fulltext(512 kB)62 nedlastinger
Filinformasjon
Fil FULLTEXT01.pdfFilstørrelse 512 kBChecksum SHA-512
5f085a07a2e89c7e70d5599b92421a609e73303019c48cf5513d0c1d2e920a58f1b5c491a070903841ecc3c43db7a9ae788e7ceb1bf864d9b7b4344d3c6c2ed5
Type fulltextMimetype application/pdf

Andre lenker

http://www.imedpub.com/articles/exogenous-individual-lecithinphospholipids-phosphatidylcholine-and-phosphatidylglycerol-cannot-prevent-the-oxidative-stress-impose.pdf

Søk i DiVA

Av forfatter/redaktør
Alvarez-Rodriguez, ManuelVicente-Carrillo, AlejandroRodriguez-Martinez, Heriberto
Av organisasjonen

Søk utenfor DiVA

GoogleGoogle Scholar
Totalt: 62 nedlastinger
Antall nedlastinger er summen av alle nedlastinger av alle fulltekster. Det kan for eksempel være tidligere versjoner som er ikke lenger tilgjengelige

urn-nbn

Altmetric

urn-nbn
Totalt: 651 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf