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RNAi-mediated gene silencing in Rhynchophorus ferrugineus (Oliver) (Coleoptera: Curculionidae)
University of Mediterranea Reggio Calabria, Italy.
University of Mediterranea Reggio Calabria, Italy.
Newcastle University, England.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Biologi. Linköpings universitet, Tekniska fakulteten. University of Mediterranea Reggio Calabria, Italy.ORCID-id: 0000-0002-0811-1229
Vise andre og tillknytning
2017 (engelsk)Inngår i: Open Life Sciences, ISSN 2391-5412, Vol. 12, nr 1, s. 214-222Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

RNA interference (RNAi) is a powerful strategy for gene function analysis, and it is also widely studied in view of a promising use in pest control. The red palm weevil, Rhynchophorus ferrugineus (Oliver) (Coleoptera: Curculionidae), is one of the most devastating pests of palm in the world. Conventional pest management practices are not adequate to control this insect, thus the development of efficient approaches with minimal environmental impact are needed. In this work, the potential of RNAi in R. ferrugineus has been investigated through the silencing of three different genes (alpha-amylase, V-ATPase, Ecdysone receptor). For each gene we tested two different doses (1,500 and 5,500 ng) and two delivery techniques (injection and ingestion), evaluating both gene knockdown and mortality on insects. Results show that RNAi mediated gene silencing in R. ferrugineus varies from gene to gene, and that the response is dose-dependent, with stronger effects when dsRNA was administered by injection. In parallel, the same study was carried out with the model organism Tribolium castaneum Herbst (Coleoptera: Tenebrionidae), with results showing a different pattern of response, although the two insects belong to the same order.

sted, utgiver, år, opplag, sider
De Gruyter Open, 2017. Vol. 12, nr 1, s. 214-222
Emneord [en]
alpha-amylase; Ecdysone receptor; dsRNA; injection; oral delivery; RNA interference; V-ATPase
HSV kategori
Identifikatorer
URN: urn:nbn:se:liu:diva-140980DOI: 10.1515/biol-2017-0025ISI: 000408793400001Scopus ID: 2-s2.0-85028851498OAI: oai:DiVA.org:liu-140980DiVA, id: diva2:1142300
Tilgjengelig fra: 2017-09-19 Laget: 2017-09-19 Sist oppdatert: 2018-04-26bibliografisk kontrollert

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