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Real-time visualizing the regulation of reactive oxygen species on Zn2+ release in cellular lysosome by a specific fluorescent probe
Linköpings universitet, Institutionen för fysik, kemi och biologi, Molekylär ytfysik och nanovetenskap. Linköpings universitet, Tekniska fakulteten.
Anhui Univ, Peoples R China.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Molekylär ytfysik och nanovetenskap. Linköpings universitet, Tekniska fakulteten.
Anhui Univ, Peoples R China.
Vise andre og tillknytning
2018 (engelsk)Inngår i: Sensors and actuators. B, Chemical, ISSN 0925-4005, E-ISSN 1873-3077, Vol. 264, s. 419-425Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Reactive oxygen species (ROS) regulating the release of free zinc ions (Zn2+) in cellular lysosome is closely related to various pathways of cellular signal transduction, such as inflammation and oxidative stress. Directly visualizing Zn2+ release in lysosome is essential for in-depth understanding these physiological processes, and is still an atelic challenge. In this work, we successfully fabricate a lysosome-specific Zn2+ fluorescent probe and achieve the visualization of ROS-induced Zn2+ release in lysosome of inflammatory cells. The as-prepared probe combines a green fluorophore, an ionophore with five-dentate sites, and a morpholine as the lysosome-specific localization moiety. The fluorescence of the fluorophore in the free probe is suppressed by a photoinduced electron transfer (PET) process from nitrogen atoms in the ionophore. Upon the addition of Zn2+, the fluorescence can be promoted immediately, achieving the real-time detection. Meanwhile, the probe is sensitive and selective to Zn2+, which provides the capability to detect low-concentration of free Zn2+ in lysosomes. Accordingly, the Zn2+ release was clearly observed in lysosome with the increase of ROS levels when the inflammation occurred in living cells. (c) 2018 Published by Elsevier B.V.

sted, utgiver, år, opplag, sider
ELSEVIER SCIENCE SA , 2018. Vol. 264, s. 419-425
Emneord [en]
Fluorescent probe; Realtime visualization; Lysosome-specific Zn2+ probe; ROS regulation; Exogenous and endogenous Zn2+
HSV kategori
Identifikatorer
URN: urn:nbn:se:liu:diva-147363DOI: 10.1016/j.snb.2018.02.055ISI: 000428032900050OAI: oai:DiVA.org:liu-147363DiVA, id: diva2:1207073
Merknad

Funding Agencies|Swedish Research Council (VR) [621-2013-5357]; National Natural Science Foundation of China [21705001, 21577098]; Swedish Government strategic faculty grant in material science (SFO, MATLIU) in Advanced Functional Materials (AFM) (VR) [5.1-2015-5959]; Knut and Alice Wallenberg Foundation (KAW) [2012.0083]; Centre in Nanoscience and Nanotechnology (CeNano)

Tilgjengelig fra: 2018-05-18 Laget: 2018-05-18 Sist oppdatert: 2018-06-05

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Fulltekst tilgjengelig fra 2020-03-07 12:11
Tilgjengelig fra 2020-03-07 12:11

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