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Histo-blood group antigen-binding specificities of human rotaviruses are associated with gastroenteritis but not with in vitro infection
Univ Nantes, France.
Univ Nantes, France.
Univ Nantes, France; Univ Nantes, France.
Univ Nantes, France; Univ Nantes, France.
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2018 (engelsk)Inngår i: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 8, artikkel-id 12961Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Human strains of rotavirus A (RVAs) recognize fucosylated glycans belonging to histo-blood group antigens (HBGAs) through their spike protein VP8*. Lack of these ligands due to genetic polymorphisms is associated with resistance to gastroenteritis caused by P[8] genotype RVAs. With the aim to delineate the contribution of HBGAs in the process, we analyzed the glycan specificity of VP8* proteins from various P genotypes. Binding to saliva of VP8* from P[8] and P[4] genotypes required expression of both FUT2 and FUT3 enzymes, whilst binding of VP8* from the P[14] genotype required FUT2 and A enzymes. We further defined a glycan motif, GlcNAc beta 3Gal beta 4GlcNAc, recognized by P[6] clinical strains. Conversion into Lewis antigens by the FUT3 enzyme impaired recognition, explaining their lower binding to saliva of Lewis positive phenotype. In addition, the presence of neutralizing antibodies was associated with the presence of the FUT2 wild type allele in sera from young healthy adults. Nonetheless, in vitro infection of transformed cell lines was independent of HBGAs expression, indicating that HBGAs are not human RV receptors. The match between results from saliva-based binding assays and the epidemiological data indicates that the polymorphism of human HBGAs controls susceptibility to RVAs, although the exact mechanism remains unclear.

sted, utgiver, år, opplag, sider
NATURE PUBLISHING GROUP , 2018. Vol. 8, artikkel-id 12961
HSV kategori
Identifikatorer
URN: urn:nbn:se:liu:diva-151201DOI: 10.1038/s41598-018-31005-4ISI: 000442917500033PubMedID: 30154494OAI: oai:DiVA.org:liu-151201DiVA, id: diva2:1248042
Merknad

Funding Agencies|Agence Nationale de la Recherche (France): GASTROVIM; Region des Pays de la Loire (France): ARMINA; Merieux Research Grant GOMMs; Russian Science Foundation [14-5-00131]; Swedish Research Council [320301]

Tilgjengelig fra: 2018-09-13 Laget: 2018-09-13 Sist oppdatert: 2018-10-15

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