liu.seSearch for publications in DiVA
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Evaluation of novel fluorescent probes for in vivo Transthyretin amyloid using fibrils generated in vitro under varying conditions
Linköpings universitet, Institutionen för fysik, kemi och biologi. (Hammarström Lab)
2019 (engelsk)Independent thesis Advanced level (degree of Master (Two Years)), 20 poäng / 30 hpOppgave
Abstract [en]

Transthyretin (TTR) amyloidosis is a disease that appears in three variants. One variant affects the elderly population with heart failure, the other two variants are hereditary and caused by an amino acid substitution in the gene, resulting in polyneuropathy and/or heart issues depending on the amino acid substitution. However, in all three variants, other organs may also be affected with amyloid deposition in the disease course. Amyloid fibrils of TTR (ATTR) contains a mixture of full-length protein and fragments (50-127).

Luminescent conjugated oligothiophenes (LCO’s) are novel amyloid binding probes used to stain amyloid fibrils and these amyloid probes have the feature of characterizing the amyloid structure in terms of fluorescence spectra. Apart from LCO’s, a few other amyloid binding probes are used to stain recombinant amyloid transthyretin and native transthyretin for binding studies. The majority of generated TTR aggregates in vitro did not have the characteristic fluorescence spectra when bound to LCO’s and was observed as a clumped gel-like aggregate. The generation of recombinant TTR fibrils in vitro using the mutant TTR-T49M to obtain an aggregation prone fragment (50-127) after being treated with cyanogen bromide had a low yield of in vivo amyloid-like fibrils, but with characteristic LCO spectra. Carpal tunnel ATTR often precedes ATTR deposition in heart tissue. Amyloid transthyretin in carpal tunnel tissues was stained with LCO’s and used as a reference in the comparison against the in vitro generated recombinant amyloid transthyretin fibrils. This project also includes quantification of amyloid transthyretin in a few selected parts of the carpal tunnel tissue using ImageJ. In the long run this method could help in diagnosing TTR amyloidosis.

sted, utgiver, år, opplag, sider
2019. , s. 50
Emneord [en]
Transthyretin amyloidosis, Luminescent conjugated oligothiophene, Recombinant transthyretin fibrils, TTR-T49M, Cyanogen bromide, Fluorescence spectra
HSV kategori
Identifikatorer
URN: urn:nbn:se:liu:diva-154611ISRN: LITH-IFM-A-EX--19/3598--SEOAI: oai:DiVA.org:liu-154611DiVA, id: diva2:1290828
Fag / kurs
Chemical Biology
Presentation
2019-02-01, Bikupan, Linköpings universitet, Linköping, 11:15 (engelsk)
Veileder
Examiner
Tilgjengelig fra: 2019-02-21 Laget: 2019-02-21 Sist oppdatert: 2019-02-21bibliografisk kontrollert

Open Access i DiVA

fulltext(4610 kB)47 nedlastinger
Filinformasjon
Fil FULLTEXT01.pdfFilstørrelse 4610 kBChecksum SHA-512
7baa36aa756a673cc1bfa554218cfe0e464a45ad3296a094a8944ca8815bc0fe36a31d4a9b26304c613057959ee9dd9adbe6364521e7004d8406d88250e40ede
Type fulltextMimetype application/pdf

Søk i DiVA

Av forfatter/redaktør
Duong, Sun
Av organisasjonen

Søk utenfor DiVA

GoogleGoogle Scholar
Totalt: 47 nedlastinger
Antall nedlastinger er summen av alle nedlastinger av alle fulltekster. Det kan for eksempel være tidligere versjoner som er ikke lenger tilgjengelige

urn-nbn

Altmetric

urn-nbn
Totalt: 312 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf