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Selective potentiometric determination of uric acid with uricase immobilized on ZnO nanowires
Linköpings universitet, Institutionen för teknik och naturvetenskap. Linköpings universitet, Tekniska högskolan.
Linköpings universitet, Institutionen för teknik och naturvetenskap. Linköpings universitet, Tekniska högskolan.
Linköpings universitet, Institutionen för teknik och naturvetenskap. Linköpings universitet, Tekniska högskolan.
Linköpings universitet, Institutionen för teknik och naturvetenskap. Linköpings universitet, Tekniska högskolan.
Vise andre og tillknytning
2011 (engelsk)Inngår i: Sensors and actuators. B, Chemical, ISSN 0925-4005, E-ISSN 1873-3077, Vol. 152, nr 2, s. 241-247Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

In this study, a potentiometric uric acid biosensor was fabricated by immobilization of uricase onto zinc oxide (ZnO) nanowires. Zinc oxide nanowires with 80-150 nm in diameter and 900 nm to 1.5 mu m in lengths were grown on the surface of a gold coated flexible plastic substrate. Uricase was electrostatically immobilized on the surface of well aligned ZnO nanowires resulting in a sensitive, selective, stable and reproducible uric acid biosensor. The potentiometric response of the ZnO sensor vs Ag/AgCl reference electrode was found to be linear over a relatively wide logarithmic concentration range (1-650 mu M) suitable for human blood serum. By applying a Nafion (R) membrane on the sensor the linear range could be extended to 1-1000 mu M at the expense of an increased response time from 6.25 s to less than 9 s. On the other hand the membrane increased the sensor durability considerably. The sensor response was unaffected by normal concentrations of common interferents such as ascorbic acid, glucose, and urea.

sted, utgiver, år, opplag, sider
Elsevier Science B.V., Amsterdam. , 2011. Vol. 152, nr 2, s. 241-247
Emneord [en]
ZnO nanowires, Potentiometric nanosensor, Uricase, Uric acid, Nafion (R), Membrane, Electrochemical nanodevices
HSV kategori
Identifikatorer
URN: urn:nbn:se:liu:diva-67542DOI: 10.1016/j.snb.2010.12.015ISI: 000288877700015OAI: oai:DiVA.org:liu-67542DiVA, id: diva2:411267
Merknad
Original Publication: Syed Usman Ali, Naveed Ul Hassan Alvi, Zafar Hussain Ibupoto, Omer Nur, Magnus Willander and Bengt Danielsson, Selective potentiometric determination of uric acid with uricase immobilized on ZnO nanowires, 2011, SENSORS AND ACTUATORS B-CHEMICAL, (152), 2, 241-247. http://dx.doi.org/10.1016/j.snb.2010.12.015 Copyright: Elsevier Science B.V., Amsterdam. http://www.elsevier.com/ Tilgjengelig fra: 2011-04-18 Laget: 2011-04-18 Sist oppdatert: 2017-12-11bibliografisk kontrollert
Inngår i avhandling
1. Fabrication and characterization of ZnO nanostructures for sensing and photonic device applications
Åpne denne publikasjonen i ny fane eller vindu >>Fabrication and characterization of ZnO nanostructures for sensing and photonic device applications
2012 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
Abstract [en]

Nanotechnology is an emerging inter-disciplinary paradigm which encompasses diverse fields of science and engineering converge at the nanoscale. This nanoscale science and nanostructure engineering have well demonstrated in the fabrication of sensors/transducers devices with faster response time and better sensitivity then the planer version of the sensor’s configurations. Nanotechnology is not just to grow/fabricate nanostructures by just mixing nanoscale materials together but it requires the ability to understand and to precisely manipulate and control of the developed nanomaterials in a useful way. Nanotechnology is aiding to substantially improve, even revolutionize, many technology and industry sectors like information technology, energy, environmental science, medicine/medical instrumentation, homeland security, food safety, and transportation, among many others. Such applications of nanotechnology are delivering in both expected and unexpected ways on nanotechnology’s promise to benefit the society.

The semiconductor ZnO with wide band gap (~ 3.37 eV) is a distinguish and unique material and its nanostructures have attracted great attention among the researchers due to its peculiar properties such as large exciton binding energy (60 meV) at room temperature, the high electron mobility, high thermal conductivity, good transparency and easiness of fabricating it in the different type of nanostructures. Based on all these fascinating properties, ZnO have been chosen as a suitable material for the fabrication of photonic, transducers/sensors, piezoelectric, transparent and spin electronics devices etc. The objective of the current study is to highlight the recent developments in materials and techniques for electrochemical sensing and hetrostructure light emitting diodes (LEDs) luminescence properties based on the different ZnO nanostructures. The sensor devices fabricated and characterized in the work were applied to determine and monitor the real changes of the chemical or biochemical species. We have successfully demonstrated the application of our fabricated devices as primary transducers/sensors for the determination of extracellular glucose and the glucose inside the human fat cells and frog cells using the potentiometric technique. Moreover, the fabricated ZnO based nanosensors have also been applied for the selective determination of uric acid, urea and metal ions successfully. This thesis relates specifically to zinc oxide nanostructure based electrochemical sensors and photonic device (LED) applications.

sted, utgiver, år, opplag, sider
Linköping: Linköping University Electronic Press, 2012. s. 76
Serie
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 1412
Emneord
Nanotechnology, zinc oxide, nanowires/ nanorods, nanotubes, nanoporous/nanoflakes, electrochemical sensor and photonic devices
HSV kategori
Identifikatorer
urn:nbn:se:liu:diva-72774 (URN)978-91-7393-015-4 (ISBN)
Disputas
2012-01-17, K3, Kåkenhus, Campus Norrköping, Linköpings universitet, Norrköping, 10:15 (engelsk)
Opponent
Veileder
Tilgjengelig fra: 2011-12-07 Laget: 2011-12-07 Sist oppdatert: 2014-01-15bibliografisk kontrollert

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