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Applying a customised DNA polymerase blend in forensic DNA profiling
Swedish National Laboratory of Forensic Science, Linköping, Sweden.
Swedish National Laboratory of Forensic Science, Linköping, Sweden.
Swedish National Laboratory of Forensic Science, Linköping, Sweden.
Swedish National Laboratory of Forensic Science, Linköping, Sweden.
Vise andre og tillknytning
2011 (engelsk)Inngår i: Book of Abstracts, 2011, s. 161-Konferansepaper, Publicerat paper (Fagfellevurdert)
Abstract [en]

Crime scene stains often contain extraneous compounds that may interfere with PCR-based DNA analysis,resulting in partial or negative/blank DNA profiles. Extensive DNA purification may remove PCR inhibitors, butinvolve a risk of DNA loss and introduction of contaminations. Customising the chemical content of the PCRreaction is a strategy that may increase PCR inhibitor tolerance without manipulating the sample. Previously wehave shown that crime scene stain analysis can be significantly improved by replacing the commonly used DNApolymerase AmpliTaq Gold with either individual alternative DNA polymerases or a blend of such enzymes [1,2].Here we present the validation of AmpFℓSTR SGM Plus with a modified PCR chemistry for routine casework,applying a 1:1 blend of the DNA polymerases ExTaq Hot Start and PicoMaxx High Fidelity. Allele callings areidentical to standard analysis, and stutters sizes and balance values are indistinguishable. The modified chemistryprovides increased resistance to PCR inhibitors, resulting in an elevated number of detected alleles for crimescene stains of both blood and secretion/saliva. Additionally, the detection limit is improved.[1] Hedman, J., Nordgaard, A., Rasmusson, B., Ansell, R. and Rådström, P. (2009) Improved forensic DNA analysis through the use of alternativeDNA polymerases and statistical modeling of DNA profiles. Biotechniques, 47, 951-958.[2] Hedman, J., Nordgaard, A., Dufva, C., Rasmusson, B., Ansell, R. and Rådström, P. (2010) Synergy between

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2011. s. 161-
HSV kategori
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URN: urn:nbn:se:liu:diva-70275OAI: oai:DiVA.org:liu-70275DiVA, id: diva2:437715
Konferanse
24th World Congress of the International Society for Forensic Genetics 2011
Tilgjengelig fra: 2011-08-30 Laget: 2011-08-30 Sist oppdatert: 2011-09-05

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Totalt: 81 treff
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