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Weak effect of strontium on early implant fixation in rat tibia
Linköpings universitet, Institutionen för fysik, kemi och biologi. Linköpings universitet, Tekniska högskolan.
Linköpings universitet, Institutionen för klinisk och experimentell medicin, Ortopedi och idrottsmedicin. Linköpings universitet, Hälsouniversitetet.
Linköpings universitet, Institutionen för klinisk och experimentell medicin, Ortopedi och idrottsmedicin. Linköpings universitet, Hälsouniversitetet.
Turku Clinical Biomaterial Centre, The University of Turku, FI-20520 Turku, Finland.
Vise andre og tillknytning
2012 (engelsk)Inngår i: Bone, ISSN 8756-3282, E-ISSN 1873-2763, Vol. 50, nr 1, s. 350-356Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Strontium ranelate increases bone mass and is used in the treatment of osteoporosis. Its effects in metaphyseal bone repair are largely unknown. We inserted a stainless steel and a PMMA screw into each tibia of male Sprague-Dawley rats. The animals were fed with ordinary feed (n =40) or with addition of strontium ranelate (800mg/kg/day; n = 20). As a positive control, half of the animals on control feed received alendronate subcutaneously. The pullout force of the stainless steel screws was measured after 4 and 8 weeks, and μCT was used to assess bone formation around the PMMA screws. No significant effects of strontium treatment on pullout force were observed, but animals treated with bisphosphonate showed a doubled pullout force. Strontium improved the microarchitecture of the cancellous bone below the primary spongiosa at the growth plate, but no significant effects were found around the implants. Strontium is known to improve bone density, but it appears that this effect is weak in conjunction with metaphyseal bone repair and early implant fixation.

sted, utgiver, år, opplag, sider
Elsevier, 2012. Vol. 50, nr 1, s. 350-356
Emneord [en]
Bisphosphonate; bone; implant; rat; screw; Strontium ranelate
HSV kategori
Identifikatorer
URN: urn:nbn:se:liu:diva-71288DOI: 10.1016/j.bone.2011.10.034ISI: 000299064200045OAI: oai:DiVA.org:liu-71288DiVA, id: diva2:447020
Merknad
funding agencies|Swedish Research Council| VR-2009-6725 |local strategic research project Materials in Medicine||County Council of Ostergotland||Linkopings Universitet, Sweden||Tilgjengelig fra: 2011-10-10 Laget: 2011-10-10 Sist oppdatert: 2017-12-08bibliografisk kontrollert
Inngår i avhandling
1. Improved titanium and steel implants: Studies on bisphosphonate, strontium and surface treatments
Åpne denne publikasjonen i ny fane eller vindu >>Improved titanium and steel implants: Studies on bisphosphonate, strontium and surface treatments
2011 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
Abstract [en]

Purpose: The general aim of this thesis was to increase the understanding of biomaterial surface modifications and local delivery of osteoporosis drugs for bone integration. We therefore (i) characterised and investigated model surface coatings for controlled drug delivery in a rat tibia screw model (ii) elucidated the effect of surface treatment for activation of complement system in vitro.

Materials and methods: Bisphosphonate was immobilised directly to implant surfaces by two methods. In the first method, bisphosphonate was bound via a crosslinked fibrinogen layer to titanium surfaces. In the second method, stainless steel screws were first dip coated in a TiO2 solgel, and thereafter incubated in simulated body fluid (SBF). The so prepared thin calcium phosphate layer on titania bound then bisphosphonate directly with high affinity. The drug release kinetics was determined in vitro by 14C marked alendronate that was quantified with scintillation techniques. The screws were inserted in the metaphysis of rat tibia and the mechanical fixation monitored by screw pullout measurements after 2 or 4 weeks of implantation. In order to compare two different osteoporosis drugs, bisphosphonate and strontium ranelate, stainless steel and PMMA screws were inserted in the tibial metaphysis of rat for 4 and 8 weeks. Bisphosphonate was then delivered subcutaneously and strontium ranelate orally during the whole implantation period. The mechanical fixation was analysed by pullout force measurements, and bone architecture studied by micro-computed tomography (μCT). The immune complement activation on sol-gel- and smooth titanium surfaces was analysed in human blood plasma before and after annealing of titanium at 100-500ºC or upon UVO-treatment for up to 96 hours.

Results: Bisphosphonate coated screws enhanced the screw pull out force after 2 weeks of implantation by more than 30% (fibrinogen coating) and by 93% after 4 weeks (sol-gel derived TiO2 coating). Systemically administered bisphosphonate enhanced the mechanical screw fixation after 4 weeks by more than 96% and after 8 weeks by more than 55% as compared to strontium ranelate treated animals (p = 0.00). Strontium ranelate treatment did not show significant improvement of screw pullout force after 4 and 8 weeks, compared to control. The immune complement surface deposition from blood plasma vanished irreversibly after Ti heat treatment at 250-300 ºC during 30 minutes or after UVO exposure for 24 hours or longer. Tentatively, changes in surface water/hydroxyl binding upon heat- and UVO treatments were observed by XPS and infrared spectroscopy.

Conclusions: The results show that fixation at short implantation time (weeks) of orthopaedic implant can be enhanced by immobilised bisphosphonate on stainless steel or titanium implants. Systemic delivery of strontium ranelate showed no significant effect on implant fixation in rat tibia, and we hypothesise therefore that strontium ranelate will not become a power tool to increase the early implant fixation, but may be beneficial at longer times. Heat annealing or UVO-treatment of titanium surfaces change the surface hydroxylation, leading to decreased immune complement deposition from blood plasma.

sted, utgiver, år, opplag, sider
Linköping: Linköping University Electronic Press, 2011. s. 75
Serie
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 1391
HSV kategori
Identifikatorer
urn:nbn:se:liu:diva-71289 (URN)978-91-7393-085-7 (ISBN)
Disputas
2011-10-07, Planck, Fysikhuset, Campus Valla, Linköpings universitet, Linköping, 10:15 (svensk)
Opponent
Veileder
Tilgjengelig fra: 2011-10-10 Laget: 2011-10-10 Sist oppdatert: 2019-12-19bibliografisk kontrollert

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