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Macroporous microcarriers for introducing cells into a microfluidic chip
Linköpings universitet, Institutionen för fysik, kemi och biologi, Teknisk biologi. Linköpings universitet, Tekniska högskolan.
Percell Biolytica AB, Åstorp, Sweden.
Linköpings universitet, Institutionen för fysik, kemi och biologi, Teknisk biologi. Linköpings universitet, Tekniska högskolan.ORCID-id: 0000-0001-9711-794X
Linköpings universitet, Institutionen för fysik, kemi och biologi, Ytors Fysik och Kemi. Linköpings universitet, Tekniska högskolan. (Transport and Separations Group)ORCID-id: 0000-0002-2773-5096
2014 (engelsk)Inngår i: Lab on a Chip, ISSN 1473-0197, E-ISSN 1473-0189, Vol. 14, nr 18, s. 3502-3504Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Macroporous gelatin beads (CultiSpher™ microcarriers) provide a convenient method for rapidly and reliably introducing cells cultured ex situ into a microfluidic device, where the spheres create a 3D environment for continued cell proliferation. We demonstrate the usefulness of this technique with a proof-of-concept viability analysis of cardiac cells after treatment with doxorubicin. © 2014 the Partner Organisations.

sted, utgiver, år, opplag, sider
Royal Society of Chemistry, 2014. Vol. 14, nr 18, s. 3502-3504
HSV kategori
Identifikatorer
URN: urn:nbn:se:liu:diva-109971DOI: 10.1039/c4lc00693cISI: 000340474300008PubMedID: 25068539Scopus ID: 2-s2.0-84905837163OAI: oai:DiVA.org:liu-109971DiVA, id: diva2:742019
Forskningsfinansiär
Swedish Research Council, 2008-7537 2011-6404
Merknad

Acknowledgements

The primary embryonic cardiomyocytes were provided byJordi Altimiras, Department of Physics, Chemistry andBiology, Linköping University. The authors thank the SwedishResearch Council (Vetenskapsrådet) for fundingviagrants 2008-7537 and 2011-6404

Tilgjengelig fra: 2014-08-29 Laget: 2014-08-29 Sist oppdatert: 2019-01-22bibliografisk kontrollert
Inngår i avhandling
1. Microfluidic biosensor systems for cardiotoxicity assaying
Åpne denne publikasjonen i ny fane eller vindu >>Microfluidic biosensor systems for cardiotoxicity assaying
2015 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
Abstract [en]

Toxicity screening is an important part of pharmaceutical development and early detection of toxic side effects provide the opportunity for early redesign or termination of unfeasible projects. Today toxicity testing is relying on experiments on animals. Ethical concerns, high costs and problems with interspecies variability in animal experiments have introduced incentives for cell-based toxicity assays. The recent development of stem cell technology have raised the hope for toxicity testing with higher predictivity that can reduce the amount of animals sacrificed, increase the patient safety and reduce the costs in pharmaceutical development.

Cell development and behavior is to a large extent dependent on the microenvironment. Microfluidic techniques can be used to build small-sized structures that provide the opportunity to introduce a high degree of control of the cell culture environment with features in cell sizes. In this thesis is demonstrated two different methods for infusing cells into microfluidic cell culture devices using either cells clustered in cardiac bodies during differentiation or cells pre-seeded in microporous carriers prior to infusion.

Microfluidic cell culture devices are well suited for optical  evaluation. Demonstrated in this thesis is fluorescent staining in combination with confocal microscopy as well as automated imaging with evaluation of beating frequency of cardiomyocyte cell clusters can be used to assess toxicity of cells cultured in microfluidic devices.

Biosensors use biological recognition elements to measure the presence of a chemical substance, for example low concentrations of biomarkers secreted by cells in a toxicity assay. Especially capacitive biosensors have shown very low limit of detection. In addition, protein G is demonstrated as an affinity ligand to capture IgG antibodies used as recognition element in a biosensor application or used for antibody screening.

sted, utgiver, år, opplag, sider
Linköping: Linköping University Electronic Press, 2015. s. 49
Serie
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 1678
HSV kategori
Identifikatorer
urn:nbn:se:liu:diva-118295 (URN)978-91-7519-046-4 (ISBN)
Disputas
2015-06-12, Visionen, B-huset, Campus Valla, Linköping, 14:00 (svensk)
Opponent
Veileder
Tilgjengelig fra: 2015-05-26 Laget: 2015-05-26 Sist oppdatert: 2019-01-22bibliografisk kontrollert

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